Okolab H201-UP Manuale utente

H201-UP User Manual

April 2015

www.oko-lab.com

Page1

H201-UP

Compatible with the following XY stages

Compatible with the following Okolab Controllers

XY stage model and serial number must be

specified in purchase order.

H201-T-UNIT-BL

H201-UP User Manual

April 2015

www.oko-lab.com

Page2

Index

1. Components and dimensions................................................................................................... 3

2. Sample Holders................................................................................................................... 4

2.1 Available Sample Holders....................................................................................................... 4

3. Insertion of the Sample Feedback Temperature Sensor.................................................................. 5

4. Insertion of Sample Holder into Chamber ................................................................................... 5

5. Magnetic Locks ................................................................................................................... 7

6. Working with 1x3’’ and 1x2’’ chamber slides - magnetic locks......................................................... 9

7. Connection of the Gas Supply ................................................................................................10

8. Working with Perfusion ........................................................................................................10

9. Connection of the Chamber with stage .....................................................................................11

H201-UP User Manual

April 2015

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Page3

1. Components and dimensions

H301-UP includes the following components:

Chamber base

Chamber body, it owns 12 holes for the insertion of perfusion tubing up to 2.0 mm

Objective adapter, the dimension and type of objective in use must be specified in the purchase order.

Holder, the type of stage on which the chamber will fit must be specified in the purchase order.

Lid, it features a hinge system allowing to open and close the Lid surrounding the objective.

Side View

Chamber top view

Lid

Objective adapter

Holder

Chamber base

Chamber Body

Sample Holder-Order Separately

Figure 1. H201 UP Components and Dimensions.

H201-UP User Manual

April 2015

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2. Sample Holders

2.1 Available Sample Holders

The following sample holders are available.

NOTE: Please contact [email protected] if you cannot find the sample holder you are looking for. We are constantly

adding new inserts to the list.

UP-1x35-M

#1 35mm Petri-dish

UP-14xGS-M

#1 1x3in. chamber slide

UP-1xLABTEK-M

#1 Lab-Tek 1in.x2in. chambered cover glass

UP-1xLABTEK-II-M

#1 Lab-Tek II 1in.x2in. chambered cover glass

UP-1x60-M

#1 60mm Petri-dish

UP-1x35-M

For #1 35mm Petri-dish holder

UP-1x60-M

For #1 60mm Petri-dish holder

UP-1xGS-M

For #1 1”x3” chamber slide holders

UP-1xLABTEK-M

For #1 1”x2” Labtek

chambered cover glass holder

UP-1xLABTEK-II-M

For #1 1”x3”chamber slide and

#2 35mm petri dishes

Figure 2.Available sample holders.

H201-UP User Manual

April 2015

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Page5

3. Insertion of the Sample Feedback Temperature Sensor

Insert the Sample Feedback Temperature Sensor through the dedicated opening located in the H201-UP main body

(see Figure 3, Frontal and 3D views).

1.Frontal view 2.3D view

Temperature Sensor dedicated opening

Figure 3. Insertion of the temperature sensor inside the chamber.

4. Insertion of Sample Holder into Chamber

Sample holders fit into the chamber base and are held in place by magnets embedded within both on the chamber and

on the holder. To introduce a sample holder with the proper orientation, match the red dot on the holder to the one

on the chamber base, as illustrated in Figure 4.

Insert the adapter by

superimposing the red dots

Figure 4. Introduction of the Specimen Holder inside the Chamber Base.

If you want to change the sample holder follow the steps shown in the images of Figure 5 and listed below:

1. Open the lock spring, as shown in Figure 5 Image 2

2. Open the chamber lid as shown in Figure 5 Image 3

3. Remove the chamber lid by lifting it from the workspace, as shown in Figure 5 Image 4

H201-UP User Manual

April 2015

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4. Remove the chamber by lifting it from workspace, as shown in Figure 5 Images 5 and 6

5. Lift and replace the sample holder, as shown in Figure 5 Image 8

Insert the adapter by

superimposing the red dots

Figure 5. Introduction of the Specimen Holder inside the Chamber Base.

H201-UP User Manual

April 2015

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Page7

5. Magnetic Locks

Magnetic locks prevent movement of 35 and 60 mm dishes inside the sample holder.

Figure 6 illustrates the available magnetic locks for 35 and 60 mm dishes. Threaded magnetic posts allow adjusting

holder’s height.

Magnetic Locks for 35 and 60 mm petri dishes.

Included in the corresponding sample holder

Figure 6.Magnetic locks for 35 and 60 mm dish

NOTE: Magnetic locks are included with sample holder.

Spacing rings with variable diameter are also included in according to the needs of the user. Figure 7 shows how to

position the ring inside the sample holder. Ring selection guide diagrams: rings for 35 and 60 mm dishes -Figure 8 and

Figure 9, respectively.

Figure 7. 1) Insertion of the ring, 2) insertion of the 35 mm dish, 3) insertion of the magnetic lock.

NOTE: Spacing rings are included with sample holder.

H201-UP User Manual

April 2015

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Page8

Grainer-Petri35x10 Ibidi μ-Dish35mm-low

Corning-35x10mm MatTek-P35G-X-14-X

Willco-35x10BD Falcon-35x10

Figure 8. Ring selection scheme for 35 mm dish.

MatTek-P50G-X-30-X

Grainer - Petri 60x15

Willco60x15

BD Falcon 60x15

Ibidi μ-dish 50mm-low

Corning-60x15mm

Figure 9. Ring selection scheme for 60 mm dish.

H201-UP User Manual

April 2015

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Page9

6. Working with 1x3’’ and 1x2’’ chamber slides - magnetic locks

Magnetic locks prevent movement of 1’’x 3’’ and 1’’x 2’’ chamber slides inside of the sample holder.

NOTE: Magnetic locks are included with sample holder.

Figure 10. Magnetic lock for 1'' x 3'' chamber slide.

Figure 11. Magnetic lock for 1'' x 2'' chamber slide. To lock the slide, push simultaneously the buttons indicated with (3).

H201-UP User Manual

April 2015

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Page10

7. Connection of the Gas Supply

A single silicon tubing carries output gas from the Okolab Gas Controller to the H201-UP. Silicon tubing connects to a

gas input - brass opening - located on a corner of the H201-UP. See Figure 12. Connect by gently pushing silicon tubing

onto brass opening.

Gas Input

Figure 12.Connection with gas supply.

8. Working with Perfusion

The H201-UP features 12 perfusion holes on the sides for the insertion of perfusion tubing up to 2.0 mm in outer di-

ameter. Small screws plug the perfusion holes when not in use. (Grub screws M3x6). Remove small screws as necessary

before introducing perfusion tubing.

Figure 13 shows the location of perfusion holes.

1.Frontal view 2.3D view

Perfusion dedicated opening

Figure 13. Perfusion

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