Optika IM Series Manuale utente
v 1.0 2017
Model
IM-3F
IM-3FL4
IM Series
INSTRUCTION MANUAL
Page 2
Table of Contents
Warning
Symbols and conventions
Safety Information
Intended use
Overview
Unpacking
Assembling
Using the microscope
Maintenance
Troubleshooting
Equipment disposal
Page 3
Warning
This microscope is a scientic precision instrument designed to last for many years with a minimum of mainte-
nance. It is built to high optical and mechanical standards and to withstand daily use. We remind you that this
manual contains important information on safety and maintenance, and that it must therefore be made acces-
sible to the instrument users. We decline any responsibility deriving from incorrect instrument use uses that does
not comply with this manual.
Symbols and conventions
The following chart is an illustrated glossary of the symbols that are used in this manual.
CAUTION
This symbol indicates a potential risk and alerts you to proceed with caution.
ELECTRICAL SHOCK
This symbol indicates a risk of electrical shock.
Safety Information
Avoiding Electrical Shock
Before plugging in the power supply, make sure that the supplying voltage of your region matches with the
operation voltage of the equipment and that the lamp switch is in o position. Users should observe all safety
regulations of the region. The equipment has acquired the CE safety label. However, users have full responsibil-
ity to use this equipment safely. Please follow the guidelines below, and read this manual in its entirety to ensure
safe operation of the unit.
Intended use
For research and teaching use only. Not intended for any animal or human therapeutic or diagnostic use.
Page 4
Overview
TRINOCULAR
VIEWING
TUBE
(FIXED)
DIOPTRIC
ADJUSTMENT
RING
EYEPIECE
PHASE CONTRAST SLIDER
(OPTIONAL FOR IM-3FL4)
LED
HOUSING
CONDENSER
FILTER HOLDER
COARSE
FOCUS KNOB
FINE FOCUS KNOB
FLUORESCENCE
POWER SUPPLY
TENSION
ADJUSTMENT
COLLAR
OBJECTIVE
STAGE
MICROSCOPE
BODY
LIGHT PATH
SELECTOR
LEVER
GLASS STAGE INSERT
Page 5
Unpacking
The microscope is housed in a moulded Styrofoam container. Remove the tape from the edge of the container
and lift the top half of the container. Take some care to avoid that the optical items (objectives and eyepieces)
fall out and get damaged. Using both hands (one around the arm and one around the base), lift the microscope
from the container and put it on a stable desk.
Assembling
Once you open the box, these are the microscope’s components:
① Microscope body
② Condenser
③ LED illuminator
④ Fluorescence power supply
⑤ Power cables
⑥ Filter holder
⑦ Metal insert for stage
⑧ Glass insert for stage
⑨ Objectives
⑩ Eyepieces
⑪ Fluorescence lters
⑫ Brighteld lters (IF550)
⑬ Orange screen
⑭ Diaphragm assembly
⑮ Mercury lamp house
①
②
⑪⑫⑬ ⑭
⑮
⑩
③
④
⑤
⑥
⑦ ⑧
⑨
Page 6
③
Fig.2
Fig.3
Fig.4
Fig.5
Fig.1
1. Turning the coarse focusing knob ① till the
nosepiece reaches its lowest position.
► For a safe transport, the nosepiece is
placed in the lowest position and the
tension adjustment collar ②is adjust-
ed to the appropriate tension when the
microscope leaves the factory. (Fig.1)
2. Screw the lowest magnication objective
on to the turret from the right side, then
turn the turret clockwise. Mount the other
objectives in the same way, following the
sequence from low to high.
► Note: the objectives can also be in-
stalled through the stage opening.
(Fig.2)
► Clean the objectives regularly. In inverted
microscopes, the objectives are very sen-
sitive to dust.
► To prevent dust and contamination from
entering the microscope, cover all the un-
used holes with dust caps ③. (Fig.3)
► When operating, use the low magnication
objective (10X) to search and focus the
specimen, then switch to higher magnica-
tions.
► When switching between objectives, slowly
turn the nosepiece until it clicks. The click
means that the objective is in the right posi-
tion, in the center of the light path.
①
②
Installing the objectives
The stage extension can be installed on either
side of the stage to enlarge the working sur-
face. The mechanical stage must be installed
on the side opposite the extension.
For right-handed operators, the mechanical
stage is normally installed on the right side.
1. Installing the stage extension: Screw the
bolts on to the extension, then mount the
extension from below the stage. (Fig.4)
2. Installing the mechanical stage: As for the
extension, the mechanical stage is xed
with two bolts under the stage. (Fig.5)
Installing the stage extension and
the mechanical stage (OPTIONAL)
Page 7
Page 7
1. When using the glass stage, make sure
that the insert is horizontal.
2. Install the stage insert in the stage open-
ing. (Fig.6)
Installing the stage insert
Installing the eyepieces
Insert both eyepieces into the tubes of the
optical head. (Fig.7)
Fig.6
Fig.7
Fig.8
Fig.9
Fig.10
1. Insert the condenser illumination unit into
the bracket. (Fig.8)
2. Turn the condenser illumination unit clock-
wise about 90°, with the “AS” mark of lter
holder facing forwards. Align the screw of
the condenser illumination unit and the
hole of the holder, then screw the bolt in
the hole with the supplied allen wrench.
(Fig.9)
3. Insert the connector plug into the connec-
tor jack.
4. Push the LED housing gently into the
holes of the illumination unit. (Fig.10)
Installing the condenser
illumination unit and the LED
housing
Page 8
► Remove the lter holder, then install the
color lters you need. (Fig.11, Fig.12)
Mount the color lter at as shown in ①,
verifying that they are not tilted.
► If the color lter is tilted or otherwise
out of place ②, it may fall.
The color lters can be stacked in the
holder. This allows to install as many lters
as needed, as long as the whole thickness
is less than 11 mm.
• Pull the black plastic cover out, from the
microscope rear. (Fig.13)
• Insert the lens/diaphragm assembly from
the back. In order to ease the insertion,
just tilt the assembly at about 45° and
move it forward. Fix it using the 3 provided
allen screws. (Fig.14)
• Insert the lamp house and x it with the al-
len screw (already inside the support tube
(1) ). (Fig.15)
Installing the color lters
Installing the uorescence
Fig.11
Fig.12
Fig.13
Fig.14
Fig.15
①
②
Page 9
• Insert the lter holder into its slot near the
lamp house. (Fig.16)
• Unscrew the protection cover on the left
side of the microscope, with the provided
allen wrench. (Fig.17)
• Slide the green lter inside the dovetail
guide, after screwing the lter lever into the
threaded hole of the lter. Mount the pro-
tection cover back in its position. (Fig.18)
• On top of the lter lever, screw the terminal
with the etched G. Repeat the same steps
for the right side, mounting the Blue lter-
set. (Fig.19)
• For preventing possible damages from
UV radiation, mount the orange protection
screen as shown. (Fig.20)
Fig.16
Fig.17
Fig.18
Fig.19
Fig.20
Page 10
• Connect the cable from the external power
supply to the HBO lamp house. (Fig.21;
Fig.22)
• Connect the power cable to the external
power supply. (Fig.23)
Fig.21
Fig.22
Fig.23
• The input voltage for uorescence power supply is 110-240Vac.
• Please use the standard power cable provided by our company. Select suitable one when mis-
sing or damaged.
• Connect the power supply correctly, be sure to have a good earth connection.
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