OPTO-EDU A11.6603 Manuale utente

A11.66 03

Biological Microscope

Instruction Manual

CONTENTS

Attentions …………………………………………………2

1. Application ……………………………………………3

2. Specifications …………………………………………4

3. Installation and use ……………………………………4

4. Maintenance ……………………………………… 10

5. Troubleshooting ………………………………………13

ATTENTIONS

1. Disassembly only by the professionals

The microscope has been adjusted before shipping, Unprofessional-person

should not disassemble and remove any other parts. Disassemble and remove

any other parts will result in equipment damage .

If you have any questions, please contact with manufacturer or local

distributor.

2. Note the input voltage if correspond

This instrument designed for wide input voltage (100V~240V,50/60HZ),

applicable to most area. But if the supply voltage exceeds this range, the

instrument will be seriously damaged.

3. Prevent burns and fire

When using power equipment, bulbs and collecting mirror and other nearby

parts of the set will rise sharply in temperature until it reaches a thermal

equilibrium state. Pay attention to anti-hot logo, they should be careful not be

burn when in use.

Alcohol, gasoline, paper and other flammable materials can’t near the

lamp in case of fire.

4. Notes on replacing the bulb

Replacement should be based on the identity of the instrument using the same

specifications of the bulb (3.4V/3W LED or G4 12V/20W halogen), otherwise

it may cause equipment damage.

The power supply must be cut off before bulb replacement, the bulb must be

cooled off completely before proceeding! Don’t left fingerprints,dust or oil on

the bulb.

5. Carry and operation

Power must be cut off before moving. Be careful not to crush your finger

when placed.

This instrument is a precision instrument, and it should be handle with care, severe

shock can cause serious damage to equipment-related parts.

The required available environment for using of the equipment:

Indoor temperature: 0 ℃ ~ 40 ℃ Maximum relative humidity: 85%

High temperature or high humidity may cause mildew, fog or dew of the

optical components, and make the instrument not work.

6. This eletrical instrument don’t cause electromagnetic interferernce for other

device.

Fig.1 B Series Microscope Outside Drawing

1. Application

B series biological microscope is designed for teaching, clinical verify.

The series microscope with original style, steady structure, convenient

operation and clear image is suitable for observing various biological

specimens, and mostly applied in school, college, hospital, etc.

Binocular head

Nosepiece

Objective

Clamps

Coarse & fine

focusing

unit

Power supply

base

Main body

Eyepiece

Monocular head

Condenser

Stage

Aperture diaphragm

lever

Stage up-down

knob

Collector

Power switch

2. Specifications

Item B series

Optical system

Finity

Mechanical

tube length 160mm

Total

Magnification 40X～1600X

Objective

Category Achromatic, plan achromatic, semi-plan

Magnifying

power Based on the standard GB/T 2609.

Eyepiece

Magnification

10X. 16X（Optional）

Obervation

method Binocular. Monocular (Optional）

Observation

tube

Binocular . Monocular（Optional）,

Trinocular（Optional）

Eyepiece and

tube size φ23.2 8

Nosepiece Quadruple

Condenser Abbe

Stage Mechanical stage,Fixed stage（Optional）

Focusing Unit Fine adjusting unit, division 0.002mm.

Illumination Mirror（Optional）, 3W LED, 12V20W Halogen（Optional）

Accessory Photomicrographic device, dark-

field illumination unit,

microplotter.

3. Installation and use

3.1 Installation

B series microscope with whole package is convenient for installation.

Open the package carton, and insert eyepiece into the eyepiece tube of the head

(eyepiece for monocular microscope has been inserted before packing). If

objectives don’t be fixed, please take objectives out from objectives packing box,

and drive them into the holes of nosepiece orderly and tightly according to the

multiplying power in order.

Take out the oil bottle and dust cover, and place it properly for convenient use.

▲Please totally take out the devices in the packaging box.

▲Please don’t throw package waste away freely to protect the enviroment.

3.2 Use

3.2.1 Power Supply

Make sure the supply voltage meets the instrument’s requirement, then plug

in the socket. Rotation potentiometer with switch used by the instrument is

for protecting lamp, in case high electric current shock , prolong service time.

3.2.2 Place sample（Fig.2）

 Please place specimen on the stage, and the face with cover glass should

be up, then clip it with clamp.

 Clipping specimen should be careful in order to avoid spoiling glass. Place

specimen on the stage levelly.

 Making the specimen into optical path by adjusting vertical moving knob

and horizontal moving knob.

3.2.3 Condenser（Fig.2）

1．Condenser up-down

Turn the condenser up-down knob to adjust the distance between condenser

front and the specimen, and change the equal illumination to obtain the best

brightness.

2. Aperture diaphragm adjustment

Rotating the handle of aperture diaphragm of condenser to adjust diaphragm

size can adjust the contrast of the specimen.

In general, aperture diaphragm can be adjusted to 70% or 80% of objective

exit pupil diameter , will get good image with best contrast.

3.2.4 Adjustment of interpupillary distance and diopter (Fig.3)

1. Adjustment of Interpupillary Distance

Please adjusting binocular tube to make left and right field coincide completely .

▲Interpupillar distance is different for everyone, so interpupillar distance

should be adjusted before using binocular microscope.

2. Adjustment of diopter

As focusing for binocular, user should observe right eyepiece with right eye, and

make the right eyepiece clear by focusing adjustment, then observe the left

Fig.3 Adjustment of interpupillary

distance and diopter

Fig.2 Stage and condenser

Clamps swtich knob

Specimen

Stage

vertical

adjusting

knob

Stage

horizonal

adjusting

knob

Condenser

adjusting

handwhee

Aperture

diaphragm

lever

Diopter

adjusting

ring

nterpupillary

distance dial

Eyepiece

Left side

Right side

eyepiece, at the same time, adjust the diopter ring of the left eyepiece

tube(diopter difference compensation)to make the image of left eyepiece clear as

same as the right eyepiece.

3.2.5 Focusing unit（Fig.4）

Usually 10X objective with large field of view and long depth of field should

be used for focusing adjustment first.

Take 10X objective into bright path, then observe the specimen with right

eyepiece by right eye and turn coarse focusing knob to find the image, then turn

slowly fine focusing knob to make the image clear.

All objectives of our microscope are precisely adjusted, and approximate

clear image should be found with the other objectives after getting the clear

image with 10X objective. If the image isn’t enough clear with the other

objectives, please turn fine focusing knob slightly to find the satisfactory image.

▲Don’t rotate the left & right coarse and fine focusing knobs in reverse

direction at the same time, if so, the focusing system will be damaged.

▲Don’t directly pull objective to turn nosepiece when user changes the different

objectives,if so, optical quality of microscope possibly be affected.The right way

Fig. 4 Coarse & fine focusing knobs and tension adjusting knobs

Right coarse

focusing knob

Left fine

focusing knob

Left coarse

focusing

knob

Tension

adjusting knob

Right fine

focusing knob

is to take the tooth-like part of the nosepiece to turn it, and make the objective

into correct position and into the bright path.

3.2.6 Use of tension adjustment knob (Fig.4)

The tension adjustment ring can adjust the tension of the coarse and fine

focusing unit to prevent the stage from sliding down automatically and to

improve the comfort of operation. Rotation clockwise makes tension decrease,

and by contraries, rotation counterclockwise makes tension increase.

3.2.7 Use of oil immersion objective

The 100X objective can be used for observation even if without immersion oil.

But little non-synthetic resin immersion oil or distilled water between the

objective and specimens will fully grasp the objective function , bubbles and

impurities can’t in immersion oil which will affect image.

after adjusting with the 40X objective, adding moderate immersion oil

between the front lens of 100X objective and the cover glass of specimen can

make the image clearer. Please pay attention that air bubble and impurity can’t

be in the immersion oil, otherwise, the image would be affected.

▲After immersion oil used, the oil of specimen and the microscope surface

should be cleaned with absorbent cotton, lens paper, gauze or soft cotton cloth

with moderate mixture of pure industrial alcohol and ether (proportion 1:4)

▲Standard thickness 0.17mm cover glass should be chosen when high times

objective used, and thickness error should be within 0.01mm, otherwise, image

definition wouldbe affected.

3. 2.8 Use of powersupply

Plug the power line in base socket（Fig.5），plug the other end of power line

into power socket. Adjusting LED lamp brightness by slide the base switch.

Fig.5 Replacement of fuse

3. 2.9 Reflector （Fig.6）

Reflector is optional accessary ,only use without electricity provided.

When use 100X objective ,not recommend reflector for illumination.

Carefully remove the collector from the base and connecting the

reflector with the base.

Turning the reflector around the vertical axis so that the reflective side will

face to the light direction.At the same time around the central axis to rotate the

reflector, reflecting surface and horizontal plane is roughly 45°angle,making the

light totally shining on the specimen.Concave mirror is suitbale for large area

outdoor lighting source ,such as bright sky etc.

3. 2.10 Cautions after use

 Power of microscope should be turned off after using(turning

potentiometer with click voice means be closed）, and the plug should be

Fig.6 Reflector

Potentiomer

Socket

Fuse holder

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