Q Imaging Retiga ELECTRO Manuale utente

USER MANUAL

DIGITAL IMAGING MADE EASY

www.QIMAGING.com

RETIGA ELECTRO™USER MANUAL

Applicability

This document applies to the QImaging Retiga ELECTRO camera. For the latest updates, please visit

www.qimaging.com.

Notice of Copyright

document is prohibited.

Trademarks and Proprietary Names

QImaging and Retiga ELECTRO are trademarks of QImaging. PVCAM® is a registered trademark of Roper

Scientiﬁc, Inc. Product names mentioned in this document may be trademarks or registered trademarks of

QImaging or other hardware, software, or service providers and are used herein for identiﬁcation purposes

only. Microsoft® and Windows® are registered trademarks in the U.S. and other countries of Microsoft

Corporation and are used herein for identiﬁcation purposes only.

QImaging Corporation Address Information

19535 56th Avenue,

Suite 101

Surrey, BC, Canada V3S 6K3

+1.604.530.5800

www.qimaging.com

QImaging Technical Support

Technical support is available to all registered users of QImaging products from 6am to 5pm Paciﬁc Standard

Time.

[email protected]

+1.800.874.9789

http://www.qimaging.com/support/contact

RETIGA ELECTRO™USER MANUAL

QIMAGING LIMITED WARRANTY

Standard Product Warranty Plan

A Standard Product Warranty Plan is included with every QImaging camera purchase. This Warranty Plan

includes parts and labor for two full years (starting from the shipping date of the camera). The Standard

Product Warranty Plan is provided on all new and used equipment, including retired demonstration cameras.

Extended Product Warranty Plan

Extended Product Warranty Plans are reasonably priced and very easy to purchase. Available for all QImaging

cameras currently under warranty, the Extended Product Warranty Plan includes parts and labor, and is

available for an additional three-year period to extend your warranty to ﬁve years.

When you purchase an Extended Product Warranty Plan from QImaging, you are assured of our commitment

to minimizing down times. Your needs are our top priority and QImaging responds immediately. The QImaging

Service and Support team is focused on expediting all customer requests to provide a fast and complete

solution.

QImaging also offers on-site training as well as online operational training programs. These programs are

designed to get you up and running with your new camera quickly and efﬁciently.

Contact a QImaging Representative to learn more about Extended Product Warranty Plan options.

RETIGA ELECTRO™USER MANUAL

Table of Contents

INTRODUCTION ............................................................................................................. 1

System Components .........................................................................................................1

Precautions ........................................................................................................................2

Environmental Conditions for Operation and Storage.........................................................2

Optics and Mounting .........................................................................................................2

Cleaning ............................................................................................................................3

Repair ................................................................................................................................3

INSTALLATION ............................................................................................................... 4

Host Computer Requirements ............................................................................................4

Camera Power Requirements .............................................................................................5

Install the Camera Driver .................................................................................................... 5

Connect the Camera..........................................................................................................5

Note on Selecting the Optimal USB 3.0 Port ....................................................................... 6

Note on Optimum Mounting of Retiga ELECTRO Camera...................................................8

USING YOUR RETIGA ELECTRO CAMERA ..................................................................... 9

Imaging Software...............................................................................................................9

Basic Camera Parameters ...................................................................................................9

Exposure Time....................................................................................................................9

Gain State..........................................................................................................................9

Sensor Cooling.................................................................................................................10

Clearing Mode.................................................................................................................10

ROI / Binning....................................................................................................................11

Device Synchronization ...................................................................................................11

Trigger-First Mode ............................................................................................................ 11

Bulb Mode.......................................................................................................................12

Recommended Initial Settings .........................................................................................13

Embedded Image Processing Algorithms ..........................................................................14

Defective Pixel Correction................................................................................................. 14

TROUBLESHOOTING .................................................................................................... 15

Resolving Problems with the Camera................................................................................ 15

Unresolved Problems - Contacting QImaging Support ...................................................... 17

SPECIFICATIONS ........................................................................................................... 18

RETIGA ELECTRO™USER MANUAL

CHAPTER 1

INTRODUCTION

The Retiga ELECTRO is the ideal camera

for highly sensitive microscopic imaging

and documentation. With peak quantum

efﬁciency of 75%, combined with

extraordinarily low electronic and thermal

noise, the Retiga ELECTRO provides higher

resolution and sensitivity in a form factor

that saves space in laboratory settings.

The Retiga ELECTRO camera uses a USB

3.0 data connection for a fast and easy

installation, while maintaining backwards

compatibility for USB 2.0 at reduced

data rates for use with a variety of host

computers.

This manual describes the installation and

conﬁguration procedures for your new

Retiga ELECTRO. Descriptions of Retiga

ELECTRO camera settings are also included.

System Components

Your new camera system includes:

■Retiga ELECTRO Camera

■7.5V DC, 2.5A power supply (actual model may vary)

■USB 3.0 cable

■Optional Ocular®Software

■USB ﬂash drive: PVCAM driver, manual

RETIGA ELECTRO™USER MANUAL

Precautions

The Retiga ELECTRO system electronics are extremely sensitive to electrostatic discharge (ESD). To avoid

permanently damaging the system, please observe the following precautions:

■If you are using high-voltage equipment (such as an arc lamp) with your camera system, be sure to

turn the camera power on last and power the camera off ﬁrst.

■Never connect or disconnect any cable while the camera system is powered on.

■Although you should turn off the camera’s power supply before disconnecting any camera system

cable, you do not need to power off your computer to detach the cables.

■Use caution when triggering high-current switching devices (such as an arc lamp) near your system.

The image sensor can be permanently damaged by transient voltage spikes. If electrically noisy

devices are present, an isolated, conditioned power line or dedicated isolation transformer is highly

recommended.

■Always leave at least one inch of space around the camera housing.

■Never open the camera. There are no user-serviceable parts inside the Retiga ELECTRO camera.

Opening the camera voids the warranty.

■Use only the USB 3.0 interface, cables, and power supply designated for this camera system. Using

non-Retiga ELECTRO cables or power supplies may result in permanent damage to your system.

■Do not use a C-mount lens that has optics that extend behind the ﬂange of the lens.

Environmental Conditions for Operation and Storage

Your Retiga ELECTRO camera system should be operated in a clean, dry environment. The camera system’s

ambient operating temperature is 0°C to 30°C with 80% relative humidity, noncondensing. Contact QImaging

customer service for information if operating outside of this range.

To protect your Retiga ELECTRO during storage, use its original containers. To protect the system from excessive

heat, cold, and moisture, store at an ambient temperature between -20°C and 60°C with a relative humidity of

0% to 90%, noncondensing.

Optics and Mounting

The Retiga ELECTRO camera lens mount accepts a standard 1” diameter, C-mount lens or adapter. The camera

is also compatible with a standard C-mount lenses as long as its optics do not extend behind the ﬂange of the

lens.

If desired, the Retiga ELECTRO may be mounted to a tripod or other device using the mounting attachment

located on each side of the camera’s front plate. See the Speciﬁcations chapter for more information.

RETIGA ELECTRO™USER MANUAL

Cleaning

Clean the exterior surfaces of the camera with a dry, lint-free cloth. To remove stains, contact QImaging

Support. To clean the camera’s imaging window, use only a ﬁltered compressed-air source. Handheld cans are

not recommended, as they may spray propellant onto the window. Do not touch the window.

Repair

The Retiga ELECTRO contains no user-serviceable parts. Repairs must be done by QImaging. Should your

camera system need repair, contact QImaging Support. Please save the original packing materials so you can

safely ship the camera system to another location or return it for repairs if necessary.

IMPORTANT: DO NOT OPEN the camera. Opening the Retiga ELECTRO camera voids the warranty.

RETIGA ELECTRO™USER MANUAL

CHAPTER 2

INSTALLATION

This chapter will detail the proper installation of your Retiga ELECTRO camera. In order to install and use the

camera, the following items are required:

■Retiga ELECTRO camera (supplied)

■USB 3.0 data cable (supplied)

■7.5V DC, 2.5A power supply (supplied)

■USB PVCAM Driver “installer” (supplied and also available on the QImaging website)

■110V/220V power connection

■A PVCAM compatible imaging application, such as Ocular (optional)

■A PC that meets host computer requirements

Optional items that are included with your Retiga ELECTRO camera:

■Trigger cable

Host Computer Requirements

The host computer for your Retiga ELECTRO camera must include:

■Windows 7, Windows 8 or Windows 10 operating system (64 bits)

Refer to the QImaging website for the latest list of minimum computer recommendations

■2.0 GHz or faster Intel processor: either Xeon or Core i5

■4+ GB RAM

■250+ GB serial ATA (SATA) HDD and/or >64 GB solid state drive (SDD) for high-speed imaging and

storage

■256+ MB slot-based ATI/NVIDIA video graphics card (i.e., not an “onboard/integrated graphics”

adapter)

■USB port or Internet access to install the driver

■At least one USB 3.0 interface port (USB2.0 is supported, but at reduced frame rate)

NOTE: Minimum requirements as of December 2015. Supported computer systems will change as new

cameras, computer hardware, and operating systems are introduced and older models then become obsolete.

For current information on recommended computer speciﬁcations, please visit:

http://www.qimaging.com/support/computer-specs

NOTE: The Retiga ELECTRO camera will also operate from a USB2 port by reducing the maximum readout

speed from 50MHz to 17.5 MHz, impacting achievable frame rates.

RETIGA ELECTRO™USER MANUAL

Camera Power Requirements

■7.5V DC, 2.5A

A power supply for use with your Retiga ELECTRO camera has been provided by QImaging.

IMPORTANT: Follow the below steps in order.

DO NOT CONNECT the camera until the driver is installed.

1. Install the Camera Driver

In order for the Retiga ELECTRO camera to communicate with the host PC, the camera’s device driver must ﬁrst

be installed. The latest version of the PVCAM driver installer is available on the QImaging website as well as the

provided USB drive. The recommended driver for the Retiga ELECTRO is PVCAM 3.6.7.2 or newer. However,

the camera will work with revisions as old as PVCAM 3.0.9.2. Be sure to select the appropriate installer based

on your PC’s operating system.

The Retiga ELECTRO camera is designed and tested for use with Microsoft Windows 64-bit operating systems.

Contact QImaging customer service for more information.

After completing the PVCAM installation, restart your PC when prompted by the wizard. For advanced users

wishing to use the camera in a custom programming environment, the PVCAM Software Development Kit is

available on the QImaging website.

NOTE: Supplemental PVCAM installation information can be found on the QImaging website:

http://www.qimaging.com/support/downloads/

Note: Users can ﬁnd the PVCAM SDK is on the QImaging website at:

http://www.qimaging.com/support/downloads/pvcam-sdk/

2. Connect the Camera

Note: Power switch on power supply cable

There are three connectors on the back of the Retiga ELECTRO camera: a USB 3.0 connector, a power supply

connector, and an I/O connector. Connect the camera following these steps

1. Connect the supplied USB 3.0 cable to the USB 3.0 interface port on your computer and connect

the other end of the cable to the USB 3.0 connector located on the back of the camera. (The USB

3.0 interface transfers camera settings and image data to the host PC.) The Retiga ELECTRO camera

RETIGA ELECTRO™USER MANUAL

should be connected to a USB 3.0 port. It should also be the only device on its USB root hub

controller (see Figure 2 below). Please see the following section on how to select the appropriate

USB port for optimum camera performance. This ensures the maximum bus bandwidth is available

to the camera for data transfers. USB 3.0 ports are often (but not always) identiﬁable by a blue

internal socket or the “SuperSpeed” (or just “SS”) USB logo.

2. After the USB 3.0 cable has been connected to the computer and camera, connect the Retiga

ELECTRO camera power supply’s cable to the power connector located on the back of the camera.

(Your Retiga ELECTRO camera is powered by the 7.5V 2.5A or greater power supply provided by

QImaging.) Lastly, plug the power supply’s cord into an appropriate power source.

3. Optional: An optional I/O connector is available on your camera for optional hardware triggering.

The I/O connector provides multiple I/O signals that allow highly precise synchronization with

external hardware components such as light sources via TTL signals. Information on how to set

up and conﬁgure the Retiga ELECTRO camera for hardware triggering can be found in the next

chapter of this manual.

Note on Selecting the Optimal USB 3.0 Port:

Most modern computers come with both USB 3.0 and USB 2.0 ports. This camera should be connected to a

USB 3.0 port. However, if no USB 3.0 port is available, you can operate the camera with USB 2.0 as well. The

camera is designed to work with USB2.0, at a reduced data rate of 17.5MHz pixels/sec.

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