Serva BlueLine BlueSpin Cryo Manuale utente

BlueLine

Instruments for Electrophoresis

SERVA Electrophoresis GmbH - Carl-Benz-Str. 7 - -69115 Heidelberg

Phone +49-6221-138400, Fax +49-6221-1384010

e-mail: [email protected] - h p://www.serva.de

INSTRUCTION MANUAL

___________________________________________________________________

BlueSpin Cryo

(Cat. No. BS -CRYO)

Index

1 Meanings of Symbols & Safe y Precau ions ........................................................ 2

1.1 Symbols on he device .................................................................................. 2

1.2 Safe y Precau ions ........................................................................................ 2

2 Packing Lis & Technical Specifica ions ............................................................... 4

2.1 Packing Lis ................................................................................................... 4

2.2 Technical Specifica ions ................................................................................ 4

3 Ins alla ion ............................................................................................................ 5

4 Opera ion ............................................................................................................. 8

4.1 Con rol panel ................................................................................................. 8

4.2 Se ing RPM / RCF ........................................................................................ 9

4.2.1 Se ing RPM ............................................................................................ 9

4.2.2 Se ing RCF ............................................................................................ 9

4.3 Se ing Time ................................................................................................ 10

4.4 Se ing Tempera ure and Fas Cool ............................................................. 10

4.4.1 Se ing Tempera ure ............................................................................. 10

4.4.2 Fas Cool............................................................................................... 11

4.5 Accelera ion / Decelera ion .......................................................................... 11

4.6 Program Saving & Recalling ........................................................................ 12

4.6.1 Saving ................................................................................................... 12

4.6.2 Recalling ............................................................................................... 12

4.7 S ar /S op .................................................................................................... 12

4.8 Pulse ........................................................................................................... 13

4.9 Emergency Door Open ................................................................................ 13

4.10 Fuse replacemen ........................................................................................ 13

5 Main enance ...................................................................................................... 14

5.1 Ou er par of Ins rumen .............................................................................. 14

5.2 Chamber ...................................................................................................... 14

5.3 Shaf ............................................................................................................ 14

5.4 Ro or ............................................................................................................ 14

5.5 Transpor a ion of Ins rumen ....................................................................... 14

6 Trouble Shoo ing ................................................................................................ 15

6.1 Check Lis .................................................................................................... 15

6.2 Error code .................................................................................................... 16

7 Ro ors and Accessories ..................................................................................... 18

Vers. 04/13

1 Meanings of Symbols & Safety Precautions

1.1

1.11.1

1.1 Symbols on the device

Symbol Meaning Symbol Meaning

Indica e a hole for

manual door

opening in case

of emergency

A en ion and

warning for

elec ric shock

A en ion and

warning for ro or

coupling.

A en ion and

warning for door

opening and

closing

A en ion and

warning for

correc way of

sample

balancing in he

ro or.

1.2 Safety Precautions

Before using he ins rumen , please read his opera ion manual o ensure correc

usage hrough unders anding. Incorrec handling of he ins rumen could possibly

resul in personal injury or physical damage on he ins rumen or i s accessories.

• ALWAYS loca e he ins rumen on a fla , rigid and s able able capable of

wi hs anding he weigh of he ins rumen and i s spinning opera ion.

• ALWAYS make a safe y zone of 30 cm around he cen rifuge o indica e ha

nei her hazardous ma erials nor persons should be permi ed wi hin he area

during opera ion.

• ALWAYS posi ion he ins rumen wi h enough space on each side of

ins rumen o ensure proper air circula ion.

• ALWAYS ins all he ins rumen wi hin a empera ure and humidi y con rolled

environmen . (Permissible ambien empera ure: +5 °C ~ +35 °C, Rela ive

humidi y: ≤ 85 %)

• Before connec ing he power, check he ra ed vol age.

• Unapproved ro ors and associa ed accessories should no be used.

• Before opera ing he ins rumen , check if he ro or and he ro or lid are

securely fas ened.

• The ins rumen should be opera ed wi h a ro or properly ins alled and secured

o he mo or shaf .

• Moun he ro or on he mo or shaf properly, check i wi h spinning manually.

• Do no s op he ro or by ouching wi h hand during he ins rumen is running.

• Emergency door open should be performed only when spinning is comple ely

s opped.

• The ra ed speed or specific gravi y should no be exceeded. Samples whose

densi y is grea er han 1.2 g/ml mus have reduced maximum ro a ional speed

o avoid ro or failure.

• The sample con en should no exceed 80 % of o al capaci y of a ube.

O herwise, i would cause spillage of sample fluid and even he ube breakage.

• ALWAYS load he ubes symme rically wi h evenly weigh ed samples o avoid

ro or imbalance. If necessary, use he wa er blank o coun erbalance he

unpaired sample.

• The opera ion speed should no exceed he highes value of he individual

guaran eed g-forces of each cen rifuge, ro or, bucke or adap ors and sample

con ainer, especially he guaran eed g-force of sample con ainer should no be

neglec ed.

• The ro ors should be cleaned and kep dry af er every use for longer life and

safe y.

• ALWAYS disconnec he power supply prior o main enance care and service

o avoid elec rical shock.

• ALWAYS use proven disinfec ion procedures af er cen rifuging biohazardous

ma erials.

• Flammable, oxic, radioac ive, explosive, or corrosive ma erials should no be

cen rifuged.

• When i is necessary o use oxic or radioac ive ma erials or pa hogenic micro-

organisms which belong o he Risk Group II of WHO: “Labora ory Bio- safe y

Manual,” should follow na ional regula ions.

Attention

• o not place dangerous materials within 30 cm distance around the

instrument, and that is also recommended by IEC 61010-2-020.

• Use the emergency door open function only when the door button on the

control panel is dumb under the condition of complete stop of rotor

running.

• Never try to open or move the instrument if it is not completely stopped.

• If the power input is more than +/- 10% of the recommended voltage or

fluctuates frequently, it may cause malfunction of the instrument and

often result in serious damage.

• Install the instrument at a place without any kinds of corrosive gases.

2 Packing List & Technical Specifications

2.1 Packing List

• Microcen rifuge

• 6 mm Wrench

• Ro or Locking Tool

• Emergency Open Tool

• Power cord

• Manual

2.2 Technical Specifications

Max. RPM / RCF 17,000 rpm / 27,237 xg

Max. capaci y 30 x 2.0 ml ubes 8 x 8- ube PCR s rips

Temp. range (°C) -20 ~ +40

FAST COOL bu on Yes

Time con rol Pulse, imed < 100 min or con inuous

RPM / RCF conversion Yes

Noise level ≤ 56 dB

Acc / Dec 9 / 10 s eps

Program memory 100

Ro or Iden ifica ion Au oma ion

Imbalance cu ou Yes

Display Blue LCD

Safe y lid lock Yes

Power supply 220 V / 50~60 Hz

Power requiremen 2 kVA

Dimension (W x D x H) 310 mm x 620 mm x 265 mm

Weigh wi hou ro or 43 kg

3 Installation

1. Connec he AC Power cord o he power socke on

he back of he ins rumen .

2. Turn on he ins rumen by pressing he swi ch on he

back of he ins rumen .

3. For opening he door, press he [DOOR] bu on.

4. Close he door un il hearing a clank shu .

5. When he door is opened, he door LED urns on.

When he door is closed, he door LED urns off.

• If he door is opened, he ins rumen could no be opera ed even wi h

pressing he [S ar ] bu on.

• For opera ional safe y, his ins rumen has an au oma ic ro or recogni ion

func ion.

• When you supply he power, he display shows "Searching Ro or" / “Change

o Ro or ID”. If he ro or is absen , “Error 9” will be displayed. This message

will be cleared af er ro or coupling and running.

• The door is no au oma ically opened af er finishing opera ion o keep he

sample a proper empera ure.

• Power Failure: If here is any power failure during opera ion, he door can no

be opened by using he [Door] bu on. The door can be opened only when

he opera ion is comple ely s opped and he power is on again. If you wan o

open he door during he power failure, please refer o chap er: “Emergency

Door Open”.

6. Before coupling a ro or, clean he mo or shaf and chamber wi h sof dry owel.

7. Moun a proper ro or in o he mo or shaf .

8. Grasp he ro or wi h one hand, and place Ro or

Locking Tool a he cen er hole of he ro or.

To assemble he ro or: Ro a e he Ro or Locking

Tool clockwise un il igh ly assembled.

To disassemble he ro or: Ro a e he Ro or Locking

Tool coun erclockwise

9. To close he ro or lid, ro a e he ro or lid nu

clockwise. For opening lid: ro a e he ro or lid nu

coun erclockwise.

When you run a fixed angle ro or, make sure ha he

ro or lid is igh ly closed. If you don’ close he ro or

lid comple ely, i will be crushed.

10. Posi ioning of sample ubes:

• Before loading sample ubes, check for wa er drops or dir in he ro or hole

or inner adap or.

• If here is a wa er drop or dir in he ro or hole or inner adap or, remove i

wi h sof dry clo h.

• Tubes should be placed in he ro or wi h same amoun of samples a

symme rical posi ions.

• Only use appropria e cen rifugal ubes and do no exceed he speed beyond

he ube’s max g-force.

• For safe y, fill he sample for 70 ~ 80 % in he ubes.

• If he number of samples is no in pair, please load he con rol ubes a each

symme rical posi ion. O herwise, i resul s noise and vibra ion, and

even ually damages he ins rumen .

Correct Ways of Sample Balancing & Tube usage

4 Operation

4.1 Control panel

Button

Function

RPM /

RCF For au oma ic conversion of RPM / RCF and o se he speed

TIME Use o se ime, available range up o 99 min 59 sec (00:00: con inuous)

Temp Use o se empera ure (-20 °C ~ 40 °C)

ACC / DEC

Use o se he accelera ion & decelera ion level from 1 o 9 s eps. ‘0’ in

decelera ion s ep means na ural decelera ion. Larger number means

fas er accelera ion or decelera ion.

PROG Use o save a se of se ing values or recall he saved program number

Fas Cool Use o reach rapid refrigera ion up o he se ing empera ure.

Pulse Use for quick runs

En er Use for comple ion of da a se ing

S ar /S op Use o s ar and s op opera ion

Door Use o open ins rumen lid

4.2 Setting RPM / RCF

4.2.1 Setting RPM

1. Speed se ing uni : 10 rpm or 100 rpm

RPM MODE is genera ed by pressing he [RPM /

RCF] bu on once.

RPM LED is flickering on he display window.

2. Press he [▲▼] bu ons o change inpu value.

Af er 5 seconds from ouching he [▲▼] bu ons, he

uni of se ing value is changed from 10 rpm o 100

rpm. If you do no ouch he [▲▼] bu on for 5

seconds, he se ing mode is cleared.

Touch he [En er] bu on o save and comple e he

se ing.

4.2.2 Setting RCF

1. Speed se ing uni : 1 rcf or 10 rcf

RCF MODE is genera ed by pressing he [RPM /

RCF] bu on wice.

RCF LED is flickering on he display window.

2. Press he [▲▼] bu ons o change inpu value.

Af er 5 seconds from ouching he [▲▼] bu ons, he

uni of se ing value is changed from 1 rcf o 10 rcf. If

you do no ouch he [▲▼] bu on for 5 seconds, he

se ing mode is cleared.

Touch he [En er] bu on o save and comple e he

se ing.

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