© 2021 SPT Labtech Ltd
6. Create Protocol ..........................................................................................49
6.1 – Aspirate ..............................................................................................................50
6.1.1 – Varieties of Menus......................................................................................50
6.1.2 – Station..........................................................................................................51
6.1.3 – Variables......................................................................................................51
6.1.4 – Pause ...........................................................................................................52
6.1.5 – Pre-Airgap and Post-Airgap .......................................................................52
6.1.6 – Increased Steps...........................................................................................52
6.1.7 – Preview Position .........................................................................................53
6.2 – Dispense.............................................................................................................54
6.2.1 – Multi-Dispensing Quadrant .......................................................................54
6.2.2 – Dispense Speed Over 60uL........................................................................54
6.2.3 – Dispense Location in Well..........................................................................54
6.2.4 – Side Touch...................................................................................................54
6.2.5 – Bottom Touch .............................................................................................55
6.2.6 – Decremental Steps .....................................................................................55
6.3 – Empty .................................................................................................................56
6.4 – Mix ......................................................................................................................57
6.4.1 Volume Retained ...........................................................................................57
6.4.2 Mix at Corners ...............................................................................................57
6.5 – Transfer..............................................................................................................58
6.6 – Serial Dilution
.................................................................................................60
6.6.1 – Start Column and End Column..................................................................60
6.6.2 – Interval.........................................................................................................60
6.6.3 – Preview Position .........................................................................................60
6.7 – Fill Plate ..............................................................................................................62
6.7.1 – Maximum Aspirate Volume .......................................................................62
6.7.2 – Remain Volume...........................................................................................62
6.7.3 – Preview Position .........................................................................................63
6.8 – Pooling................................................................................................................64
6.9 – Wash...................................................................................................................65
6.9.1 – Using Pump.................................................................................................66
6.9.2 – Pre-Fill Period..............................................................................................66
6.9.3 – Post Wash Period........................................................................................66
6.10 – Pause................................................................................................................67
