SYSMEX SP-50 Manuale utente

Sysmex House, Garamonde Drive, Wymbush, Milton Keynes, MK8 8DF, United Kingdom · Phone +44 333 320 3460 · Fax +44 1908 267 901

academy.training@sysmex.co.uk· www.sysmex.co.uk

Routine Use Training Workbook

SP-50

Date:

26/10/2020

Document Ref:

SUKBMS-24-334

Version:

4.0

Classification:

Unrestricted

IFU version:

1801

Disclaimer –SP-50 
Sysmex House, Garamonde Drive, Wymbush, Milton Keynes, MK8 8DF, United Kingdom · Phone +44 333 320 3460 · Fax +44 1908 267 901 
academy.training@sysmex.co.uk· www.sysmex.co.uk 
2 
Contents 
Contents 2 
Disclaimer 4 
Revision History 4 
Reference Documents 4 
SP-50 Overview 5 
Analyser components 6 
Outside the analyser 6 
Front Components 8 
Smear Unit 9 
Stain Unit 10 
Reagents On-board the SP-50 11 
Smear Production 12 
Staining Principles 13 
SP-50 –IPU Layout 14 
Main Screen 14 
Status Area 15 
Instrument Status 15 
Indicator 16 
Slide Preparation Mode 16 
Sample Number 16 
Sampler/Manual 17 
Mode 17 
Quick Guide: Maintenance 18 
Daily Maintenance 19 
Performing Shutdown 1 19 
Wipe Stain Baths 21 
Performing Start-up 22 
Weekly Maintenance 24 
Cleaning the Spreader Glass 24 
Cleaning the Smear/Stain Unit 27 
Cleaning the Stain Pool 30 
Monthly Maintenance 31 
Performing Shutdown 2 31 
As Required Maintenance 34 
Loading Glass Slides 34 
Replace Spreader Glass 35 

Disclaimer –SP-50 
Sysmex House, Garamonde Drive, Wymbush, Milton Keynes, MK8 8DF, United Kingdom · Phone +44 333 320 3460 · Fax +44 1908 267 901 
academy.training@sysmex.co.uk· www.sysmex.co.uk 
3 
Replacing Ink Ribbon 39 
Replacing Reagents –CellPack DCL, Stains & Buffer Solution 42 
Replacing Methanol 46 
Quick Guide: Running Samples 47 
Sample Preparation 47 
Sampler Preparation 47 
Manual Preparation 47 
Sample Volumes 48 
Sampler Mode 49 
Smearing Mode 50 
Staining & Smearing Mode 50 
Operation of the SP-50 on the XN Track 51 
Placing into ‘Offline’ or Isolation 51 
Running Samples in ‘Offline’ or Sampler Mode 51 
Manual Mode 52 
Manual –Smearing & Staining 52 
Manual –Stain Only 56 
Manual –Smear Only 59 
Manual –Print Only 62 
Responding to Alarms 65 
Audit Logs 66 
Contact Us 69 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 

Disclaimer –SP-50

Sysmex House, Garamonde Drive, Wymbush, Milton Keynes, MK8 8DF, United Kingdom · Phone +44 333 320 3460 · Fax +44 1908 267 901

academy.training@sysmex.co.uk· www.sysmex.co.uk

Disclaimer

Please note the information in this presentation, workbook or training session provided by Sysmex

should not be used as an alternative to your sites Standard Operating Procedure (SOP)/Contract. If you

have any particular questions regarding any site specific use of reagents, consumables and/or

equipment please contact your Management Team.

Revision History

Revised section

Alteration

Name

Date

Daily maintenance

Added wipe stain bath

K. Elgerton

January 2019

Weekly maintenance

Amended weekly schedule

K. Elgerton

January 2019

Reagents

Amended changing stain

K. Elgerton

January 2019

Audit logs

Added exporting CSV

K. Elgerton

January 2019

Reagents

Amended changing Buffer

K. Elgerton

January 2020

Front cover

New logo added

K. Elgerton

January 2020

Contacts

Reagent hotline updated

K. Elgerton

January 2020

Maintenance

Shutdown times updated

K. Elgerton

January 2020

Title page

Workbook name changed to

include routine use.

N. Bowen

October 2020

Reference Documents

Document title

Version

Date

SP-50_BO (IFU)

1801

January 2018

SP-50_GI (IFU)

1801

January 2018

SP-50_TS (IFU)

1801

January 2018

Reference Documents –SP-50 Overview

Sysmex House, Garamonde Drive, Wymbush, Milton Keynes, MK8 8DF, United Kingdom · Phone +44 333 320 3460 · Fax +44 1908 267 901

academy.training@sysmex.co.uk· www.sysmex.co.uk

SP-50 Overview

Facts and Figures

SP-Series analysers

SP-1000i

SP-10

SP-50

Throughput

SP-50 S –30 samples/hr

SP-50 H –75 samples/hr

Capacity

Standalone analyser –50 samples

Tracked analysers –racks are automatically sent to the SP if a

smear is requested.

Sample Type

Whole blood

Sampler Mode

Regular tube

Raised bottom tube (RBT) –requires RBT rack

Manual Mode

Manual (Closed)

Manual (Open)

Manual (RBT)

Manual (Micro)

Aspiration Volumes

Sampler mode - 70µl

Manual (Closed) mode - 70 µl

Manual (Micro) mode - 38 µl

Staining Protocols

May-Grunwald-Giemsa stain

Wright single stain

Wright-Giemsa stain

Analyser components –SP-50 Overview

Sysmex House, Garamonde Drive, Wymbush, Milton Keynes, MK8 8DF, United Kingdom · Phone +44 333 320 3460 · Fax +44 1908 267 901

academy.training@sysmex.co.uk· www.sysmex.co.uk

Analyser components

Outside the analyser

1. Main Power Switch Cover –Open this cover to turn the main power switch ON/OFF, and to

regulate the pressures if required.

2. Stain Unit Cover –Open thiscover to inspect the interior of the stain unit. Accesscan be gained

for cleaning or maintenance tasks. Locked during operation.

3. Start-up (Power) Switch –Press to start up the instrument.

4. Eject Switch –Press to eject magazines from the stain unit.

5. IPU Touchscreen –Use to operate the instrument and configure settings.

6. Sample Tube Holder –Used to set samples for manual analysis.

7. Mode Switch –Press to switch between manual analysis and sampler analysis. Pressing it

opens and closes the sample holder.

•When the sample holder is open: Manual preparation

•When the sample holder is closed: Sampler preparation

Analyser components –SP-50 Overview

Sysmex House, Garamonde Drive, Wymbush, Milton Keynes, MK8 8DF, United Kingdom · Phone +44 333 320 3460 · Fax +44 1908 267 901

academy.training@sysmex.co.uk· www.sysmex.co.uk

8. Start Switch –Press to start manual analysis.

9. Status Indicator LED - Shows the current status of the analyser.

LED Indicator Light

Description

Green

Ready (Analysis or maintenance is possible)

Flashing Green

Starting up / Analysis in progress / Maintenance in progress

Orange

Sample analysis stopped / not possible. An error has occurred, but the

instrument is still operational.

Flashing Orange

Starting up/Preparing a slide/Switching the slide preparation mode/ Shutting

down/Sleeping

Red

Stopped - Error (without alarm) / initialising system / Analyser ready to be

turned off at the end of shutdown

Flashing Red

Stopped - Error(with alarm)

Not Lit

Powered OFF

10. Manual Magazine Holders - This is used for loading manual slides into the analyser for manual

staining.

11. Lower Front Cover - Open this cover to inspect the interior of the main unit to perform cleaning

or maintenance tasks. Analyser must be turned off.

12. Manual Magazine Holder Status LED –Shows the current status of the manual magazine

holder. A magazine cannot be loaded or removed while the LED is flashing.

LED Indicator Light

Description

Green

Containing magazine

Red

Error

Not Lit

Powered OFF

Analyser components –SP-50 Overview

Sysmex House, Garamonde Drive, Wymbush, Milton Keynes, MK8 8DF, United Kingdom · Phone +44 333 320 3460 · Fax +44 1908 267 901

academy.training@sysmex.co.uk· www.sysmex.co.uk

13. Slide Unit Cover - Open to load glass slides. Locked during operation.

14. Smear Unit Cover - Open this cover to inspect the interior of the smear unit to perform cleaning

or maintenance tasks. The smear unit cover and the slide set cover are one piece. To open the

smear unit cover, remove the slide supply cassette first. Locked during operation

Front Components

1. 0.07 MPa Regulator –Regulates the source pressure to 0.07 MPa

2. Main Power Switch –Press to turn the main power of the main unit on/off

3. Slide Supply Cassette Status LED –Shows the current status of the supply cassettes. A

cassette cannot be loaded or removed while the LED is flashing.

4. Printer –Prints sample information and patient demographics onto glass slide.

5. Slide Supply Cassettes –Loads/stores glass slides for use on the SP-50.

LED Indicator Light

Description

Green

Slides loaded

Red

No slides/error

Not Lit

Powered OFF

Analyser components –SP-50 Overview

Sysmex House, Garamonde Drive, Wymbush, Milton Keynes, MK8 8DF, United Kingdom · Phone +44 333 320 3460 · Fax +44 1908 267 901

academy.training@sysmex.co.uk· www.sysmex.co.uk

Smear Unit

1. Smear Unit –Spreads the dispensed sample onto glass slide. Contains spreader glass unit.

2. Print Unit –Prints sample information and patient demographics onto glass slide. Contains the

ink ribbon.

3. Smear Dryer Unit –Dries the smeared sample.

4. Slide Supply Unit –Takes out glass slides from the supply cassettes.

Analyser components –SP-50 Overview

Sysmex House, Garamonde Drive, Wymbush, Milton Keynes, MK8 8DF, United Kingdom · Phone +44 333 320 3460 · Fax +44 1908 267 901

academy.training@sysmex.co.uk· www.sysmex.co.uk

Stain Unit

A. Stain Unit Cover –Open thiscover to inspect the interior of the stain unit. Accesscan be gained

for cleaning or maintenance tasks. Locked during operation.

B. Stain Unit –Depending upon protocol; fixes, stains, rinses and dries the prepared slides.

There are 7 staining pools which are filled up with the following reagents. The types of reagent varies

depending on the staining method. Samples move through the staining pool in the direction of the arrow

as illustrated above.

Stain Pool

Role

Reagent

Fixing

Methanol

Fixing

Stain 1

Staining

Stain 1/buffer

Rinsing

Rinse

Staining

Stain 2/buffer

Rinsing

Rinse & dry

*Use depends upon protocol

Indice

Altri manuali SYSMEX Attrezzature da laboratorio

SYSMEX

SYSMEX ogt Cytocell Multiprobe CLL Manuale utente

SYSMEX

SYSMEX SP Series Guida all'installazione

SYSMEX

SYSMEX BEYOND A BETTER BOX RU-21 Manuale utente

SYSMEX

SYSMEX CyFlow Cube 8 Manuale utente

SYSMEX

SYSMEX CS-5100 LAS Manuale utente

SYSMEX

SYSMEX XE-5000 Manuale utente

SYSMEX

SYSMEX Ploidy Manuale utente

SYSMEX

SYSMEX KX-21 Manuale utente

SYSMEX

SYSMEX ogt Cytocell Multiprobe OctoChrome Manuale utente

SYSMEX

SYSMEX CA-500 Manuale utente

Manuali Attrezzature da laboratorio popolari di altre marche

Qiagen

Qiagen DML 3000 Manuale utente

Queensgate

Queensgate NANOSCAN OP400 Manuale di programmazione

Conviron

Conviron GEN1000-GE Manuale utente

Parr Instrument

Parr Instrument 4560 Manuale di istruzioni

IKA

IKA IC control Manuale utente

Integra

Integra DOSE IT Manuale utente

Agilent Technologies

Agilent Technologies 5800 ICP-OES Manuale utente

Endress+Hauser

Endress+Hauser Cleanfit CPA875 Manuale utente

NI PXI-5422 Manuale

Collomix

Collomix Aqix Manuale utente

SPEX SamplePrep

SPEX SamplePrep 6875 Freezer/Mill Series Manuale utente

Ocean Insight

Ocean Insight FLAME-NIR+ Manuale utente

Parker

Parker ALIGN-MG-NA Manuale utente

BD 644787 Manuale utente

DENTAURUM

DENTAURUM Compact Megaplus Manuale utente

Biuged Laboratory Instruments

Biuged Laboratory Instruments BGD 626 Manuale utente

VWR

VWR SAS Super IAQ Manuale utente

illumina

illumina MiSeqDx Manuale utente