Teledyne AutoInjector Module Istruzioni operative

Automation Modules

Installation and Operation Guide

69-5233-772

Released: July 2016

Automation Modules Installation and Operation Guide

Safety Warnings

Automation Mo dules Installat ion and Operat ion Guide

Safety Warnings

Safety Before installing, operating, or maintaining this equipment, it is

imperative that all hazards and preventive measures are fully

understood. While specific hazards may vary according to

location and application, take heed in the following general

warnings:

WARNINGWARNING

Liquids associated with this instrument may be classified

as carcinogenic, biohazard, flammable, or radioactive.

Should these liquids be used, it is highly recommended that

this application be accomplished in an isolated

environment designed for these types of materials in

accordance with federal, state, and local regulatory laws,

and in compliance with your company’s chemical/hygiene

plan in the event of a spill.

WARNINGWARNING

Avoid hazardous practices! If you use this instrument in

any way not specified in this manual, the protection

provided by the instrument may be impaired.

WARNINGWARNING

If you are using flammable solvents or chemicals with this

system, vapor concentration levels may exceed the

maximum exposure levels as recommended by OSHA

Guide 1910.1000. To reduce those levels to a safe exposure,

Teledyne Isco recommends that you place the system in a

laboratory hood designed for the purpose of ventilation.

This hood should be constructed and operated in

accordance with federal state and local regulations. In the

event of a solvent or chemical spill, your organization

should have a plan to deal with these mishaps. In all cases,

use good laboratory practices and standard safety

procedures.

Hazard Severity Levels This manual applies Hazard Severity Levels to the safety alerts.

These three levels are described in the sample alerts below.

CAUTION

Cautions identify a potential hazard, which if not avoided, may

result in minor or moderate injury. This category can also warn

you of unsafe practices, or conditions that may cause property

damage.

WARNINGWARNING

Warnings identify a potentially hazardous condition, which

if not avoided, could result in death or serious injury.

Automation Modules Installation and Operation Guide

Safety Warnings

DANGER

DANGER – limited to the most extreme situations

to identify an imminent hazard, which if not

avoided, will result in death or serious injury.

Hazard Symbols The equipment and this manual use symbols used to warn of

hazards. The symbols are explained in the table below.

Hazard Symbols

Warnings and Cautions

The exclamation point within the triangle is a

warning sign alerting you of important instructions

in the instrument’s technical reference manual.

The lightning flash and arrowhead within the trian-

gle is a warning sign alerting you of “dangerous

voltage” inside the product.

Symboles de sécurité

Ce symbole signale l’existence d’instructions

importantes relatives au produit dans ce manuel.

Ce symbole signale la présence d’un danger

d’électocution.

Warnungen und Vorsichtshinweise

Das Ausrufezeichen in Dreieck ist ein Warn-

zeichen, das Sie darauf aufmerksam macht, daß

wichtige Anleitungen zu diesem Handbuch

gehören.

Der gepfeilte Blitz im Dreieck ist ein Warnzeichen,

das Sei vor “gefährlichen Spannungen” im

Inneren des Produkts warnt.

Advertencias y Precauciones

Esta señal le advierte sobre la importancia de las

instrucciones del manual que acompañan a este

producto.

Esta señal alerta sobre la presencia de alto

voltaje en el interior del producto.

Automation Modules Installation and Operation Guide

Safety Warnings

For Additional

Information

Technical assistance for the Teledyne Isco Automation Modules

can be obtained from:

Teledyne Isco

4700 Superior St.

Lincoln NE 68504

Phone: (800) 775-2965 or (402) 464-0231

Fax: (402) 465-3001

E-mail: IscoService@teledyne.com

Automation Modules Installation and Operation Guide

Safety Warnings

Automation Modules

Table of Contents

Section 1 Introduction

1.1 Overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-1

Section 2 The AutoInjector Module

2.1 AutoInjector Module Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-1

2.2 AutoInjector Module Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-8

2.2.1 Setup . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-8

2.2.2 Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-9

Section 3 The AutoSampler Module

3.1 AutoSampler Module Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-1

3.1.1 Installing the AutoSampler Module . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-1

3.1.2 Establishing Connections . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-11

3.2 Installing the AutoSampler Module with the Column Selector Module . . . . . . . 3-19

3.3 AutoSampler Module Operation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-20

3.3.1 Setup . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-20

3.3.2 Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-21

Section 4 The Column Selector Module

4.1 Column Selector Module Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-1

4.1.1 Installing the Column Selector Module . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-1

4.1.2 Establishing Column Selector Module Connections . . . . . . . . . . . . . . . . . . 4-6

4.1.3 Mounting the AutoInjector Module to the Column Selector Module . . . . . 4-8

4.1.4 Establishing AutoInjector Module Connections . . . . . . . . . . . . . . . . . . . . . 4-16

4.2 Installing the Column Selector Module with the AutoSampler Module . . . . . . . 4-16

4.3 Column Selector Module Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-16

4.3.1 Setup . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-16

Appendix A Specifications

A.1 AutoInjector Module Specifications. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . A-1

A.2 AutoSampler Module Specifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . A-2

A.3 Column Selector Module Specifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . A-3

Automation Modules

Table of Contents

1-1

Automation Modules

Section 1 Introduction

1.1 Overview The AutoInjector Module, AutoSampler Module, and Column

Selector Module allow you to increase the productivity of the

CombiFlash®EZ Prep by automating a number of tasks involved

with high-performance liquid chromatography (HPLC). These

modules are controlled by Teledyne Isco's PeakTrak®software in

order to maintain the ease-of-use you are familiar with and

practically eliminate the need for training.

The three modules can be used separately or in conjunction with

one another for even more automated operation.

AutoInjector Module The AutoInjector Module performs automated, repetitive com-

pound injections completely unattended. This allows users to

automatically purify amounts of compound larger than what can

be done with a single separation run. As a result of having this

tedious process automated, user productivity is increased.

More information regarding the AutoInjector Module including

installation, setup, and operation can be found in Section 2.

AutoSampler Module The AutoSampler Module performs automated purifications on

multiple samples. In addition, the module doubles the fraction

collection capacity of the EZ Prep by adding two collection racks.

For more flexibility, samples can consist of multiple injections.

You can even choose between maximizing rack capacity by filling

racks completely or optimizing work flow on a shared system by

having each sample sent to the next rack. The racks utilize the

same RFID rack swapping technology of the EZ Prep, allowing

users to remove completed racks and replace them with new

racks for practically unlimited fraction collection capacity.

More information regarding the AutoSampler Module including

installation, setup, and operation can be found in Section 3.

Column Selector Module The Column Selector Module allows the EZ Prep to automati-

cally access up to four prep columns. This allows users easy

access to a variety of column sizes and chemistries. It also allows

users to dedicate columns to both high and low pH separations.

When combined with the AutoSampler Module, each sample can

be separated on the column best suited for the job.

More information regarding the Column Selector Module

including installation, setup, and operation can be found in

Section 4.

Automation Modules

Section 1 Introduction

1-2

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