Thermo Scientific Evolution Pro Manuale utente

UV Visible

Evolution Pro

Spectrophotometer

User Guide

269-350000

Revision A August 2021

WARNING Avoid an explosion or fire hazard. This instrument or accessory is not

designed for use in an explosive atmosphere.

Microsoft, Windows and Excel are either trademarks or registered trademarks of Microsoft Corporation in the

United States and/or other countries. All other trademarks are the property of Thermo Fisher Scientific Inc.

and its subsidiaries.

For technical support, please contact: www.thermofisher.com

Thermo Fisher Scientific Inc. provides this document to its customers with a product purchase to use in the

product operation. This document is copyright protected and any reproduction of the whole or any part of this

document is strictly prohibited, except with the written authorization of Thermo Fisher Scientific Inc.

The contents of this document are subject to change without notice. All technical information in this

document is for reference purposes only. System configurations and specifications in this document supersede

all previous information received by the purchaser.

Thermo Fisher Scientific Inc. makes no representations that this document is complete, accurate or

error- free and assumes no responsibility and will not be liable for any errors, omissions, damage or loss

that mightresult from any use of this document, even if the information in the document is followed

properly.

This document is not part of any sales contract between Thermo Fisher Scientific Inc. and a purchaser. This

document shall in no way govern or modify any Terms and Conditions of Sale, which Terms and Conditions of

Sale shall govern all conflicting information between the two documents.

For Research Use Only. This instrument or accessory is not a medical device and is not intended to be

usedfor the prevention, diagnosis, treatment, or cure of disease.

Thermo Scientific

Evolution Pro Spectrophotometer User Guide

DANGER

Indicates a hazardous situation which, if not avoided, will result in death or

serious injury.

WARNING

Indicates a hazardous situation which, if not avoided, could result in death or

serious injury.

CAUTION

Indicates a hazardous situation which, if not avoided, could result in minor or

moderate injury.

NOTICE

Follow instructions with this label to avoid damaging the system hardware or

losing data.

Note

Contains helpful supplementary information.

Evolution Pro Spectrophotometer

Considerations

Site Preparation and Safety

Before using the system, read the site preparation and safety manual on the documentation

media provided. Always follow the safety precautions in that manual and in this document

when using the system.

Evolution Pro Spectrophotometer User Guide Thermo Scientific

Operating Precautions

WARNING

•

Do not operate this system without following the safety precautions described in this

manual and the documentation that came with your system.

•

n'utilisez le système qu'en suivant les consignes de sécurité qui figurent dans ce

manuel et dans la documentation accompagnant le système.

•

Betreiben Sie dieses System nur unter Beachtung der Sicherheitshinweise in diesem

Handbuch und in der im Lieferumfang des Systems enthaltenen Dokumentation

•

Non utilizzare questo sistema senza seguire le norme di sicurezza descritte in questo

manuale e nella documentazione di accompagnamento del sistema.

•

No opere este sistema sin sequir las precauciones de seguridad descritas en este manual

y en la documentación proporcionada con el sistema.

Operating Precautions

The spectrophotometer contains precise optical components. Handle it carefully and follow

these precautions.

•

Do not allow moisture to leak into the instrument interior

•

Wipe off spilled chemicals immediately

•

Do not drop the instrument

•

Protect the instrument from mechanical shock

•

Protect the instrument from dust

Spectrophotometer Basics

Sample Compartment Lid

Lift the blue tab at the front of the sample

compartment to raise the entire lid.

Slide the door in the lid to the back to provide easy

access to the sample position to insert or remove

a cuvette.

Stainless steel plate to place the samples to

be tested.

Sample

compartment

Sliding

door

Stainless

steel tray

Spectrophotometer Basics

Thermo Scientific

Evolution Pro Spectrophotometer User Guide

Note

The Z-height (distance from the bottom of the cell to the center of the light beam)

for the spectrophotometer is 8.5 mm. This is true for all cuvette holder accessories. If

purchasing microcuvettes be sure to specify 8.5 mm as the Z-height for the window.

Front Cover

Remove the hold-down screw on the front panel.

Pull upwards to remove it

Align the tabs on the sides with the grooves in the

instrument cover and slide the panel into place to

replace it.

The hold-down screw is generally only required

when tubing or hoses are being brought into the

sample compartment under the flaps at the bottom

of the front cover.

Installing and Removing Accessories

Standard Cell Holder

The standard cell holder included with the

instrument accommodates 10 mm pathlength cells.

Accessories that are not electrically connected are called manual accessories. It is not necessary

to power off the instrument while installing or removing a manual accessory.

Installing a Manual Accessory

Open the sample compartment door.

Remove any accessory from the location where you plan to install the new accessory (see

the instructions below).

Pin A

Finger hole

Reference position cell

holder

Pin B

Sample position

Pin B

Toggle

Evolution Pro Spectrophotometer User Guide Thermo Scientific

Smart Accessories

Position the accessory so the two alignment holes on its baseplate fit over pins A and B in

the sample compartment.

Gently push down on the front edge of the baseplate until it snaps in place under the

toggle.

Close the sample compartment door.

For operating instructions and installation details, see the user guide for the accessory.

Removing a Manual Accessory

Use the handle or finger hole on the accessory baseplate to release the plate from the

toggle.

Lift the plate off the alignment pins and remove the accessory from the sample

compartment.

Smart Accessories

Smart Accessories include cell changers and sample holders that feature:

•

Auto recognition

•

Smart alignment

•

Serial number reporting

A connector under the accessory baseplate provides data communication and power. Smart

accessories are hot swappable. It is not necessary to power off the instrument while installing

or removing a Smart Accessory.

Reference connector

Sample connector

These accessories are supported in the sample position:

•

Smart 7-cell rotary changer

–

Also supports CVC accessory

•

Smart 8-cell linear changer

–

Also supports 8-cell Peltier accessory

•

Smart sipper

Smart Accessories

Thermo Scientific

Evolution Pro Spectrophotometer User Guide

Only the Smart 7-cell rotary changer is supported in the reference position. With this 7 x 7

configuration each cell in the sample position is measured against a unique reference cuvette

in the reference position. This configuration is useful when the different

concentrations/dilutions of matrix, such as buffer solution, are present in a single batch of

samples.

Installing a Smart Accessory

Open the sample compartment and remove any cell holder or accessory.

Insert the accessory.

Grasp the accessory by the handles and lower it into the sample compartment,

aligning the connector under the baseplate with the connector in the sample

compartment floor.

Line up the two holes at the back of the accessory with pins A and B in the sample

compartment.

Alignment hole B

Alignment hole A

Pin A

Pin B

D-connector

(female)

Press down on the front of the accessory to secure the connection.

The software displays a prompt to initialize the accessory.

Smart Accessories

Evolution Pro Spectrophotometer User Guide Thermo Scientific

Click OK to initialize the accessory.

Initialization reads information about the accessory and, for cell changers, moves it to

position 1.

Refer to the user guide for the accessory for detailed operating instructions.

Removing a Smart Accessory

Grasp the handles or the cell holder (if handles are not present) and lift the front of the

accessory to release the baseplate from the toggle.

Lift the plate off the alignment pins and remove the accessory from the sample

compartment.

The software confirms the accessory has been removed.

Routing Tubing or Cables into the Sample Compartment

The front cover of the sample compartment has two openings to allow passage of tubing or

cables into the sample compartment. These openings are covered by a layer of black foam to

exclude light. Slits cut into the foam allow you to create an opening without allowing excess

light into the sample compartment.

Slits on bottom of panel

CAUTION

•

Avoid pinch hazard. Keep hands and objects clear of the accessory during

initialization.

•

Évitez tout risque de pincement. Gardez vos mains, ainsi que tout objet, à l’écart de

l’accessoire pendant l’initialisation.

•

Quetschungen sind zu vermeiden. Hände und Objekte sind während der

Initialisierung vom Zubehör fern zu halten.

•

Evitare il rischio di pizzicamento. Tenere mani e oggetti lontano dall’accessorio

durante l’inizializzazione.

•

Evite riesgos de atrapamiento. Mantenga manos y objetos lejos del alcance del

accesorio durante la inicialización.

Smart Accessories

Thermo Scientific

Evolution Pro Spectrophotometer User Guide

Ensure that the water flows freely through the circuit (hoses are not pinched) and that the

tubes do not obstruct the light beam or movement of a cell changer accessory. If the tubing

used has insulation around it, cut the insulation off at a suitable point to allow the section of

tubing that will reside in the opening to be uninsulated.

•

For the PCCU1 accessory, allow the portion of the tubing that will be inside the sample

compartment to remain uninsulated. This will not impact performance. See photographs

below.

•

For the TPS-1500W or any similar thermostatted recirculator using insulated tubing,

replace the insulation around the tubing inside the sample compartment.

The hold-down screw at the center of the sample compartment front cover should be secured

to hold the cover firmly in place when tubing or cables are present.

Reference Cell Holder

For measurements with a reference sample, install a cuvette containing a suitable reference

solution in the reference position. The reference position can accommodate all manual cell

holders and the Smart Rotary 7-Cell Changer. It cannot accommodate the Linear 8-Cell

Changers or the Smart Sipper.

Connections on the Side of the Instrument

There are three communications cable connections on the right side panel of the instrument.

Using the Spectrophotometer

Evolution Pro Spectrophotometer User Guide Thermo Scientific

Tip

The power light flashes slowly when the spectrophotometer is starting up. When the

start-up sequence is complete the light will be steady. If the power light starts to flash

rapidly this indicates an error condition. Check INSIGHT software for any error reports.

If INSIGHT it not running or shows no error

Power the spectrophotometer OFF.

Perform basic checks and prepare to collect fault information:

Check that both sample and reference beams are clear of obstacles.

Close the sample compartment doors.

Start INSIGHT software on the attached computer.

Power the spectrophotometer ON.

Watch the progress of the startup routine.

If the error repeats, note the stage (bottom left corner of the window) where the error

occurred and contact us.

•

Trigger port – connect a contact closure trigger device here

–

Used with the rapid mix kinetics accessory

–

Used with third party remote triggering devices

•

Accessory Port – provides support for planned future accessories

•

USB-B Port – connect the computer running the instrument control software here

Using the Spectrophotometer

Connecting and Powering the Spectrophotometer

Refer to the Getting Started guide supplied with the instrument (printed copy) and also on

the same media as this user guide.

Making a Measurement

Refer to the INSIGHT software help system, included with your installation of INSIGHT

software. INSIGHT software help includes quick-start guides for each application mode.

These are available with one click from the help system home screen.

The beam direction in the sample compartment is from right to left.

The beam direction in the reference compartment is from front to back.

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