Thermo Scientific MicroCL 17 Manuale utente

IEC

Instructions for use

MICROCL17/17R 21/21R

How to use this manual

Use this manual to get acquainted with your

centrifuge and its accessories.

The manual helps you to avoid inappropriate handling.

Make sure to keep it always close to the centrifuge.

A manual that is not kept available cannot provide

protection against improper handling and thus

against damage to persons and objects.

This manual comprises chapters on

•Safety regulations

•Instrument description

•Transportation and hook-up the centrifuge

•Rotor program and accessories

•Use of the centrifuge

•Maintenance and care

•Troubleshooting

•Technical data

•Index

Please fold out

Overleaf you will find a graphic illustra-

tion of the control panel with a survey of

the most important functions

rotor turns

rpm/RCF

switch key

display

run time quick run

open lid

(only for instruments with refrigeration unit)

temperature

speed/RCF

stop

start

“set“ keys“set“ keys pre-temperature-regulation

(only for instruments with refrigeration unit)

Before switching on the centrifuge

please read this manual!

Note that “min-

” stands for “rpm” and

“x g” stands for “RCF”

Control panel

Display panels

Speed / RCF

Resting state: current value (0) or pre-selected set-point value

During run: present current value of speed or RCF

(after activation of switch key)

Circulating light-points:

rotor turns

End: „End“

Error codes: flashing display with error code

Run time

Resting/End: current value (0) / “End”, or pre-selected set

point value

(in minutes, or "hd" for permanent operation)

Time selection/run: - remaining time till “0” in minutes

Permanent operation (hd): - run time passed in seconds/ minutes

„Quick run“: - run time passed in seconds/ minutes

Temperature*

according to: current sample temperature in °C

(in temperature equilibrium)

* only for instruments with refrigeration unit

Keys

start: start of the centrifuge

stop: manually stop of a run

open lid: open lid

(possible only with the instrument switched on and

standstill of the rotor)

quick run: short-term run up of the centrifuge as long as key

remains pressed

Switch

Speed/RCF: switching between speed and RCF display

Pre-temp: pre-temp-function*

"set" keys: stepwise increase/decrease of set-point values

Short pressing of any of the "set" keys:

switch from current to pre-selected set-point value, signaled by

flashing display.

(Error codes (see „troubleshooting“section):

E-14: over temperature centrifuge chamber (> 50 °C)

E-22: troubled speed measurement

E-24: lid will not open

E-31: over temperature motor

E-36: over amperage or over voltage

E-46: lid was opened manually during run

E-57: imbalance

E-60: under temperature centrifuge chamber (< -20 °C)

Contents

For your safety............................................3

Safety instructions in this manual ............................3

Proper use................................................................ 4

Improper use............................................................ 4

Centrifuging hazardous materials ............................ 4

Handling the centrifuge............................................ 4

Conformity to current standards............................... 5

Description of the instrument.................... 7

Pieces delivered.......................................................7

Safety systems......................................................... 7

Properties................................................................. 8

Before use................................................... 9

Where to install the centrifuge ................................. 9

Transport and installation the centrifuge..................9

Centrifuge with refrigeration unit .......................... 9

Mains connection................................................... 10

Removing the transport protection.........................10

Accessories .............................................. 11

Rotors for the IEC MIRCOCL 17 ............................. 12

Rotors for the IEC MICROCL 21 ............................. 14

Rotors for the IEC MICROCL 17R........................... 16

Rotors for the IEC MICROCL 21R........................... 18

Adapter...................................................................20

Handling the rotor .................................... 21

Rotor lid with a spring lock .................................21

Operation without rotor lid .................................. 22

Rotor lid with screw plug ....................................23

Aerosol-tight application......................................... 24

Checking for aerosol-tightness...........................26

Operation .................................................. 27

Switching on the centrifuge....................................27

Opening the lid.......................................................27

Closing the lid.........................................................27

Installing the rotor...................................................28

Loading the rotor....................................................30

Maximum load.................................................... 30

Filling the centrifuge tubes .................................30

Placing the tubes in the rotor..............................31

Entering parameters...............................................32

Switching from speed to RCF display....................32

Selecting speed......................................................32

Entering the RCF value..........................................33

Concerning the RCF value................................. 33

Selecting the run time ............................................34

Run time selection..............................................34

Continuous operation ......................................... 34

Setting the temperature..........................................35

Contents

Pretemp..............................................................36

Starting the centrifuge............................................37

Changing the settings during the run.....................37

Stopping the centrifuge ..........................................38

Preset run time ...................................................38

Continuous operation .........................................38

Short-time centrifugation........................................38

Removing the rotor.................................................39

Audible alarm .........................................................39

Turning off the centrifuge .......................................40

WEEE Compliance:................................................40

Maintenance and care ..............................41

Maintenance to be performed by the customer .....41

Cleaning..............................................................41

Cleaning the filter unit.........................................42

Disinfection.........................................................43

Decontamination.................................................45

Autoclaving.........................................................45

The Thermo Electron Service offer........................46

Warranty conditions................................................46

Troubleshooting .......................................47

Mechanical emergency lid release.........................47

Problems you can handle yourself.........................49

Contacting Service.................................................55

Technical data...........................................57

Component parts....................................................57

The "Easycontrol" user interface............................58

Performance...........................................................59

Electrical connections.............................................61

Appendix ...................................................63

Speed / RCF diagram.............................................65

Autoclaving protocol...............................................71

Index..........................................................73

For your safety

Safety instructions in this manual

This symbol denotes potential hazards

to persons.

This symbol denotes potential damage

to the centrifuge or parts in its imme-

diate surroundings.

Warning: hot surfaces

General danger area

Before switching on the centrifuge please

read this manual!

General hints are marked with this

symbol.

IEC centrifuges are manufactured according to current

technical standards and regulations. Nonetheless,

centrifuges may pose danger to individuals and sur-

rounding if

•they are not used as designed

•they are operated by untrained personnel

•their design is improperly changed

•the safety instructions are not followed

Therefore, personnel involved with op-

eration and maintenance of the centri-

fuge must read and follow the safety

instructions.

In addition, the pertinent regulations for prevention of

accidents must be strictly followed.

This manual is an integral part of the centrifuge as-

sembly and must be kept close at hand at all times.

When damages to the power cord or at

casing are noticed the centrifuge must

be set out of operation!

For your safety

Proper use

The centrifuge is designed to separate liquid-

suspended materials having different densities and

particle size (maximum sample density is 1.2 g/cm³ at

maximum speed).

Improper use

During a run, a safety zone of 30 cm around the centri-

fuge must be maintained where neither persons nor

hazardous materials may be present.

The centrifuge may cause harm to its user or other

persons or may damage goods if safety measures are

not followed:

Centrifuging hazardous materials

•The centrifuge is neither made inert, nor is it explo-

sion-proof. Therefore never use the centrifuge in an

explosion-prone environment.

•Do not centrifuge explosive or flammable sub-

stances. The same holds for substances prone to

react violently with each other.

•Do not centrifuge toxic or radioactive substances or

pathogenic micro-organisms without suitable safety

systems.

If microbiological samples of risk group II (accord-

ing to "Laboratory Bio-safety Manual" of WHO) are

being centrifuged, aerosol-tight bio-seals have to be

used.

For materials in a higher risk group, more than one

precaution is required.

•Should toxins or pathogenic substances enter the

centrifuge or its parts, you must perform appropri-

ate procedures for disinfection (see "Maintenance

and care – Disinfection").

•Strongly corrosive substances that may cause

damage to materials and reduce the mechanical

strength of the rotor may be centrifuged only inside

protective tubes.

Handling the centrifuge

•Use only original accessories for the centrifuge.

The only exceptions are common glass or plastic

centrifuge tubes if they are approved for the rotor

speed and RCF values.

•Never use the centrifuge unless the rotor is properly

installed.

•You may use the centrifuge only with a properly

loaded rotor. You must not overload the rotor.

•Strictly follow the rules and regulations for cleaning

and disinfection.

•If the rotor or the rotor lid shows signs of corrosion

or wear, you must stop using it.

For your safety

•Never open the lid manually if the rotor is still turn-

ing.

•You may use the emergency lid release only in

case of emergency, e.g. during an interruption of

power supply

(see chapter "Troubleshooting").

•Never use the centrifuge with an opened lid.

•Never use the centrifuge if the front panel has been

partially or totally removed.

•Changes in mechanical or electrical components of

the centrifuge may only be carried out by individu-

als authorized by Thermo Electron.

Conformity to current standards

IEC centrifuges are manufactured and tested accord-

ing to the following standards and regulations

- for all voltages

•IEC 61010-1

IEC 61010-2-020

- for 120 V only

•

- for 230 V only

•

Please get acquainted with the details of the test stan-

dards from the technical data

For your safety

for your notes

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