Thermo Scientific QuantStudio 1 Guida rapida

For Research Use Only. Not for use in diagnostic procedures.

QuantStudio™1 Real-Time PCR System

INSTALLATION, USE, AND MAINTENANCE

Firmware v1.0.x

Publication Number MAN0017853

Revision A.0

Life Technologies Holdings Pte Ltd | Block 33 | Marsiling Industrial Estate Road 3 | #07-06, Singapore 739256

For descriptions of symbols on product labels or product documents, go to thermoﬁsher.com/symbols-deﬁnition.

The information in this guide is subject to change without notice.

DISCLAIMER: TO THE EXTENT ALLOWED BY LAW, THERMO FISHER SCIENTIFIC INC. AND/OR ITS AFFILIATE(S) WILL NOT BE LIABLE FOR SPECIAL,

INCIDENTAL, INDIRECT, PUNITIVE, MULTIPLE, OR CONSEQUENTIAL DAMAGES IN CONNECTION WITH OR ARISING FROM THIS DOCUMENT,

INCLUDING YOUR USE OF IT.

Revision history: Pub. No. MAN0017853 (English)

Revision Date Description

A.0 10 October 2018 New document.

Important Licensing Information: These products may be covered by one or more Limited Use Label Licenses. By use of these products, you accept

the terms and conditions of all applicable Limited Use Label Licenses.

Trademarks: All trademarks are the property of Thermo Fisher Scientiﬁc and its subsidiaries unless otherwise speciﬁed. TaqMan is a registered

trademark of Roche Molecular Systems, Inc., used under permission and license. Cy is a registered trademark of GE Healthcare. Microsoft and

Windows are trademarks of Microsoft Corporation. Pentium is a trademark of Intel Corporation. Google and Chrome are trademarks of Google, Inc.

Contents

About this guide ............................................................ 8

■CHAPTER 1 Product information ....................................... 9

Instrument hardware description ................................................. 9

Instrument overview ........................................................ 9

Parts of the instrument ..................................................... 11

Parts of the home screen ................................................... 12

Software description ........................................................... 14

Instrument, desktop, and cloud software features ............................. 14

Folders, templates, experiments, and projects ................................ 15

Third-party software ....................................................... 16

Supported options for instrument and computer connections ........................ 16

Experiment types .............................................................. 18

■CHAPTER 2 General procedures to operate the instrument ........ 20

Precautions for use ............................................................ 20

Power on the instrument ........................................................ 21

Power off the instrument ....................................................... 21

Sign out ....................................................................... 21

Load and unload a plate in the QuantStudio™1 Real-Time PCR Instrument ............ 22

Transfer, view, or manage ﬁles and results ........................................ 23

Transfer EDS ﬁles from the instrument home screen ........................... 23

View run history and delete or transfer ﬁles from the instrument ................ 23

Manage templates (EDT ﬁles) ............................................... 23

■CHAPTER 3 Conﬁgure the instrument and manage

instrument proﬁles ....................................................... 25

Initial start‑up ................................................................. 25

Installation and instrument veriﬁcation ........................................... 26

Use the instrument without signing in ............................................ 26

Create an administrator instrument proﬁle during initial start-up .................... 27

Create a new instrument proﬁle ................................................. 27

QuantStudio

™

1 Real-Time PCR System Installation, Use, and Maintenance Guide

Overview of Settings ............................................................ 28

Manage instrument settings ..................................................... 30

Manage the instrument name (Administrator only) ............................. 31

Enable Sleep Mode ........................................................ 31

Set the idling temperature for the heated cover ................................ 31

Conﬁgure the network drive ................................................. 31

OEM connection only (Administrator only) ..................................... 31

Select a Cloud region (Administrator only) .................................... 32

Set the date and time (Administrator only) .................................... 32

Conﬁgure the network ...................................................... 32

Restore factory defaults .................................................... 34

Select a language .......................................................... 34

Learn about the instrument ..................................................... 34

View instrument speciﬁcations .............................................. 34

View and export the License Agreement ...................................... 35

Manage home screen notiﬁcations ............................................... 35

Manage maintenance, service, and calibrations .................................... 35

Update instrument software (Administrator only) .............................. 35

Enable remote instrument monitoring (Administrator only) ..................... 36

View instrument statistics .................................................. 36

View instrument logs ....................................................... 36

Manage Users ................................................................. 36

Require instrument proﬁle sign-in (Administrator only) ......................... 37

Manage the Sign Out Timer (Administrator only) ............................... 37

Manage instrument proﬁles ................................................. 37

Link an instrument proﬁle with the Thermo Fisher Cloud ........................... 38

Link an existing instrument proﬁle with the Thermo Fisher Cloud ................ 38

Create a new instrument proﬁle and link with the Thermo Fisher Cloud .......... 38

■CHAPTER 4 Create and run experiments on the instrument ....... 39

Workﬂow ..................................................................... 39

Run an experiment ............................................................. 40

Create and run an experiment from a template ................................ 40

Run an experiment from a saved ﬁle ......................................... 40

Repeat the last instrument run .............................................. 41

Edit an experiment before starting a run .......................................... 41

Enter or edit template properties ............................................ 41

Edit the run method ........................................................ 43

Deﬁne, assign, and view well details ......................................... 45

Contents

QuantStudio

™

1 Real-Time PCR System Installation, Use, and Maintenance Guide

View, pause, or stop a run ....................................................... 46

View real-time data and plots on the instrument touchscreen ................... 46

Adjust the display of real-time plots on the instrument touchscreen .............. 47

Pause or stop an instrument run ............................................. 47

Lock the touchscreen during a run ........................................... 47

Transfer EDS ﬁles from the instrument home screen ............................... 48

■CHAPTER 5 Calibrate and verify instrument performance ......... 49

Calibration and veriﬁcation schedule ............................................. 49

Calibration descriptions ........................................................ 50

View the calibration status and set reminders ..................................... 51

View calibration status and set reminders in the instrument .................... 51

View calibration status and set reminders in the Cloud ......................... 51

Perform ROI/uniformity, background, and dye calibrations .......................... 52

Workﬂow: Calibration ...................................................... 52

Prepare a calibration plate .................................................. 52

Perform calibrations ....................................................... 53

View calibration images and transfer results to USB ........................... 54

Troubleshoot calibration failure ............................................. 55

Identify contamination ...................................................... 56

Create a background plate

(optional)

......................................... 56

Perform instrument veriﬁcation using RNase P plates .............................. 57

Instrument veriﬁcation description .......................................... 57

RNase P instrument veriﬁcation plate ........................................ 58

Performance speciﬁcations pass criteria ..................................... 58

Prepare an RNase P plate ................................................... 58

Perform RNase P veriﬁcation ............................................... 59

Troubleshoot veriﬁcation failure ............................................. 61

Calibrate custom dyes .......................................................... 62

Custom dyes overview ...................................................... 62

Use a dilution series to determine an optimal custom dye concentration .......... 62

Calibrate the custom dye ................................................... 65

Calibrate for a custom melt curve experiment ..................................... 68

Contents

QuantStudio

™

1 Real-Time PCR System Installation, Use, and Maintenance Guide

■CHAPTER 6 Maintain the instrument ................................. 70

Backup or restore the instrument ................................................ 70

Decontaminate the sample block ................................................. 71

Materials required ......................................................... 71

Clean the sample block ..................................................... 71

Detailed procedures for cleaning the sample block ............................. 73

Replace the instrument fuses .................................................... 73

Materials required ......................................................... 73

Replace the fuses .......................................................... 74

Prepare the instrument to store, move, or ship .................................... 74

Move the instrument ....................................................... 75

Return the instrument for service ............................................ 75

■APPENDIX A Install and connect the instrument to a network ..... 77

Workﬂow: Install and connect to a network ........................................ 77

Before you begin ............................................................... 78

Instrument and computer connections ............................................ 78

Unpack and install the instrument ............................................... 79

Power on and follow the startup wizard ........................................... 80

Connect the instrument and the computer directly or to a LAN ....................... 80

Networking ................................................................... 81

Supported options for instrument and computer connections .................... 81

Control and monitor networked instruments .................................. 82

Ethernet port overview ..................................................... 83

Firewall ports that must be open ............................................ 83

Networking guidelines and best practices ..................................... 83

■APPENDIX B Troubleshooting ......................................... 84

■APPENDIX C Instrument speciﬁcation and layout ................... 86

Conﬁgured system dimensions .................................................. 87

Instrument clearances ..................................................... 88

Electrical requirements ......................................................... 88

Environmental requirements .................................................... 89

Network requirements .......................................................... 90

Contents

QuantStudio

™

1 Real-Time PCR System Installation, Use, and Maintenance Guide

■APPENDIX D Parts and materials ..................................... 91

Kits, consumables, accessories, and reagents ..................................... 91

Consumables (96‑well, 0.2‑mL format) ............................................ 91

Accessories ................................................................... 92

General-use materials and consumables ......................................... 92

■APPENDIX E Safety ..................................................... 93

Symbols on this instrument ..................................................... 94

Conformity symbols ........................................................ 95

Safety alerts on this instrument .................................................. 96

Location of safety labels on the instrument ................................... 97

Safety information for instruments not manufactured by Thermo Fisher Scientiﬁc ..... 97

Instrument safety .............................................................. 97

General .................................................................. 97

Physical injury ............................................................ 98

Electrical safety ........................................................... 98

Cleaning and decontamination ............................................... 98

Safety and electromagnetic compatibility (EMC) standards .......................... 98

Safety compliance ......................................................... 99

EMC ...................................................................... 99

Environmental design ..................................................... 100

Chemical safety ............................................................... 100

Biological hazard safety ........................................................ 101

■Documentation and support ........................................... 102

Related documentation ........................................................ 102

Obtain information from the Help system ........................................ 102

Customer and technical support ................................................ 102

Limited product warranty ...................................................... 103

Index ..................................................................... 104

Contents

QuantStudio

™

1 Real-Time PCR System Installation, Use, and Maintenance Guide

About this guide

This guide provides information about installing, using, and maintaining the

QuantStudio™ 1 Real-Time PCR System. For information and instructions on

performing experiments on this system, refer to QuantStudio™ Design and Analysis

Desktop Software User Guide (Pub. No. MAN0010408).

QuantStudio

™

1 Real-Time PCR System Installation, Use, and Maintenance Guide

Product information

■Instrument hardware description ........................................ 9

■Software description .................................................. 14

■Supported options for instrument and computer connections .............. 16

■Experiment types ..................................................... 18

Instrument hardware description

The QuantStudio™ 1 Real-Time PCR System uses uorescence-based polymerase

chain reaction (PCR) reagents to perform the following types of experiments.

• Quantitative detection of target nucleic acid sequences (targets).

• Qualitative detection of targets.

• Qualitative analysis of the PCR product.

For more information, see “Experiment types“ on page 18.

The instrument is congured with a 96-well 0.2-mL xed block and a 3-color coupled

lter set.

The instrument can be run directly from the touchscreen to create and start

experiments. To design experiments or to analyze data, the instrument can be

integrated with the QuantStudio™ Design and Analysis Software.

An optional barcode scanner and optional wireless adapter can be ordered separately.

Instrument

overview

QuantStudio

™

1 Real-Time PCR System Installation, Use, and Maintenance Guide

Instrument ﬁlters and supported dyes

System dyes

The instrument use a coupled three-color lter set that supports the dyes shown in the

following table and gure. For more information about available spectral dye

calibration kits, contact Support.

Peak

ﬁlter Color

Filter wavelength

(nm)[1] Factory-calibrated

dyes

Example

custom dyes

Excitation Emission

x1-m1 Blue 470 ± 15 520 ± 15 FAM™,

SYBR™Green SYT09

x2-m2 Green 520 ± 10 558 ± 12 VIC™JOE™,

HEX™, TET™[2]

x4-m4 Orange 580 ± 10 623 ± 14 ROX™, JUN™Texas Red™

[1] The central wavelengths are the optimized wavelengths.

[2] The HEX™and TET™dyes from Thermo Fisher Scientific fall within the emission wavelength range of the

system, therefore they can be added and adapted for use on the instrument.

Filters

Wavelength

(nm)

123

x1-m1 x2-m2 x4-m4

Emission

Spectra

1x1-m1 — FAM™, SYBR™Green

2x2-m2 — VIC™

3x4-m4 — JUN™, ROX™, Texas Red™

Custom dyes

The instrument can run assays designed with custom dyes. Custom dyes include the

following:

• Dyes that are not supplied by Thermo Fisher Scientic.

• Dyes or formulations of dyes that are not system dyes for the instrument.

To use a custom dye on the instrument, review the following requirements.

• Calibrate the instrument for the custom dye (see “Calibrate custom dyes“ on

page 62).

• Ensure that the custom dye excites and emits within the wavelength range of the

lters (see the lter-wavelength table in “System dyes“).

• Select a custom dye that does not overlap with other dyes used in the run (see the

lter-wavelength table in “System dyes“).

Chapter 1 Product information

Instrument hardware description

QuantStudio

™

1 Real-Time PCR System Installation, Use, and Maintenance Guide

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