UVP BioDoc-It Guida
BioDoc-It™and VisiDoc-It™
Imaging Systems
Installation and User Instructions
UVP, LLC Ultra-Violet Products Ltd.
2066 W. 11th Street Unit 1, Trinity Hall Farm Estate
Upland, CA 91786 Nuffield Road, Cambridge CB4 1TG UK
Phone: (800) 452-6788 / (909) 946-3197 Phone: +44(0)1223-420022
Fax: (909) 946-3597 Fax: +44(0)1223-420561
Web Site: www.uvp.com
81-0301-01 Rev A
BioDoc-It Imaging System VisiDoc-It Imaging Systems
BioDoc-It and VisiDoc-It Imaging Systems 2
System Introduction
BioDoc-It™ Imaging System
The BioDoc-It Imaging System enables simple documentation of fluorescent and non-fluorescent gels,
membranes, blots, film plates and assays with the ability to save images to a USB storage device for later
quantitative analysis or enhancement for publication.
Images are saved in 8-bit TIFF or JPEG formats allowing the images to be read by most PC or MAC
programs. The BioDoc-It System is a cost effective solution for archiving quality images in a compact
standalone package. No computer is required.
VisiDoc-It™ Imaging System
The VisiDoc-It Imaging System is designed for researchers who already have a transilluminator in the lab.
Configurations include a compact hood or camera stand. The compact hood fits on top of UVP’s High
Performance and Benchtop Transilluminators. The VisiDoc-It System is similar to the BioDoc-It in its
functions for simple documentation of fluorescent and non-fluorescent gels, membranes, blots, film plates
and assays with the ability to save images to the USB storage device for later quantitative analysis.
System Components
BioDoc-It Components
Refer to the packing slip and pictured components for parts included with the system.
8” LCD Touch Screen
with Tilt Capability
White Light and Darkroom
Power Switches
Wide Access
Door
USB Drive
Filter Tray (located under the
camera) with EtBr Filter
Side Access Doors (on
select models)
Main S
y
stem Power Switch
View
Port
Transilluminator
FluorCam 210 Camera and
Zoom Lens
UV to White Light Converter
Plate
(
order se
p
aratel
y)
BioDoc-It and VisiDoc-It Imaging Systems 3
Internal Storage: 75GB or more
USB 2.0 ports: 1 front, 4 in the back
USB storage device capacity: 1GB (minimum)
Software: TS software
VisiDoc-It Components
Refer to the packing slip and pictured components for parts included with the system.
VisiDoc-It™ with Hood
Transilluminator (check the
packing slip to determine if
the system includes a
transilluminator)
Hood (with handles) fits over
the filter area on the
transilluminator
FluorCam 210 Camera with
Lens Kit
EtBr Filter is Mounted in the
Top of the Hood
8” LCD Touch Screen
USB Drive System Processor
BioDoc-It and VisiDoc-It Imaging Systems 4
VisiDoc-It™ with Stand
Transilluminator with UV
Blocking Cover (check the
packing slip to determine if
the system includes a
transilluminator)
FluorCam 210 Camera with
Lens Kit
EtBr Filter is Mounted under
the Lens
8” LCD Touch Screen
USB Drive System Processor
Camera Stand with X/Y
Movement
BioDoc-It and VisiDoc-It Imaging Systems 5
System Specifications
Camera
FluorCam 210 Camera with a resolution of 640 x
480 (VGA) with a USB 2.0 PC interface. All camera
settings are factory pre-set for optimum performance
when viewing gels, films, or membranes under low
light level conditions. UVP Technical Support should
be contacted before making any adjustments to the
camera settings.
Lens
The 5.7-34.2mm zoom lens is fitted with a close-up
diopter and step-up ring. The camera can then be
held in a fixed position. The diopter is for focusing
on objects at the focus length of the BioDoc-It and
VisiDoc-It system.
Processor
Internal Storage: 75GB or more
USB 2.0 ports: 1 front, 4 in the back
USB storage device capacity: 1GB (minimum)
Software: TS software
Ethidium Bromide Filter (EtBr)
The Ethidium Bromide (EtBr) Filter (50mm sq.) UV
blocking band pass interference filter blocks UV and
IR radiation emitted from the transilluminator. The
filter is placed in the filter tray below the camera
assembly. The filter allows visualization of
fluorophores from 580-630nm, targeting the EtBr
emission which peaks at 605nm. The EtBr filter can
be substituted for other specific fluorophore filters or
removed when imaging non-fluorescent media
(protein gels, colony plates, etc.) in order to produce
brighter images.
Darkroom for the BioDoc-It System
The darkroom is light tight for optimum imaging conditions. The darkroom features:
Gel viewer window
Overhead white light
Wide access doors with UV safety switch
Side doors (darkroom may or may not include side doors)
BioDoc-It and VisiDoc-It Imaging Systems 6
Hood Enclosure or Camera Stand for the VisiDoc-It System
The compact hood enclosure is manufactured of lightweight plastic for portability. Handles are
positioned on two sides of the hood to make it easy to move the hood for access to the transilluminator
surface. The camera with a zoom lens is mounted on top of the hood or camera stand.
Transilluminator
A transilluminator is included with the BioDoc-It system. For VisiDoc-It systems, transilluminators are
optional equipment. The BioDoc-It and VisiDoc-It System can accommodate UVP’s Benchtop and
FirstLight transilluminator models. The VisiDoc-It (with stand design) can also accommodate the High
Performance UV Transilluminators (25-watt) and White/UV Transilluminators (8-watt). UVP offers a
variety of transilluminators, from the benchtop models with 8-watt, single wavelength and single
intensity to multiple wavelengths and high/low intensities, or the high-end FirstLight transilluminator.
Note: For UV protection, there is a 10-minute timer installed in the BioDoc-It system. The timer shuts
off the transilluminator after 10 minutes of operation. A switch on the front of the darkroom allows the
user to reset the timer. When the darkroom door is opened, this automatically resets the timer.
LCD Touch Screen
The display is an 8-inch VGA color touch screen connected to the darkroom cabinet with tilt adjustment.
The touch screen allows the user to preview, snap, save and print images, as well as select certain
preference options, without the need of an external mouse or keyboard.
A stylus pen is included for increased pointer control for selecting software options on the touch screen.
Focus Target
The focus target fluoresces when placed on a transilluminator or when exposed to overhead UV. The
target provides sharp, fluorescent images to aid in adjusting the focus of the camera.
Removable USB Stick
The removable USB stick has 1 GB memory (minimum) included with the system which connects to the
system allowing transfer of images.
BioDoc-It and VisiDoc-It Imaging Systems 7
Printer
UV/White Converter Plate
Optional Equipment
Refer to the ordering information section of this manual for
optional equipment part numbers.
Thermal Printer
The thermal printer provides archive quality, 256 grayscale
prints, and five optional cost-effective print sizes.
Converter Plates
The UV/White Converter Plate allows imaging of non-
fluorescent stained media with an ultraviolet transilluminator.
The converter plate is specially coated to convert the 302nm
UV output to white light, rather than using a separate white
light box.
The Visi-Blue Converter Plate converts UV to 460-470nm
designed for use with blue excitation samples and SYBR
green, SYPRO orange and GFP stains.
Transilluminators
If the system does not include a transilluminator (check the packing slip), contact UVP for ordering
information or go to UVP’s web site at www.uvp.com. A large selection of transilluminators is available
from UVP. These units include compact 8-watt models to our high performance FirstLight
Transilluminator.
BioDoc-It and VisiDoc-It Imaging Systems 8
Set-up Instructions
Components
Camera with zoom lens, bracket, filter, cables
BioDoc-It darkroom or VisiDoc-It hood or stand
USB storage device, 1 GB (minimum)
Transilluminator (may be ordered separately)
WARNING: DO NOT ATTEMPT TO CONNECT ANY WIRING WHILE THE
EQUIPMENT IS CONNECTED TO ANY POWER SUPPLY
CAUTION
Do not install the system in places with high moisture, dust, or high temperature. Do not use any oil or
petroleum based cleaner for the cabinet. Use only mild soap or detergent solution for cleaning. Ensure that
the system is turned OFF during cleaning. Keep the equipment away from motors or other large magnetic
equipment apparatus.
Installing the Filter
Systems include a 50mm sq EtBr filter. The filter is pre-installed in the VisiDoc-It hood at the UVP factory.
To install the 50mm sq Ethidium Bromide (EtBr) filter in the BioDoc-It:
Carefully remove the filter from the protective
plastic case, holding the filter at the edges to
prevent fingerprints.
The filter tray is located under the camera bracket
on the right side top of the darkroom. Slide the
tray open.
Place the filter inside the filter tray so that the text
on the edge of the filter is positioned so the letters
are right side up when facing you.
Additional filters are available. Refer to Accessories for ordering information.
BioDoc-It and VisiDoc-It Imaging Systems 9
Camera Set-up
In the BioDoc-It
The camera and zoom lens are assembled at the
UVP factory.
Remove the cap from the lens.
Attach the step up ring and diopter to the
lens. The step up ring and diopter will only
fit one way.
Using the four thumb screws provided,
secure the bracket to the base.
Slide the camera and lens assembly into the
camera bracket. Slide the lens through the
center hole in the gasket. The gasket forms
a seal around the lens.
Insert and tighten the knob/washer in the
central hole in the camera to secure the
camera to the bracket.
Camera and lens
assembl
y
Camera cable
Thumb screws
secure the bracket
to the base
Ste
p
u
p
rin
g
Dio
p
te
r
BioDoc-It and VisiDoc-It Imaging Systems 10
Plug the camera cable into the top the
camera and the other end into a UBS port on
back of the darkroom.
Connect the jumper cord from the darkroom
to the transilluminator.
Connect the main power cord from the back
of the darkroom to a surge protector power
outlet.
Connection for
jumper cord to the
transilluminator
Connection for the
darkroom power
USB port
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