UVP ColonyDoc-It Manuale utente
ColonyDoc-It™Imaging Station
Instruction Manual
UVP, LLC Ultra-Violet Products Ltd.
2066 W 11th Street, Upland, CA 91786 Unit 1, Trinity Hall Farm Estate
Tel: (800) 452-6788 / (909) 946-3197 Nuffield Road, Cambridge CB4 1TG UK
Fax: (909) 946-3597 Tel: +44(0)1223-420022 / Fax: +44(0)1223-420561
Web site: uvp.com
81-0294-01 Rev. E
ColonyDoc-It Imaging Station 2
ColonyDoc-It Imaging Station 3
END USER LICENSE AGREEMENT
PLEASE READ THE FOLLOWING AGREEMENT CAREFULLY
This Agreement is between UVP, LLC of 2066 West 11th Street, Upland, California 91786 (hereinafter “Licensor”) and the
end user of UVP software (hereinafter “Licensee”).
Licensor has developed and offers to Licensee on a non-exclusive basis pursuant to the terms and conditions set forth
hereinafter, the following software, including related copyrighted instructional materials, (collectively referred to hereafter as
“The Software”):
DOC-IT COLONY SOFTWARE
The terms of this Agreement apply without regard for the method by which the Software is acquired by Licensee. While the
most common medium for acquiring the Software from Licensor is by CD-ROM, the Software may, in some instances, be
acquired by download; from a Licensor thumb drive acquired from Licensor; from a CD ROM acquired from Licensor from a
network location; or may be pre-installed on the Licensee‟s computer.
By using the Software, you are agreeing to be bound by all terms of this License. If you do not agree to the terms of the
License, you are not authorized to use the Software in any manner.
LICENSE
In consideration of payment of the License fee, which is a portion of the price you paid, the software, including any images
incorporated in or generated by the software, and data accompanying this License and related documentation are licensed
(not sold) to you by Licensor. Licensor does not transfer title to the Software to you; this License shall not be considered a
“sale” of the software and Licensor retains full and complete title to the Software and all intellectual and industrial property
rights therein. It is to be understood that this non-exclusive and personal License only gives you the right to use and display
the software. You must treat the software like any other copyrighted material. You may not copy the Software or the written
material accompanying the software without the express written consent of Licensor.
RESTRICTIONS
The Software contains copyrighted materials, trade secrets, and other proprietary material. You may not re-sell, decompile,
reverse engineer, disassemble or otherwise reduce the Software to a human-perceivable form. Except as provided for in
this License, you may not copy, modify, network, rent, lease, or otherwise distribute the Software; nor can you make the
Software available by “bulletin boards”, on-line services, remote dial-in, or network or telecommunications links of any kind;
nor can you create derivative works or any other works that are based upon or derived from the Software in whole or in part.
You may not transfer the license rights to the Software to another party.
TERMINATION
This License is effective until terminated by either party. You may terminate this License at any time by returning the
Software to Licensor or destroying any permanent form of the software and all related documentation and all copies and
installations thereof, whether made under the terms of this License or otherwise. This License will terminate immediately
without notice from Licensor if you fail to comply with any provision of this License. Upon termination, you must destroy or
return to Licensor any permanent form of the software and related documentation.
LIMITED WARRANTY AND DISCLAIMER
Licensor warrants the software and related documentation to be free from defects in materials and workmanship, under
normal use for a period of ninety (90) days from the date of purchase as evidenced by a copy of the sales receipt or packing
slip. Licensor‟s entire liability and licensee‟s exclusive remedy will be replacement of the defective software and related
documentation or refund of the purchase price (at licensor‟s election) upon return of the software and related documentation
to licensor with a copy of proof of purchase. This warranty gives you specific legal rights and you may also have other rights
which vary from jurisdiction to jurisdiction. You expressly acknowledge and agree that use of the software is at your sole risk
after the ninety (90) days. The software and related documentation are provided without warranties and/or conditions of any
kind either express or implied, except as provided above. Licensor expressly disclaims all other warranties and/or
conditions, express or implied, with respect to software and related documentation including, but not limited to, the implied
warranties and/or conditions of merchantability and fitness for a particular purpose. Licensor does not warrant that the
functions contained in the software will be uninterrupted or error-free, or that defects in the software will be corrected after
the ninety (90) days. Furthermore, after the ninety (90) days, licensor does not warrant or make any representation
regarding the use or the results of the use of the software and related documentation in terms of their correctness, accuracy,
reliability, or otherwise. The limitations of liabilities described in this section also apply to the third party suppliers of
materials used in the software. No oral or written information or advice by licensor or by representatives of licensor shall
ColonyDoc-It Imaging Station 4
create warranties, and/or conditions, or in any way increase the scope of this limited warranty. Licensee assumes the entire
cost of all necessary servicing, repair or correction after the ninety (90) days. Some jurisdictions do not allow the exclusion
of implied warranties, so the above exclusion may not apply to you.
LIMITATION OF LIABILITY
Under no circumstances, including negligence, shall licensor be liable for any special or consequential damages that result
from the use of, or the inability to use, the software or related documentation, even if licensor or authorized representative of
licensor has been advised of the possibility of such damages. Some jurisdictions do not allow the limitation or exclusion or
liability or consequential damages, so the above limitations or exclusion may not apply to you. In no event shall licensor‟s
total liability to you for all damages, losses, and causes of action (whether in contract, tort (including negligence) or
otherwise) exceed the amount paid by you for the software.
INTENDED USE
If the equipment is used in a manner not specified by the manufacturer, the protection provided by the equipment may be
impaired.
GOVERNING LAW AND SEVERABILITY
This License shall be governed by and construed in accordance with the laws of the State of California, without giving effect
to any principles of conflicts of law. Any actions, suits or proceedings instituted in connection with this License, the Software
and/or related documentation shall be instituted and maintained exclusively in the Superior Court for the State of California,
County of Los Angeles, or in the United States District Court for the Central District of California. By entering this License
you hereby consent to the venue and jurisdiction of the aforesaid courts. If any provision of this license shall be unlawful,
void, or for any reason unenforceable, then that provision shall be deemed severable from this License and shall not affect
the validity and enforceability of any remaining provision. This is the entire agreement between the parties relating to the
subject matter herein and shall not be modified except in writing, signed by both parties.
ColonyDoc-It Imaging Station 5
Table of Contents
Introduction ............................................................................................................................................................................... 7
Components ............................................................................................................................................................................. 7
System Requirements............................................................................................................................................................... 8
System Specifications ............................................................................................................................................................... 8
Installing the ColonyDoc-It ........................................................................................................................................................ 9
Installing the Hardware ............................................................................................................................................... 9
Installing the Software .............................................................................................................................................. 11
Registering the Software .......................................................................................................................................... 11
Counting Colonies .................................................................................................................................................................. 13
Quick Capture ........................................................................................................................................................... 13
Automatic Counting .................................................................................................................................................. 15
Manual Counting ...................................................................................................................................................... 16
Creating Templates .................................................................................................................................................. 20
User Defined Template Counting ............................................................................................................................. 21
Fluorescent Colony Counting ................................................................................................................................... 22
Zone Analysis ........................................................................................................................................................... 24
Renumbering Colony/Zone Values ........................................................................................................................... 28
Spiral Plate Counting ................................................................................................................................................ 29
Editing Colonies ...................................................................................................................................................................... 31
Add Colonies ............................................................................................................................................................ 31
Delete Colonies ........................................................................................................................................................ 32
Manual Split Colonies ............................................................................................................................................... 33
Auto Split Colonies ................................................................................................................................................... 34
Merging Colonies ...................................................................................................................................................... 35
Using the Software ................................................................................................................................................................. 36
Changing Filter Settings ........................................................................................................................................... 36
Preferences .............................................................................................................................................................. 37
Toolbars ................................................................................................................................................................... 39
Reporting Functions ................................................................................................................................................................ 40
Classes ..................................................................................................................................................................... 40
Colonies ................................................................................................................................................................... 41
Statistics ................................................................................................................................................................... 41
Distribution................................................................................................................................................................ 42
Exporting to Excel ..................................................................................................................................................... 42
Supporting 21 CFR Part-11 Compliance................................................................................................................................. 44
Purpose .................................................................................................................................................................... 44
Features ................................................................................................................................................................... 44
ColonyDoc-It Imaging Station 6
Usage ....................................................................................................................................................................... 44
Servicing the ColonyDoc-It ..................................................................................................................................................... 45
Cleaning and Care .................................................................................................................................................... 45
Replacing Switches .................................................................................................................................................. 46
Replacing Fuses ....................................................................................................................................................... 46
Ordering Replacement Parts and Accessories ......................................................................................................... 46
Troubleshooting ........................................................................................................................................................ 46
Return Service Procedure ........................................................................................................................................ 47
Technical Support ..................................................................................................................................................... 48
Warranty ................................................................................................................................................................................. 48
ColonyDoc-It Imaging Station 7
Introduction
This manual covers the installation and user instructions for the ColonyDoc-It Imaging Station.
The innovative, compact design enables users to process automated, fast and accurate colony counting. The high
resolution digital color camera allows users to capture white light and fluorescent marked colonies and a wide array
of samples. The sophisticated yet intuitive system offers researchers easy detection and analysis of media.
The software loads on the user‟s computer for camera control, image capture and colony counting. The software
provides automatic and manual counting capabilities plus user defined templates for speeding up research
experiments. Users can define specific counting parameters including color differentiation and filter identification by
group or size. Once the colonies are counted, the results display on the screen. Images and data can be saved in
multiple formats and the data can be exported to Excel. The software supports 21 CFR Part 11 compliance.
Components
Colony counter
Black plate
USB cord
Power cord
Software CD
ColonyDoc-It Imaging Station 8
System Requirements
Operating System: Windows XP Professional with Service Pack-2 (32 bit only), Vista (32 bit only),
Windows 7 (32 bit only)
Internet Explorer 6.0 or higher (To determine the version of Internet Explorer, open Internet Explorer and
click on Help > About)
Minimum resolution: 1024 x 768
Intel Pentium Processor or equivalent, 1.6 GHz or higher
1GB (2GB recommended) of RAM or greater
200 MB of available hard disk space for the program, more for data
To avail the functionality of 21 CFR Part 11, then the partition must be formatted with NTFS.
CD-ROM drive
System Specifications
Electrical ratings:
Model Numbers:
97-0539-01, 97-0539-04
100-115V, 60Hz, 0.25 Amps
Model Numbers:
97-0539-02, 97-0539-05
230V, 50Hz, 0.13 Amps
Operational Ratings:
Unit intended to be used indoors
Altitude must not exceed 2000 m
Operating temperatures not to exceed 0°C to 40°C (32°F to 104°F)
Operating humidity not to exceed 85%
ColonyDoc-It Imaging Station 9
Installing the ColonyDoc-It
Installing the Hardware
Set the ColonyDoc-It Imaging Station on a
level surface.
Plug in one end of the power cord to the back
of the ColonyDoc-It Imaging Station and the
other end into a power outlet. To maintain
safety at all times, ensure that the unit is not
hard to disconnect at the location chosen.
Turn on the ColonyDoc-It by switching the I/0
switch to I located at the back of the station.
Plug one end of the USB cord to the back of
the ColonyDoc-It and the other end into a USB
port in the computer.
Removing the Doors
The ColonyDoc-It doors can be removed by
hand-loosening the two thumbnuts on the
inside of each door.
ColonyDoc-It Imaging Station 10
Installing the Filter
Note: Filters come as an optional accessory so this step is only necessary if an optical filter will be used with the
system.
The filter trays are located in the filter box attached to the ceiling of the unit. To access the trays:
Remove the four brass thumbnuts on the
filter box and pull the filter box from the
housing.
Remove the two thumbnuts that hold the filter
frame to the filter box.
Place the filter over the opening.
Replace the filter frame and thumbnuts.
Reinstall the filter box into the unit.
NOTE: It is possible to install a filter when the
filter box is installed in the unit. However,
care must be taken to avoid touching the
shiny sides of the filter.
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