V&P Scientific VP 179A Scheda tecnica

V&P Scientific, Inc. 1 Technical Note 115A

Technical Note 115A

CARE AND USE OF THE VP 179A JIG

WITH ASPIRATION MANIFOLD

Figure 1: VP 179A Jig with Aspiration Manifold

PARTS GUIDE FOR VP 179A MANIFOLD JIG

1 –Jig Slot for Shoulder Bolt

2 –Shoulder Bolt Head

3 –Manifold Hold-Down

Screw

4 –Manifold Hold-Down Bar

5 –X Positioning Screw

6 –Y Position Screw

7 –Z Bar Stop Thumb

Screw

8 –Z Arm Lever

9 –Z Arm Nylon Pressure

Screw

10 –Vacuum Outlet Fitting

11 –Vacuum Tube Support

Arm

12 –Vacuum Tubing

13 –Microplate Platform

14 –Spacer

15 –Bubble Level

V&P Scientific, Inc. 2 Technical Note 115A

SETUP FOR ASPIRATION MANIFOLD ON JIG

Attaching the Manifold

1. Loosen Manifold Hold-Down Screws (3) using the 1/16 Allen wrench and remove the

Manifold Hold Down Bars (4) (Figure 1).

2. Place the shoulder bolt heads (2) of the Manifold into the Jig slot (1) and slide down

into the slots until shoulder bolts hit the bottom of the slots.

3. Place the Manifold Hold-Down Bars (4) on top of the Manifold and tighten the

Manifold Hold-Down Screws (3) to secure the Manifold in the Jig (Figure 2a).

Setting the X and Y Positions

1. Slide a microplate into the Microplate Platform (13). Use the Allen wrench provided to

adjust the X and Y Manifold position so it is in the center of the wells of the microplate

(Figure 2a and 2b).

2. When adjusting the X Position always loosen the X Positioning Screws first in the

direction the manifold is to be moved before tightening the opposing X Positioning

Screws. Failure to do this can pull the Shoulder Bolt (2) out of the Manifold and strip

the threads.

Figure 2: Securing Manifold in Jig (a). Adjusting X (a) and Y (b) Positions.

3. For more precise positioning use one of the Registration Plates VP 903R-96, VP 903R-

384 or VP 903R-1536 (Figure 3). Place the Registration Plate into the Microplate

Platform (13) where the microplate would go and push against the back wall. Pull the

Z Arm Lever (8) down until the Manifold tubes are just over the Registration Plate.

Tighten the Z Arm Nylon Press Screw (9) to hold the Microplate Platform (13) in

position (Figure 4). Line up the white 1mm corner spots of the Registration Plate with

the corner tubes on the Manifold. This is done by using the provided 0.5 Allen wrench

to adjust the X and Y Position Screws (5 and 6) as shown in Figure 2.

V&P Scientific, Inc. 3 Technical Note 115A

Figure 3: Registration Plates

Setting the Z Height

1. Use the thumbscrew to loosen the Z Bar Stop (7) (Figure 4a).

Figure 4: (a) Adjusting Z Bar Stops. (b) Adjusting Nylon Pressure Screw for Z

Arm control.

2. Slide an empty microplate into the Microplate Platform (13). To set the plate height

where the pins stop ~1mm from the bottom of the wells, place a 1mm thick piece of

paper, or “Spacer” (14) under the microplate. Raise the Z Arm Lever (8) until the metal

tubes just touch the bottom of the wells.

VP 903R-96, -384, -1536

V&P Scientific, Inc. 4 Technical Note 115A

Figure 5: Adjusting Z height using a Spacer

3. To hold Microplate Platform (13) in this “up” position, tighten Z Arm Nylon Pressure

Screw (9).

4. Tighten the Z Bar Stop Thumb Screws (7) to lock the maximum microplate position in

place.

5. Loosen the Nylon Pressure Screw (9), lower the Microplate Platform and remove the

microplate.

6. Remove the paper spacer from the Microplate Platform and a 1mm space will be set in

the system.

Connecting the Manifold to Vacuum System

1. Connect the vacuum source to a vacuum trap (Figure 6).

Figure 6: Vacuum System Connection

V&P Scientific, Inc. 5 Technical Note 115A

2. Connect the vacuum trap to an in-line shut-off valve such as stop cock valve or a VP

600 Push Button (both are shown in Figure 6). The V&P 600 provides the ease of

turning off and on with the push of a button or by computer/robot. The stopcock

valve requires two hands to operate.

Figure 6: VP 600 Push Button In-Line Shut-Off Valve.

4. Connect the in-line shut-off valve to the Vacuum Outlet Fitting (10) with Vacuum

Tubing (12).

5. Place the Vacuum Tubing (12) in the Support Arm (11) secure to the Jig.

IMPORTANT NOTE:

The Aspiration/Dispense Manifolds are chemically resistant to some common

laboratory solvents (such as ethyl alcohol, methyl alcohol, isopropanol, DMSO)

but not all (acetone or chloroform, for example). Please contact V&P Scientific

for more information if there is any question regarding the chemical resistance

of the Manifold to the solution to be aspirated or dispensed.

VP 600

V&P Scientific, Inc. 6 Technical Note 115A

USING ASPIRATION MANIFOLDS

1. Ensure that the entire set-up is level by placing the Bubble Level (15) on the top of the

Manifold.

2. Close in-line shut-off valve. Turn on vacuum pump

3. Place the microplate to be aspirated into the Microplate Platform (13) under the

Manifold.

4. Raise the Z Arm Lever (8) to the pre-set Z Height Stop Bar (see Setting Z Height

section).

5. Open the shut-off valve to aspirate samples from wells.

6. Lower Z Arm Lever (8) and remove the microplate from the Microplate Platform (13).

7. Close in-line valve to allow pressure to build up in the vacuum trap before aspirating

from the next plate.

8. Blot tubes with VP 540DB lint free pads or rinse pins with appropriate fluid in a tray or

tip lid box before aspirating from next plate.

TROUBLESHOOTING ASPIRATION MANIFOLDS

1. If a single well not emptying properly, locate the clogged tube corresponding to the

well. Insert the “rapier” (metal wire included with the Manifold) into the clogged tube.

This should remove the obstruction. Wash the system well after performing this step.

2. Alternatively, the top portion of both the aspiration and dispense Manifolds can be

removed: the Manifold examined for clogged tubes and cleaned. Use care not to

damage the gasket when removing and replacing the top Manifold section.

STORAGE

1. For short-term storage, keep the tips of the metal aspirate tubes in the liquid in use or

distilled water. This will prevent the liquid from drying and clogging the tubes.

2. For long-term storage, drain the Manifold and aspirate three separate 100 ml distilled

water aliquots through the system. DO NOT USE DE-IONIZED WATER: De-ionized

water will corrode the stainless-steel tubes.

3. Tip the system back and forth after each aliquot to ensure all water is aspirated from

the Manifold on each rinse.

V&P Scientific, Inc. 7 Technical Note 115A

4. Aspirate two separate 50-100 ml aliquots of 100% alcohol (methanol, ethanol or

isopropyl alcohol) through the Manifold. Tip the system back and forth to ensure all

the alcohol is removed.

5. Pull air through the Manifold for 1- 2 minutes by leaving the vacuum on and shut-off

valve open.

6. Store in a clean dry area.

7. To autoclave, simply place the entire system into the autoclave. It is not necessary to

remove any parts

WARRANTY

V&P Scientific, Inc. warrants this product to be free from defects in material and

workmanship when used under normal laboratory conditions for one year. This warranty

begins from the date of delivery by V&P Scientific.

In the event this product fails under normal laboratory conditions within the specified

period of time because of a defect in material or workmanship, V&P Scientific will, at its

option, repair or replace the product. Damage to the product caused by user negligence is

not covered.

Please keep the special shipping carton in case the unit needs to be shipped back to V&P

Scientific. Please contact V&P Scientific at the address below for return authorization and

shipping instructions.

This warranty is made in lieu of other warranties expressed or implied including the

warranties of merchantability and fitness for a particular purpose. V&P Scientific shall not

be liable for loss or damages arising from the use of these products nor for consequential

damages of any kind.

If assistance is required, contact V&P Scientific, Inc. 858-455-0643 or sale[email protected]

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