WATERS CORPORATION ACQUITY Premier Manuale operativo
ACQUITY Premier TUV Detector
Overview and Maintenance Guide
715006903
Version 01
Copyright © Waters Corporation 2021
All rights reserved
General information
Copyright notice
© 2021 WATERS CORPORATION. PRINTED IN THE UNITED STATES OF AMERICA AND IN
IRELAND. ALL RIGHTS RESERVED. THIS DOCUMENT OR PARTS THEREOF MAY NOT BE
REPRODUCED IN ANY FORM WITHOUT THE WRITTEN PERMISSION OF THE PUBLISHER.
The information in this document is subject to change without notice and should not be construed
as a commitment by Waters Corporation. Waters Corporation assumes no responsibility for any
errors that may appear in this document. This document is believed to be complete and accurate
at the time of publication. In no event shall Waters Corporation be liable for incidental or
consequential damages in connection with, or arising from, its use. For the most recent revision
of this document, consult the Waters website (www.waters.com).
Trademarks
ACQUITY™ is a trademark of Waters Corporation.
ACQUITY UPLC™ is a trademark of Waters Corporation.
Empower™ is a trademark of Waters Corporation.
MassLynx™ is a trademark of Waters Corporation.
PEEK™ is a trademark of Victrex PLC.
Teflon™ is a trademark of E.I. du Pont de Nemours and Company or its affiliates.
THE SCIENCE OF WHAT'S POSSIBLE™ is a trademark of Waters Corporation.
Triton™ X-100 is a trademark of The Dow Chemical Company or an affiliated company of Dow.
UPLC™ is a trademark of Waters Corporation.
Waters™ is a trademark of Waters Corporation.
Waters Quality Parts™ is a trademark of Waters Corporation.
All other trademarks are property of their respective owners.
Customer comments
Waters’ Customer Experience and Knowledge Management organization invites you to report
any errors that you encounter in this document or to suggest ideas for otherwise improving it.
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Help us better understand what you expect from our documentation so that we can continuously
improve its accuracy and usability.
We seriously consider every customer comment we receive. You can reach us at
Contacting Waters
Contact Waters with enhancement requests or technical questions regarding the use,
transportation, removal, or disposal of any Waters product. You can reach us through the
Internet, telephone, fax, or conventional mail.
Contact method Information
www.waters.com The Waters website includes contact
information for Waters locations worldwide.
iRequest Request technical support for your Waters
instruments and software. iRequest is a free
and secure Web service that allows you to
obtain quick answers from our online
Knowledge Base, request support and service
for Waters instruments and software, or
schedule a planned service activity.
Note: In areas managed by authorized
distributors, iRequest may not be available.
Contact your local distributor for more
information.
Telephone and fax From the USA or Canada, phone
800-252-4752 or fax 508-872-1990.
For other locations worldwide, phone and fax
numbers appear in the Waters Local Offices
information.
Conventional mail Waters Corporation
Global Support Services
34 Maple Street
Milford, MA 01757
USA
Safety considerations
Some reagents and samples used with Waters instruments and devices can pose chemical,
biological, or radiological hazards (or any combination thereof). You must know the potentially
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hazardous effects of all substances you work with. Always follow Good Laboratory Practice
(GLP), and consult your organization’s standard operating procedures as well as your local
requirements for safety.
Safety hazard symbol notice
The symbol indicates a potential hazard. Consult the documentation for important
information about the hazard and the appropriate measures to prevent and control the hazard.
Considerations specific to the ACQUITY Premier TUV detector
Warning: To avoid electric shock, do not remove protective panels from the device.
The components within are not user-serviceable.
FCC radiation emissions notice
Changes or modifications not expressly approved by the party responsible for compliance, could
void the user's authority to operate the equipment. This device complies with Part 15 of the FCC
Rules. Operation is subject to the following two conditions: (1) this device may not cause harmful
interference, and (2) this device must accept any interference received, including interference
that may cause undesired operation.
Electrical power safety notice
Do not position the device so that it is difficult to disconnect the power cord.
Equipment misuse notice
If equipment is used in a manner not specified by its manufacturer, the protection provided by the
equipment may be impaired.
Safety advisories
Consult the "Safety advisories" appendix in this publication for a comprehensive list of warning
advisories and notices.
Operating the device
When operating the device, follow standard quality-control (QC) procedures and the guidelines
presented in this section.
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Applicable symbols
The following symbols can be present on the device, system, or packaging.
Symbol Definition
Manufacturer
Date of manufacture
Confirms that a manufactured product complies
with all applicable European Community
directives
UK Conformity Assessed marking confirms that
a manufactured product is in conformity with
the applicable requirements for products sold
within Great Britain
Australia EMC compliant
Confirms that a manufactured product complies
with all applicable United States and Canadian
safety requirements
Environmentally friendly use period (China
RoHS): indicates the number of years from the
date of manufacture until the product, or
components within the product, are likely to be
discarded or degrade into the environment
Consult instructions for use
Alternating current
Electrical and electronic equipment with this
symbol may contain hazardous substances and
should not be disposed of as general waste
For compliance with the Waste Electrical and
Electronic Equipment Directive (WEEE)
2012/19/EU, contact Waters Corporation for the
correct disposal and recycling instructions
For indoor use only
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Symbol Definition
No pushing
Do not connect to an LC system
10kg
max
Indicates the maximum load you can place on
that item (for example, 10kg)
Serial number
REF
Part number, catalog number
Audience and purpose
This guide is intended for individuals who install, operate, or maintain the ACQUITY Premier TUV
detector. It gives an overview of the technology and operation of the ACQUITY Premier TUV. To
install the detector, you should generally know how to set up and operate laboratory instruments
and computer-controlled devices and how to handle solvents. Use this guide in conjunction with
the system documentation and online Help.
Intended use of the detector
Waters designed the ACQUITY Premier TUV for use in liquid chromatography applications. The
ACQUITY Premier TUV is not intended for use in diagnostic applications.
Calibrating
To calibrate LC systems, adopt acceptable calibration methods using at least five standards to
generate a standard curve. The concentration range for standards must include the entire range
of QC samples, typical specimens, and atypical specimens.
When calibrating mass spectrometers, consult the instrument’s online Help system for calibration
instructions.
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Quality control
Routinely run three QC samples that represent subnormal, normal, and above-normal levels of a
compound. If sample trays are the same or very similar, vary the location of the QC samples in
the trays. Ensure that QC sample results fall within an acceptable range, and evaluate precision
from day to day and run to run. Data collected when QC samples are out of range might not be
valid. Do not report these data until you are certain that the instrument performs satisfactorily.
EMC considerations
Canada spectrum management emissions notice
This class A digital product apparatus complies with Canadian ICES-001.
Cet appareil numérique de la classe A est conforme à la norme NMB-001.
ISM classification: ISM group 1 class B
This classification was assigned in accordance with CISPR 11 Industrial Scientific and Medical
(ISM) instrument requirements.
Group 1 products apply to intentionally generated and/or used conductively coupled radio-
frequency energy that is necessary for the internal functioning of the equipment.
Class B products are suitable for use in both commercial and residential locations and can be
directly connected to a low-voltage, power-supply network.
This equipment complies with the emission and immunity requirements described in the relevant
parts of IEC/EN 61326: Electrical equipment for measurement, control, and laboratory use —
EMC requirements.
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Table of contents
General information........................................................................................................ii
Copyright notice......................................................................................................................................ii
Trademarks.............................................................................................................................................ii
Customer comments...............................................................................................................................ii
Contacting Waters..................................................................................................................................iii
Safety considerations.............................................................................................................................iii
Safety hazard symbol notice........................................................................................................... iv
Considerations specific to the ACQUITY Premier TUV detector.....................................................iv
FCC radiation emissions notice.......................................................................................................iv
Electrical power safety notice..........................................................................................................iv
Equipment misuse notice................................................................................................................ iv
Safety advisories.............................................................................................................................iv
Operating the device..............................................................................................................................iv
Applicable symbols.......................................................................................................................... v
Audience and purpose.................................................................................................................... vi
Intended use of the detector............................................................................................................vi
Calibrating....................................................................................................................................... vi
Quality control.................................................................................................................................vii
EMC considerations..............................................................................................................................vii
Canada spectrum management emissions notice..........................................................................vii
ISM classification: ISM group 1 class B..........................................................................................vii
1 Overview.....................................................................................................................12
1.1 Detector optics............................................................................................................................... 13
1.1.1 Optics assembly light path....................................................................................................13
1.1.2 Light-guiding flow cell operating principles........................................................................... 14
1.1.3 Filtering noise........................................................................................................................16
1.2 Wavelength verification and test.....................................................................................................17
1.3 Operational modes......................................................................................................................... 18
1.3.1 Single-wavelength mode.......................................................................................................18
1.3.2 Dual-wavelength mode......................................................................................................... 19
1.3.3 RatioPlot............................................................................................................................... 20
1.3.4 MaxPlot.................................................................................................................................20
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2 Preparation and operation........................................................................................ 21
2.1 Installing the detector..................................................................................................................... 21
2.2 Plumbing the detector.................................................................................................................... 23
2.3 Installing the multi-detector drip tray.............................................................................................. 26
2.4 Making Ethernet connections......................................................................................................... 28
2.5 I/O signal connector....................................................................................................................... 28
2.6 Connecting to the electricity source............................................................................................... 29
2.7 Starting the detector.......................................................................................................................30
2.7.1 Monitoring detector LEDs..................................................................................................... 31
2.7.2 About the detector control panel...........................................................................................32
2.7.3 Shutting down the detector for less than 24 hours................................................................33
2.7.4 Shutting down the detector for more than 24 hours..............................................................34
3 Maintenance procedures.......................................................................................... 35
3.1 Contacting Waters Technical Service ............................................................................................35
3.2 Safety and handling........................................................................................................................36
3.3 Spare parts.....................................................................................................................................36
3.4 Proper operating procedures..........................................................................................................36
3.5 Flushing the detector......................................................................................................................37
3.6 Maintaining the leak sensor............................................................................................................37
3.6.1 Resolving detector leak sensor errors.................................................................................. 37
3.6.2 Replacing the detector's leak sensor.................................................................................... 41
3.7 Maintaining the flow cell................................................................................................................. 44
3.7.1 Cleaning the flow cell............................................................................................................45
3.7.2 Performing a system acid-cleansing flush............................................................................ 47
3.7.3 Replacing the flow cell.......................................................................................................... 49
3.8 Replacing the lamp.........................................................................................................................52
3.9 Replacing the fuse..........................................................................................................................54
3.10 Cleaning the instrument's exterior................................................................................................55
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4 Error messages..........................................................................................................56
4.1 Startup error messages..................................................................................................................56
4.2 Error messages preventing operation............................................................................................ 58
A Safety advisories.......................................................................................................61
A.1 Warning symbols............................................................................................................................61
A.1.1 Specific warnings..................................................................................................................62
A.2 Notices...........................................................................................................................................63
A.3 Bottles Prohibited symbol.............................................................................................................. 64
A.4 Required protection........................................................................................................................64
A.5 Warnings that apply to all Waters instruments and devices...........................................................64
A.6 Warnings that address the replacement of fuses...........................................................................68
A.7 Electrical symbols.......................................................................................................................... 70
A.8 Handling symbols...........................................................................................................................71
B Specifications............................................................................................................72
B.1 Operational specifications..............................................................................................................72
B.2 Optical component specifications.................................................................................................. 73
B.3 Environmental specifications......................................................................................................... 73
B.4 Electrical specifications..................................................................................................................74
B.5 Dimensions.................................................................................................................................... 74
C Solvent considerations.............................................................................................75
C.1 Introduction....................................................................................................................................75
C.2 Solvent miscibility.......................................................................................................................... 76
C.2.1 How to use miscibility numbers............................................................................................78
C.3 Wavelength selection.....................................................................................................................78
C.3.1 UV cutoffs for common solvents...........................................................................................79
C.3.2 Mixed mobile phases............................................................................................................79
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