WATERS CORPORATION ACQUITY UPLC Manuale operativo
i
ACQUITY UPLC
TUV Detector
Operator’s Overview and Maintenance Guide
Revision A
Copyright © Waters Corporation 2010
All rights reserved
ii
Copyright notice
© 2010 WATERS CORPORATION. PRINTED IN THE UNITED STATES OF
AMERICA AND IN IRELAND. ALL RIGHTS RESERVED. THIS
DOCUMENT OR PARTS THEREOF MAY NOT BE REPRODUCED IN ANY
FORM WITHOUT THE WRITTEN PERMISSION OF THE PUBLISHER.
The information in this document is subject to change without notice and
should not be construed as a commitment by Waters Corporation. Waters
Corporation assumes no responsibility for any errors that may appear in this
document. This document is believed to be complete and accurate at the time
of publication. In no event shall Waters Corporation be liable for incidental or
consequential damages in connection with, or arising from, its use.
Trademarks
ACQUITY, ACQUITY UPLC, UPLC, Waters PIC, and Waters are registered
trademarks, and Empower, MassLynx, and “THE SCIENCE OF WHAT’S
POSSIBLE.” are trademarks of Waters Corporation.
PEEK is a trademark of Victrex Corporation.
Teflon is a registered trademark of E. I. du Pont de Nemours and Company.
TRITON is a trademark of Union Carbide Corporation.
Other registered trademarks or trademarks are the sole property of their
owners.
iii
Customer comments
Waters’ Technical Communications department invites you to tell us of any
errors you encounter in this document or to suggest ideas for otherwise
improving it. Please help us better understand what you expect from our
documentation so that we can continuously improve its accuracy and
usability.
We seriously consider every customer comment we receive. You can reach us
iv
Contacting Waters
Contact Waters®with enhancement requests or technical questions regarding
the use, transportation, removal, or disposal of any Waters product. You can
reach us via the Internet, telephone, or conventional mail.
Safety considerations
Some reagents and samples used with Waters instruments and devices can
pose chemical, biological, and radiological hazards. You must know the
potentially hazardous effects of all substances you work with. Always follow
Good Laboratory Practice, and consult your organization’s safety
representative for guidance.
When you develop methods, follow the “Protocol for the Adoption of Analytical
Methods in the Clinical Chemistry Laboratory,” American Journal of Medical
Technology, 44, 1, pages 30–37 (1978). This protocol addresses good operating
procedures and the techniques necessary to validate system and method
performance.
Waters contact information
Contacting medium Information
Internet The Waters Web site includes contact
information for Waters locations worldwide.
Visit www.waters.com.
Telephone and fax From the USA or Canada, phone 800
252-HPLC, or fax 508 872 1990.
For other locations worldwide, phone and fax
numbers appear in the Waters Web site.
Conventional mail Waters Corporation
34 Maple Street
Milford, MA 01757
USA
v
Considerations specific to the ACQUITY TUV detector
High voltage hazard
Safety advisories
Consult the Safety Advisories section on page 62 for a comprehensive list of
warning and caution advisories.
Warning: To avoid electric shock, do not remove the TUV detector’s
protective panels. The components within are not user-serviceable.
vi
Operating the ACQUITY TUV detector
When operating this instrument, follow standard quality-control (QC)
procedures and the guidelines presented in this section.
Applicable symbols
Audience and purpose
This guide is intended for personnel who install, operate, and maintain
ACQUITY TUV detectors. It gives an overview of the instrument’s technology
and operation.
Intended use of the ACQUITY TUV detector
The Waters ACQUITY TUV detector is for research use only.
Symbol Definition
Authorized representative of the European
Community
Confirms that a manufactured product complies
with all applicable European Community
directives
Australia C-Tick EMC Compliant
Confirms that a manufactured product complies
with all applicable United States and Canadian
safety requirements
This product has been tested to the requirements
of CAN/CSA-C22.2 No. 61010-1, second edition,
including Amendment 1, or a later version of the
same standard incorporating the same level of
testing requirements
Consult instructions for use
vii
Calibrating
To calibrate LC systems, follow acceptable calibration methods using at least
five standards to generate a standard curve. The concentration range for
standards must include the entire range of QC samples, typical specimens,
and atypical specimens.
When calibrating mass spectrometers, consult the calibration section of the
operator’s guide for the instrument you are calibrating. In cases where an
overview and maintenance guide, not operator’s guide, accompanies the
instrument, consult the instrument’s online Help system for calibration
instructions.
Quality-control
Routinely run three QC samples that represent subnormal, normal, and
above-normal levels of a compound. Ensure that QC sample results fall within
an acceptable range, and evaluate precision from day to day and run to run.
Data collected when QC samples are out of range might not be valid. Do not
report these data until you are certain that the instrument performs
satisfactorily.
When analyzing samples from a complex matrix such as soil, tissue,
serum/plasma, whole blood, and other sources, note that the matrix
components can adversely affect LC/MS results, enhancing or suppressing
ionization. To minimize these matrix effects, Waters recommends you adopt
the following measures:
• Prior to the instrumental analysis, use appropriate sample
pretreatment such as protein precipitation, liquid/liquid extraction
(LLE), or solid phase extraction (SPE) to remove matrix interferences.
• Whenever possible, verify method accuracy and precision using
matrix-matched calibrators and QC samples.
• Use one or more internal standard compounds, preferably isotopically
labeled analytes.
viii
Authorized representative information
Authorized representative
ISM classification
ISM Classification: ISM Group 1 Class B
This classification has been assigned in accordance with CISPR 11 Industrial
Scientific and Medical (ISM) instruments requirements. Group 1 products
apply to intentionally generated and/or used conductively coupled
radio-frequency energy that is necessary for the internal functioning of the
equipment. Class B products are suitable for use in both commercial and
residential locations and can be directly connected to a low voltage,
power-supply network.
Waters Corporation (Micromass UK Limited) is
registered in the United Kingdom with the Medicines
and Healthcare Products Regulatory Agency (MHRA)
at Market Towers, 1 Nine Elms Lane, London,
SW8 5NQ.
Waters Corporation (Micromass UK Ltd.)
Floats Road
Wythenshawe
Manchester M23 9LZ
United Kingdom
Telephone: +44-161-946-2400
Fax: +44-161-946-2480
Contact: Quality manager
Table of Contents ix
Copyright notice ................................................................................................... ii
Trademarks ............................................................................................................ ii
Customer comments ............................................................................................ iii
Contacting Waters ............................................................................................... iv
Safety considerations .......................................................................................... iv
Considerations specific to the ACQUITY TUV detector.................................... v
Safety advisories.................................................................................................. v
Operating the ACQUITY TUV detector ........................................................... vi
Applicable symbols ............................................................................................. vi
Audience and purpose......................................................................................... vi
Intended use of the ACQUITY TUV detector.................................................... vi
Calibrating ........................................................................................................ vii
Quality-control .................................................................................................. vii
Authorized representative information ...................................................... viii
Authorized representative............................................................................... viii
ISM classification .............................................................................................. viii
ISM Classification: ISM Group 1 Class B ...................................................... viii
Overview ................................................................................................................ 1
Principles of operation ....................................................................................... 2
Detector optics...................................................................................................... 2
Wavelength verification and test........................................................................ 8
Operational modes............................................................................................... 9
Before you begin ................................................................................................ 12
Installing the detector ...................................................................................... 13
Plumbing the detector ...................................................................................... 15
Installing the multi-detector drip tray ............................................................. 19
Table of Contents
x Table of Contents
Making Ethernet connections ......................................................................... 20
I/O signal connector ........................................................................................... 21
Connecting to the electricity source ............................................................. 21
Starting the detector ......................................................................................... 22
Monitoring detector LEDs................................................................................. 24
About the detector control panel....................................................................... 25
Shutting down the detector ............................................................................. 27
Shutting down for less than 24 hours............................................................... 27
Shutting down for more than 24 hours............................................................. 28
Maintaining the Detector ................................................................................. 29
Contacting Waters technical service................................................................. 29
Maintenance considerations.............................................................................. 30
Proper operating procedures ............................................................................. 30
Maintaining the leak sensor ............................................................................. 32
Replacing the detector’s leak sensor................................................................. 36
Maintaining the flow cell................................................................................... 38
Replacing the lamp ............................................................................................ 49
Replacing the fuses ............................................................................................ 52
Cleaning the instrument’s exterior................................................................... 53
Error Messages ................................................................................................... 54
Startup error messages ..................................................................................... 54
Error messages preventing operation............................................................... 58
Safety Advisories ................................................................................................ 62
Warning symbols ............................................................................................... 62
Caution symbol .................................................................................................. 65
Warnings that apply to all Waters instruments .............................................. 66
Electrical and handling symbols....................................................................... 72
Specifications ...................................................................................................... 74
Solvent Considerations .................................................................................... 77
Introduction........................................................................................................ 77
Solvent miscibility ............................................................................................. 78
Wavelength selection......................................................................................... 81
Indice
Altri manuali WATERS CORPORATION Sensore di sicurezza
