A&D PT Series Manuale utente

Pipette Accuracy Tester

PT-Series

AD-4212B-PT

AD-4212A-PT

FX-300i-PT

INSTRUCTION MANUAL

1WMPD4001794

No part of this publication may be reproduced, transmitted, transcribed, or translated

into any language in any form by any means without the written permission of A&D

Company, Limited.

The contents of this manual and the specifications of the instrument covered by this

manual are subject to change for improvement without notice.

Microsoft, Windows, Excel and Word are either registered trademarks or trademarks of

Microsoft Corporation in the United States and/or other countries.

CONTENTS

1. INTRODUCTION .............................................................................................................................2

1-1 Unpacking the Tester.......................................................................................................................... 2

1-2 Description of Each Component......................................................................................................... 4

1-3 WinCT-Pipette, Software for Pipette Accuracy Testing....................................................................... 4

1-4 Function of the Evaporation Trap ....................................................................................................... 5

2. ASSEMBLING THE TESTER..........................................................................................................6

2-1 AD-4212B-PT / AD-4212A-PT ............................................................................................................ 6

2-2 FX-300i-PT ......................................................................................................................................... 8

3. PRECAUTIONS BEFORE USE ....................................................................................................10

4. ANTI-STATIC TREATMENT ..........................................................................................................11

5. INSTALLING WinCT-Pipette ........................................................................................................11

6. CONNECTING A PC TO THE BALANCE.....................................................................................12

6-1 Connecting a PC .............................................................................................................................. 12

6-2 Checking COM Port Numbers.......................................................................................................... 13

7. CALIBRATING THE BALANCE ...................................................................................................14

7-1 AD-4212B-PT / AD-4212A-PT .......................................................................................................... 14

7-2 FX-300i-PT ....................................................................................................................................... 15

8. PREPARATION BEFORE USE.....................................................................................................16

8-1 Filling the Evaporation Trap with Water............................................................................................ 16

8-2 Preparing the Test Liquid.................................................................................................................. 16

8-3 Using the Thermometer.................................................................................................................... 17

9. MEASUREMENT ..........................................................................................................................21

9-1 Measuring Procedure ....................................................................................................................... 21

9-2 Judging the Results .......................................................................................................................... 21

9-3 Example Causes of Measurement Errors ........................................................................................22

10. AD-4212B-PT / AD-4212A-PT ....................................................................................................24

10-1 Factory Settings.............................................................................................................................. 24

10-2 Display Lock Function .................................................................................................................... 25

11. TROUBLESHOOTING ................................................................................................................26

12. SPECIFICATIONS.......................................................................................................................27

13. APPENDIX ..................................................................................................................................28

1. INTRODUCTION

The PT series Pipette Accuracy Tester performs an accuracy test of the volume of a pipette in

accordance with ISO 8655.

This manual describes how the PT series Pipette Accuracy Tester works and how to get the most out

of it in terms of performance. It includes how to assemble the tester, how to measure and how to use

the software, WinCT-Pipette, for pipette accuracy testing.

Read this manual thoroughly before using the tester and keep it at hand for future reference.

For detailed information on handling the balance, refer to the instruction manual for the balance used.

NOTE ISO 8655 is the international standard that includes pipette calibration and specifies a test

method for accuracy of the pipette volume. For detailed information, visit our web site.

1-1 Unpacking the Tester

NOTE The pipette accuracy tester is contained in a carrying case. The case is only for

carrying the tester and is not solid enough to endure transportation. So, do not

transport the tester using the carrying case. When transporting the tester, be sure to

use the original packing material.

When handling the tester, be careful not to drop it, even if it is placed in the carrying case.

The illustrations on the next page show what the PT series consists of.

Note that the USB converter is shipped in a separate package, but it can be placed in the carrying

case as shown below.

A shoulder belt and a key are provided with the carrying case. Use the shoulder belt to carry the

case.

NOTE Please confirm that the AC adapter type is correct for your local voltage and power

receptacle type.

AD-4212B-PT / AD-4212A-PT

The connection cable provided as an accessory is one meter long.

FX-300i-PT

Display

Stand

Cap A

Cap B

Evaporation trap

Weighing unit

Connection

cable (1 m)

USB converter

Carrying

case

WinCT-Pipette

(CD-ROM) Instruction manual

for the balance

AC adapter

Thermometer

clamp

Calibration

weight

(50 g)

Breeze break

Tweezers

Weighing pan unit

Test liquid cup

Sample cup

RS-232C cable

(25P-9P)

Instruction manual

for the tester

Angle

adjustment

knob

Capacity:5 mL (2 pcs)

Capacity:30 mL (2 pcs)

Sample cup holder

(30 mL)

Evaporation trap

base

Sample cup holder

(5 mL)

Shoulder

belt

Key

Balance

Fitting boss

Small breeze break

Instruction manual

for the balance

Instruction manual

for the tester

WinCT-Pipette

(CD-ROM)

Cap A

Cap B

Capacity:5 mL (2 pcs)

Capacity:30 mL (2 pcs)

Sample

cup

Tweezers

Thermometer

clamp

Calibration

weight

(100 g)

Weighing pan unit

AC adapter

USB converter

Carrying

case

RS-232C cable

(9P-9P)

Sample cup

holder (5 mL)

Sample cup

holder (30 mL)

Evaporation trap

base

Test liquid cup

Shoulder

belt

Key

1-2 Description of Each Component

zEvaporation trap

A chamber to prevent the evaporation of the dispensed test liquid.

(Refer to “1-4 Function of the Evaporation Trap”.)

zSample cup (Two types: 5 mL and 30 mL)

A cup to contain the test liquid dispensed from a pipette.

zTest liquid cup

A container to keep a test liquid such as distilled water.

zThermometer (with a clamp)

Measures the temperature of the test liquid.

zRS-232C cable and USB converter

Connects to a PC (personal computer) to transmit the test values to the PC.

zWinCT-Pipette

Software for pipette accuracy testing to convert the test liquid mass

into a volumetric value.

zCalibration weight (with a pair of tweezers to handle the weight)

A weight to calibrate the balance.

1-3 WinCT-Pipette, Software for Pipette Accuracy Testing

Features

The mass value of the distilled water dispensed from a pipette is transmitted from the balance to a

PC. Using the temperature of the test liquid and the barometric pressure that are previously entered,

the PC calculates the conversion factor (Z factor) from a mass value to a volumetric value and

converts the mass value into a volumetric value automatically, using the Z factor.

The measurement results are compared with the specifications previously entered, to perform

judgment whether the pipette will pass or fail.

Besides the pipette volume, test numbers, test dates and pipette information (manufacturer, model,

serial number) can be recorded as test data. The test data, output to a printer or stored in the PC,

allows easy management of pipette accuracy and reduces errors due to data mismanagement.

The specifications of accuracy and repeatability can be entered either as an absolute value (μL)

or as a relative value (percentage to the pipette volume).

The mass value data from the balance can be transmitted to the PC either using a COM port or

using a USB connection. (For a USB connection, use the accessory USB converter.)

For testing various pipettes easily, multiple test conditions (pipette volume, number of

measurements and specifications) can be set previously. When multiple operators are set

previously, an operator can be selected easily as necessary.

NOTE For detailed information on WinCT-Pipette, refer to “How to use WinCT-Pipette.pdf” in the

WinCT-Pipette CD-ROM.

Main Window

When WinCT-Pipette starts up, the window below opens

Specifications

Enter the pipette volume, specifications of

accuracy and repeatability used for

pass/fail judgment.

Measured Values

Displays the mass values transmitted from the

balance and the volumetric values obtained

using the Z factor.

Measurement Results

Displays the measurement results and

judgment results.

1-4 Function of the Evaporation Trap

In the pipette accuracy test using the gravimetric method, to obtain the mass of the test liquid, distilled

water is dispensed from the pipette into the sample cup installed on the balance. The obtained value,

however, is generally smaller due to evaporation loss of the test liquid. To prevent evaporation of the test

liquid, the evaporation trap is used. The ISO 8655 standard recommends that the humidity of the

measurement environment be greater than 50% RH.

Water is poured into the groove of the evaporation trap. This helps to maintain the humidity inside

the evaporation trap high and reduces evaporation. Using the evaporation trap reduces the

evaporation of the test liquid to about 25% or 0.05 mg per minute, compared with the value when the

evaporation trap is not used. This yields a measurement environment where there is no need to

consider measurement errors due to evaporation loss of the test liquid.

Another function of the evaporation trap is one as a breeze break, to prevent drafts in the

measurement environment from influencing the weighing operation and enable a stable weighing.

The evaporation trap is designed for an easy dispensing of the test liquid. The upper part has a

sloped surface so that the pipette can be inserted from obliquely above.

The Z factor is automatically calculated using the values o

the distilled water temperature and the barometric pressure.

Measurement environment

Enter the ambient relative humidity,

the distilled water temperature and

the barometric pressure.

Stand

ngle

adjustment

knob

Weighing

unit

Display

Connection

cable

Sample cup

holder (5 mL)

5 mL sample

cup

30 mL sample

cup

Sample cup

holder (30 mL)

2. ASSEMBLING THE TESTER

2-1 AD-4212B-PT / AD-4212A-PT

1. Place the weighing unit on a solid table. For details

on the installation site, refer to “3. PRECAUTIONS

BEFORE USE”.

Secure the display to the stand using the angle

adjustment knobs. Connect the display and the

weighing unit using the connection cable.

2. Install the evaporation trap base on the weighing

unit.

3. Install the sample cup holder (30 mL) on the base.

When the 30 mL sample cup is used:

Insert the sample cup into the holder.

When the 5 mL sample cup is used:

Insert the sample cup holder (5 mL) into

the sample cup holder (30 mL), and then

insert the sample cup

Evaporation

trap

base

4. Install the lower part of the evaporation trap on the

weighing unit, mating the tabs with the holes on the

base.

5. Attach the upper part of the evaporation trap to the

lower part, mating the tab with the notch.

6. Adjust the direction of the evaporation trap so that the pipette insertion opening on the sloped

surface is faced to allow easy operation.

7. Install cap A on the evaporation trap.

Remove cap B from the sloped surface.

Lower part of

the evaporation trap

Lower part of

the evaporation trap

Upper part of

the evaporation trap

右　　左

Pipette

insertion

opening

Pipette

insertion

opening

Facing to the right

of the weighing unit

Facing to the left

of the weighing unit

Cap A

Cap B

Upper part of

the evaporation trap

Tab

Hole

Tab

Notch

2-2 FX-300i-PT

1. Place the balance on a solid table.

For details on the installation site, refer to

“3. PRECAUTIONS BEFORE USE”.

2. Install the fitting boss on the balance, then install

the evaporation trap base on the fitting boss.

3. Install the sample cup holder (30 mL) on the base.

When the 30 mL sample cup is used:

Insert the sample cup into the holder.

When the 5 mL sample cup is used:

Insert the sample cup holder (5 mL) into

the sample cup holder (30 mL), and then

insert the sample cup

Evaporation

trap

base

Fitting

boss

Sample cup

holder (5 mL)

Sample cup

holder (30 mL)

5 mL sample

cup

30 mL sample

cup

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