Agilent Technologies 7100 Manuale utente

Agilent Technologies

Agilent 7100

Capillary Electrophoresis

System

User Manual

Agilent 7100 Capillary Electrophoresis System

Notices

No part of this manual may be reproduced in

any form or by any means (including elec-

tronic storage and retrieval or translation

into a foreign language) without prior agree-

ment and written consent from Agilent

Technologies, Inc. as governed by United

States and international copyright laws.

Manual Part Number

G7100-90000

Edition

12/2009

Printed in Germany

Agilent Technologies, Deutschland GmbH

Hewlett-Packard-Strasse 8

76337 Waldbronn

Warranty

The material contained in this docu-

ment is provided “as is,” and is sub-

ject to being changed, without notice,

in future editions. Further, to the max-

imum extent permitted by applicable

law, Agilent disclaims all warranties,

either express or implied, with regard

to this manual and any information

contained herein, including but not

limited to the implied warranties of

merchantability and fitness for a par-

ticular purpose. Agilent shall not be

liable for errors or for incidental or

consequential damages in connec-

tion with the furnishing, use, or per-

formance of this document or of any

information contained herein. Should

Agilent and the user have a separate

written agreement with warranty

terms covering the material in this

document that conflict with these

terms, the warranty terms in the sep-

arate agreement shall control.

Technology Licenses

The hardware and/or software described in

this document are furnished under a license

and may be used or copied only in accor-

dance with the terms of such license.

Restricted Rights Legend

If software is for use in the performance of a

U.S. Government prime contract or subcon-

tract, Software is delivered and licensed as

“Commercial computer software” as

defined in DFAR 252.227-7014 (June 1995),

or as a “commercial item” as defined in FAR

2.101(a) or as “Restricted computer soft-

ware” as defined in FAR 52.227-19 (June

1987) or any equivalent agency regulation or

contract clause. Use, duplication or disclo-

sure of Software is subject to Agilent Tech-

nologies’ standard commercial license

terms, and non-DOD Departments and

Agencies of the U.S. Government will

receive no greater than Restricted Rights as

defined in FAR 52.227-19(c)(1-2) (June

1987). U.S. Government users will receive

no greater than Limited Rights as defined in

FAR 52.227-14 (June 1987) or DFAR

252.227-7015 (b)(2) (November 1995), as

applicable in any technical data.

Safety Notices

CAUTION

A CAUTION notice denotes a haz-

ard. It calls attention to an operat-

ing procedure, practice, or the like

that, if not correctly performed or

adhered to, could result in damage

to the product or loss of important

data. Do not proceed beyond a

CAUTION notice until the indicated

conditions are fully understood and

met.

WARNING

A WARNING notice denotes a

hazard. It calls attention to an

operating procedure, practice, or

the like that, if not correctly per-

formed or adhered to, could result

in personal injury or death. Do not

proceed beyond a WARNING

notice until the indicated condi-

tions are fully understood and

met.

Research Use Only

Agilent 7100 Capillary Electrophoresis System User Manual 3

In This Guide…

This guide contains information to install and operate the

Agilent 7100 Capillary Electrophoresis System.

1 Introduction

This chapter gives an introduction to the instrument,

instrument overview and internal connectors.

2 Site Requirements and Specifications

This chapter provides information on environmental

requirements, physical and performance specifications.

3 Installing the Instrument

In this chapter you will find information about how to unpack

and to set up the Agilent CE and information about the

configuration of Instrument.

4 LAN Configuration

This chapter provides information on connecting the CE

instrument to the Agilent ChemStation PC.

5 Using the Agilent 7100 Capillary Electrophoresis System

This chapter provides information on how to set up the CE

instrument for an analysis and explains the basic settings.

6 Troubleshooting and Diagnostics

This chapter gives an overview about the troubleshooting and

diagnostic features and the different user interfaces.

7 Hardware Information

This chapter describes the instrument in more detail on

hardware and electronics.

8 Maintenance

This chapter describes the maintenance of the CE instrument.

4 Agilent 7100 Capillary Electrophoresis System User Manual

9 Parts and Materials for Maintenance

This chapter provides information on parts for maintenance.

10 Appendix

This chapter provides additional information on safety, legal

and web content associated with your 7100 CE instrument.

Agilent 7100 Capillary Electrophoresis System User Manual 5

Contents

1 Introduction 13

Introduction to the Agilent 7100 Capillary Electrophoresis System 14

Getting Help 14

Where to Find Information 15

What Learning Products are Available? 16

2 Site Requirements and Specifications 17

Site Requirements 18

Power Consideration 18

Power Cords 19

Bench Space 20

Environment 20

Physical Specifications 21

Performance Specifications 22

Anforderungen an den Aufstellungsort 23

Stromversorgung 23

Netzkabel 24

Platzbedarf 25

Umgebungsbedingungen 25

Abmessungen und Gewicht 26

Leistungsspezifikationen 27

Exigences d’installation 28

Remarques à propos de l’alimentation 28

Câbles d’alimentation 29

Encombrement 30

Environnement 30

Caractéristiques physiques 31

Caractéristiques de performance 32

6 Agilent 7100 Capillary Electrophoresis System User Manual

Contents

Requisiti ambientali 33

Alimentazione 33

Cavi di alimentazione 34

Spazio necessario 35

Ambiente 35

Specifiche fisiche 36

Specifiche delle prestazioni 37

Requisitos de las instalaciones 38

Consideraciones sobre la corriente 38

Cables de alimentación 39

Espacio en el banco 40

Entorno 40

Especificaciones físicas 41

Especificaciones de rendimiento 42

設置について 43

電源について 43

電源コード 44

作業台スペース 45

環境条件 45

物理的仕様 46

性能仕様 47

场地要求 48

电源要求 48

电源线 49

工作台位置 50

环境 50

物理规格 51

性能规格 52

3 Installing the Instrument 53

Unpacking the Agilent CE Instrument 54

Damaged Packaging 54

Unpacking the CE Instrument 54

Contents

Agilent 7100 Capillary Electrophoresis System User Manual 7

Delivery Checklist 55

Installing the Instrument 57

Physical Connection 57

4 LAN Configuration 61

What You Have to Do First 62

TCP/IP Parameter Configuration 63

Configuration Switch 64

Initialization Mode Selection 65

Link Configuration Selection 69

Storing the Settings Permanently with Bootp 70

Manual Configuration 71

With Telnet 72

Agilent Bootp Service 75

Location of the Media Access Control (MAC) Address 76

5 Using the Agilent 7100 Capillary Electrophoresis System 77

System Definition 78

Controlling the Agilent CE Instrument 79

Before You Start the Agilent CE Instrument 79

Starting Up the Agilent CE Instrument 79

Starting Your Computer and the Agilent ChemStation Software 81

Starting the Agilent ChemStation Software the First Time 82

Working with the CE Diagram 84

Common Software Toolbar 84

Software Toolbar 85

Graphical User Interface Explanation 86

Menus in the CE and DAD Diagram 88

System Vialtable 90

How to Prepare your Agilent CE Instrument for Analysis 91

Capillary Electrophoresis, CE 92

How to Use Your Agilent CE Instrument for Capillary Electrophoresis

Separations 92

8 Agilent 7100 Capillary Electrophoresis System User Manual

Contents

Agilent CE Method Parameters 92

Vials 96

Cassette Temperature 97

High Voltage System 98

Posttime and Stoptime 100

Replenishment 100

Preconditioning 101

Injection 103

Timetable 105

Detector Parameters 107

Switching the Lamp On 110

Preparing the Replenishment System if Needed 111

Preparing Vials for Buffer and Sample 115

Installing a Capillary in the Alignment Interface 124

Installing a Capillary in an Empty Capillary Cassette 128

Inserting the Cassette 132

Fraction Collection 135

Capillary Electrophoresis, Plus High Pressure CE+p 136

Requirements 136

Outlet Lift Connection to the Tubing System 138

Preparing the Agilent CE Instrument for High Pressure Use 140

Operation and Function in CE+p Mode 143

Injection Options with CE+p 146

Timetable Options with CE+p 146

CE+p Control Using the CE Diagram 147

Capillary Electrochromatography, CEC 148

How to Use Your Agilent CE Instrument for Capillary

Electrochromatography 148

Preparing the Agilent CE Instrument for High Pressure Use 150

CEC-Specific GUI Explanation 153

Injection Options with CEC 156

Timetable Options with CEC 157

CEC Control Using the CE Diagram 158

Contents

Agilent 7100 Capillary Electrophoresis System User Manual 9

Running the CEC Analysis 159

6 Troubleshooting and Diagnostics 161

CE Problem Solving 162

Power On Fail 162

Leaks 163

Blocked Replenishment Needle 165

Leakage Current 165

Broken Capillary 166

Contaminated Insulation Plate 166

Empty Injection Vial 167

Dirt in the Vials 167

Air Bubble in Injection Vial or Run Buffer Vials 167

Air Bubble in Capillary 167

Problems with the Replenishment System and Buffers

Containing SDS 168

Agilent Lab Advisor Software 169

Configuration 169

7 Hardware Information 171

Identifying Instrument Components 172

7100 Capillary Electrophoresis Instrument Electronics 173

Firmware 176

Resident System 176

Main System 176

Firmware Updates 177

Diode Array Detector 179

DAD Control 180

Digital to Analog Converter 181

Changing the Fuses for the Power Supplies 182

Installing Drainage Tubing 183

External Water Bath for Tray Cooling (Optional) 184

Analog to Digital Converter 186

10 Agilent 7100 Capillary Electrophoresis System User Manual

Contents

8 Maintenance 189

Standard Operating Procedures for Maintenance of Your

Agilent CE Instrument 190

Overview of Maintenance 191

Early Maintenance Feedback (EMF) 194

EMF Counters 194

Using the EMF Counters 194

Setting the EMF Limits 195

Cleaning the Electrodes, Pre-punchers and Insulation Plate 196

Prepare the Agilent CE Instrument 197

Remove the Covers 198

Accessing the Electrodes 199

Accessing the Liquid Handling Module 201

Accessing and Removing the Pre-punchers 203

Cleaning the Electrodes 205

Cleaning the Pre-punchers 206

Cleaning the Insulation Plate 207

Reinstalling the Pre-punchers 208

Reinstalling the Electrodes and the Insulation Plate 210

Reinstall the Capillary Cassette 215

Reinstall the Front Cover 215

Maintenance of the Replenishment System 216

Changing the Buffer Composition in the Replenishment System 217

The Replenishment System Will Not Be Used for Some Time 218

Further Action 219

Operations for Replenishment Maintenance 219

Changing the Air Inlet Filter 222

Accessing the Air Filter 222

Changing the Lamp 224

Removing the Existing Lamp 225

Inserting a New Lamp 227

Further Action 227

Cleaning the Instrument 228

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