Agilent Technologies Triple Quadrupole LC/MS Manuale utente

Agilent Triple Quadrupole LC/MS System

User Guide

Notices

Document Identification

D0037077 Revision A.00

October 2023

No part of this manual may be

reproduced in any form or by any means

(including electronic storage and retrieval

or translation into a foreign language)

without prior agreement and written

consent from Agilent Technologies, Inc.

as governed by United States and

international copyright laws.

Agilent Technologies, Inc.

5301 Stevens Creek Blvd.

Santa Clara, CA 95051

Warranty

The material contained in this document

is provided “as is,” and is subject to being

changed, without notice, in future

editions. Further, to the maximum extent

permitted by applicable law, Agilent

disclaims all warranties, either express or

implied, with regard to this manual and

any information contained herein,

including but not limited to the implied

warranties of merchantability and fitness

for a particular purpose. Agilent shall not

be liable for errors or for incidental or

consequential damages in connection

with the furnishing, use, or performance

of this document or of any information

contained herein. Should Agilent and the

user have a separate written agreement

with warranty terms covering the

material in this document that conflict

with these terms, the warranty terms in

the separate agreement shall control.

Technology Licenses

The hardware and/or software described

in this document are furnished under a

license and may be used or copied only in

accordance with the terms of such

license.

Restricted Rights Legend

U.S. Government Restricted Rights.

Software and technical data rights

granted to the federal government

include only those rights customarily

provided to end user customers. Agilent

provides this customary commercial

license in Software and technical data

pursuant to FAR 12.211 (Technical Data)

and 12.212 (Computer Software) and, for

the Department of Defense, DFARS

252.227-7015 (Technical Data -

Commercial Items) and DFARS

227.7202-3 (Rights in Commercial

Computer Software or Computer

Software Documentation).

Safety Notices

CAUTION

A CAUTION notice denotes a

hazard. It calls attention to an

operating procedure, practice, or

the like that, if not correctly

performed or adhered to, could

result in damage to the product or

loss of important data. Do not

proceed beyond a CAUTION

notice until the indicated

conditions are fully understood

and met.

WARNING

A WARNING notice denotes a

hazard. It calls attention to an

operating procedure, practice, or

the like that, if not correctly

performed or adhered to, could

result in personal injury or death.

Do not proceed beyond a

WARNING notice until the

indicated conditions are fully

understood and met.

Software Revision

This guide is valid for MassHunter 12.1,

until superseded.

Instrument Manufacturing

Manufactured by Agilent

Technologies Singapore Pte. Ltd.

No. 1 Yishun Avenue 7, Singapore

768923

Operating Temperature

Operating Temperature: 15 °C to 35 °C

Storage Temperature: -40 °C to 70 °C

Agilent Triple Quadrupole LC/MS System User Guide 3

In This Guide

This guide provides information on the Ultivo, 6475A, and 6495D LC/TQ systems

running MassHunter Data Acquisition 12.1 or higher.

Additional Resources

User Documentation

Data analysis and library management documentation can be found by scanning

the code or navigating to https://aglt.co/DALibMgmtDocs.

Instrument documentation, step by step videos, and more can be found by

scanning the code or navigating to https://aglt.co/LCMSUserDocs.

Agilent Triple Quadrupole LC/MS Supplies

Make sure that you don’t run out of essential columns and supplies. Use this quick

reference list to keep your shelves stocked by navigating to

https://aglt.co/LCTQSupplies.

4 Agilent Triple Quadrupole LC/MS System User Guide

Technical Support

To find your local sales and support contact, visit the following page:

https://www.agilent.com/en/contact-us/page

For technical support, visit the following page:

https://www.agilent.com/en/support

Agilent Community

To get answers to your questions, join over 10,000 users in the Agilent Community.

Review curated support materials organized by platform technology. Ask

questions to industry colleagues and collaborators. Get notifications on new

videos, documents, tools, and webinars relevant to your work.

https://community.agilent.com/

Agilent Triple Quadrupole LC/MS System User Guide 7

Content

Technical Support 4

1Introduction 12

Sustainability Information 13

Packaging End-of-Life 13

Product End-of-Life 13

Agilent Regulatory Compliance Statement 14

CE Compliance 14

UK Compliance 15

Electromagnetic Compatibility 16

EMC Declaration for South Korea 17

Detachable Power Cord Declaration for Japan 17

Sound Emission Certification for Federal Republic of Germany 17

Waste Electrical and Electronic Equipment (WEEE) Directive 18

2 Hardware Overview 20

Ultivo Triple Quadrupole LC/MS 21

Front view 21

Rear view 22

Calibrant Delivery System 23

6475A Triple Quadrupole LC/MS 24

Front view 24

Side view 25

Calibrant Delivery System 26

6495D Triple Quadrupole LC/MS 27

Front view 27

Side view 28

Calibrant Delivery System 29

Magnets 30

Content

Agilent Triple Quadrupole LC/MS System User Guide 8

Instrument Status Indicator Lights 33

Instrument Status Indicators in Acquisition 34

Ion Sources 35

Electrospray Ionization (ESI) source 35

Agilent Jet Stream (AJS ESI) source 36

Atmospheric Pressure Chemical Ionization (APCI) source 37

Multimode Ionization (MMI) source 38

Nanospray ESI source 39

3Tuning 40

About Tuning 41

Autotune and checktune 42

Starting the Instrument 43

Calibrating the LC/TQ (Checktune) 44

6475A and Ultivo 44

6495D 45

Configure Tune - (6495D only) 46

Calibrating the LC/TQ (Autotune) 48

6475A and Ultivo 48

6495D 49

Generating a Detailed Tune Report 50

6495D 51

Scheduling a Checktune 52

Scheduling an Autotune 54

Advanced Tune - 6495D 56

Diagnostic Tune - 6495D only 57

Content

Agilent Triple Quadrupole LC/MS System User Guide 9

4 Basic Operation 58

Overview 60

Starting Up and Shutting Down the Instrument 61

Starting in Standby mode 61

Turning off the instrument 61

Starting the Mass Hunter Data Acquisition Software 63

MassHunter Data Acquisition windows 64

Instrument Status window 65

Actuals window 65

Chromatogram Plot window 66

Spectrum window 67

Method Editor window 67

Sample Run window 68

Worklist window 68

Method Optimizer window 69

dMRM Method Split window 69

Preparing the LC Modules 70

Switch LC stream to Waste 70

Preparing the Triple Quad LC/MS 74

Performing a checktune or autotune 74

Switching LC stream to MS 74

Monitoring MS baseline and spectral displays 74

Viewing the System Activity Log for events and errors 75

Content

Agilent Triple Quadrupole LC/MS System User Guide 10

Setting Up an Acquisition Method 76

Setting Up and Running a Single Sample 80

Setting Up and Running a Worklist 81

Reviewing Results with Qualitative Analysis 85

Analyzing Data with Quantitative Analysis 86

5 Maintenance 88

Maintenance Overview 90

Routine Tasks 91

Cleaning the spray chamber - daily 91

Checking and filling the calibrant bottle - weekly 92

Cleaning the spray chamber - weekly 93

Flushing the nebulizer - weekly 96

Periodic Tasks 98

Checking the rough pump oil level 98

Draining and replacing the MS40+ pump oil and replacing the filter 99

Draining and replacing the MS120+ pump oil 101

Replacing the MS120+ pump filter 103

Replace Oil Mist/Exhaust Filter - MS120+ 104

Replacing the nitrogen gas filters 105

General Maintenance Tasks 107

Installing or removing the nebulizer 107

Inspecting the nebulizer 109

Adjusting or replacing the nebulizer needle 111

Installing the capillary 113

Removing the capillary (system with a VacShield) 115

Cleaning the capillary 116

Cleaning a clogged capillary 118

Flushing the Calibrant Delivery System 119

Replacing the electron multiplier horn (Ultivo only) 121

Replacing the MS selection valve rotor seal (Ultivo only) 122

Cleaning the corona needle 123

Content

Agilent Triple Quadrupole LC/MS System User Guide 11

Replacing the corona needle 124

Disinfection 125

Source Maintenance 126

Changing to a source that uses a different tuning mix 126

Installing and removing sources 126

Electrospray Ionization (ESI) source 128

Agilent Jet Stream (AJS ESI) source 130

Atmospheric Pressure Chemical Ionization (APCI) source 132

Multimode Ionization (MMI) source 134

Agilent Triple Quadrupole LC/MS System User Guide 12

1Introduction

Sustainability Information 13

Packaging End-of-Life 13

Product End-of-Life 13

Agilent Regulatory Compliance Statement 14

CE Compliance 14

UK Compliance 15

Electromagnetic Compatibility 16

EMC Declaration for South Korea 17

Detachable Power Cord Declaration for Japan 17

Sound Emission Certification for Federal Republic of Germany 17

Waste Electrical and Electronic Equipment (WEEE) Directive 18

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