Agilent Technologies 7250 Manuale utente

Agilent Technologies

Agilent

7250 Accurate-Mass

Quadrupole

Time-of-Flight GC/MS

System

Concepts Guide

2 Agilent 7250 Q-TOF GC/MS Concepts Guide

Notices

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tronic storage and retrieval or translation

into a foreign language) without prior agree-

ment and written consent from Agilent

Technologies, Inc. as governed by United

States and international copyright laws.

Manual Part Number

G7250-90003

Edition

First Edition, July 2017

Printed in USA

Agilent Technologies, Inc.

5301 Stevens Creek Blvd.

Santa Clara, CA 95051

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ject to being changed, without notice,

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Notices

CAUTION

A CAUTION notice denotes a haz-

ard. It calls attention to an operat-

ing procedure, practice, or the like

that, if not correctly performed or

adhered to, could result in damage

to the product or loss of important

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conditions are fully understood and

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WARNING

A WARNING notice denotes a

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proceed beyond a WARNING

notice until the indicated condi-

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met.

Microsoft® and Windows® are U.S. regis-

tered trademark s of Microsoft Corporation.

Software Revision

This guide applies to the Agilent Mass-

Hunter Workstation Software -- Data Acqui-

sition for 7250 Quadrupole Time-of-Flight

program version B.03.00 or higher until

superseded.

Agilent 7250 Q-TOF GC/MS Concepts Guide 3

In This Guide...

The Concepts Guide presents “The Big Picture” behind the

operation of the Agilent 7250 Accurate-Mass Quadrupole

Time-of-Flight GC/MS System by helping you understand how

the hardware and software work.

1 Overview

Learn how the 7250 Q-TOF GC/MS helps you do your job.

2 Theory and Inner Workings

Learn the concepts you need to understand how the 7250

Q-TOF works.

3 Agilent 7250 Accurate-Mass Q-TOF and Performance

Learn how the 7250 Q-TOF exceeds performance measures.

4 Agilent 7250 Q-TOF GC/MS Concepts Guide

Agilent 7250 Q-TOF GC/MS Concepts Guide 5

Contents

1 Overview

System Description 8

Q-TOF applications 9

Instrument control software 10

Data analysis software 12

Reference Documentation 14

2 Theory and Inner Workings

The 7250 Q-TOF Mass Spectrometer 16

Sample Ionization Technology 18

Electrion Impact Ionization 18

LE-EI Ion Source 18

Quadrupole Collision Cell MS/MS Technology 22

Quadrupole filtration 22

Quad mass filter 24

Ion fragmention and collision cell technology 26

Collision cell 26

Time-of-Flight MS Operation 30

Proprietary INVAR flight tube 31

Detector 32

Pumping system 34

3 Agilent 7250 Accurate-Mass Q-TOF Performance

How the 7250 Q-TOF Meets Performance Measures 36

Resolving Power 36

Mass Accuracy 36

Scan Speed 36

Sensitivity 37

Dynamic Range 37

6 Agilent 7250 Q-TOF GC/MS Concepts Guide

7250 Accurate-Mass Q-TOF Design 38

Agilent Technologies

Agilent 7250 Accurate-Mass Quadrupole Time-of-Flight GC/MS System

Concepts Guide

Overview

System Description 8

Q-TOF applications 9

Instrument control software 10

Data analysis software 12

This chapter provides an overview of the Agilent 7250

Accurate-Mass Quadrupole Time-of-Flight (Q-TOF) GC/MS

system, its applications, and its data analysis system.

8 Agilent 7250 Q-TOF GC/MS Concepts Guide

1 Overview

System Description

The Agilent 7250 Accurate-Mass Quadrupole Time-of-Flight

(Q-TOF) GC/MS is a stand-alone quadrupole time-of-flight mass

spectrometer for use with the Agilent 7890 Gas Chromatograph.

The 7250 Q-TOF features:

•A new independently MS-heated high efficiency, Low Energy

Electron Impact (LE-EI) ion source capable of operating in

standard (70 eV) or low energy (9 eV to 30 eV) modes

•Both 70 eV and 15 eV tune parameters are stored in the same

tune file allowing use of both modes in different time

segments for the same sample run

•Automated fast venting, that uses nitrogen to break vacuum

thereby reducing the influx of air into the analyzer, resulting

in lower stabilization times after pump down

•Independently MS-heated hyperbolic quadrupole mass filter,

which can be heated to high temperatures, minimizing the

contamination typical with low temperature analyses

•Single hexapole collision cell

•Ion-focusing slicer to improve resolution

•A new 50% longer vacuum-insulated flight tube provides

increased resolution and uses a dual-stage ion mirror for

improved mass accuracy

•A new faster pulser than on the 7200 Q-TOF that allows

extension of the mass range up to 3000 m/z

•A new 5 GS/s sampling rate with or without Transient-Level

Peak Picking (TLPP)

•Analog detector

•Independently GC-heated GC/MS interface

•Full RoHS compliance

•Three turbomolecular vacuum pumps

•Rotary vane foreline pump

•An optional isolated dry foreline pump

Overview 1

Q-TOF applications

Agilent 7250 Q-TOF GC/MS Concepts Guide 9

The 7250 Q-TOF incorporates a hexapole collision cell blanketed

with a nitrogen and helium gas mix, to improve the ion

fragmentation prior to detection quantification. It also includes

ion optics for focusing the ions prior to the flight tube and

detector.

This configuration has advantages for many applications. The

data is interpreted through the use of the MassHunter

WorkStation software, which provides quantitative and

qualitative analyses of the data obtained.

The 7250 Q-TOF can operate in either MS (TOF) mode or MS/MS

mode. When operating in MS mode, the instrument can obtain

high resolution, accurate mass measurements, and fast full

spectrum acquisition. MS/MS mode provides high resolution,

accurate mass measurements, and fast full product ion spectra

with high sensitivity. These features make the 7250 Q-TOF a

good tool for solving complex analytical problems, resolving

structural elucidation of unknowns, and confirmation of

non-target compounds.

Q-TOF applications

The 7250 Q-TOF GC/MS combination can quantify trace organic

compounds in complex matrices. The following applications use

this type of quantification:

•Food safety and research

•Environmental contaminants studies

•Toxicology

•Forensics

•Metabolomics and Metabolomics

•Natural product research

•Human and animal doping studies

•Energy research and petrochemicals

•Homeland security

Paired with the Agilent 7890 GC, the 7250 Q-TOF delivers

sensitive, reproducible analyses of target compounds in

complex matrices. This provides the following:

10 Agilent 7250 Q-TOF GC/MS Concepts Guide

1 Overview

Instrument control software

•Femtogram-level limits of detection and quantification

•Selective quantification of target compounds in high

chemical background samples

•Improved signal-to-noise ratios in complex matrices

•Ability to meet stricter regulations on sample analytical

limits for certain applications

•Simplified operation with Agilent’s data analysis software

•Fast MS/MS operation

•Reproducible mass accuracy

The 7250 Q-TOF offers the improved sensitivity in GC/MS

analysis that is required by many commercial and regulatory

applications.

Instrument control software

The MassHunter WorkStation Instrument Control software

allows you to perform the following tasks from a single window:

Prepare the instrument

•Start and stop the instrument from the software

•Download settings to the GC and the Q-TOF in real time to

control the instrument

•Evaluate if the MS parameters are within the limits to

produce the specified mass accuracy and resolution with

a Checktune report

•Optimize MS parameters automatically (Autotune) or

manually through Agilent tuning programs and print a tune

report

•Monitor the actual conditions of the instrument

•View the real-time plot for chromatograms and instrument

parameters (both GC and MS) and print a real-time plot

report

•View the centroided line spectrum of a peak or the mass

range profile spectrum of a peak in real time

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