Agilent Technologies G1992A Manuale utente

Agilent G1992A

Nanospray Ion Source

User Guide

Nanospray Ion Source User Guide

Notices

No part of this manual may be reproduced in

any form or by any means (including elec-

tronic storage and retrieval or translation

into a foreign language) without prior agree-

ment and written consent from Agilent

Technologies, Inc. as governed by United

States and international copyright laws.

Manual Part Number

G1988-90000

Edition

November, 2012

Printed in USA

Agilent Technologies, Inc.

5301 Stevens Creek Blvd.

Santa Clara, CA 95051 USA

Warranty

The material contained in this docu-

ment is provided “as is,” and is sub-

ject to being changed, without notice,

in future editions. Further, to the max-

imum extent permitted by applicable

law, Agilent disclaims all warranties,

either express or implied, with regard

to this manual and any information

contained herein, including but not

limited to the implied warranties of

merchantability and fitness for a par-

ticular purpose. Agilent shall not be

liable for errors or for incidental or

consequential damages in connec-

tion with the furnishing, use, or per-

formance of this document or of any

information contained herein. Should

Agilent and the user have a separate

written agreement with warranty

terms covering the material in this

document that conflict with these

terms, the warranty terms in the sep-

arate agreement shall control.

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The hardware and/or software described in

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Safety Notices

CAUTION

A CAUTION notice denotes a haz-

ard. It calls attention to an operat-

ing procedure, practice, or the like

that, if not correctly performed or

adhered to, could result in damage

to the product or loss of important

data. Do not proceed beyond a

CAUTION notice until the indicated

conditions are fully understood and

met.

WARNING

A WARNING notice denotes a

hazard. It calls attention to an

operating procedure, practice, or

the like that, if not correctly per-

formed or adhered to, could result

in personal injury or death. Do not

proceed beyond a WARNING

notice until the indicated condi-

tions are fully understood and

met.

Nanospray Ion Source User Guide 3

In This Guide…

This guide contains information to install and set up the

Nanospray Ion Source.

1 Installation

In this chapter you install the source and prepare the system for

analysis.

2MassReferenceSolution

This chapter describes how to use the Mass Reference Solution

to calibrate the Nanospray Ion Source.

3Safety

This chapter contains safety information.

4 Reference

This chapter contains parts lists and other reference

information.

4 Nanospray Ion Source User Guide

Nanospray Ion Source User Guide 5

Content

1 Installation 7

Upgrade Preparation 8

To remove the ESI or Agilent Jet Stream source 8

To upgrade the gas distributor 9

To upgrade the stainless steel end plate 12

To assemble and install the new spray shield and gas diverter assembly 13

Installation 16

To install the source 17

To set the position of the Counter Electrode 18

To set up MassHunter program for the Nanospray Ion Source 21

To install the power cord and camera 22

To assemble the Infusion Sample Delivery System 25

To infuse solution through the Infusion Sample Delivery System 26

To assemble the Extension Tube column support assembly for LC analysis 27

To install the Needle Sleeve Assembly into the Nanospray Ion Source 32

To install the Nanospray Slide Assembly for inline analysis 36

To adjust the LED camera light 41

2MassReferenceSolution43

To prepare the Low Mass IRM stock solution 45

To prepare the IRM working solution 46

To apply the IRM solutions 47

To enable reference mass correction 50

To clean the wick and wick cover 52

3Safety53

Safety Issues 54

Safety Symbols 58

6 Nanospray Ion Source User Guide

Contents

4 Reference 59

Parts List 60

Component Alignment 67

Hole Layout 68

Agilent G1992A Nanospray Ion Source

User Guide

Installation

Upgrade Preparation 8

To remove the ESI or Agilent Jet Stream source 8

To upgrade the gas distributor 9

To upgrade the stainless steel end plate 12

To assemble and install the new spray shield and gas diverter

assembly 13

Installation 16

To install the source 17

To set the position of the Counter Electrode 18

To set up MassHunter program for the Nanospray Ion Source 21

To install the power cord and camera 22

To assemble the Infusion Sample Delivery System 25

To infuse solution through the Infusion Sample Delivery System 26

To assemble the Extension Tube column support assembly for LC

analysis 27

To install the Needle Sleeve Assembly into the Nanospray Ion

Source 32

To install the Nanospray Slide Assembly for inline analysis 36

To adjust the LED camera light 41

1 Installation

8 Nanospray Ion Source User Guide

Upgrade Preparation

The installation kit contains several upgrade kits for older instruments:

•gas distributor (p/n G1964-20303)

•spray shield kit (p/n G1988-60007)

If you have an installed instrument, inspect the instrument to determine

which of these kits need to be installed. These parts are already installed on

new instruments.

You also need to adjust the position of the Counter Electrode. A 1.5 mm hex

wrench, and a 7 mm spacing tool are provided for setting the distance of the

Counter Electrode from the spray shield.

To remove the ESI or Agilent Jet Stream source

1Put the instrument into standby mode and lower the source temperatures

to start cooling down the source.

2When the source is cool, turn off the nebulizer, drying gas, and sheath gas

flow (if using the Jet Stream source).

3Remove the existing source, install the protective cover and store in a safe

place.

4Remove the spray shield and store with the source.

NOTE

Autotune is not supported with the Agilent G1992A Nanospray Ion Source. Before

changing the source, do all tuning with the ESI or Agilent Jet Stream source. Calibration

can still be done with the Nanospray Ion Source.

Installation 1

Nanospray Ion Source User Guide 9

To upgrade the gas distributor

Newer model gas distributors are required to support the improved resistive

or dielectric capillary. If your gas distributor looks like the one in Figure 1,

replace it with the new one.

1Remove the chamber and capillary high voltage cable leads from the T-nut

connectors.

Figure 1 Remove the four T20 Torx screws from the metal ring.

2Remove the four T-20 torx screws used to secure the metal ring that holds

the PTFE spray shield support block in place.

3Remove the PTFE spray shield support block to access the vespel gas

distributor. Remove and discard the old gas distributor.

1 Installation

10 Nanospray Ion Source User Guide

Figure 2 Remove the old gas distributor

4Install the new gas distributor (p/n G1964-20303) with the solid side facing

the PTFE wall. Position it so that it is flush with the PTFE plate.

Figure 3 G1964-20303 Gas Distributor

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