Agilent Technologies 7250 Manuale utente
Agilent 7250 Accurate-Mass Quadrupole
Time-of-Flight GC/MS System
Troubleshooting and Maintenance Manual
7250 Accurate-Mass Q-TOF Troubleshooting and Maintenance Manual
Notices
© Agilent Technologies, Inc. 2019
No part of this manual may be repro-
duced in any form or by any means
(including electronic storage and
retrieval or translation into a foreign lan-
guage) without prior agreement and
written consent from Agilent Technolo-
gies, Inc. as governed by United States
and international copyright laws.
Manual Part Number
G7250-90007
Edition
First Edition, January 2019
Printed in USA
Agilent Technologies, Inc.
5301 Stevens Creek Boulevard
Santa Clara, CA 95052
Warranty
The material contained in this docu-
ment is provided “as is,” and is subject
to being changed, without notice, in
future editions. Further, to the maxi-
mum extent permitted by applicable
law, Agilent disclaims all warranties,
either express or implied, with regard
to this manual and any information
contained herein, including but not lim-
ited to the implied warranties of mer-
chantability and fitness for a particular
purpose. Agilent shall not be liable for
errors or for incidental or consequen-
tial damages in connection with the
furnishing, use, or performance of this
document or of any information con-
tained herein. Should Agilent and the
user have a separate written agree-
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material in this document that conflict
with these terms, the warranty terms in
the separate agreement shall control.
Safety Notices
CAUTION
A CAUTION notice denotes a
hazard. It calls attention to an
operating procedure, practice,
or the like that, if not correctly
performed or adhered to, could
result in damage to the product
or loss of important data. Do
not proceed beyond a CAUTION
notice until the indicated
conditions are fully understood
and met.
WARNING
A WARNING notice denotes a
hazard. It calls attention to an
operating procedure, practice,
or the like that, if not correctly
performed or adhered to, could
result in personal injury or
death. Do not proceed beyond a
WARNING notice until the
indicated conditions are fully
understood and met.
7250 Accurate-Mass Q-TOF Troubleshooting and Maintenance Manual 3
Contents
1Introduction
Abbreviations Used 10
The 7250 Accurate-Mass Quadrupole Time-of-Flight GC/MS
System 11
7250 Accurate-Mass Q-TOF GC/MS Description 13
Side Panel AC Power Connectors 14
Back Panel Connectors 15
Interfacing Start Events to External Devices 16
Remote control processor 16
Remote start signals 16
System ready 16
Start run input 17
Important Safety Warnings 18
Safety and Regulatory Certifications 21
Intended Use 24
Cleaning/Recycling the Product 24
Accidental Liquid Spillage 24
Moving or Storing the MS 24
2 General Troubleshooting
Troubleshooting Tips and Tricks 26
General Symptoms 27
Chromatographic Symptoms 29
Mass Spectra General Symptoms 34
Pressure Symptoms 36
4 7250 Accurate-Mass Q-TOF Troubleshooting and Maintenance Manual
Temperature Symptoms 38
Error Messages 40
Air Leaks 45
Contamination 46
3 CI Troubleshooting
Common CI-Specific Problems 50
Troubleshooting Tips and Tricks 51
Air Leaks 52
How do I know if I have an air leak? 52
Special NCI notes 53
How do I find the air leak? 53
Pressure-Related Symptoms 55
Poor vacuum without reagent gas flow 56
High pressure with reagent gas flow 56
Pressure does not change when reagent flow is
changed 57
Signal-Related Symptoms 58
No peaks 58
No reagent gas peaks in PCI 58
No PFDTD peaks in PCI 59
No reagent gas peaks in NCI 59
No PFDTD calibrant peaks in NCI 59
No sample peaks in NCI 59
Large peak at m/z 238 in NCI OFN spectrum 60
No or low reagent gas signal 60
No or low PFDTD signal, but reagent ions are normal 61
Excessive noise or low signal-to-noise ratio 62
Peak at m/z 32 63
Tuning-Related Symptoms 64
7250 Accurate-Mass Q-TOF Troubleshooting and Maintenance Manual 5
Reagent gas ion ratio is difficult to adjust or unstable 64
Cannot complete Autotune 65
The CI ion source is dirty 65
Air leak 65
4 General Maintenance
Before Starting 68
Scheduled maintenance 68
Tools, spare parts, and supplies 69
High voltage precautions 69
Dangerous temperatures 69
Chemical residue 70
Electrostatic discharge 71
To Refill the EI Calibration Vial 73
To Connect the GC Nitrogen Gas Source to the Collision
Cell 75
To Separate the GC from the MS 76
To Position the GC Next to the MS 78
To Move or Store the MS 79
To Access the Left Side Lifting Handle 82
5 CI Maintenance
To Minimize Rotary Vane Foreline Pump Damage from
Ammonia 86
To Replace the Chemical Ionization Gas Purifier 88
To Clean the Reagent Gas Supply Lines 89
To Refill the CI Calibration Vial 90
6VacuumSystem
Overview 94
6 7250 Accurate-Mass Q-TOF Troubleshooting and Maintenance Manual
Maintaining the Vacuum System 95
Periodic maintenance 95
Other procedures 95
More information is available 95
Vacuum System Components 96
Common Vacuum System Problems 97
Foreline Pump 98
To connect the foreline hose to the foreline pump 98
To disconnect the foreline hose from the foreline
pump 99
To check the rotary vane pump oil mist filter 99
To check the rotary vane foreline pump fluid level 100
To add oil to the rotary vane foreline pump 100
To replace the oil in the rotary vane foreline pump 101
The oil pan under the foreline pump can be a fire
hazard 103
To remove the exhaust silencer filter 103
To install the exhaust silencer filter 104
To replace the IDP-15 dry pump tip seal 105
To replace the IDP pump HEPA filter cartridge 107
To change the exhaust silencer filter cartridge 107
To install the IDP pump ballast 108
To remove the IDP pump ballast 109
Side Plate 110
Vacuum Seals 110
EI Calibration Valve 111
CI Calibration Valve 112
7 Replacement Parts
To Order Parts 114
8 7250 Accurate-Mass Q-TOF Troubleshooting and Maintenance Manual
7250 Accurate-Mass Q-TOF Troubleshooting and Maintenance Manual 9
1Introduction
Abbreviations Used 10
The 7250 Accurate-Mass Quadrupole Time-of-Flight GC/MS System 11
7250 Accurate-Mass Q-TOF GC/MS Description 13
Side Panel AC Power Connectors 14
Back Panel Connectors 15
Interfacing Start Events to External Devices 16
Important Safety Warnings 18
Safety and Regulatory Certifications 21
Intended Use 24
Cleaning/Recycling the Product 24
Moving or Storing the MS 24
This chapter provides general information about the Agilent 7250 Accurate-Mass
Quadrupole Time-of-Flight (Q-TOF) GC/MS System, including a hardware
description and general safety warnings.
1Introduction
10 7250 Accurate-Mass Q-TOF Troubleshooting and Maintenance Manual
Abbreviations Used
The abbreviations in Table 1 are used in discussing this product. They are
collected here for convenience.
Table 1 Abbreviations
Abbreviation Definition
AC Alternating current
ALS Automatic liquid sampler
CC Collision cell
CI Chemical ionization
DC Direct current
EI Electron impact
EPC Electronic pneumatic control
eV Electron volt
GC Gas chromatograph
GC/MS Gas chromatograph mass spectrometer
id Inside diameter
IDP Isolated dry pump
LAN Local Area Network
LE-EI Low energy EI
m/z Mass-to-charge ratio
MFC Mass flow controller
MS Mass spectrometer
MS1 Quadrupole
OFN Octafluoronaphthalene (sample)
PFTBA Perfluorotributylamine (calibrant)
Q-TOF Quadrupole time-of-flight
Quad Quadrupole mass filter
RF Radio frequency
RFPA Radio frequency power amplifier
TOF Time-of-flight
Torr Unit of pressure, 1 mm Hg
Turbo Turbomolecular vacuum pump
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