Agilent Technologies 7250 Manuale utente

Agilent 7250 Accurate-Mass Quadrupole

Time-of-Flight GC/MS System

Troubleshooting and Maintenance Manual

7250 Accurate-Mass Q-TOF Troubleshooting and Maintenance Manual

Notices

No part of this manual may be repro-

duced in any form or by any means

(including electronic storage and

retrieval or translation into a foreign lan-

guage) without prior agreement and

written consent from Agilent Technolo-

gies, Inc. as governed by United States

and international copyright laws.

Manual Part Number

G7250-90007

Edition

First Edition, January 2019

Printed in USA

Agilent Technologies, Inc.

5301 Stevens Creek Boulevard 

Santa Clara, CA 95052

Warranty

The material contained in this docu-

ment is provided “as is,” and is subject

to being changed, without notice, in

future editions. Further, to the maxi-

mum extent permitted by applicable

law, Agilent disclaims all warranties,

either express or implied, with regard

to this manual and any information

contained herein, including but not lim-

ited to the implied warranties of mer-

chantability and fitness for a particular

purpose. Agilent shall not be liable for

errors or for incidental or consequen-

tial damages in connection with the

furnishing, use, or performance of this

document or of any information con-

tained herein. Should Agilent and the

user have a separate written agree-

ment with warranty terms covering the

material in this document that conflict

with these terms, the warranty terms in

the separate agreement shall control.

Safety Notices

CAUTION

A CAUTION notice denotes a 

hazard. It calls attention to an

operating procedure, practice,

or the like that, if not correctly

performed or adhered to, could

result in damage to the product

or loss of important data. Do

not proceed beyond a CAUTION

notice until the indicated

conditions are fully understood

and met.

WARNING

A WARNING notice denotes a

hazard. It calls attention to an

operating procedure, practice,

or the like that, if not correctly

performed or adhered to, could

result in personal injury or

death. Do not proceed beyond a

WARNING notice until the

indicated conditions are fully

understood and met.

7250 Accurate-Mass Q-TOF Troubleshooting and Maintenance Manual 3

Contents

1Introduction

Abbreviations Used 10

The 7250 Accurate-Mass Quadrupole Time-of-Flight GC/MS

System 11

7250 Accurate-Mass Q-TOF GC/MS Description 13

Side Panel AC Power Connectors 14

Back Panel Connectors 15

Interfacing Start Events to External Devices 16

Remote control processor 16

Remote start signals 16

System ready 16

Start run input 17

Important Safety Warnings 18

Safety and Regulatory Certifications 21

Intended Use 24

Cleaning/Recycling the Product 24

Accidental Liquid Spillage 24

Moving or Storing the MS 24

2 General Troubleshooting

Troubleshooting Tips and Tricks 26

General Symptoms 27

Chromatographic Symptoms 29

Mass Spectra General Symptoms 34

Pressure Symptoms 36

4 7250 Accurate-Mass Q-TOF Troubleshooting and Maintenance Manual

Temperature Symptoms 38

Error Messages 40

Air Leaks 45

Contamination 46

3 CI Troubleshooting

Common CI-Specific Problems 50

Troubleshooting Tips and Tricks 51

Air Leaks 52

How do I know if I have an air leak? 52

Special NCI notes 53

How do I find the air leak? 53

Pressure-Related Symptoms 55

Poor vacuum without reagent gas flow 56

High pressure with reagent gas flow 56

Pressure does not change when reagent flow is

changed 57

Signal-Related Symptoms 58

No peaks 58

No reagent gas peaks in PCI 58

No PFDTD peaks in PCI 59

No reagent gas peaks in NCI 59

No PFDTD calibrant peaks in NCI 59

No sample peaks in NCI 59

Large peak at m/z 238 in NCI OFN spectrum 60

No or low reagent gas signal 60

No or low PFDTD signal, but reagent ions are normal 61

Excessive noise or low signal-to-noise ratio 62

Peak at m/z 32 63

Tuning-Related Symptoms 64

7250 Accurate-Mass Q-TOF Troubleshooting and Maintenance Manual 5

Reagent gas ion ratio is difficult to adjust or unstable 64

Cannot complete Autotune 65

The CI ion source is dirty 65

Air leak 65

4 General Maintenance

Before Starting 68

Scheduled maintenance 68

Tools, spare parts, and supplies 69

High voltage precautions 69

Dangerous temperatures 69

Chemical residue 70

Electrostatic discharge 71

To Refill the EI Calibration Vial 73

To Connect the GC Nitrogen Gas Source to the Collision

Cell 75

To Separate the GC from the MS 76

To Position the GC Next to the MS 78

To Move or Store the MS 79

To Access the Left Side Lifting Handle 82

5 CI Maintenance

To Minimize Rotary Vane Foreline Pump Damage from

Ammonia 86

To Replace the Chemical Ionization Gas Purifier 88

To Clean the Reagent Gas Supply Lines 89

To Refill the CI Calibration Vial 90

6VacuumSystem

Overview 94

6 7250 Accurate-Mass Q-TOF Troubleshooting and Maintenance Manual

Maintaining the Vacuum System 95

Periodic maintenance 95

Other procedures 95

More information is available 95

Vacuum System Components 96

Common Vacuum System Problems 97

Foreline Pump 98

To connect the foreline hose to the foreline pump 98

To disconnect the foreline hose from the foreline

pump 99

To check the rotary vane pump oil mist filter 99

To check the rotary vane foreline pump fluid level 100

To add oil to the rotary vane foreline pump 100

To replace the oil in the rotary vane foreline pump 101

The oil pan under the foreline pump can be a fire

hazard 103

To remove the exhaust silencer filter 103

To install the exhaust silencer filter 104

To replace the IDP-15 dry pump tip seal 105

To replace the IDP pump HEPA filter cartridge 107

To change the exhaust silencer filter cartridge 107

To install the IDP pump ballast 108

To remove the IDP pump ballast 109

Side Plate 110

Vacuum Seals 110

EI Calibration Valve 111

CI Calibration Valve 112

7 Replacement Parts

To Order Parts 114

7250 Accurate-Mass Q-TOF Troubleshooting and Maintenance Manual 7

Electronics 115

Vacuum System 118

Analyzer 121

GC/MS Interface 126

Consumables and Maintenance Supplies 128

Additional CI Parts 131

8 7250 Accurate-Mass Q-TOF Troubleshooting and Maintenance Manual

7250 Accurate-Mass Q-TOF Troubleshooting and Maintenance Manual 9

1Introduction

Abbreviations Used 10

The 7250 Accurate-Mass Quadrupole Time-of-Flight GC/MS System 11

7250 Accurate-Mass Q-TOF GC/MS Description 13

Side Panel AC Power Connectors 14

Back Panel Connectors 15

Interfacing Start Events to External Devices 16

Important Safety Warnings 18

Safety and Regulatory Certifications 21

Intended Use 24

Cleaning/Recycling the Product 24

Moving or Storing the MS 24

This chapter provides general information about the Agilent 7250 Accurate-Mass

Quadrupole Time-of-Flight (Q-TOF) GC/MS System, including a hardware

description and general safety warnings.

1Introduction

10 7250 Accurate-Mass Q-TOF Troubleshooting and Maintenance Manual

Abbreviations Used

The abbreviations in Table 1 are used in discussing this product. They are

collected here for convenience.

Table 1 Abbreviations

Abbreviation Definition

AC Alternating current

ALS Automatic liquid sampler

CC Collision cell

CI Chemical ionization

DC Direct current

EI Electron impact

EPC Electronic pneumatic control

eV Electron volt

GC Gas chromatograph

GC/MS Gas chromatograph mass spectrometer

id Inside diameter

IDP Isolated dry pump

LAN Local Area Network

LE-EI Low energy EI

m/z Mass-to-charge ratio

MFC Mass flow controller

MS Mass spectrometer

MS1 Quadrupole

OFN Octafluoronaphthalene (sample)

PFTBA Perfluorotributylamine (calibrant)

Q-TOF Quadrupole time-of-flight

Quad Quadrupole mass filter

RF Radio frequency

RFPA Radio frequency power amplifier

TOF Time-of-flight

Torr Unit of pressure, 1 mm Hg

Turbo Turbomolecular vacuum pump

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