Artel PCS Manuale utente

Po ering

On & Off

NOTE:

f the STAR Dot Matrix Printer is being used, please

refer to the STAR Printer User’s Manual for printer

connection and operation.

Powering On

Connect system according to diagram below.

Before performing this procedure, ensure that the

Reagent Kit is at the same temperature as the

nstrument.

• Switch the nstrument and the printer to ON. (The

nstrument is on when the “ ” on the rocker switch is

depressed.) The nstrument will perform a system test.

Be sure that the printer is ON L NE.

• Clean vials if necessary.

• Load CAL A and Blank vials into the vial block at the

back of the nstrument at least 15 minutes prior to use.

Powering Off

• Remove all vials from the nstrument.

• Switch the printer to OFF.

• Switch the nstrument to OFF.

• Store all reagents according to storage requirements.

Power switch

Via b ock

The co or-coded bars within the text throughout this Guide indicate

the sections where specific instructions are given.

PCS uickstart-15A3179_PCS uickstart 15A3179 Rev 4/3/13 2:01 PM Page 2

Always refer to the PCS Procedure Guide for further instruction

Po ering

On & Off

PCS uickstart-15A3179_PCS uickstart 15A3179 Rev 4/3/13 2:01 PM Page 3

Seiko

Printer

Cont ols and Indicato s:

Paper Feed Switch: Pressing this switch feeds the

paper continuously. Paper feed will only work when

the OFF L NE indicator illuminates or flashes.

Power Switch: When the switch is in the ON position,

power is supplied to the printer and the POWER and

ON L NE indicators illuminate. When the printer is out

of paper, the OFF L NE indicator flashes.

PCS uickstart-15A3179_PCS uickstart 15A3179 Rev 4/3/13 2:01 PM Page 4

Always refer to the PCS Procedure Guide for further instruction

Seiko

Printer

Loading the P inte Pape :

• Turn the power switch to the ON position.

• Open the paper cover located on the top, right side of the

printer.

• Cut the end of the paper roll cleanly and squarely.

• nsert the end of the paper into the inlet at the bottom of the

paper holder until the auto-loader catches it and feeds the

paper through.

• The outside of the roll is the printable surface for the

thermal paper roll.

• Close the paper cover.

PCS uickstart-15A3179_PCS uickstart 15A3179 Rev 4/3/13 2:01 PM Page 5

Consumable

Components

Vial Cleaning

To ensure accurate results, all vials should be free

of fingerprints and smudges prior to placing in the

vial block for equilibration. All vials should be

cleaned prior to use.

• Holding the vial by the cap, immerse the lower

portion of the vial (about 2.5 cm) into clean

isopropyl alcohol.

• Remove the vial from the alcohol and gently

wipe the lower portion of the vial by rotating the

vial against a lint-free wipe.

Disposal

• All PCS reagents are non-hazardous and aqueous

based.

• Small quantities may be disposed of in sewer. Large

quantities should be disposed of in accordance with

local, state or federal regulations.

f Reagent Kit has been moved from another location,

depending on the difference in temperature between

the two locations, an hour may be required for

equilibration.

Removing the kit components from the box and

letting them stand in free air will hasten equilibration.

Sto age

• The Reagent Kit should be stored at room temperature

(18-28° C).

• Avoid temperatures exceeding 32° C.

• Kits should be stored in the dark to the extent practical.

• Frozen reagents should be brought to room temperature

and inverted several times prior to use.

PCS uickstart-15A3179_PCS uickstart 15A3179 Rev 4/3/13 2:02 PM Page 6

Always refer to the PCS Procedure Guide for further instruction

Consumable

Components

Reagent Kit

• B ank Reagent Via s:

Standard Reagent Kits contain 12 vials pre-filled with

Blank Solution.

Bulk Reagent Kits contain 50 vials pre-filled with Blank

Solution.

• Samp e So utions:

Range 1: For pipette volumes 200 – 5000 µL

Range 2: For pipette volumes 50 – 199 µL

Range 3: For pipette volumes 10 – 49 µL

Range 4: For pipette volumes 2 – 9 µL

Range 5*: For pipette volumes 0.5 – 1.9 µL

Range 6*: For pipette volumes 0.1 – 0.49 µL

•Samp e A iquot Containers:

Use aliquot containers to hold Sample Solutions used

during calibration to avoid contamination of Sample

Solution bottle.

•Transfer Pipettes:

Plastic transfer pipettes are used to transfer Sample

Solution from the reagent bottle to the aliquot container.

•Ca ibration Standard:

CAL A vial is used to collect a zero reading for the

nstrument.

•Kit Inserts:

Declaration of Traceability

*Range 5 and 6 are sold separately.

Lot code card

PCS uickstart-15A3179_PCS uickstart 15A3179 Rev 4/3/13 2:02 PM Page 7

Pipette

Calibration

Setup

Sta ting a Pipette Calib ation

• At the Main Menu prompt (Figure 1), press YES to

begin the pipette calibration procedure. The nstrument

will perform a temperature check.

• The nstrument will prompt for lot code (Figure 2). The

nstrument retains up to 6 previously entered lot codes.

f the entered code matches one previously entered,

press NO and re-enter the code.

• Press YES if entering a new lot code. Group numbers

found on the correct side of the lot code card must be

entered. Press ENTER after each group of digits is

entered.

• A confirmation prompt will be displayed (Figure 3).

Press YES.

• The nstrument will perform a brief self-calibration.

mmediately following the self-calibration the nstrument

will prompt to insert the CAL A vial (Figure 4).

• Open the vial holder cover and insert the CAL A vial

pressing down until it meets the bottom of the vial

holder.

• The nstrument will then perform a zero check and

prompt to remove the CAL A when the test is complete.

Return the CAL A vial to the Reagent Kit for future use.

Main Menu

Pipette Calibration?

Figure 1

Enter Reagent Lot Code:

Lot Code XXXXX

Correct? (Y/N)

Figure 2

Figure 3

Insert

Cal A Vial

Figure 4

PCS uickstart-15A3179_PCS uickstart 15A3179 Rev 4/3/13 2:02 PM Page 8

Always refer to the PCS Procedure Guide for further instruction

Important Notes:

• Use the CAL A vial from the Reagent Kit and not from the nstrument Calibrator Kit.

• Never remove the cover of the CAL A vial.

• The label for the CAL A vial should face the front of the nstrument with the vertical line facing forward.

• Each Blank vial contains a predetermined volume of solution. Any spillage will reduce accuracy.

• The lower portion of the Blank vial must be free of smudges. Touching this area should be avoided.

• Once the Blank vial has been inserted into the nstrument, it must not be turned or moved until the calibration

procedure is complete for that vial.

• The nstrument will then prompt to insert a blue Blank

vial into the vial holder (Figure 5). Remove the Blank vial

from the vial block and remove the cap.

• nsert the open vial into the vial holder pressing down

until it contacts the bottom of the vial holder.

• Close the nstrument cover. The nstrument will read the

Blank vial.

Remove Cap and

Insert Blank

Figure 5

PCS uickstart-15A3179_PCS uickstart 15A3179 Rev 4/3/13 2:02 PM Page 9

• The nstrument will prompt to enter an operator D

(Figure 6). Enter a number (not exceeding 20 digits) to

identify the operator. For alpha character entry, refer to

Appendix E of the PCS Procedure Guide.

• Enter the pipette D (Figure 7). Type in a number not

exceeding 20 digits and press ENTER. The printer will

print the calibration header information.

• Enter the volume (in microliters) to be dispensed and

press ENTER (Figure 8). The CLEAR key can be used

to correct any typing errors.

Enter Operator ID:

Enter Pipette ID:

Enter Pipette

Volume: ___ µL

Figure 6

Figure 7

Figure 8

PCS uickstart-15A3179_PCS uickstart 15A3179 Rev 4/3/13 2:02 PM Page 10

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