Biosan UVC-AR Manuale utente

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Please send all feedback to:

Manufacturer:

SIA Biosan

Ratsupites iela 7 k-2, Riga, LV-1067, Latvia

Phone: +371 674 261 37

Fax: +371 674 281 01

www.biosan.lv

Marketing:marketing@biosan.lv

Service: service@biosan.lv

Contents

1. About this edition of user instructions.......................................................................3

2. Safety precautions ...................................................................................................3

3. General information..................................................................................................5

4. Getting started..........................................................................................................6

5. Operation .................................................................................................................7

6. Specifications...........................................................................................................9

7. Ordering information ..............................................................................................11

8. Care and maintenance...........................................................................................12

9. Storage and transportation.....................................................................................14

10. Warranty.................................................................................................................14

11. EU Declaration of conformity..................................................................................15

1. About this edition of user instructions

The current edition of the user instructions applies to the following models:

Model

Version

UVC/T-AR, DNA/RNA UV-cleaner box

V.3AD

UVC/T-M-AR, DNA/RNA UV-cleaner box

V.6A02, V.6A03, V.6A04, V.6AI and V.6A12

UVT-B-AR, DNA/RNA UV-cleaner box

V.4AB, V.4AD, V.4AE, V.4AI and V.4A12

UVT-S-AR, DNA/RNA UV-cleaner box

V.5AA, V.5AB, V.5A7, V.5AI and V.5A04

2. Safety precautions

Caution!

Make sure you have fully read and understoodpresent instructions before

using the equipment. Please pay special attention to sections marked by

this symbol.

Caution!

The front protective screen must be closed while the open UV lamp is

switched on. Otherwise, the operator can be exposed to a dangerous

level of UV emission.

Caution!

The unit contains a source of UV radiation. Do not start the unit without

the cover of the UV recirculator. Exposure to UV light is harmful and can

cause damage to unprotected eyes and skin.

Caution!

The UV-Cleaner box contains a powerful source of UV radiation, there-

fore, before operating the unit, ensure all personnel working with the

UV-Cleaner box are properly protected. The operator should wear a

closed-front lab coat (fully buttoned), UV certified safety glasses and

gloves, which should overlap the lab coat or surgical gown cuffs.

2.1 General safety

•The protection provided by the equipment may be impaired if the equipment is used

with accessories (shelves, furniture) not provided or recommended by the manufac-

turer, or used in a manner not specified by the manufacturer.

•Save the unit from shocks and falling.

•Do not use the unit if it has visible mechanical damage.

•Store and transport the unit as descried in the Storage and transportation section.

•Before using any cleaning or decontamination methods except those recommended

by the manufacturer, check with the manufacturer that the proposed method will not

damage the equipment.

•Do not make modifications in design of the unit.

•When handling hazardous substances, follow the instructions contained in the safety

data sheets for the individual substances that are used and observe the relevant ac-

cident prevention regulations.

2.2 Electrical safety

•Connect only to the mains with voltage corresponding to that on the serial number

label.

•Do not plug the unit into an ungrounded powersocket, and donot use an ungrounded

extension lead.

•Ensure that the power plug is easily accessible during use.

•Disconnect the unit from the mains before moving.

•If liquid penetrates into the unit, disconnect it from the mains and have it checked by

a repair and maintenance technician.

•Do not operate the unit in premises where condensation can form. Operating condi-

tions of the unit are defined in the Specifications section.

2.3 During operation

•Do not operate the unit in environments with aggressive or explosive chemical mix-

tures. Please contact manufacturer for possible operation of the unit in specific at-

mospheres.

•Do not operate the unit if it is faulty or has been installed incorrectly.

•Do not use outside laboratory rooms.

•Do not operate the unit without dust filters installed.

•Do not work in the box while the open UV lamp is switched on.

2.4 Biological safety

•The user is responsible to carry out appropriate decontamination if hazardous mate-

rial spills on or penetrates into the equipment.

•The user is responsible for decontamination of the unit before its decommissioning

and utilization.

3. General information

DNA/RNA UV-Cleaner boxes –UVC/T-AR, UVC/T-M-AR, UVT-B-AR and UVT-S-

AR –are designed for clean operations with DNA/RNA samples. They provide protection

against contamination.

All models are bench-top type, with metal framework, glass or acrylic walls and work-

ing surface painted with powder enamel or made of stainless steel. Box is equipped with

inlet for power cables and built-in power outlets for units inside the box. For availability of the

required characteristics, please clarify for each model separately (see 7.1 on page 11).

UV-Cleaner boxes are equipped with an open UV lamp installed in the upper hood.

UV radiation from the open lamps disinfects the working area inactivating DNA/RNA frag-

ments during 30 min of exposure. A digital timer controls duration of the direct UV irradiation.

A daylight lamp provides proper illumination of the working surface.

UV-Cleaner box is equipped with a flowing bactericidal UV-cleaner-recirculator AR,

which provides constant decontamination inside the box during operation. It is recommended

for operations with DNA/RNA amplicons.

UV recirculator consists of an UV lamp (fig. 1/1), a fan and dust filters (fig. 1/2) orga-

nized in a plastic case. Operator working in a UV-Cleaner box with a switched on UV recir-

culator is not exposed to UV radiation. It allows continuous treatment of the airflow with UV

light without interrupting working process. Air circulation at a short distance from the UV lamp

combined with reflective surfaces in the air duct results in increased density of UV rays lead-

ing to higher efficiency of disinfection. UV recirculator generates 100volumes ofPCR cabinet

per one hour of airflow exchange ensuring maximally aseptic conditions inside the cabinet.

Box for clean operations with DNA/RNA samples with built-in recirculator is a pa-

tented solution (patent LV13115 from 20/05/2004, Dr. biol. V. Bankovsky).

Microbiological studies at the R&D Department of Biosan led by Dr. biol. V. Bankov-

sky demonstrated a high level of biosafety and efficacy of UV-cleaner box (maximal level of

contamination is 1-3 cfu per 100 litres of air)

UV-Cleaner box is designed to biologically protect the product, but not the operator,

therefore, it is not recommended to use the UV-Cleaner box for working with pathogenic

microorganisms of biosafety level BSL-II and higher, without specialized protection.

Advantages:

•UV-recirculator;

•Ozone free high density UV decontamination;

•Open UV lamp is switched off automatically in case of opening the front screen;

•Long life UV lamps (9000 h);

•Low noise level and energy consumption;

•Compact tabletop model for personal laboratories;

•Shelves for pipettes and reagents (on request)

•Table with drawer T-4 / T-4L, laboratory chest of drawers LF-1 (on request).

See http://biosan.lv/eval-en

Figure 1. Recirculator scheme.

1. UV lamp. 2. Fan and input filter. 3. UV indicator. 4. Output filter

4. Getting started

4.1 Unpacking. Remove packing materials carefully and retain them for future shipment

or storage of the unit. Examine the unit carefully for any damage incurred during

transit. The warranty does notcover in-transit damage. Warranty covers only the units

transported in the original package.

Caution!

Due to the high weight of the unit, its unpacking and installing must be

carried out by two persons. Follow the instructions included on the pack-

aging.

4.2 Complete set. Package contents:

4.2.1 Standard set:

- DNA/RNA UV-cleaner box ...............................................................................1 pce.

- Power cable (UVC/T-AR only)..........................................................................1 pce.

- Spare fuse for control block

............................................................................1 pce.

- Spare fuse for built-in power outlet

.................................................................1 pce.

- Spare dust filters..............................................................................................2 pcs.

- User instructions, declaration of conformity ....................................................1 copy

4.2.2 Optional accessories:

- P-5, shelf for pipettes................................................................................on request

- F-1, shelf for reagents and samples..........................................................on request

- PDS-250, DNA/RNA Decontamination Solution Spray, 250 ml.................on request

- T-4 / T-4L moving table for UV-Cleaner box..............................................on request

- LF-1, laboratory chest of drawers..............................................................on request

P-5 F-1 PDS-250

For information on fuse replacement, see 8.3 on page 12

For all models, excluding UVC/T-AR, see 7.1 on page 11

T-4 T-4L LF-1

4.3 Setup.

- If the moving table is used, unpack it carefully and assemble according to the en-

closed assembling scheme.

- Place the unit upon stable surface. Ensure that the unit is placed on a solid, level

surface not less than 720x550 mm (1290x600 mm for UVT-S-AR model), which is

able to support its weight and the weight of equipment and materials inside, for in-

stance on the T-4 / T-4L table.

- Position the unit with easy access to the power switch and plug.

4.4 Shelf setup. Refer to the instructions included with the shelf.

5. Operation

Note.

For UVC/T-M-AR and UVT-B-AR boxes, check that the short power cable

(fig. 5/1) is connecting the fuse box and the controller top.

5.1 Connect the power plug to a grounded power socket. Turn on the Power switch:

•UVC/T-AR –no switch, the unit powers up automatically;

•UVC/T-M-AR, UVT-B-AR –on the right side of the unit, behind the rear wall (fig. 5/3);

•UVT-S-AR –on the left side of the unit, behind the rear wall (fig. 6/1).

5.2 UV exposition of the working place.

Caution!

The front protective screen must be closed while the open UV lamp is

switched on. Otherwise, the operator can be exposed to a dangerous

level of UV emission.

5.2.1 Turn ON the switch 2(fig. 2/2) on the control panel (fig. 3/1). This switches on the UV

recirculator inside the unit (fig. 3/2) and the open UV-lamp exposition timer. The UV

recirculator will operate all the time until the switch 2is turned OFF.

Figure 2. Control panel

Note.

Open UV lamp operation can be checked with the visible light lamp

switched off (switch 1is OFF). Use the indicator in the centre of recircu-

lator cover (fig. 1/3) to check operation of UV lamp inside the recirculator.

If indicator lights from inside while switch 2is ON, then the UV lamp is

functioning.

5.2.2 Use the timer +and -keys (fig. 2/4) to increase and decrease the time (SETTING

TIME) of direct UV light exposition of the working place, with 1 min increment. Press-

ing down and holding the button for more than 2 seconds increases the increment.

Recommended time of exposition is two sessions of 30 minutes, once after switching

on the cabinet, once before operations inside.

Note.

When using the P-5 and F-1 shelves, extend the exposition time to two

sessions of 45 minutes.

5.2.3 Press the Start key (fig. 2/3), the UV lamp will be turned on automatically and the

timer will start counting the exposition time. Timer indicator shows actual time: until 1

hour - in minutes and seconds (mm:ss), after 1 hour - in hours and minutes (hh:mm).

After reaching the set time the timer will automatically turn off the open UV lamp.

5.2.4 The open UV lamp can be switched off by pressing the Stop/Reset key (fig. 2/5). The

set time of exposition will be saved in the memory. The set time will not be saved

after the complete turning off the unit.

5.2.5 If the set time of open UV light exposition is 0:00, pressing the Start key will make

the unit operate continuously during 24 hrs or until the Stop/Reset key is pressed.

5.3 The box is ready for operations. Work in the box.

Note.

Opening the front protective screen will switch off the open UV lamp au-

tomatically, but the timer will continue counting the exposition time.

5.3.1 Turn ON switch 1(fig. 2/1) for lighting of the working place. This turns ON the lumi-

nescent (visible light) lamp inside the cabinet (fig. 3/3).

5.3.2 Lift the front protective screen (fig. 3/5) up for work in the cabinet. Models have dif-

ferent maximum opening heights (fig. 3/8), as listed in the Specifications section,

and may be raised to the following positions:

•Model UVC/T-AR has a single opening height;

•Models UVC/T-M-AR, UVT-B-AR and UVT-S-AR has three protective screen posi-

tions –one-third, two-thirds and full opening height.

Note.

Do not block the recirculator openings (fig. 3/2)!

5.3.3 To use electric devices inside the cabinet, pull their power cable through the inlet and

close the flap (fig. 3/6) or connect the power cable to a built-in mains socket (fig. 3/7,

models with mains socket).

Caution!

Overall consumed power of devices connected through internal mains

sockets should not exceed 1000 W for 230V, or 600 W for 100–120 V.

5.4 After the task is done, close the front protective screen.

5.5 After finishing the operation turn OFF switch 2and switch 1. Turn off the Power

switch, if present. Disconnect the power cable from the mains.

UVC/T-AR, UVC/T-M-AR, UVT-B-AR UVT-S-AR

Figure 3. DNA/RNA UV box, frontal view:

1. Control panel. 2. Recirculator openings. 3. Daylight lamp and open UV lamp(s).

4. Upper front panel. 5. Movable front protective screen (opened). 6. Cable inlet.

7. Power outlet(s) (not in UVC/T-AR). 8. Opening height.

6. Specifications

The unit is designed for operation in cold rooms and closed laboratory rooms at am-

bient temperature from +4°C to +40°C in a non-condensing atmosphere and maximum rel-

ative humidity 80% for temperatures up to 31°C decreasing linearly to 50% relative humidity

at 40°C.

Biosan is committed to a continuous programme of improvement and reserves the

right to alter design and specifications of the equipment without additional notice.

Model

UVC/T-AR

Compact

UVC/T-M-AR

Compact

UVT-B-AR

Compact

UVT-S-AR

Double

Back panel

PMMA

Steel

Side panels

Glass

Coated steel

Glass

Front panel and screen

Glass

Working surface

Coated steel

Steel

Open UV lamp

1х TUV 25W G13 UV-C

2х TUV 30W G13 UV-C

UV intensity

18 mW/cm2/s

Radiation type

Ultraviolet light (λ=253.7 nm), no ozone

Time setting of direct

UV exposure

Digital timer, 1 minute - 24 hours / non-stop (increment 1 minute)

UV recirculator lamp

1х TUV 25W G13 UV-C

1х TUV 30W G13 UV-C

Visible light lamp

1х TLD 15W G-13

1х TLD 30W G-13

Thickness of side panels

4 mm

2 mm

4 mm

Thickness of upper front panel

8 mm

Thickness of protective screen

8 mm

4 mm

5 mm

Optical transparency

92%

95%

UV protection

> 99.90%

> 96%

Model

UVC/T-AR

Compact

UVC/T-M-AR

Compact

UVT-B-AR

Compact

UVT-S-AR

Double

Noise level

< 45 dB

< 47 dB

Working surface

490x645 mm

500x1210 mm

Opening height

165 mm

190 mm (max 210 mm)

190 mm (max 220 mm)

Opening levels

Full height

1/3, 2/3 and full height

Power inside the box (see 7.1)

Cable inlet

Cable inlet and 1 socket1

Cable inlet and 3 sock-

ets4

Operating current

100–240 V, 50/60 Hz

Power consumption

67 W

135 W

Dimensions

720x535x555

700x580x555 mm

1250 x 600 x 590 mm

Weight (net/gross)2

23 / 33 kg

28.8 / 39 kg

31.2 / 42 kg

58 / 68.5 kg

Laboratory table

T-4

T-4L

6.1 Used materials.

•PMMA –polymethylmethacrylate (acrylic) glass, Altuglas®EX.

•Glass –clear film (0.1 mm) coated glass, Euroglas®.

•Steel –stainless polished steel.

•Coated steel –white enamel powder coated steel.

6.2 Cabinet dimensions. Outer and inner dimensions, opening heights.

UVC/T-AR UVC/T-M-AR, UVT-B-AR

UVT-S-AR

Warning! Overall consumed power of devices connected through internal mains sockets should not

exceed 1000 W for 230V, or 600 W for 100–120 V

Accurate within ±10%

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