Waters 2545 Binary Gradient Module Manuale
Waters 2545 Binary
Gradient Module
Operator’s Guide
71500134902 / Revision A
Copyright © Waters Corporation 2006
All rights reserved
ii
Copyright notice
© 2006 WATERS CORPORATION. PRINTED IN THE UNITED STATES OF
AMERICA AND IRELAND. ALL RIGHTS RESERVED. THIS DOCUMENT
OR PARTS THEREOF MAY NOT BE REPRODUCED IN ANY FORM
WITHOUT THE WRITTEN PERMISSION OF THE PUBLISHER.
The information in this document is subject to change without notice and
should not be construed as a commitment by Waters Corporation. Waters
Corporation assumes no responsibility for any errors that may appear in this
document. This document is believed to be complete and accurate at the time
of publication. In no event shall Waters Corporation be liable for incidental or
consequential damages in connection with, or arising from, its use.
Trademarks
Waters is a registered trademark, and AutoPurification, FractionLynx, and
MassLynx are trademarks of Waters Corporation.
Tefzel and Teflon are registered trademarks of E. I. du Pont de Nemours and
Company.
Other trademarks or registered trademarks are the sole property of their
respective owners.
Customer comments
Waters’ Technical Communications department invites you to tell us of any
errors you encounter in this document or to suggest ideas for otherwise
improving it. Please help us better understand what you expect from our
documentation so that we can continuously improve its accuracy and
usability.
We seriously consider every customer comment we receive. You can reach us
at tech_comm@waters.com.
Waters Corporation
34 Maple Street
Milford, MA 01757
USA
iii
Safety considerations
Some reagents and samples used with Waters instruments can pose chemical,
biological, or radiological hazards. Be sure you are aware of the potentially
hazardous effects of all substances you work with. Always follow Good
Laboratory Practice, and consult your organization’s safety representative for
guidance.
When you develop methods, follow the “Protocol for the Adoption of Analytical
Methods in the Clinical Chemistry Laboratory,” American Journal of Medical
Technology, 44, 1, pages 30–37 (1978). This protocol addresses good operating
procedures and the techniques necessary to validate system and method
performance.
Instrument-specific safety considerations
High voltage hazard
Safety advisories
Consult Appendix A for a comprehensive list of warning and caution
advisories.
Warning: To avoid electric shock, do not remove the 2545 Binary
Gradient Module’s protective panels. The components they cover
are not user-serviceable.
iv
Operating this device
When operating this device, follow standard quality control procedures and
the guidelines presented in this section.
Intended use
Waters®designed the 2545 Binary Gradient Module to manage solvents under
MassLynx™software control in Waters AutoPurification Systems.
Calibration
To calibrate HPLC systems, follow acceptable calibration methods using at
least five standards to generate a standard curve. The concentration range for
standards should cover the entire range of quality-control samples, typical
specimens, and atypical specimens.
To calibrate mass spectrometers, consult the calibration section of the
operator’s guide of the instrument you are calibrating.
Quality control
Routinely run three quality-control samples that represent subnormal,
normal, and above-normal levels of a compound. Ensure that quality-control
sample results fall within an acceptable range, and evaluate precision from
day to day and run to run. Data collected when quality control samples are out
of range might not be valid. Do not report these data until you are certain that
the instrument performs satisfactorily.
Warning: Although some of the modules in the AutoPurification
System might carry IVDD labels, the system as a whole is not
intended for clinical use.
Table of Contents v
Safety considerations ......................................................................................... iii
Operating this device ......................................................................................... iv
1 Setting up the 2545 Binary Gradient Module .................................. 1-1
Features .............................................................................................................. 1-2
Installing the binary gradient module ........................................................ 1-4
Unpacking and inspecting .............................................................................. 1-6
Making power connections ............................................................................. 1-7
Making plumbing connections ...................................................................... 1-9
Making signal connections ........................................................................... 1-16
2 Preparing for operation ....................................................................... 2-1
Powering on ....................................................................................................... 2-2
Scanning connected instruments .................................................................. 2-3
Configuring 515 pumps .................................................................................... 2-6
Creating the inlet method ............................................................................... 2-8
Defining the gradient ..................................................................................... 2-10
Entering timed events ................................................................................... 2-14
Setting up analog signals .............................................................................. 2-16
Preparing solvent reservoirs ....................................................................... 2-17
3 Maintenance ............................................................................................ 3-1
Maintenance considerations .......................................................................... 3-2
Removing the pump head and seal-wash assemblies ............................... 3-4
Replacing the seals and O-ring .................................................................... 3-10
Table of Contents
vi Table of Contents
Replacing the plunger .................................................................................. 3-16
Replacing an inlet check valve cartridge ................................................. 3-20
Replacing an outlet check valve cartridge ............................................... 3-22
Replacing the vent valve rotor-seal assembly .......................................... 3-24
Diagnostics and configuration .................................................................... 3-28
Performing the leak test ............................................................................... 3-29
4 Troubleshooting ..................................................................................... 4-1
System troubleshooting .................................................................................. 4-2
Alarm reset ......................................................................................................... 4-4
Error messages .................................................................................................. 4-5
Troubleshooting hardware ............................................................................. 4-8
A Safety Advisories .................................................................................. A-1
Warning symbols ............................................................................................... A-2
Caution symbol .................................................................................................. A-6
Warnings that apply to all Waters instruments ......................................... A-7
Electrical and handling symbols ................................................................. A-14
B Specifications ........................................................................................ B-1
Physical specifications ................................................................................... B-2
Environmental specifications ....................................................................... B-3
Electrical specifications ................................................................................. B-4
Solvent management system specifications .............................................. B-7
Index ..................................................................................................... Index-1
1-2 Setting up the 2545 Binary Gradient Module
Features
The Waters®2545 Binary Gradient Module is a high-flow, high-pressure
mixing binary-gradient pump that delivers solvent for the Waters
AutoPurification™ System.
Based on Waters high-performance solvent management technology, the
binary gradient module provides consistently smooth, pulse-free solvent flow
for both analytical and preparative flow rates. The binary gradient module is
capable of pumping four solvents. Only two solvents can be programmed for
any gradient.
Pump systems A and B
Each binary gradient module consists of two independent pump systems,
A (top) and B (bottom). Each independent pump system contains two
linear-drive actuators (left and right). Each left and right actuator pair
contributes equally (in parallel) to deliver precise flows of a single solvent.
A
B
Features 1-3
The two independent pump systems combine their two solvents at a filter/tee,
where the solvent mix is plumbed to the sample manager or another
component of the AutoPurification System.
MassLynx™ software controls the ratio of the two solvents by varying the
relative flows of pump A and pump B. The binary gradient module receives
pressure data from a pressure transducer associated with each pump head.
The firmware measures the individual pump head pressure during the
pumping cycle and adjusts the precompression to ensure consistent solvent
delivery and minimize pump-induced detector baseline disturbances.
The MassLynx interface with the binary gradient module also provides useful
diagnostics and troubleshooting routines.
Leak management
All fluid-handling areas of the binary gradient module contain leak routing
capability. Spilled solvent is routed to the drain tube below the front panel.
The optional leak sensor should be installed on the leak sensor bracket in the
drip tray.
See also: Waters AutoPurification System Guide and Waters System Fluidics
Organizer Operator’s Guide.
1-4 Setting up the 2545 Binary Gradient Module
Installing the binary gradient module
To install the binary gradient module, you should know how to set up and
operate general laboratory instruments and computer-controlled devices, and
how to handle solvents.
See also: Contacting Waters Technical Service on page 3-2.
Required materials
• Flat-blade screwdriver (included in Startup Kit)
• 1/2-inch open-end wrench (included in Startup Kit)
• 5/16-inch open-end wrench (included in Startup Kit)
Site requirements
Factor Requirement
Internal cooling DC-powered fans that provide internal cooling by
pulling air through the binary gradient module
and exhausting it out the rear of the unit.
Airflow requirements Air enters the front of the binary gradient module
so pumps and other instrumentation can be placed
side by side.
Clearance At least 6 inches (15 cm) between the rear of the
binary gradient module and the wall to allow for
electrical connections and the ventilation fans’
exhaust.
Operating temperature 4 to 40 °C (39.2 to 104 °F)
Humidity 20% to 80%, noncondensing
Instrument-generated
acoustic noise <60 dBA at idle
Height 17 inches (43 cm)
Width 11 inches (28 cm)
Depth 31 inches (79 cm) with cables connected at the
rear; includes heads and tubes at the front
Weight 125 pounds (57 kg)
Line voltage 100 to 240 Vac ±10 V
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