Waters 2489 Manuale

2489 UV/Visible

Detector

Operator’s Guide

715003800 / Revision A

This operator's guide is intended for use with the 2489 UV/Visible detector,

part number 186248900, as pictured above. For the documentation to support

the 2489 UV/Visible detector, part number 186002489, see the operator's

guide, part number 71500142102.

Note: To locate the part number for your device, see the serial number label on

the back of the instrument.

ii November 9, 2012, 715003800 Rev. A

November 9, 2012, 715003800 Rev. A iii

General Information

DOCUMENT OR PARTS THEREOF MAY NOT BE REPRODUCED IN ANY

FORM WITHOUT THE WRITTEN PERMISSION OF THE PUBLISHER.

The information in this document is subject to change without notice and

should not be construed as a commitment by Waters Corporation. Waters

Corporation assumes no responsibility for any errors that may appear in this

document. This document is believed to be complete and accurate at the time

of publication. In no event shall Waters Corporation be liable for incidental or

consequential damages in connection with, or arising from, its use.

Trademarks

PIC and Waters are registered trademarks of Waters Corporation, and

Empower, e-SAT/IN, FractionLynx, MassLynx, TaperSlit, and “THE

SCIENCE OF WHAT’S POSSIBLE.” are trademarks of Waters Corporation.

Tygon is a registered trademark of Saint-Gobain Ceramics & Plastics, Inc.

Other registered trademarks or trademarks are the sole property of their

owners.

Customer comments

Waters’ Technical Communications department invites you to tell us of any

errors you encounter in this document or to suggest ideas for otherwise

improving it. Please help us better understand what you expect from our

documentation so that we can continuously improve its accuracy and

usability.

iv November 9, 2012, 715003800 Rev. A

We seriously consider every customer comment we receive. You can reach us

at [email protected].

Contact Waters

Contact Waters®with enhancement requests or technical questions regarding

the use, transportation, removal, or disposal of any Waters product. You can

reach us via the Internet, telephone, or conventional mail.

Safety considerations

Some reagents and samples used with Waters instruments and devices can

pose chemical, biological, and radiological hazards. You must know the

potentially hazardous effects of all substances you work with. Always follow

Waters contact information:

Contacting medium Information

Internet The Waters Web site includes contact

information for Waters locations worldwide.

Visit www.waters.com.

Telephone and fax From the USA or Canada, phone 800

252-HPLC, or fax 508 872 1990.

For other locations worldwide, phone and fax

numbers appear in the Waters Web site.

Conventional mail Waters Corporation

34 Maple Street

Milford, MA 01757

USA

November 9, 2012, 715003800 Rev. A v

Good Laboratory Practice, and consult your organization’s safety

representative for guidance.

When you develop methods, follow the “Protocol for the Adoption of Analytical

Methods in the Clinical Chemistry Laboratory,” American Journal of Medical

Technology, 44, 1, pages 30–37 (1978). This protocol addresses good operating

procedures and the techniques necessary to validate system and method

performance.

Safety advisories

Consult Appendix A for a comprehensive list of warning and caution

advisories.

FCC radiation emissions notice

Changes or modifications not expressly approved by the party responsible for

compliance, could void the users authority to operate the equipment. This

device complies with Part 15 of the FCC Rules. Operation is subject to the

following two conditions: (1) this device may not cause harmful interference,

and (2) this device must accept any interference received, including

interference that may cause undesired operation.

Canada spectrum management emissions notice

This class A digital product apparatus complies with Canadian ICES-003.

Cet appareil numérique de la classe A est conforme à la norme NMB-003.

Electrical power safety notice

Do not position the instrument so that it is difficult to operate the

disconnecting device.

Safety hazard symbol notice

Documentation needs to be consulted in all cases where the symbol is

used to find out the nature of the potential hazard and any actions which have

to be taken.

vi November 9, 2012, 715003800 Rev. A

Equipment misuse notice

If the equipment is used in a manner not specified by the manufacturer, the

protection provided by the equipment may be impaired.

Operate this instrument

When operating this instrument, follow standard quality-control (QC)

procedures and the guidelines presented in this section.

November 9, 2012, 715003800 Rev. A vii

Applicable symbols

Audience and purpose

This guide is intended for personnel who install, operate, and maintain 2489

UV/Visible detectors.

Intended use of the 2489 UV/Visible detector

Waters designed the 2489 UV/Visible detector to analyze and monitor many

compounds.

Symbol Definition

Manufacturer

Authorized representative of the European

Community

Confirms that a manufactured product complies

with all applicable European Community

directives

Australia C-Tick EMC compliant

Confirms that a manufactured product complies

with all applicable United States and Canadian

safety requirements

Consult instructions for use

Electrical and electronic equipment with this

symbol may contain hazardous substances and

should not be disposed of as general waste.

For compliance with the Waste Electrical and

Electronic Equipment Directive (WEEE)

2012/19/EU, contact Waters Corporation for the

correct disposal and recycling instructions.

viii November 9, 2012, 715003800 Rev. A

Calibrate

To calibrate LC systems, follow acceptable calibration methods using at least

five standards to generate a standard curve. The concentration range for

standards should include the entire range of QC samples, typical specimens,

and atypical specimens.

When calibrating mass spectrometers, consult the calibration section of the

operator’s guide for the instrument you are calibrating. In cases where an

overview and maintenance guide, not operator’s guide, accompanies the

instrument, consult the instrument’s online Help system for calibration

instructions.

Quality-control

Routinely run three QC samples that represent subnormal, normal, and

above-normal levels of a compound. Ensure that QC sample results fall within

an acceptable range, and evaluate precision from day to day and run to run.

Data collected when QC samples are out of range might not be valid. Do not

report these data until you are certain that the instrument performs

satisfactorily.

ISM classification

ISM Classification: ISM Group 1 Class B

This classification has been assigned in accordance with CISPR 11 Industrial

Scientific and Medical (ISM) instruments requirements. Group 1 products

apply to intentionally generated and/or used conductively coupled

radio-frequency energy that is necessary for the internal functioning of the

equipment. Class B products are suitable for use in both commercial and

residential locations and can be directly connected to a low voltage,

power-supply network.

November 9, 2012, 715003800 Rev. A ix

EC Authorized Representative

Waters Corporation (Micromass UK Ltd.)

Floats Road

Wythenshawe

Manchester M23 9LZ

United Kingdom

Telephone: +44-161-946-2400

Fax: +44-161-946-2480

Contact: Quality manager

x November 9, 2012, 715003800 Rev. A

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