Agilent Technologies 1260 Infinity Manuale utente

Agilent Technologies

Agilent 1260 Infinity

Purification Solution

Operator's Quick

Reference Guide

Purification Solution - Operator's Guide

Notices

No part of this manual may be reproduced

in any form or by any means (including

electronic storage and retrieval or transla-

tion into a foreign language) without prior

agreement and written consent from Agi-

lent Technologies, Inc. as governed by

United States and international copyright

laws.

Manual Part Number

M8368-90000 Rev. C

Edition

11/2016

Printed in Germany

Agilent Technologies

Hewlett-Packard-Strasse 8

76337 Waldbronn

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ject to being changed, without notice,

in future editions. Further, to the max-

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Safety Notices

CAUTION

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hazard. It calls attention to an

operating procedure, practice, or

the like that, if not correctly per-

formed or adhered to, could

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met.

WARNING

A WARNING notice denotes a

hazard. It calls attention to an

operating procedure, practice,

or the like that, if not correctly

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death. Do not proceed beyond a

WARNING notice until the indi-

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Purification Solution - Operator's Guide 3

In This Book

This manual contains instructions for Operators on how to use the Automated

Purification software.

1 The Role of the Operator

This chapter describes the role of the user of the Automated Purification

Software in Easy Prep mode.

2 Executing a Purification Task

This chapter gives instructions for users with Easy Prep user rights on how to

execute a Purification Task.

4 Purification Solution - Operator's Guide

Contents

1 The Role of the Operator 5

2 Executing a Purification Task 7

Configuring a new task 8

Running a task 9

Processing partial data and cloning tasks 10

Reviewing the analytical results 12

Reviewing the purification results 13

Forcing the Purification Task to Close 14

Purification Solution - Operator's Guide

Agilent Technologies

The Role of the Operator

This chapter describes the role of the user of the Automated Purification

Software in Easy Prep mode.

Operators of the Automated Purification Software work in Easy Prep mode to

perform purification runs. If the samples have not been analyzed on a separate

analytical instrument, the purification runs include the analytical runs.

Typically, the Operators have been trained by a Method Developer on the work

environment and operating procedures.

The Operators:

•provide the samples for use in the purification run

•know the analytical and preparative instrument and operating system

settings that apply to their samples. These have been set up in the

purification software by a Method Developer.

•set up a purification run in the purification software by creating a

purification task based on a matching task template prepared by a Method

Developer. Operators can also create a new task as a clone of an existing

task, for example, to re-run a stopped task, to run samples of the original

task that have not yet been run, or to repeat a system suitability task.

•complete the missing information (analytical data source,

analytical-to-preparative vial mapping, and injection volume) to run the

task.

•run the purification task and review the results.

For Operators, access to the Automated Purification Software is limited to the

main purification workflow user interface (the purification task screen) and

its key settings (system definition, analytical and preparative run). While in

the purification task screen, ChemStation functionality is inaccessible to avoid

unintended interference. The ChemStation screen reports are thus not

suitable for Operators.

If Operators get stuck or need help, they should contact a Method Developer.

6 Purification Solution - Operator's Guide

The Role of the Operator

In This Book

Purification Solution - Operator's Guide

Agilent Technologies

Executing a Purification Task

Configuring a new task 8

Running a task 9

Processing partial data and cloning tasks 10

Reviewing the analytical results 12

Reviewing the purification results 13

Forcing the Purification Task to Close 14

This chapter gives instructions for users with Easy Prep user rights on how to

execute a Purification Task.

A purification task in the Automated Purification Software describes all

settings of a purification experiment, including its results, once the

experiment is complete.

Purification tasks are managed in the purification task screen, which is thus

the center for the execution of all purification work:

•configuration of the purification job (samples and operating setup)

•submission of the analytical and preparative runs (including progress

indication and stop)

•review and modification of the target identification from the analytical run

•review and export of the purification results (collected fractions)

1In the ChemStation Method and Run Control view, open the Purification menu

and select Tasks.

The purification task screen is shown, displaying in its upper region a list of

all tasks in the selected task folder. Details of the selected task (setup and

result info) are displayed in the lower region. By default, the configuration

tab with the Select System page of the selected task is displayed.

8 Purification Solution - Operator's Guide

Executing a Purification Task

Configuring a new task

1Click Add Task to select a task template or choose to clone the currently

selected task and create a new purification task.

2Verify that the Analytical System matches the system providing the analytical

run, and the Preparative System that corresponds to the current preparative

instrument configuration.

The current instrument configuration of the preparative system is

displayed in the table.

If one of the systems does not match, choose another template to create

your task. If there is no matching template, ask the Method Developer to

create one.

3Select the Analytical Run page and specify the source of the analytical run:

•To run an analytical scouting sequence on a combined analytical and

preparative system, select an existing ChemStation sequence or prepare

a sequence from scratch. Select the path and name of an existing

ChemStation sequence in the text input field or click Edit sequence to

start editing a sequence from scratch or modifying a selected sequence.

You can also import a sequence as a text (CSV or TXT) file.

•To use analytical results that have already been acquired on a

stand-alone system, specify the location of the source files (result set or

single sample results) in the text input field.

Select data files using the Process? checkbox in the sequence table.

4Select the Preparative Run page and specify a Preparative base method.

5Set the preparative vial locations and injection volumes. You may want to

apply a location mapping for vial locations. Specify the injection volume for

the first sample and use the fill-down option to apply the same volume for

other samples.

Purification Solution - Operator's Guide 9

Executing a Purification Task

Running a task

1Click Run in the top toolbar of the Task dialog box to start the

purification run.

If the analytical sequence has not yet been run, it will be submitted to the

ChemStation run queue.

If the analytical run is already available, the process will start with the

evaluation of the analytical results.

The buttons in the top toolbar also allow you to stop a run in progress or

unschedule a scheduled run that has not yet started. If the run queue is

paused (for example, after a stop) you can resume it using the Resume

item in the Ta sk toolbar. Note that the / items can be helpful if the run

gets stuck or lost for any reason.

The run progress is displayed in the bottom line of the Ta s k screen. Run

state events and important processing events are displayed in the Logbook

tab.

If you stopped your run, or your run was aborted (by the instrument), call

your method developer. He can create a clone of your aborted task to enable

you to process the missed samples.

10 Purification Solution - Operator's Guide

Executing a Purification Task

Processing partial data and cloning tasks

You are dealing with partial data under the following conditions:

•You stopped your analytical or preparative run, or your run was stopped

due to an instrument error (such as solvent bottles running empty). In such

a case, some of the planned samples were executed but others were missed.

•You have a large set of analytical data from a separate analytical

instrument, but you want only a subset of it to be purified in one task. For

example, your preparative plate does not have the capacity to hold all

samples.

Task cloning (or copying) means creating a new task with the same settings

and analytical data as another task. The preparative results are not cloned.

1Select the task to be cloned in the task list of your purification task dialog

box.

2create a new task as a clone of the selected task by choosing Add clone of

currently selected task in the Add Purification Task dialog box.

If you want to repeat a (previous) system suitability test run, you can clone the

system suitability test task and let it run.

If your analytical run was stopped or aborted, the successfully processed

samples remain as analytical result data with the task. Purification proceeds

with those samples only.

1If you want to process the remaining/missed samples from such a task,

create a clone of the incomplete task.

2In the new cloned task, go to the Analytical Run page of the Task Configuration

and click Revert to sequence.

This restores your original sequence.

3Click Edit Sequence and remove the samples that already ran successfully.

4Click Accept changes to save your modified sequence with the task.

If your preparative run was stopped or aborted, the successfully processed

samples are saved with the task, which is now in a completed state, and no

further modifications can be made. You can proceed to reviewing your

purification results and export fractions as described in “Reviewing the

purification results” on page 13.

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