Agilent Technologies 1290 Infinity II 2D-LC Solution Manuale utente

Agilent 1290 Infinity II 2D-LC Solution

User Guide

2D-LC - User Guide

Notices

Document Information

Document No: SD-29000383 Rev. B

Part No: G2198-90001 Rev. B

EDITION 02/2020

No part of this manual may be repro-

duced in any form or by any means

(including electronic storage and retrieval

or translation into a foreign language)

without prior agreement and written con-

sent from Agilent Technologies, Inc. as

governed by United States and interna-

tional copyright laws.

Agilent Technologies

Hewlett-Packard-Strasse 8

76337 Waldbronn

Warranty

The material contained in this document

is provided “as is,” and is subject to being

changed, without notice, in future edi-

tions. Further, to the maximum extent

permitted by applicable law, Agilent dis-

claims all warranties, either express or

implied, with regard to this manual and

any information contained herein, includ-

ing but not limited to the implied warran-

ties of merchantability and fitness for a

particular purpose. Agilent shall not be lia-

ble for errors or for incidental or conse-

quential damages in connection with the

furnishing, use, or performance of this

document or of any information con-

tained herein. Should Agilent and the user

have a separate written agreement with

warranty terms covering the material in

this document that conflict with these

terms, the warranty terms in the separate

agreement shall control.

Technology Licenses

The hardware and/or software described

in this document are furnished under a

license and may be used or copied only in

accordance with the terms of such

license.

Restricted Rights Legend

U.S. Government Restricted Rights. Soft-

ware and technical data rights granted to

the federal government include only

those rights customarily provided to end

user customers. Agilent provides this

customary commercial license in Soft-

ware and technical data pursuant to FAR

12.211 (Technical Data) and 12.212

(Computer Software) and, for the Depart-

ment of Defense, DFARS 252.227-7015

(Technical Data - Commercial Items) and

DFARS 227.7202-3 (Rights in Commer-

cial Computer Software or Computer

Software Documentation).

Safety Notices

CAUTION

notice denotes a

hazard. It calls attention to an

operating procedure, practice, or

the like that, if not correctly per-

formed or adhered to, could result

in damage to the product or loss

of important data. Do not proceed

beyond a

CAUTION

notice until

the indicated conditions are fully

understood and met.

WARNING

A WARNING notice denotes a

hazard. It calls attention to an

operating procedure, practice, or

the like that, if not correctly per-

formed or adhered to, could

result in personal injury or death.

Do not proceed beyond a WARN-

ING notice until the indicated

conditions are fully understood

and met.

2D-LC - User Guide 3

In This Book

This manual covers the Agilent 1290 Infinity II 2D-LC Solution and 2D-LC

Acquisition software (G2198AA).

1Introduction

This chapter describes the product of Agilent 1290 Infinity II 2D-LC Solution.

2Conceptsof2D-LC

This chapter describes the concepts of Agilent 1290 Infinity II 2D-LC Solution.

3 Compatibility Matrix

The compatibility matrix provides information about installation and execution

prerequisites with respect to hardware, firmware and the operating system. Agilent

2D-LC Software is an OpenLAB CDS ChemStation Edition plug-in.

4Installation

This chapter describes the installation of the Agilent 1290 Infinity II 2D-LC Solution.

These installation instructions are valid for the modes standard heart-cutting,

multiple heart-cutting, high resolution sampling and comprehensive 2D-LC except

the connections to the 2D-LC valve. These connections depend on the method, see

chapter "Run the System for detailed information.

5 Method Parameters

This chapter provides background information on method parameters. It helps to

optimize methods in Agilent 1290 Infinity II 2D-LC Solution in the modes standard

heart-cutting, multiple heart-cutting, high resolution sampling and comprehensive

2D-LC.

6 Run the System

This chapter describes how to run the Agilent 1290 Infinity II 2D-LC Solution in the

modes standard heart-cutting, multiple heart-cutting, high resolution sampling and

comprehensive 2D-LC.

2D-LC - User Guide 4

7 Investigate the effects of using different gradients in the 2Dimension

This chapter describes, how shifted gradients in the second dimension can be

used to enlarge the accessible two-dimensional separation space.

8 Data Analysis

This chapter descibes the analyzation of data in 2D-LC and is separated in a

section heart-cutting 2D-LC and a section comprehensive 2D-LC.

9 Troubleshooting and Diagnostics

This chapter gives an overview about the troubleshooting and diagnostic features

and the different user interfaces.

10 Error Information

This chapter describes the meaning of error messages, and provides information

on probable causes and suggested actions how to recover from error conditions.

11 Maintenance

This chapter describes the maintenance of the 2D-LC Solution.

12 Parts for Maintenance

This chapter provides information on parts material required for the solution.

13 Alternative ways to install the System

This chapter describes alternative ways to install and setup the system.

14 Agilent 2D-LC Solution with the selectivity of mass selective detection

(MSD)

This chapter describes the different options to use the Agilent 2D-LC Solution with

mass selective detection (MSD).

2D-LC - User Guide 5

15 Theoretical Background

This chapter gives the theoratical background of 2D-LC and describes the system

components (soft- and hardware) of the Agilent 1290 Infinity II 2D-LC Solution.

16 Appendix

This chapter provides addition information on safety, legal and web.

2D-LC - User Guide 6

Contents

1Introduction10

Product Description 11

Features 13

2Conceptsof2D-LC14

Concepts of 2D-LC 15

Heart-Cutting 2D-LC (LC-LC) 16

Multiple Heart-Cutting and High Resolution Sampling 2D-LC 17

Comprehensive 2D-LC (LCxLC) 29

Triggering of 2D-LC 31

Active solvent Modulation (ASM) 35

3 Compatibility Matrix 45

Supported Chromatographic Data Systems 46

Supported Drivers 47

Supported Operating Systems 48

Supported Firmware 49

Available Languages 50

PC Requirements 51

4Installation52

Delivery checklist 53

Options 54

Recommendations for instrument setup 58

Installation of the 2D-Valves 75

2D-LC Software Configuration 84

Installation of Active Solvent Modulation (ASM) 86

2D-LC - User Guide 7

5 Method Parameters 87

Method Parameters Standard Heart-Cutting 2D-LC 88

Method Parameters Multiple Heart-Cutting and High Resolution Sampling

2D-LC 104

Method Parameters Comprehensive 2D-LC 127

Method Development of Active Solvent Modulation (ASM) 141

6 Run the System 146

Connect the capillaries to the 2D-LC valve 147

Standard Heart-Cutting 2D-LC 148

Multiple Heart-Cutting and High Resolution Sampling 2D-LC 159

Comprehensive 2D-LC 175

Active Solvent Modulation (ASM) 189

7 Investigate the effects of using different gradients in the

2Dimension 192

Investigate the effects of using different gradients in the 2Dimension 193

8 Data Analysis 200

Data Analysis for Heartcutting 2D-LC (LC-LC) 201

Data Analysis for Comprehensive 2D-LC (LCxLC) 219

9Troubleshootingand Diagnostics 231

Overview of the Module’s Indicators and Test Functions 232

User Interfaces 233

Agilent Lab Advisor Software 234

The Basic Principle of Troubleshooting 235

Recommended Tests to Conclude Troubleshooting 245

10 Error Information 246

What Are Error Messages 247

General Error Messages 248

Module-Specific Error Messages 254

2D-LC - User Guide 8

11 Maintenance 258

Introduction to Maintenance 259

Warnings and Cautions 260

Overview of Maintenance 262

Cleaning the Module 263

Correcting Leaks 264

Replace Valve Heads 266

Replacing Parts of the Valve Head 272

Replacing the Fuses of the Infinity Valve Drive 274

Replace the Module Firmware 276

12 Parts for Maintenance 277

2D-LC Loops 278

2D-LC Capillaries 279

ASM Capillaries 280

Pressure Release Kit 281

2D-LC Easy Starter Kit 282

Valve Drive Parts 283

Valve Driver Parts Infinity II 284

Valve Head Parts 285

Vale Options Overview (for 2D-LC) 287

MS Diverter Valve 291

Valve Options Overview (for G7116B) 293

Additional Heater Devices 295

Accessories and Consumables (for G7116B) 297

InfinityLab Quick Connect and Quick Turn Fittings 299

Capillary Kits 303

Important Customer Web Links 304

13 Alternative ways to install the System 305

Legacy Stack Configuration 306

2D-LC - User Guide 9

14 Agilent 2D-LC Solution with the selectivity of mass selective detection

(MSD) 311

Agilent 2D-LC Solution with an Agilent Single Quadrupole Detector 312

Agilent 2D-LC Solution with high-end MS detection 318

15 Theoretical Background 322

Theoretical basis of 2D-LC 323

2nd dimension as detector 331

Successful Mode Combinations 333

Solvent Elution Modes 334

Practical Issues 340

16 Appendix 342

General Safety Information 343

Waste Electrical and Electronic Equipment (WEEE) Directive 350

Radio Interference 351

Sound Emission 352

Solvent Information 353

Further Information 354

Agilent Technologies on Internet 355

2D-LC - User Guide 10

1Introduction

Product Description 11

Features 13

This chapter describes the product of Agilent 1290 Infinity II 2D-LC Solution.

Altri manuali per 1290 Infinity II 2D-LC Solution

Questo manuale è adatto per i seguenti modelli

Indice

Altri manuali Agilent Technologies Attrezzature da laboratorio

Agilent Technologies

Agilent Technologies MX3000P Guida di sicurezza

Agilent Technologies

Agilent Technologies OmniBER 720 Manuale utente

Agilent Technologies

Agilent Technologies 1260 Infinity VL Manuale utente

Agilent Technologies

Agilent Technologies TS-5400 Manuale utente

Agilent Technologies

Agilent Technologies 8511B Manuale di servizio

Agilent Technologies

Agilent Technologies 5800 ICP-OES Manuale utente

Agilent Technologies

Agilent Technologies G1888 Manuale operativo

Agilent Technologies

Agilent Technologies MP-AES Guida

Agilent Technologies

Agilent Technologies Magnis NGS Prep System Manuale utente

Agilent Technologies

Agilent Technologies 1260 Infinity Manuale utente

Agilent Technologies

Agilent Technologies 1100 Series Guida utente

Agilent Technologies

Agilent Technologies 7200 Manuale utente

Agilent Technologies

Agilent Technologies Turbo-V 6000 Manuale utente

Agilent Technologies

Agilent Technologies 5110 ICP-OES Manuale utente

Agilent Technologies

Agilent Technologies 1260Infinity II Bio-inert FractionCollector Manuale utente

Agilent Technologies

Agilent Technologies BenchCel Microplate Handler Manuale utente

Agilent Technologies

Agilent Technologies PlateLoc Manuale utente

Agilent Technologies

Agilent Technologies DNA 1000 Kit Manuale utente

Agilent Technologies

Agilent Technologies UHV-24 Manuale utente

Agilent Technologies

Agilent Technologies 1260 Infinity Manuale utente

Agilent Technologies

Agilent Technologies 7694 Manuale utente

Agilent Technologies

Agilent Technologies 75000 Series C Manuale utente

Agilent Technologies

Agilent Technologies 7890A Manuale utente

Agilent Technologies

Agilent Technologies TS-8900 Istruzioni operative

Manuali Attrezzature da laboratorio popolari di altre marche

Qiagen

Qiagen DML 3000 Manuale utente

Queensgate

Queensgate NANOSCAN OP400 Manuale di programmazione

Conviron

Conviron GEN1000-GE Manuale utente

Parr Instrument

Parr Instrument 4560 Manuale di istruzioni

IKA

IKA IC control Manuale utente

Integra

Integra DOSE IT Manuale utente

Endress+Hauser

Endress+Hauser Cleanfit CPA875 Manuale utente

NI PXI-5422 Manuale

Collomix

Collomix Aqix Manuale utente

SPEX SamplePrep

SPEX SamplePrep 6875 Freezer/Mill Series Manuale utente

Ocean Insight

Ocean Insight FLAME-NIR+ Manuale utente

Parker

Parker ALIGN-MG-NA Manuale utente

BD 644787 Manuale utente

DENTAURUM

DENTAURUM Compact Megaplus Manuale utente

Biuged Laboratory Instruments

Biuged Laboratory Instruments BGD 626 Manuale utente

VWR

VWR SAS Super IAQ Manuale utente

illumina

illumina MiSeqDx Manuale utente

Yamato

Yamato BM510 Manuale utente