Agilent Technologies InfinityLab LC Series Manuale utente

Agilent Technologies

Agilent InfinityLab LC Series

1260 Infinity II Binary LC

System

Manual and Quick Guide

1260 Infinity II Binary LC System - Manual and Quick Guide

Notices

No part of this manual may be reproduced

in any form or by any means (including

electronic storage and retrieval or transla-

tion into a foreign language) without prior

agreement and written consent from Agi-

lent Technologies, Inc. as governed by

United States and international copyright

laws.

Manual Part Number

G7112-90300

Edition

09/2016

Printed in Germany

Agilent Technologies

Hewlett-Packard-Strasse 8

76337 Waldbronn

Warranty

The material contained in this docu-

ment is provided “as is,” and is sub-

ject to being changed, without notice,

in future editions. Further, to the max-

imum extent permitted by applicable

law, Agilent disclaims all warranties,

either express or implied, with regard

to this manual and any information

contained herein, including but not

limited to the implied warranties of

merchantability and fitness for a par-

ticular purpose. Agilent shall not be

liable for errors or for incidental or

consequential damages in connection

with the furnishing, use, or perfor-

mance of this document or of any

information contained herein. Should

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written agreement with warranty

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arate agreement shall control.

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this document are furnished under a

license and may be used or copied only in

accordance with the terms of such license.

Restricted Rights Legend

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contract, Software is delivered and licensed

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(June 1987) or any equivalent agency regu-

lation or contract clause. Use, duplication

or disclosure of Software is subject to Agi-

lent Technologies’ standard commercial

license terms, and non-DOD Departments

and Agencies of the U.S. Government will

receive no greater than Restricted Rights as

defined in FAR 52.227-19(c)(1-2) (June

1987). U.S. Government users will receive

no greater than Limited Rights as defined in

FAR 52.227-14 (June 1987) or DFAR

252.227-7015 (b)(2) (November 1995), as

applicable in any technical data.

Safety Notices

CAUTION

A CAUTION notice denotes a

hazard. It calls attention to an

operating procedure, practice, or

the like that, if not correctly per-

formed or adhered to, could

result in damage to the product

or loss of important data. Do not

proceed beyond a CAUTION

notice until the indicated condi-

tions are fully understood and

met.

WARNING

A WARNING notice denotes a

hazard. It calls attention to an

operating procedure, practice,

or the like that, if not correctly

performed or adhered to, could

result in personal injury or

death. Do not proceed beyond a

WARNING notice until the indi-

cated conditions are fully

understood and met.

1260 Infinity II Binary LC System - Manual and Quick Guide 3

In This Book

This book describes the Agilent InfinityLab LC Series 1260 Infinity II Binary

LC system.

1Introduction

This chapter gives an introduction to the Agilent 1260 Infinity II LC, the

underlying concepts and the features of the Agilent 1260 Infinity II LC.

2 Configuration Settings

This chapter describes how to configure the system.

3QuickStartGuide

This chapter provides information on running an Agilent 1260 Infinity II LC.

4 Parts and Consumables

This chapter provides information on additional parts and consumables.

5 Appendix

This chapter provides addition information on safety, legal, and web.

4 1260 Infinity II Binary LC System - Manual and Quick Guide

Contents

1Introduction 5

Product Description 6

Features of the Agilent 1260 Infinity II Binary LC 7

System Components 8

Solutions 18

Optimizing the Stack Configuration 22

Leak and Waste Handling 28

2 Configuration Settings 35

General Information on LAN Configuration 36

Instrument Configuration 37

Lab Advisor 39

3QuickStartGuide 45

Best Practices 46

Prepare a Run 47

Check Out the System 54

4 Parts and Consumables 65

Tool Kit 66

InfinityLab Quick Connect and Quick Turn Fittings 68

5 Appendix 73

Safety 74

Agilent Technologies on Internet 80

1260 Infinity II Binary LC System - Manual and Quick Guide

Agilent Technologies

Introduction

Product Description 6

Features of the Agilent 1260 Infinity II Binary LC 7

System Components 8

Binary Pump G7112B 9

Multisampler G7167A 10

Vialsampler G7129A 11

Multicolumn Thermostat G7116A 12

Diode Array Detector HS G7117C 13

Diode Array Detector WR G7115A 14

Variable Wavelength Detector G7114A 15

Fluorescence Detector G7121A 16

Refractive Index Detector G7162A 17

Solutions 18

Walk-up 18

Online SPE 18

1260 Infinity II Multimethod Solution 20

Optimizing the Stack Configuration 22

Agilent InfinityLab LC Flex Bench 22

One Stack Configuration 24

Two Stack Configuration 26

Leak and Waste Handling 28

Waste Guidance 32

Waste Concept 33

This chapter gives an introduction to the Agilent 1260 Infinity II LC, the

underlying concepts and the features of the Agilent 1260 Infinity II LC.

6 1260 Infinity II Binary LC System - Manual and Quick Guide

Introduction

Product Description

The Agilent 1260 Infinity II LC System is the trusted platform, taking you to

the next level of routine analysis, and giving you the instrument choice to

achieve best operational efficiency.

A broad range of reliable instrumentation matches with latest column

technologies and guarantees robust separation and detection performance.

Highest instrument utilization and a fast turnaround cycle is achieved through

easy column handling and superior sample logistics from sample submission

to data analysis.

The designed for method transferability and stepwise upgrade capability

enables a risk-free integration in current infrastructure matching your budget.

1260 Infinity II Binary LC System - Manual and Quick Guide 7

Introduction

Features of the Agilent 1260 Infinity II Binary LC

With an operating pressure of up to 600 bar, the 1260 Infinity II Binary Pump

is compatible with HPLC and UHPLC, i.e. handling 2.1, 3, and 4.6 mm ID

columns over the flow rate range (up to 5 mL/min). Gradient formation is

based on a high-pressure mixing principle.

Ultralow carryover – The 1260 Infinity II Multisampler is designed for low

carryover, you can take clean to a whole new level with the multi-wash

capability, cleaning all relevant injection parts between runs. This

sophisticated, integrated feature flushes the injection needle outside with

three solvents, and uses seat back flush procedures to reduce carryover to less

than 9 ppm.

Dual-needle injection – By running samples alternately through one or the

other injection path, you can reduce cycle times to mere seconds, virtually

eliminating conventional wait times—whether for large volume loadings or

flushing procedures.

Higher sample capacity per benchspace – Using shallow well-plate drawers,

the 1260 Infinity II Multisampler takes a maximum load of 16 microtiter plates

and up to 6144 samples—the most of any single system.

Advanced column capacity for up to 4 columns in a single 1260 Infinity II

Multicolumn Thermostat delivering best flexibility for column switching.

Fast and easy connections with InfinityLab Quick Connect fittings to save time

and trouble.

1260 Infinity II DAD HS Detector delivers lower detection limit and higher

data quality for more confidence.

Seamless integration in your chromatography data system: Agilent’s

Instrument Control Framework (ICF) enables smooth control of Agilent LC

instrumentation through third-party chromatography data systems.

8 1260 Infinity II Binary LC System - Manual and Quick Guide

Introduction

System Components

The Agilent 1260 Infinity II Binary LC system consists of the following

components:

•Binary Pump

•Multisampler or Vialsampler

•Multicolumn Thermostat (MCT)

•Diode Array (DAD), Variable Wavelength (VWD), Refractive Index (RID), or

Fluorescence Detector (FLD)

•Evaporative Light Scattering Detector (ELSD) (optional, not stackable)

•Solvent Cabinet

The Agilent 1260 Infinity II Binary LC is described in more detail in the

following sections. All modules (except the optional ELSD) are stackable, see

“Optimizing the Stack Configuration” on page 22.

For specifications, please refer to the individual module user documentation.

1260 Infinity II Binary LC System - Manual and Quick Guide 9

Introduction

System Components

Binary Pump G7112B

The Agilent 1260 Infinity II Binary Pump is suited for LC applications using

conventional or superficially porous particles columns, as well as STM column

technology. Gradient formation is based on a high-pressure mixing principle.

Standard or low delay volumes can be easily configured by the user. The

Agilent 1260 Infinity II Binary Pump offers reproducible gradients and

high-performance, providing high-throughput and fast separations. The pump

is suitable for routine applications, with UV or MS detection, where high speed

and resolution with uncompromised data quality are required.

Figure 1 Overview of the pump

Status indicator

Active seal wash

Purge valve

Degasser

Pump head A

Pump head B

Leak drain

Power switch

10 1260 Infinity II Binary LC System - Manual and Quick Guide

Introduction

System Components

Multisampler G7167A

The Agilent 1260 Infinity II Multisampler can handle both vials and microtiter

plates with ease and efficiency up to 600 bar system pressure, optimized on

high flexibility.

This compact module can house up to 6144 samples, all inside the Agilent

stack footprint and the robotics to inject each into the chromatograph in turn.

With the multi-wash capability, you can reduce carryover to less than 9 parts

per million.

Figure 2 Overview of the Multisampler

6WDWXVLQGLFDWRU

1HHGOHDVVHPEO\

+RWHOGUDZHU

/HDNGUDLQ

3RZHUVZLWFK

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