Agilent Technologies 2100 Bioanalyzer System Manuale utente

Agilent 2100 Bioanalyzer

2100 Expert User’s Guide

Contents ▲2▼Index

Notices

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translation into a foreign language) without prior agreement

and written consent from Agilent Technologies, Inc. as

governed by United States and international copyright laws.

Manual Part number: G2946-90004

Edition: May 2005

Agilent Technologies

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Germany

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The hardware and/or software described in this document

are furnished under a license and may be used or copied

only in accordance with the terms of such license.

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and is subject to being changed, without notice, in future

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to Agilent Technologies’ standard commercial license

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Government will receive no greater than Restricted Rights

as defined in FAR 52.227-19(c)(1-2) (June 1987). U.S. Gov-

ernment users will receive no greater than Limited Rights as

defined in FAR 52.227-14 (June 1987) or DFAR 252.227-7015

(b)(2) (November 1995), as applicable in any technical data.

Contents ▲3▼Index

Contents

About this Manual ..................................................................................................................5

In this Manual............................................................................................................................. 6

Related Documents ................................................................................................................... 8

How to Use this Manual ......................................................................................................... 10

Quick Start.............................................................................................................................14

Looking at 2100 Expert .........................................................................................................28

Introduction to the Key Features of the 2100 expert.......................................................... 29

Starting 2100 Expert ................................................................................................................ 31

2100 Expert Work Area ........................................................................................................... 32

Closing 2100 Expert ................................................................................................................. 43

Running and Evaluating Electrophoretic Assays ..............................................................45

Principles of Nucleic Acid and Protein Analysis on a Chip............................................... 46

Preparing and Running an Electrophoretic Assay.............................................................. 50

Analyzing and Evaluating the Results of an Electrophoretic Assay................................ 81

Result Flagging....................................................................................................................... 152

Running and Evaluating Flow Cytometric Assays...........................................................166

Principles of Flow Cytometric Measurements.................................................................. 167

Overview of Flow Cytometric Assays ................................................................................. 176

Contents ▲4▼Index

Preparing and Running a Flow Cytometric Assay ............................................................ 179

Analyzing and Evaluating the Results of a Flow Cytometric Assay............................... 211

Working with Chip Data and Assays................................................................................258

2100 Expert Data Overview .................................................................................................. 259

Handling Assays..................................................................................................................... 262

Handling Chip Data................................................................................................................ 267

Organizing, Backing up, and Archiving 2100 Expert Data ............................................... 269

Importing Data........................................................................................................................ 271

Exporting Data........................................................................................................................ 277

Printing Reports ..................................................................................................................... 286

Configuring Tables................................................................................................................. 293

Administering System Functions ......................................................................................299

Configuring 2100 expert........................................................................................................ 300

Using Log Books .................................................................................................................... 310

Running Instrument Diagnostics ......................................................................................316

How to Run Instrument Diagnostics Tests........................................................................ 321

Performing Verifications....................................................................................................325

Products, Spare Parts, and Accessories..........................................................................334

Glossary ...............................................................................................................................338

Contents ▲5▼Index

About this Manual

Welcome to the User’s Guide for the Agilent 2100 expert software. This manual provides

beginners and advanced users with information needed to successfully run

electrophoretic and flow cytometric assays with the bioanalyzer.

The 2100 expert software allows the control of the bioanalyzer (including diagnostic

functions) and, in combination with a LabChip kit, the acquisition, interpretation and

result presentation of data generated during the analysis of DNA, RNA, proteins, and

cells.

Contents ▲6▼Index

In this Manual

This manual provides bioanalyzer users with the following information:

•“About this Manual” on page 5 gives an overview of the subjects in this manual, and

lists major innovations and improvements of the 2100 expert software. It also lists

supplemental literature and shows you how to make efficient use of this manual.

•“Quick Start” on page 14 is meant for experienced users. It briefly summarizes the

necessary steps to prepare and run an assay.

•“Looking at 2100 Expert” on page 28 shows how to get started with the 2100 expert

software, and outlines its main operational possibilities.

•“Running and Evaluating Electrophoretic Assays” on page 45 explains how

electrophoretic measurements are made using the bioanalyzer, gives detailed

descriptions of all steps necessary to run electrophoretic assays, and shows how to

analyze and evaluate results using electropherograms and gel-like images.

•“Running and Evaluating Flow Cytometric Assays” on page 166 explains how flow

cytometric measurements are made using the bioanalyzer, gives detailed descriptions

of all steps necessary to run flow cytometric assays, and shows how to analyze and

evaluate results using histograms and dot plots.

•“Working with Chip Data and Assays” on page 258 shows you what to do to open, save,

import and export files, and how to print the results.

•“Administering System Functions” on page 299 is your guideline for configuring the

2100 expert software.

•“Running Instrument Diagnostics” on page 316 shows how to use the diagnostic tests

to check the bioanalyzer hardware for proper functioning.

Contents ▲7▼Index

•“Performing Verifications” on page 325 describes how you can validate your

bioanalyzer system.

•“Products, Spare Parts, and Accessories” on page 334 lists all parts and

accessories—including reorder numbers—that are required for electrophoretic and

flow cytometric measurements.

•“Glossary” on page 338 explains terms in context with flow cytometry, electrophoresis,

and terms specific to the bioanalyzer software and hardware.

If you have any questions this manual cannot answer, please refer to the supplemental

literature listed in “Related Documents” on page 8. If you still have questions, contact

Agilent for additional support at:

http://www.agilent.com/chem/labonachip

Contents ▲8▼Index

Related Documents

A collection of supplemental literature is given in the following.

Bioanalyzer Manuals

CD-ROM

Reagent Kit Guides

The Reagent Kit Guides give you information on how to perform specific assays,

including sample and chip preparation.

Publication Number Title

G2938-90006 Agilent 2100 Bioanalyzer Installation and Safety Manual

G2946-90003Agilent 2100 Bioanalyzer Maintenance andTroubleshooting

Guide

Publication Number Title

G2946-60002 Agilent 2100 Bioanalyzer – How to Use Multimedia CD-ROM

Publication Number Title

G2938-90300 Kit Guide Binder English (including all Reagent Kit Guides)

G2938-90010 Reagent Kit Guide DNA 500 and DNA 1000 Assay

G2938-90020 Reagent Kit Guide DNA 7500 and DNA 12000 Assay

Contents ▲9▼Index

Application Notes and Technical Notes

Application Notes and Technical Notes are available from the Agilent 2100 Bioanalyzer

Help Desk or from the lab-on-a-chip web pages:

http://www.agilent.com/chem/labonachip

Newly Published Documentation

Follow this link to see if there is any new documentation:

http://www.chem.agilent.com/scripts/Library.asp

G2938-90030 Reagent Kit Guide RNA 6000 Nano Assay

G2938-90040 Reagent Kit Guide RNA 6000 Pico Assay

G2938-90050 Reagent Kit Guide Protein 200 Plus Assay

G2938-90060 Reagent Kit Guide Protein 50 Assay

G2938-90070 Reagent Kit Guide Cell Fluorescence Assays

G2938-90080 Reagent Kit Guide Cell Fluorescence Checkout Kit

Publication Number Title

Contents ▲10 ▼Index

How to Use this Manual

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conventions.

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