Agilent Technologies 1260 Infinity Manuale utente

Agilent 1260 Infinity

Preparative Pump

User Manual

Agilent 1260 Infinity Preparative Pump User Manual

Notices

No part of this manual may be reproduced

in any form or by any means (including elec-

tronic storage and retrieval or translation

into a foreign language) without prior agree-

ment and written consent from Agilent

Technologies, Inc. as governed by United

States and international copyright laws.

Manual Part Number

G1361-90012

Edition

06/2010

Printed in Germany

Agilent Technologies

Hewlett-Packard-Strasse 8

76337 Waldbronn

Research Use Only

Not for use in Diagnostic Procedures.

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The material contained in this docu-

ment is provided “as is,” and is sub-

ject to being changed, without notice,

in future editions. Further, to the max-

imum extent permitted by applicable

law, Agilent disclaims all warranties,

either express or implied, with regard

to this manual and any information

contained herein, including but not

limited to the implied warranties of

merchantability and fitness for a par-

ticular purpose. Agilent shall not be

liable for errors or for incidental or

consequential damages in connection

with the furnishing, use, or perfor-

mance of this document or of any

information contained herein. Should

Agilent and the user have a separate

written agreement with warranty

terms covering the material in this

document that conflict with these

terms, the warranty terms in the sep-

arate agreement shall control.

Technology Licenses

The hardware and/or software described in

this document are furnished under a license

and may be used or copied only in accor-

dance with the terms of such license.

Restricted Rights Legend

If software is for use in the performance of a

U.S. Government prime contract or subcon-

tract, Software is delivered and licensed as

“Commercial computer software” as

defined in DFAR 252.227-7014 (June 1995),

or as a “commercial item” as defined in FAR

2.101(a) or as “Restricted computer soft-

ware” as defined in FAR 52.227-19 (June

1987) or any equivalent agency regulation

or contract clause. Use, duplication or dis-

closure of Software is subject to Agilent

Technologies’ standard commercial license

terms, and non-DOD Departments and

Agencies of the U.S. Government will

receive no greater than Restricted Rights as

defined in FAR 52.227-19(c)(1-2) (June

1987). U.S. Government users will receive

no greater than Limited Rights as defined in

FAR 52.227-14 (June 1987) or DFAR

252.227-7015 (b)(2) (November 1995), as

applicable in any technical data.

Safety Notices

CAUTION

A CAUTION notice denotes a

hazard. It calls attention to an

operating procedure, practice, or

the like that, if not correctly per-

formed or adhered to, could

result in damage to the product

or loss of important data. Do not

proceed beyond a CAUTION

notice until the indicated condi-

tions are fully understood and

met.

WARNING

A WARNING notice denotes a

hazard. It calls attention to an

operating procedure, practice,

or the like that, if not correctly

performed or adhered to, could

result in personal injury or

death. Do not proceed beyond a

WARNING notice until the indi-

cated conditions are fully under-

stood and met.

This product may be used as a

com-ponent of an in vitro diagnostic

sys-tem if the system is registered

with the appropriate authorities and

com-plies with the relevant reg-

ula-tions. Otherwise, it is intended

only for gen-eral laboratory use.

Agilent 1260 Infinity Preparative Pump User Manual 3

Contents

1 Introduction to the Preparative Pump 7

Introduction to the Preparative Pump 8

Instrument Layout 12

The Electronics 13

Electrical Connections 14

Interfaces 16

2 Site Requirements and Specifications 23

Site Requirements 24

Physical Specifications 27

Performance Specifications 28

3 Installing the Pump 29

Unpacking the Preparative Pump 30

Optimizing the Stack Configuration 34

Installing the Preparative Pump 36

Connecting Modules and Control Software 40

Flow Connections for a Single (Isocratic) Preparative Pump 42

Flow Connections for a Dual (Binary Gradient) Preparative Pump 45

Get the System Ready for the First Analysis 49

4 Using the Preparative Pump 51

Hints for Successful Use of the Preparative Pump 52

Solvent Information 53

Prevent Blocking of Solvent Filters 54

Algae Growth in HPLC Systems 55

Setting Up the Pump 57

4 Agilent 1260 Infinity Preparative Pump User Manual

Contents

5 Optimizing Performance 65

How to Configure the Seal Wash Function 66

How to Optimize the Compressibility Compensation Setting 67

6 Troubleshooting and Diagnostics 69

Agilent Lab Advisor software 70

Overview of the Pump’s Indicators and Test Functions 71

Status Indicators 72

User Interfaces 74

Error Messages 75

Pressure Test 94

Leak Test 98

EMPV Cleaning 103

7 Maintenance 107

Introduction to Maintenance and Repair 108

Overview of Maintenance and Repair 112

Simple Repairs 114

8 Parts and Materials for Maintenance 133

Pump Housing and Main Assemblies 134

Pump-Head Assembly 138

Solvent Cabinet and Solvent Inlet Parts 140

Hydraulic Path 142

Preparative Pump Basis Kit 145

Preparative Pump Gradient Kit 147

9 Configuring the Preparative Pump 149

Preparative Pump Main Board (PPM) 150

Optional Interface Boards 155

Interfaces 159

Main Power Supply Assembly 164

Agilent 1260 Infinity Preparative Pump User Manual 5

Contents

10 Appendix 167

General Safety Information 168

The Waste Electrical and Electronic Equipment (WEEE) Directive

(2002/96/EC) 172

Lithium Batteries Information 173

Radio Interference 174

Sound Emission 175

Solvent Information 176

Agilent Technologies on Internet 178

6 Agilent 1260 Infinity Preparative Pump User Manual

Contents

Agilent 1260 Infinity Preparative Pump User Manual

Agilent Technologies

Introduction to the Preparative Pump

Introduction to the Preparative Pump 8

Hydraulic Path Overview 9

How Does Compressibility Compensation Work? 10

Early Maintenance Feedback (EMF) 11

Instrument Layout 12

The Electronics 13

Electrical Connections 14

Interfaces 16

8 Agilent 1260 Infinity Preparative Pump User Manual

1Introduction to the Preparative Pump

Introduction to the Preparative Pump

The preparative pump is a cam driven pump, with fixed stroke and two

parallel pistons, each with (identical ball and seat) inlet and outlet valve. Each

of the two pump pistons is capable of delivering a maximum of 50 ml/min flow

at up to 400 bar pressure.

The two cams have overlapping delivery cycles. Compression losses are

calculated and smooth delivery is ensured through variation of the motor

speed over the pump cycle.

The pistons are guided through two seals each. The gap between those seals is

connected to capillary fittings, this way seal wash is not only an option, but

integral part of every pump. A peristaltic pump, to automate seal wash, is also

part of the system.

Figure 1 Overview of the Preparative Pump

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Agilent 1260 Infinity Preparative Pump User Manual 9

Introduction to the Preparative Pump 1

Introduction to the Preparative Pump

Hydraulic Path Overview

The pump flow leaving the pump head passes through the multi-assembly,

which combines the flow of the two pistons, filters the solvent and contains a

pressure sensor to monitor system pressure. No hydraulic damper is included

in the system. This makes very fast gradients possible. From the

multi-assembly the flow is routed to the purge valve and from there either

directly into waste or on to the next module (normally injection valve or

sampling device).

Gradients are formed with two combined pumps, as high pressure gradients.

The combined flow, is flowing through a passive mixer, to smooth composition.

With its high pressure mixing principle gradient systems and the usually high

flow rates, no degassing of solvents is necessary.

To avoid outgassing in the detector cell a back pressure regulating device is

recommended for applications that demand it.

Figure 2 Schematics of the Preparative Pump

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10 Agilent 1260 Infinity Preparative Pump User Manual

1Introduction to the Preparative Pump

Introduction to the Preparative Pump

For pump specifications, see “Site Requirements” on page 24.

How Does Compressibility Compensation Work?

The compressibility of the solvents in use will affect retention-time stability

when the back pressure in the system changes (for example, ageing of

column). In order to minimize this effect, the pump provides a compressibility

compensation feature which optimizes the flow stability according to the

solvent type. The compressibility compensation is set to a default value and

can be changed through the user interface.

Without a compressibility compensation the following will happen during a

stroke of the first piston. The pressure in the piston chamber increases and

the volume in the chamber will be compressed depending on backpressure and

solvent type. The volume displaced into the system will be reduced by the

compressed volume.

When a compressibility compensation value for the pump head is set, the

pump processor calculates a compensation volume that depends on the

system pressure and the selected compressibility value. The pump has a fixed

stroke. To compensate for compressibility losses, the speed of the piston

movement has to be varied during the different parts of each stroke.

Table 1 Preparative Pump Details

Materials in contact with mobile phase

Bottle head assembly glass frits, PTFE compounds, PEEK

Pump head SST, sapphire

Inlet/Outlet Valves SST, sapphire, ruby, PEEK

EMPV SST, ruby, sapphire, PEEK

Filter Cup SST

Filter Plate SST, PEEK

Pressure Sensor SST, PEEK

Capillaries SST

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