BD FACSymphony A1 Flow Manuale utente
FACSymphony™A1 Flow
Cytometer
User's Guide
23-23437(01)
2022-07 For Research Use Only
Copyrights
No part of this publication may be reproduced, transmitted, transcribed, stored in retrieval systems, or
translated into any language or computer language, in any form or by any means: electronic, mechanical,
magnetic, optical, chemical, manual, or otherwise, without prior written permission from BD.
The information in this guide is subject to change without notice. BD reserves the right to change its products
and services at any time. Although this guide has been prepared with every precaution to ensure accuracy, BD
assumes no liability for any errors or omissions, nor for any damages resulting from the application or use of
this information. BD welcomes customer input on corrections and suggestions for improvement.
Trademarks
BD, the BD Logo, BD FACSClean, BD FACSDiva, BD FACSFlow, BD FACSymphony, BD Horizon Brilliant, FACS and
Horizon are trademarks of Becton, Dickinson and Company or its affiliates. All other trademarks are the
property of their respective owners. ©2022 BD. All rights reserved.
Alexa Fluor™ and Pacific Blue™ are trademarks of Life Technologies Corporation.
Cy™ is a trademark of GE Healthcare. Cy™ dyes are subject to proprietary rights of GE Healthcare and Carnegie
Mellon University, and are made and sold under license from GE Healthcare only for research and in vitro
diagnostic use. Any other use requires a commercial sublicense from GE Healthcare, 800 Centennial Avenue,
Piscataway, NJ 08855-1327, USA.
Laser safety information
Class 1 Laser Product.
Regulatory information
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
FCC information
WARNING: Changes or modifications to this unit not expressly approved by the party responsible for
compliance could void the user’s authority to operate the equipment.
NOTICE: This equipment has been tested and found to comply with the limits for a Class A digital device,
pursuant to Part 15 of the FCC Rules. These limits are designed to provide reasonable protection against
harmful interference when the equipment is operated in a commercial environment. This equipment generates,
uses, and can radiate radio frequency energy and, if not installed and used in accordance with the instruction
manual, may cause harmful interference to radio communications. Operation of this equipment in a residential
area is likely to cause harmful interference in which case the user will be required to correct the interference at
his or her own expense. Shielded cables must be used with this unit to ensure compliance with the Class A FCC
limits. This Class A digital apparatus meets all requirements of the Canadian Interference-Causing Equipment
Regulations. Cet appareil numérique de la classe A respecte toutes les exigences du Réglement sur le matériel
brouilleur du Canada.
History
Revision Date Change made
23-23437-00 2021-05 Initial release
23-23437(01) 2022-07 Changed page size to 8.5 × 11 inches.
Removed need for small particle filter assembly.
Changed procedures in Small Particle Detector chapter.
Modified cytometer troubleshooting.
Contents
1 ABOUT THIS GUIDE 7
What this guide covers 8
Conventions 8
About the BDFACSymphony™A1 documentation 8
Instrument technical support 9
2 INTRODUCTION 11
System overview 12
Cytometer overview 13
Control panel 15
Fluidics system 16
Sheath and waste tanks 21
Optics 22
Workstation 23
3 CYTOMETER SETUP 25
Starting the cytometer and computer 26
Preparing the sheath tank 27
Removing air bubbles 29
Preparing the waste tank 30
Preparing the fluidics 32
About the optical filters and mirrors 34
Custom configurations and baselines 35
4 MAINTENANCE 37
Maintenance overview 38
Cleaning the fluidics 39
Shutting down the cytometer 40
Flushing the system 40
Replacing the waste tank cap 42
Changing the sheath filter 43
Changing the Bal seal 46
Changing the sample tube O-ring 48
Cleaning or replacing the sheath gasket 49
Accessing the red laser detector array 49
5 OPTIMIZING CYTOMETER SETTINGS 51
Cytometer settings workflow 52
Verifying the configuration and user preferences 53
Running a performance check 55
Setting up an experiment 58
Creating application settings 62
Recording compensation controls 64
Calculating compensation 66
6 RECORDING AND ANALYZING DATA 67
Data recording and analysis workflow 68
Preparing the workspace 68
Recording data 69
Analyzing data 71
Reusing an analysis 75
7 TECHNICAL OVERVIEW 77
About fluidics 78
About optics 79
About electronics 84
8 TROUBLESHOOTING 89
Cytometer troubleshooting 90
Electronics troubleshooting 95
9 DETECTOR ARRAY CONFIGURATIONS 97
Fluorescence spectra 98
About configuration maps 99
About configurations 100
Base configuration polygon maps 101
10 SMALL PARTICLE DETECTOR 105
Overview 106
SPD components 106
Preparing the system for small particle detection 107
Confirming small particle detection 109
Aligning the small particle detector 113
Small particle detector troubleshooting 116
4BD FACSymphony™ A1 Flow Cytometer User's Guide
What this guide covers
This guide describes the procedures necessary to operate and maintain your BDFACSymphony™A1 flow
cytometer. Because many cytometer functions are controlled by BDFACSDiva™ software, this guide also
contains information about software features required for basic cytometer setup and operation.
This guide assumes you have a working knowledge of basic Microsoft®Windows®operation. If you are not
familiar with the Windows operating system, see the documentation provided with your computer.
Conventions
Introduction
The following table lists the safety symbols used in this guide to alert you to potential hazards.
Safety symbols
Symbol Meaning
Caution! Identifies a hazard or unsafe practice that could result in data loss, material damage, minor injury,
severe injury, or death
Biological hazard
Electrical hazard
Laser hazard
About the BDFACSymphony™A1 documentation
Introduction
This topic describes the documentation available with the BDFACSymphony™A1 flow cytometer.
Documentation USB Card
This user's guide is provided in PDF format on the Documentation USB card.
Help system
The help system installed with BDFACSDiva™ software includes the documents listed below. Access the
BDFACSymphony™A1 help system from the Help menu in the BDFACSDiva™ software. Internet access is not
required to use the help system.
The help system includes the following documents:
8BD FACSymphony™ A1 Flow Cytometer User's Guide
lBDFACSDiva™ Software Reference Manual: Includes instructions or descriptions for installation and setup,
workspace components, acquisition controls, analysis tools, and data management. Access this manual from
the BDFACSDiva™ Software Help menu (Help > Documentation > Reference Manual), or by double-clicking
the shortcut on the desktop.
lBDCytometer Setup and Tracking Application Guide: Describes how to use the BD®Cytometer Setup and
Tracking (CS&T) features in BDFACSDiva™ software.
Instrument technical support
Introduction
This topic describes how to get technical assistance.
Contacting technical support
If technical assistance is required, contact your local BDBiosciences customer support representative or
supplier.
When contacting BD Biosciences, have the following information available:
lProduct name, part number, and serial number
lVersion of BDFACSDiva™ software you are using
lAny error messages
lDetails of recent system performance
To contact customer support:
1. Go to bdbiosciences.com.
2. Select your region. You will see information in your local language.
3. Click Go.
4. Click the Support link for details for your local region.
Chapter 1 About this guide 9
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