Biochrom Ultrospec 50 Manuale utente

Biochrom Ultrospec 50, 60, 70 & 80

UV Visible Spectrophotometers

Accessories User Manual

TABLE OF CONTENTS:

1. HEALTH & SAFETY ...........................................................................................................................1

2. OPERATION .....................................................................................................................................1

3. INSTALLATION.................................................................................................................................1

4. PASSIVE ACCESSORIES.....................................................................................................................2

4.1. SINGLE CELL HOLDER ..............................................................................................................3

4.2. VARIABLE PATHLENGTH CELL HOLDER ...................................................................................4

4.3. WATER THERMOSTATED CELL HOLDER ..................................................................................4

4.4. FILM HOLDER ..........................................................................................................................4

4.5. TEST TUBE HOLDER.................................................................................................................5

4.6. PELTIER CONTROLLED FLUID CIRCULATOR.............................................................................6

5. ACTIVE ACCESSORIES ....................................................................................................................18

5.1. 8-POSITION CELL CHANGER ..................................................................................................19

5.2. 5-POSITION THERMOSTATTED CELL CHANGER ....................................................................19

5.3. SIPPER & FLOWCELL..............................................................................................................20

6. INSTALLING THE INTERNAL PRINTER ............................................................................................24

Issue 02 1

1. HEALTH & SAFETY

2. OPERATION

The operation of all Biochrom Ultrospec accessories is described in the relevant user manual.

3. INSTALLATION

Before installing any accessory in the Biochrom Ultrospec you must ensure that the instrument is

disconnected from the mains.

Note: The images in this manual show the installation of accessories in a double beam instrument.

Accessories are installed in split beam instruments in exactly the same way. All accessories detailed

in this manual are available for both split beam (Ultrospec 50) and double beam instruments

(Ultrospec 60, 70 & 80).

Care must be taken when handling all heated accessories

There are no bio-hazardous materials within the unit; however this unit could be used with

bio-hazardous samples. Before using the instrument the customer should have in place

decontamination procedures designed to protect laboratory workers from occupationally acquired

infections. The sample chamber cell holders are removable and may be decontaminated using the

appropriate disinfectant for the bio hazard in question, rinsed with distilled water and then allowed

to dry. The sample chamber and exterior may be wiped with a suitable disinfectant cleaning wipe.

Decontamination. Equipment returned for repair should include an appropriate

decontamination certificate

It is the responsibility of the customer to ensure that the user of the equipment is provided

with a safe working environment.

Any chemicals used in Analyses should be used, stored and disposed of in accordance with

manufacturer’s guidelines and local safety regulations

Toxic Fumes. Efficient laboratory ventilation must be provided when working with volatile

solvents or toxic substances

Waste disposal. Disposal of some solvents and chemicals may be classed as hazardous waste

and must be dealt with in accordance with local regulatory practice.

Personal protective equipment. This is not required to operate the unit but the samples measured

may require PPE. A local risk assessment should be carried out.

Toxic fumes.

Issue 02 2

4. PASSIVE ACCESSORIES

Accessories that do not interact with the instrument’s software are named passive accessories.

There installation is described below:

To allow easy access to the thumbscrew at the front of the accessory open the cell chamber lid and

lift out the front panel (below left).

With the front panel removed the accessory is removed and replaced as outlined below:

1. Loosen the front thumb screw by turning anticlockwise.

Issue 02 3

2. With the thumb screw fully unscrewed, lift the accessory

and gently pull forward.

3. To insert the desired accessory, lift the front end, slide into

the brackets at the back of the cell chamber (indicated by the

two arrows) and tighten the thumb screw by turning

clockwise.

With the accessory inserted simply replace front panel, switch on the instrument and it will be ready

to use.

4.1. SINGLE CELL HOLDER

The single cell holder is designed to take

measurements of 10 mm pathlength cuvettes. All

Biochrom Ultrospec are supplied with a single cell

holder as standard.

Issue 02 4

4.2. VARIABLE PATHLENGTH CELL HOLDER

The variable pathlength cell holder is designed to use 1,

2, 5, 10, 20, 50 & 100 mm pathlength cells. To

accommodate different pathlengths the clips should be

moved to secure the cell and spacers used as required.

4.3. WATER THERMOSTATED CELL HOLDER

The water thermostated cell holder is designed to

work with water circulators to achieve constant

temperatures in the range 5 to 80 °C. The accessory

has to be connected to an external fluid circulator so

that the water flows into the bottom of the cell

holder (the right hand inlet) and flows out through

the top of the cell holder (the left hand outlet).

4.4. FILM HOLDER

The film holder is designed to measure the

absorbance of solid materials including thin flexible

sheet materials such as plastic, acrylic, gelatine.

Applications also include the quality control of

tinted optical lenses.

Issue 02 5

4.5. TEST TUBE HOLDER

The test tube holder allows samples to be measured

in a test tube of between 10 and 24mm diameter

and up to 160mm tall

Issue 02 6

4.6. PELTIER CONTROLLED FLUID CIRCULATOR

Content

4.6.1. GENERAL INFORMATION ................................................................................................7

4.6.1.1. General Warnings ...................................................................................................7

4.6.1.2. Quality, Reliability and Safety .................................................................................7

4.6.1.3. SAFETY MARKINGS..................................................................................................8

4.6.2. INSTRUMENT DESCRIPTION............................................................................................8

4.6.2.1. Controller front and rear panel...............................................................................9

4.6.2.2. Specifications ..........................................................................................................9

4.6.2.3. User interface........................................................................................................11

4.6.3. INSTALLATION...............................................................................................................12

4.6.3.1. Checking the serial number ..................................................................................12

4.6.3.2. Checking mains supply and fuses..........................................................................12

4.6.3.3. Instrument set-up .................................................................................................13

4.6.4. OPERATING INSTRUCTIONS ..........................................................................................14

4.6.4.1. Manual operation .................................................................................................14

4.6.5. MAINTENANCE..............................................................................................................16

4.6.5.1. Cleaning the instrument .......................................................................................16

4.6.5.2. Water level alarm..................................................................................................16

4.6.6. TROUBLESHOOTING......................................................................................................17

4.6.6.1. Audible alarm........................................................................................................17

Issue 02 7

4.6.1. GENERAL INFORMATION

4.6.1.1. General Warnings

Check the voltage selector

Always disconnect the mains plug before starting any work inside the instrument

Set the temperature to the

required values on power on

If using substances dangerous for health and

the environment, observe the laws and

the standards in force in the laboratory where this instrument is used

If using flammable or explosive substances take the necessary precautions

4.6.1.2. Quality, Reliability and Safety

This equipment has been designed with emphasis on QUALITY, RELIABILITY and SAFETY, and we will

accept responsibility for these aspects only if the following conditions are met:

Electrical installation of the room or building in which the equipment is to be used must

comply with regulations specified in the country where the equipment is to be used.

The equipment must be used in accordance with the instructions for use provided by

BIOCHROM.

All modifications and repairs to the equipment must be carried out by BIOCHROM or its

authorised Agents.

Modifications must not be carried out unless they conform to approved Engineering Service

Information issued according to the appropriate BIOCHROM procedure.

The equipment installation must be carried out in accordance with local requirements for

responsibility and warranty.

Issue 02 8

4.6.1.3. Safety Markings

CE mark

The "CE" mark for this product makes reference to the directives

•89/336/EEC amended by 93/68/EEC for electromagnetic compatibility

•73123/EEC amended by 93/68/EEC for low voltage

for any purpose other than that for which it was originally supplied. The publication may not be

reproduced in part or in whole without written consent of BIOCHROM. In order to maintain and

improve standards of manufacturing, methods of functioning and to increase reliability, BIOCHROM

equipment is periodically reviewed. For this reason, the contents of this publication are subject to

change without notice.

4.6.2. INSTRUMENT DESCRIPTION

The Controller is a Peltier effect thermostatting system using an internal sealed liquid circuit allowing

full compatibility and connection to all thermostattable accessories.

The Peltier effect is based on the reversed thermoelectric effect. This is a phenomenon in which a

temperature difference is caused by a flow of current through a closed circuit consisting of two

different metals.

The Controller provides temperatures ranging from 20 °C to 60 °C with ±1 °C stability and ±0.1 °C

repeatability. The features of the Controller include:

Peltier effect for heating and cooling the liquid of circulation

Internal flow pump to circulate the liquid

The Controller can operate in either manual or remote control via a USB port

The Controller is supplied with one set of thermally insulated connection tubes to connect

the Controller to any thermostattable accessory

The Controller is supplied with a water level sensor to ensure correct operation

The Controller is supplied with high temperature sensor to avoid damaging the system and

malfunctions

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