Biogal PCRun Manuale utente

Table of Contents

Contents of Box

Starting the PCRun Reader

Assembly of PCRun Reader

Choosing the Default Test Protocol

Running a PCRun Reaction

General Feature

Safety Precautions

User Responsibility

Analysis of Results

Reset PCRun Reader

Aborting a Run

Downloading Files from the PCRun Reader

Deleting Files from the PCRun Reader

Analyzing PCRun Graphs

Instructions for Use of the PCRun Reader Simulator

Unpacking and Assembling

Operation Guide

Page

Unpacking and Assembling

Fig. 4: USB /Micro-USB

connector

Fig. 2: Power cable

Fig. 7: TransformerFig. 5: Stylus

Fig. 1: Reader

Fig. 3: On/off switch

Fig. 6: USB Portable

Memory Stick

(present only in certain

models)

Contents of Box

Attention!

Before installing the PCRun Reader please pay attention to the following:

Do not place the Reader in an area which receives direct light (natural or articial). During

the amplication process light is emitted from the reagents. The generated light is collected,

interpreted and transformed to a readable signal of positivity or negativity. Strong external

light may cause background noise which may be translated into a false positive signal.

Do not place the Reader on the same table with apparatus that my cause vibrations

(centrifuge, vortex, shakers etc). Vibrations will result in unstable readings.

Remove the PCRun Reader (Fig. 8) from the protective packaging and rest on a solid, level

surface allowing approximately 25 cm of clearance space above the unit for opening. Allow

5 cm distance from any wall or object to allow for proper ventilation. Make sure that the

Reader is not housed in area with strong light.

Assembly of PCRun Reader

Fig. 8: Front view

Lid

LCD touchscreen

Indicator light

Stylus

Fig. 9: Back view

On-off switch

Portable memory

port

Mini USB port Transformer

Outlet

Connect the transformer (Fig. 7) to the power cable (Fig. 2) and then to the back of the PCRun

Reader (Fig. 9). If the Reader comes with an external on/ off switch (Fig. 3) connect it to both the

back of the PCRun Reader and to the transformer cable. The transformer will adapt the voltage

to 220 or 110.

The PCRun Reader is a complete system which contains a heater and luminometer to be used in

conjunction with PCRun reagents designed for the amplication process and analysis of PCRun

molecular reactions. The unit contains a graphical LCD touch panel on which the nal results are

displayed.

• Users are responsible for familiarizing themselves with the product instructions and

limitations.

• External factors such as sample quality and preparation, as well as testing protocols and

laboratory techniques, may inuence the nal results. It is the user’s responsibility to select

the proper sample material which meets the criteria of the chosen test.

• As with any test method utilized for in vitro diagnostics, the results obtained from use of the

PCRun technology should be interpreted together with the results of other tests.

• Operate the instrument on a solid dry surface away from a strong light source.

• Ensure the laboratory electrical supply is appropriate and surge protected.

• The PCRun Reader should be switched off when not in use.

• Do not open the casing of the Reader.

• Maintenance of the unit must be carried out by Biogal personnel only.

General Feature

User Responsibility

Safety Precautions

Fig. 10 | Model B

Power cord

connection to

transformer

Connection to

Reader

Fig. 10 | Model A

Prior to using the PCRun Reader make sure, that it has been set up according to the

“Unpacking and Assembling” instructions.

1.1

Turn on the PCRun Reader using the on/off switch located on the power cord

(Fig. 10, Model A) or on the back of the Reader (Fig.10, Model B).

Operation Guide

1. Starting the PCRun Reader

1.2

The following display will appear briey (Fig 11). The

PCRun Code # and the rmware that is installed in

the Reader are located on the bottom of the screen.

1.3

The main screen (Fig. 12) will replace the Logo

display. The following details will be displayed:

2.1

The default test protocol setting is GENERIC and

is suitable for most of the PCRun reactions.

Alternate protocols may be recommended for

certain kits. Prior to using the Reader, check the kit

instructions to determine which protocol is suitable

for your test. In the case that a different protocol is

recommended, the following instructions will explain

how to change the program temporarily or to dene

a new default program. Using the stylus, activate

the Test: GENERIC tab (Fig. 12). The names of the

various protocols will appear in the window. Choose

the recommended protocol (Fig. 13).

• Warm up ?

• Test: GENERIC

• Settings

• Files

• Present date and hour

• Thermometer with ambient temperature

2. Choosing the Default Test Protocol

Fig. 11

Fig. 12

Fig. 13

3.1

Using the stylus press Warm Up? (Fig. 12). The

message on the screen will change to grey and

the words Warming up will appear. In addition, the

indicator light and the thermometer will change to

orange (Fig. 14).

If you will be using the protocol for a single application press Select located on the bottom right

hand side of the screen. If you choose to make the new protocol the default setting, press Make

default located on the bottom left side of the screen. Follow by pressing Select.

3. Running a PCRun Reaction

3.2

Once the temperature of the Reader has reached

its target (60°C) the message will change to Run test.

The indicator light and the thermometer on the touch

screen will change to green. The temperature noted

above the thermometer will be 60°C (Fig. 15).

Fig. 15

®Fig. 14

3.3

Open the lid of the Reader and load the reaction tubes

into the internal wells. Take note of where the tubes

are placed (Fig. 16).

Fig. 16

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