Drawell DW-K2800 Manuale utente
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DW-K2800Nucleic Acid Analyzer
Please read operating manual before installation and operation.
Drawell International Technology Limited
Shanghai Drawell Scientific Instrument Co.,Ltd
Add : Suite 1506,Lane581 XiuChuan Rd., Greeland
Max-Mall,PuDong,Shanghai,China
Tel: 0086 21 50966080
Fax: 0086 21 50966080
Web : www.drawell.com.cn
Email : [email protected]
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1. User Notice
This manual will guide you through the proper use of the DW-K2800 Nucleic Acid Analyzer.
Please read this manual carefully before you install, configure and use it.
This manual is copyrighted by Shanghai Drawell Scientific Instrument Co.,Ltd, without the
authorization of Shanghai Drawell Scientific Instrument Co.,Ltd This manual is not free to modify
and copy or publish to the Internet.
Shanghai Drawell Scientific Instrument Co.,Ltd. assumes no responsibility for any loss or
loss of data caused by data loss and other accidents caused by user operations mentioned in this
manual. Shanghai Drawell Scientific Instrument Co.,Ltd is not responsible for accidents caused by
potentially bad operations not mentioned in this manual.
If you follow the above rules, from now on, you can enjoy the convenience of the
DW-K2800 nucleic acid analyzer for your work and experiment.
Packing List:
The following items are included in the package of each product. If you find that the item
listed below is different after opening the package for the first time, please contact Shanghai
Drawell Scientific Instrument Co.,Ltd or the product distributor to provide or replace it for free.
Related items.
Item Name
Number of pieces
DW-K2800 Nucleic Acid Analyzer
1
12V power adapter
1
12V power adapter to socket connection
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Daily maintenance
1.light source. The life of the light source is limited. In order to prolong the service
life of the light source, do not turn on the light source when the instrument is not in
use. The continuous use time of the instrument should not exceed 3 hours. If you need
to use it for a long time, it is best to pause for 30 minutes.
2.To avoid dust and contamination of the instrument, cover the dust cover when you
stop working.
3.The sample base should be cleaned regularly with a cotton swab and 75% alcohol.
After each experiment, scrub with water once to keep the test head clean.
4.Wipe the upper and lower bases with clean, lint-free paper before the instrument can
perform the next sample test.
5.If the instrument does not need to be energized regularly, it should be no less than
20~30min each time to keep the whole machine dry and maintain the performance of
electronic components.
Working environment requirements
1.The instrument should be placed in a dry room with a relative humidity of no more
than 85%.
2.The instrument should be placed on a sturdy, stable work surface and protected
from strong vibrations or constant vibration.
3.Indoor lighting should not be too strong, and direct sunlight should be avoided.
4.The fan should not be directly blown to the instrument to prevent the light source
lamp from affecting the normal use of the instrument due to unstable illumination.
5.Keep away from high-intensity magnetic fields, electric fields, and electrical
equipment that generate high-frequency waves.
6.The power supply voltage to the instrument is AC 100-220V, the frequency is
50/60Hz, and a good ground wire must be installed. It is recommended to use an
electronic AC voltage regulator or AC constant voltage regulator with a power of
more than 1000W to enhance the anti-jamming performance of the instrument.
7.Avoid use in places where corrosive gases such as hydrogen sulfide are present.
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2. Product Introduction
The DW-K2800 is a measuring (230, 260, 280 nm) nucleic acid analyzer. The
instrument can accurately measure 1~2μL of micro-samples with good repeatability.
Comes with a computer, display and 2 USB ports, built-in software, no need to install,
can connect USB devices such as mouse, keyboard, printer, U disk, wireless network
card. The low concentration detection is more stable, the high concentration detection
range is wider, 200 times that of the conventional UV-Vis spectrophotometer, no
dilution is required, and no baseline calibration is required. The micro-mode sample is
used in a small amount, and the minimum measurement volume is 1 μL, which can be
used to detect nucleic acids, proteins, and the like. All data is automatically saved,
which is convenient for output data statistics, and the software is easy to grasp.
Principle
After the spectrophotometer is in the blank, the instrument automatically records
the spectral result of the blank reference solution and saves it as the light intensity
reference value of the wavelength. When the sample is tested, the light intensity
transmitted through the sample will be recorded. The transmitted light intensity of the
sample and the transmitted light intensity of the blank control are calculated according
to the following formula:
In this way, the absorbance at a specific wavelength can be calculated from the
transmitted light intensity of the sample and the blank.
The relationship between sample concentration and absorbance is determined by
Lambert-Beer's law:
A=ε b c
Among: A＝Absorbance value（A）
ε＝Wavelength dependent molar extinction coefficient（unit L/mol*cm）
b＝Optical path（unit cm）
c＝Sample concentration（unit mol/L）
Reference solutions, or blank solutions, are usually solvents that melt the
targeting molecule, which has the same pH and ionic strength as the sample solution.
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Application Range
The DW-K2800 Nucleic Acid Analyzer can be used to measure:
Nucleic Acid：
1.The concentration and purity of nucleic acid samples, including double-stranded
DNA, single-stranded DNA, RNA, and other nucleic acids.
2.Microarray samples can simultaneously detect the concentration of nucleic acid and
fluorescent dye, and directly give the concentration value
Protein：
A280 measured protein sample concentration, including BSA, IgG, Lysozyme,
Labels;
Instrument parameters
1. Optical path: 1, 0.1, 0.05mm adjustable
2. Minimum sample volume: 1μl
3. Light source: long life xenon lamp
4. Detector type: 2048 linear CCD array
5. Wavelength range: 230, 260, 280 nm
6. Wavelength accuracy: ±1
7. Wavelength resolution: ≤ 2nm (FWHM@Hg 546nm) Light absorption rate
accuracy: 0.002Abs
8. Light absorption accuracy: 1% (0.76 absorbance at 350nm)
9. Absorbance value range: 0.02-100 (equivalent to 10mm optical path)
10. Concentration detection range: 2~15000ng/μl (DS-DNA) 0.1~100mg/ml
(BSA)
11. Detection period: less than 5 seconds
12. Working voltage: DC 12V/4A
13. Maximum power: 25W
14. Sample base: stainless steel
15. Dimensions: 30cm*20cm*18cm
16. Weight: 3.5kg
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Instrument structure
Fig1 Side view
1.Screen 2. Detection arm 3.platform
The instrument has a built-in computer and does not require an external
computer. When the sample is detected, the sample can be dropped on the susceptor
to form a liquid column, and the ultra-micro mode is used to make the detection result
more precise.
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3
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Fig 2 Back view
1.Power jack 2. Switch 3.USB port
After the power is turned on, turn on the main switch, press and hold the system
switch for 3 seconds, then release, the instrument system starts, and it will directly
enter the test program interface after booting. You can use the USB interface to
connect an external mouse, keyboard or USB flash drive.
If the instrument is not turned on, you can press on the desktop Button,
enter the software.
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3. Detection mode
1. Blank Use a pipette to take 1-2.5μl (depending on the actual situation)
buffer and drop it on the test pedestal (the base material is stainless steel and the
center point is quartz), the lower test head is the receiving end, and the upper test head
is The transmitting end.
2. Sample measurement Use a pipette to take 1-2.5 μl of the sample to be
tested and add it to the test pedestal. The protein sample should be tested with a
sample size of 2.0 μl.
3. Due to the inconsistent tension of different samples, in order to make the
sample form a liquid column better, the recommended sample detection amount is as
follows (the user can adjust according to the actual situation):
Nucleic acid solution: 1.5μl
Protein solution: 2.0μl
Other samples: 2μl
1.1 Basic operation
1. Lift the sample arm and pipette 1~2μL of sample solvent onto the susceptor.
2. Lower the sample arm and start the absorbance test using the software on your
computer. A sample column is automatically pulled between the upper and lower
fibers and then tested.
3. When the test is complete, lift the sample arm and clean the sample on the
upper and lower bases with clean, lint-free paper. This wipes the sample to avoid
residue on the susceptor.
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Software interface introduction
After the system is turned on, the nucleic acid detection interface is entered by
default. As shown.
1.Function control area：According to the substance you want to test, select the
corresponding test sample type, which is divided into Nucleic Acid NA and
Protein.
2.Open and Open Record：the date file in the list on the left to view the history
record.
3.Data display area: You can view the absorbance and ratio at 230, 260, and 280
nm.
4.Seting，You can view the type of nucleic acid or protein sample to be detected.
The nucleic acid has three kinds of dsDNA, ssDNA and RNA. The protein has
A280, BSA, IgG, Lysozyme, etc., and then click "Edit" to re-select.
5.Blank: Click to measure the blank buffer to measure the sample concentration.
6.Measure: After placing the sample, click to measure and read the sample
absorbance value, ratio, and concentration value.
7.Check: Click to view spectral connections, light intensity.
8.Shutdown: Click on the instrument will shut down, after a few tens of seconds
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can be powered off
9.Save As: List the data of the next list in the form of an Excel spreadsheet.
10.Move Up：Move the data strip up.
11.Name: Name the sample to be measured.
12.Key: Click to display the soft keyboard.
13.Delete: Click to delete all records or one in the list on the left.
14.
Indice
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