Table of Contents
8001009 Cellometer® Auto T4 User Manual Rev C iv
Cell Type Drop-down Defaults....................................................................................................................... 26
Importing Cell Types ..................................................................................................................................... 26
From Nexcelom Cell Library..................................................................................................................................................26
From Custom Libraries.......................................................................................................................................................... 27
Deleting Cell Types........................................................................................................................................ 27
From Home Screen................................................................................................................................................................27
From Import/Export Cell Type Screen ..................................................................................................................................28
Clearing Cell Types from Drop-down............................................................................................................. 28
Exporting Cell Types to a Library ................................................................................................................... 29
CHAPTER 7. SAMPLE PREPARATION ...................................................................................... 31
Preparing Cellometer Chambered Slides .............................................................................................. 31
Loading Sample into Counting Chamber .............................................................................................. 32
CHAPTER 8. COUNTING AND ANALYSIS WORKFLOW............................................................. 33
Perform Cell Counting .......................................................................................................................... 33
Review Count Results ........................................................................................................................... 35
Best Practices and Workflow Tips ................................................................................................................. 36
Saving Data and Images ................................................................................................................................ 36
Printing Data................................................................................................................................................. 36
View Cell Size Analysis.......................................................................................................................... 37
Calculate Sample Adjustment............................................................................................................... 38
Reference of Cell Sample Images ......................................................................................................... 39
CHAPTER 9. CELL TYPES......................................................................................................... 41
Editing Cell Types.................................................................................................................................. 41
Cell Type Screen............................................................................................................................................ 41
Adjusting Cell Diameter Parameters ............................................................................................................. 43
Adjusting Trypan Blue Viability Parameters................................................................................................... 44
Adjusting Decluster Parameters for Clumpy Cells.......................................................................................... 45
Sample Clumpy MCF7 cells...................................................................................................................................................46
Disabling Decluster for Small Cells.......................................................................................................................................46
Creating New Cell Types ....................................................................................................................... 48
Using Cell Type Wizard.................................................................................................................................. 48
Creating Cell Type Manually.......................................................................................................................... 48
Nexcelom Cell Library........................................................................................................................... 49
CHAPTER 10. DATA MANAGEMENT....................................................................................... 51
Saving Data and Images........................................................................................................................ 51
Manually Saving Data ................................................................................................................................... 51
Manually Saving Images................................................................................................................................ 51
