PP Systems CIRAS-3 Manuale utente

CIRAS-3

Portable Photosynthesis System

Operation Manual

Version 1.09

7th August 2017

PP Systems

110 Haverhill Road, Suite 301

Amesbury, MA 01913 U.S.A.

Tel: +1 978-834-0505 Fax: +1 978-834-0545

Email: support@ppsystems.com URL: www.ppsystems.com

CIRAS-3 Operation Manual V. 1.09 2 support@ppsystems.com

Contents

Welcome .....................................................................................................................................................10

User Registration ........................................................................................................................................11

Service & Warranty.....................................................................................................................................12

Returning equipment to PP Systems ......................................................................................................12

Contact Information.....................................................................................................................................13

Unpacking and Storage of Your Equipment............................................................................................14

Unpacking............................................................................................................................................14

Storage ................................................................................................................................................14

CIRAS-3 Tutorial & Basic Operation...........................................................................................................15

Let’s Get Started......................................................................................................................................16

Charge internal battery packs..............................................................................................................16

Inspect desiccants ...............................................................................................................................16

Insert a fresh CO2cartridge.................................................................................................................17

Connect the PLC3 Universal Leaf Cuvette to the CIRAS-3 console...................................................18

Connect light unit to PLC3 Universal Leaf Cuvette .............................................................................18

Power up the CIRAS-3 System ...........................................................................................................19

System Warm up .................................................................................................................................19

Setting up the CIRAS-3 System ..........................................................................................................20

Setting up the graphical display for data plots.....................................................................................21

Let’s check out both the numerical and graphical display...................................................................21

Let’s have a look at the environmental controls ..................................................................................23

It’s about time we start taking some data on a real plant ....................................................................25

Placing the leaf inside the cuvette .......................................................................................................27

What should I be looking for at this point?...........................................................................................27

Quick Start...................................................................................................................................................31

Section 1. Technical Specification .............................................................................................................33

Section 2. What’s new with CIRAS-3?.......................................................................................................36

General Changes to CIRAS-3 Hardware.............................................................................................36

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Changes to CIRAS-3 Software............................................................................................................38

Section 3. Summary of System Design .....................................................................................................40

Overview and Theory ..............................................................................................................................40

Section 4. System Components and Assembly.........................................................................................44

CIRAS-3 Main Console........................................................................................................................44

System Power .........................................................................................................................................44

Power Supply Adapter.........................................................................................................................44

Battery Pack.........................................................................................................................................45

Battery Pack Installation ......................................................................................................................46

Charging Batteries inside the CIRAS-3 Console.................................................................................47

CIRAS-3 Rear Console ...........................................................................................................................48

USB Flash Drive Ports.........................................................................................................................48

Battery Charging Socket (EXT PWR) and LED...................................................................................49

Power...................................................................................................................................................49

Mini-USB (PC) .....................................................................................................................................49

PLC Connections.................................................................................................................................49

CO2Cartridge Holder...........................................................................................................................49

CO2Cartridges.....................................................................................................................................50

How do I determine the state of the CO2cartridge?............................................................................50

Gas Ports and Link Pipe......................................................................................................................51

Auxiliary Connector..............................................................................................................................51

Zero Column ........................................................................................................................................51

CO2/H2O Control Columns and H2O Equilibrator.................................................................................52

PLC3 Series Leaf Cuvettes.....................................................................................................................53

PLC3 Universal Leaf Cuvette ..............................................................................................................53

PLC3 Narrow Leaf Cuvette..................................................................................................................53

PLC3 Conifer Leaf Cuvette..................................................................................................................53

PLC3 Temperature Control .....................................................................................................................54

Temperature Measurement .................................................................................................................54

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Temperature Control Type...................................................................................................................54

LCD on Leaf Cuvette ...........................................................................................................................55

PAR Measurement ..................................................................................................................................56

Light Control (Optional) ...........................................................................................................................57

PLC3 Universal LED Light Unit (RGBW).............................................................................................57

PLC3 Narrow and Conifer LED Light Unit ...........................................................................................58

CFM-3 Chlorophyll Fluorescence Module ...........................................................................................59

Section 5. CIRAS-3 Console Software ......................................................................................................60

Overview..................................................................................................................................................60

Basics of Navigating Console Screens and Menus.............................................................................61

Numeric View of Data and Control Variables ......................................................................................62

Section 6. Startup Routine and System Stability Checks ..........................................................................66

Before You Power Up CIRAS-3 ..............................................................................................................66

Startup and Warm up...........................................................................................................................67

CO2Cartridge Status...............................................................................................................................68

Changing a CO2Cartridge.......................................................................................................................70

Checking Stability - Before You Place a Leaf in the Chamber............................................................70

Survey Measurements – Some Tips....................................................................................................71

Additional Filtering in Dirty/Dusty Environments..................................................................................72

Section 7. Basic Functions - Viewing and Controlling CIRAS-3................................................................73

Settings (F2)............................................................................................................................................73

Settings Graph Set (F5).......................................................................................................................78

Controls (F3)............................................................................................................................................79

Controls Graph Set (F5).......................................................................................................................80

Toggle View (F4)..................................................................................................................................80

Reviewing and Transferring Data Files...................................................................................................82

Transfer Data (F6) ...............................................................................................................................82

Section 8. Operations.................................................................................................................................84

Operations (F1) .......................................................................................................................................84

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Recording Options...................................................................................................................................84

Manual Recording................................................................................................................................84

Timed Recording..................................................................................................................................86

Response Curves ................................................................................................................................87

Z-Diff Bal (F7) ......................................................................................................................................94

Set Clock .................................................................................................................................................95

View Saved (F4)......................................................................................................................................96

Calibration................................................................................................................................................98

Recalibrate...........................................................................................................................................98

Calibration Setup .................................................................................................................................99

CO2Calibration....................................................................................................................................99

H2O Calibration..................................................................................................................................100

Find Max C ............................................................................................................................................101

Store Diff Bal .........................................................................................................................................102

LED Calibrate.....................................................................................................................................105

PAR Calibrate....................................................................................................................................106

Diagnostics ........................................................................................................................................107

IRGA (F2)...........................................................................................................................................107

PLC (F3) ............................................................................................................................................108

Flow (F4)............................................................................................................................................110

Power (F5) .........................................................................................................................................111

APA (F6) ............................................................................................................................................112

Report (F7).........................................................................................................................................113

Firmware Upgrade.................................................................................................................................114

Section 9. CIRAS-3 Console and PC Utility Software .............................................................................116

CIRAS-3 Console Software...................................................................................................................116

Installation Instructions for Main CIRAS-3 Console...........................................................................116

Uninstall Instructions..........................................................................................................................116

PC Utility Software.................................................................................................................................117

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Installation Instructions for PC Utility.................................................................................................117

Uninstall Instructions..........................................................................................................................117

PC Utility Operation ...........................................................................................................................118

Create/Edit Response Scripts............................................................................................................119

Create/Edit Settings Files ..................................................................................................................121

Remote Display..................................................................................................................................123

Section 10. Measuring Chlorophyll Fluorescence with CFM-3................................................................127

Overview of Chlorophyll Fluorescence..................................................................................................127

The Basics .........................................................................................................................................127

Calculations Used for Chlorophyll Fluorescence Parameters...........................................................129

Chlorophyll Fluorescence Measurements – Setup and Operation........................................................130

CFM-3 Set Up........................................................................................................................................131

CFM-3 Settings..................................................................................................................................132

CFM-3 Controls..................................................................................................................................135

Measurement of Fm’ with CIRAS-3.......................................................................................................137

Section 11. Closed System Measurements.............................................................................................140

Soil CO2Efflux and Net Canopy CO2Flux............................................................................................140

Theory....................................................................................................................................................140

FCO2Units for measurement of Soil CO2Efflux................................................................................141

References.........................................................................................................................................142

Electrical and Pneumatic Connection between the CIRAS-3 and SRC-1 / CPY-4 ...........................142

Electrical and Pneumatic Connection between the CIRAS-3 and SRC-2 / CPY-5 ...........................144

Configuring the CIRAS-3 console for measurement of Soil Respiration or Canopy Assimilation.....144

Settings (F2) ......................................................................................................................................145

Do I Use Linear versus Quadratic......................................................................................................147

Closed System Operation......................................................................................................................147

Measurement Sequence .......................................................................................................................150

Initial Test Measurement Sequence..................................................................................................151

Recording Measurements .....................................................................................................................151

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Section 12. Data Output...........................................................................................................................153

Photosynthesis Data..............................................................................................................................153

Photosynthesis and Chlorophyll Fluorescence Data.............................................................................155

Closed System Operation Data.............................................................................................................158

Summary of the Gas Exchange Equations Used in CIRAS-3...............................................................159

Mass Flow Equation...........................................................................................................................159

Transpiration......................................................................................................................................159

Leaf Temperature ..............................................................................................................................160

Saturation Vapor Pressure ................................................................................................................161

Stomatal Conductance.......................................................................................................................161

Net Photosynthesis............................................................................................................................161

Intercellular CO2Concentration.........................................................................................................162

Physical Constants Used in Equations..............................................................................................163

Definition of Symbols.........................................................................................................................163

Section 13. Troubleshooting & Diagnosis................................................................................................165

System Power .......................................................................................................................................165

CO2Measurement and Control.............................................................................................................166

H2O Measurement and Control.............................................................................................................168

Temperature Measurement and Control...............................................................................................169

PAR Measurement and Control ............................................................................................................170

Flow Control.......................................................................................................................................171

Data Management.................................................................................................................................172

Status Codes Displayed on the CIRAS-3 LCD..................................................................................173

Other Status Codes/Messages..........................................................................................................177

Section 14. Routine Maintenance............................................................................................................178

CIRAS-3 Main Console .........................................................................................................................178

Battery Pack (Li-ion Battery)..............................................................................................................178

Battery Removal ................................................................................................................................179

Battery Storage..................................................................................................................................179

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Battery Disposal.................................................................................................................................179

External Battery Pack (For extended operation in the field)..............................................................180

Air Sampling Pumps (Reference and Analysis).................................................................................180

Replacement of Reference and Analysis Pump................................................................................181

Servicing the Air Sampling Pump (Reference & Analysis)................................................................181

Air Supply Pump................................................................................................................................182

Air Filters............................................................................................................................................183

External Air Inlet Filter Assembly (Part Number STD558).................................................................184

Desiccants and Absorber Columns ...................................................................................................184

Zero Column Assembly......................................................................................................................185

CO2& H2O Control Column...............................................................................................................186

Soda Lime..........................................................................................................................................187

Drierite ...............................................................................................................................................187

Molecular Sieve .................................................................................................................................188

Molecular Sieve Repackaging ...........................................................................................................189

Foam Filters.......................................................................................................................................189

Absorber Filters..................................................................................................................................190

“O” Rings............................................................................................................................................190

CO2Regulator....................................................................................................................................190

CO2Calibration......................................................................................................................................192

H2O Calibration......................................................................................................................................193

PLC3 Leaf Cuvettes ..............................................................................................................................193

Cuvette Gaskets ................................................................................................................................193

Checking For Leaks Associated With the PLC3................................................................................194

PLC3 Leaf Cuvette Head Adjustment................................................................................................195

PLC3 Pneumatic Connector ..............................................................................................................196

Environmental Sensors .........................................................................................................................196

Temperature ......................................................................................................................................196

PAR....................................................................................................................................................196

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Boundary Layer Determination (rb)........................................................................................................197

Changing PLC3 Universal Head Plates.............................................................................................200

Replacement of PLC3 Glass Window (For Use with CFM-3)............................................................201

Section 15. Consumables/Spares............................................................................................................202

Desiccants and Absorber Columns.......................................................................................................202

Pumps & Air Filters................................................................................................................................202

CO2/H2O Control....................................................................................................................................203

Batteries & Power Supplies...................................................................................................................203

Cables/Data Storage.............................................................................................................................203

Leaf Cuvette Accessories......................................................................................................................203

CIRAS-3 Operation Manual V. 1.09 10 support@ppsystems.com

Welcome

Thank you very much for purchasing our CIRAS-3 Portable Photosynthesis System. We greatly

appreciate your business and we look forward to working with you and your research team for many

years to come.

This operation manual is based on our software Version 1.09 and above along with the following system

firmware and above:

•FW Version: V. 1.04 and above

•Air Version: V. 3.02 and above

•IRGA Version: V. 3.06 and above

•PLC Version: V 3.14 and above

To check which software and firmware versions are currently installed on your CIRAS-3 go to Help (F8) >

About (F2):

For users running V. 1.09 console software along with latest firmware, future software and firmware

updates can be made locally without the need to return the system to our factory. Our latest software and

documentation can be downloaded free of charge from our website if you are registered with us (See

User Registration on page 11). For users running older software and firmware versions, the CIRAS-3,

This product is manufactured in accordance with CE

requirements. For more information on system

conformity please get in contact with PP System.

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