STEINDORFF CX50 Series Manuale utente

INSTRUCTION MANUAL

360° Rotatable Head

Rubber Feet

Nosepiece

Achromatic color-coded

DIN Objectives

Double layer

Machanical Stage

Coaxial Coarse

and

Fine Focusing

Iris Diaphragm

LED Illuminator

Light Intensity Control

STEINDORFF®

CONTENTS

A.Illustration of Microscope…………………………….……..………COVER

B.Unpacking and Preparation for Use…..………….…...…….............

……

C.Features / Denitions…………………………..……..…….............…….. 2

D.Operating Procedure…….…………………………….........................… 4

E.Maintenance and Care …….………………………………....…..…........ 6

F.Electrical Maintenance ………..………….……

...............…..............

..... 7

G.Troubleshooting….………

…….…………….............

.................…...…...9

H.OptionalDigitalCameras…………………..……...........………...….…...10

- 1 -

B. UNPACKING & PREPARATION

our The microscope is supplied with an expanded two-part

Styrofoam/polystyrene case. This case should be used for

storage, transport, and shipping. Microscopes that are not

properly packed are usually damaged during transport and

shipping. KEEP STYROFOAM BOX FOR FUTURE USE

FOR SHIPPING AND/OR REPAIR.

Unpack the microscope and its parts carefully. Do not throw

away any boxes or packing materials until the contents of

the shipping container have been checked against the pack-

ing list of your order. In an area that is clean, dry and dust

free, place the microscope and parts on a sturdy desk or

table for initial use and/or assembly.

DO NOT TOUCH ANY LENS SURFACES WHILE HAN-

DLING THE MICROSCOPE; THIS MAY ADVERSLEY AF-

FECT IMAGE QUALITY.

C. FEATURES AND DEFINITIONS

EYEPEICE:

10X wide eld eyepiece with built in calibrated pointer for

measurement.

HEAD:

monocular head: 360° rotatable monocular head.

Binocular head: Dual view head allows 2 people to observe

the microscope simultaneously.

OBJECTIVES:

The lower functional component of the optical system. Avail-

able in 4X, 10X and 40XR, 100XR.

COAXIAL FOCUSING KNOBS:

The microscope is equipped with coaxial coarse (larger con-

trol) and ne focus (smaller control) knobs. Coaxial ne and

coarse adjustment enable you to perform two functions with-

out having to move your hands to a di󰀨erent position. The

reduction system allows for movement on a single ball bear-

ing dove-way for smooth and accurate focusing control. One

complete turn of the ne focus control is equal to a 1.8mm

vertical displacement of the stage.

CONDENSER:

The microscope is equipped with a 1.25 N.A. Abbe condenser

that has rack & pinion focusing, Iris Diaphragm, lter holder.

DIAPHRAGM:

The microscope is equipped with Iris Diaphragm. Its function

is to limit the amount of light transmitted to the objective for

preventing oversaturation, for which allows the amount of am-

bient light reaching the objective to be controlled to ensure the

high image e󰀨ect.

ILLUMINATOR:

LED illumination with intensity control, guarantees a clear and

- 2 -

crisp, cool-to-touch white light that lasts up to 60x longer than

traditional tungsten bulbs.

STAGE:

The stage of the microscope is equipped with built-in double

layer mechanical stage.

SAFETY RACK STOP:

Controls the maximum upward movement of the stage.

SPECIFICATIONS (With 10x Eyepiece)

Objective Total Magnication Field of View N.A.

4X 40X 4.5mm 0.10

10X 100X 1.8mm 0.25

40X 400X 0.45mm 0.65

100X Oil 1000X 0.18mm 1.25

D. OPERATING PROCEDURES

I. Microscope without an 1.25 Abbe-condenser

1. Select a position to work where little direct light falls on the

instrument. Avoiding placing the microscope near or facing

a large window as direct light may fall directly on the mi-

croscope and adversely a󰀨ect the contrast and resolution.

2. Plug it into any grounded receptacle.

3. Adjust the Iris diaphragm to control the amount of light

align with the in-stage condenser.

- 3 -

4. Place a low powered objective into position.

5. Place the micro-slide specimen to be observed under the

spring stage clips. If using a mechanical stage, pull back

the lever on the left side of the stage, insert the slide, then

bring the crescent shaped holder into contact with the slide.

Be certain that the cover slip of the slide is facing towards

the objective; otherwise you will not be able to focus your

specimen at high magnications.

6. Position the specimen so that it is centered over the in-

stage condenser.

7. Focus the objective on your specimen by turning the

LARGE COARSE ADJUSTMENT KNOBS until the image

of your specimen is bright and clear. Microscopists will al-

ways lower the objective to a point that they know is be-

neath the focal plane and focus upwards. Now you can

bring the specimen into sharp focus by turning the SMALL-

ER FINE FOCUS KNOBS.

8. With the specimen now in sharp focus, rotate the nose-

piece to the other objectives and focus using only the ne

focus knobs. Since the optics on the Microscopes are

Par-focal and Par-centered only a slight turn of the ne

focus knobs with be necessary.

NOTE: It is important to note that because of our built – in

stop the 4X and 10X objectives can never come into con-

tact with your microscope slides. The 40XR and 100XR may

occasionally touch the micro-slide but because these objec-

tives have retractable mounts your slide will not be damaged.

9. Adjust the Iris diaphragm until proper specimen contrast is

achieved.

10.You are now ready for microscope observation.

- 4 -

II. Microscope equipped with an 1.25 Abbe-condens-

er with or without the 100XR oil immersion objective.

1. Follow steps 1 through 6 and then:

2. FOCUSING THE CONDENSER. The sub-stage Abbe

condenser is mounted beneath the stage in a rack & pinion

focusing mount. Adjust the Abbe condenser by turning the

knob until the illumination of the eld is uniform.

3. Adjust the condenser iris diaphragm to match the N.A. of

the objective. This is done by rst closing the iris and open-

ing it slowly until the entire led is evenly and brightly lit

and in good focus. If the objective is changed to a higher

power, the iris must be adjusted to the new objective.

4. THE USE OF THE 100XR, OIL IMMERSION IS RE-

QUIRED FOR 100xR OBJECTIVES. Here is how to do it:

5. Lower the stage and spring out the objective to give your-

self room to work with. Place one or two drops of quality

immersion oil over the slide cover slip, which is needed

to gather enough light for viewing with 100xR. Spring the

100xR objective back in place again, and proceed by mov-

ing the stage up until the lens makes contact with the oil.

Continue (slowly) to focus down with coarse adjustment

until the color or a blurred outline of the specimen appears.

Now complete the focusing with the ne adjustment so that

your image details come into sharper focus.

6. When you are nished using the slides, ensure to clean

the oil o󰀨 of the slides and microscope lens by using lens

paper and cleaning solution.

7. In using the 100xR DIN objective, the most favourable res-

olution is obtained with the Abbe condenser nearly touch-

ing the slide specimen. Ideally, a drop of immersion oil is

placed between the condenser and the slide, as well as

between the slide and the 100xR DIN objective. Although

this practice is not always followed in routine study, it is the

only way to take full advantage of the inherent resolution

of the 1.25 NA Abbe condenser.

- 5 -

E. MAINTENANCE & CARE

Cleaning of the optical surfaces

Never take objectives or eyepieces apart. They should be

cleaned on the instrument since they are not easily removed.

To clean lens surfaces, rst remove dust using a soft brush

or blow o󰀨 with a small syringe. Use a cotton-tip applicator

and a small amount of xylene. Clean only the front lens ele-

ment of the objective and the top lens of the eyepiece. Wipe

again with a clean cotton-tip and, nally, blow or brush o󰀨 the

lens surfaces. The mirror or illuminator lens surfaces may

be cleaned in the same manner, but better results may be

obtained by wiping with a soft lint free cloth.

Cleaning and Lubricating of Mechanical parts

This type of maintenance should be done by an authorized

technician and will help insure many years of trouble free

use of your microscope.

Stage Elevation Caution - The stage is set at a standard

position for this particular optical system. Please exercise

caution if you need to elevate the stage. If the stage is raised

too high, the objective lens and the slide can easily be dam-

aged due to unintended contact, which could result in abra-

sions to each surface.

Empty Magnication

Keep in mind that there is a limit to meaningful magnication.

When you magnify an object beyond 1000x, it will continue

to get larger, but the resolution will stay the same. This will

actually make the image blurry and unclear, and it is called

empty magnication.

This is why it is not professionally recommended to change

the WF 10x eyepiece to a higher magnication eyepiece

such as the (WF 15x, 20x, or 25x), because 1000x magni-

cation is the maximum limit of clear magnication achieved

with a microscope.

- 6 -

F. ELECTRICAL MAINTANENCE

Charging/Re-charging NiMH rechargeable batteries.

(Rechargeable models only)

Your microscope is equipped with rechargeable Nickel Metal

Hydride (NiMH) batteries. Batteries should be charged prior

to rst use. Fully charge batteries, approximately 8 hours, by

plugging the supplied charger into a standard 110v outlet, and

attaching to the power inlet on the microscope.

You may also use the microscope while charging. Each com-

plete charge should give between 50-70 hours of use. Re-

chargeable batteries should give up to 500 recharge cycles,

depending on conditions. LED lamps will last a minimum of

10,000 hours under normal conditions.

If for any reason the light on your microscope does not appear

to be working, check the following:

1. Adjust the light intensity

switch to ensure that it is

not turned all the way down.

2. Check the Fuse – check to

ensure the fuse is not bro-

ken or burnt out.

- 7 -

WARNING

DISCONNECT POWER SUPPLY CORD FROM WALL

RECEPTACLE BEFORE REPLACING LAMP OR FUSE

Part No. LBB13

Rechargeable Battery Replacement

(Rechargeable models only)

1. Lay the microscope on its side on a at surface, and un-

screw the four rubber feet

2. Remove the base and lay on a at surface

3. Unscrew the two screws on both ends of the rectangular

plastic box

4. Remove the cover and replace the NiMH rechargeable

batteries

LED or Tungsten Halogen Lighting Replacement

(LED Models)

1. Lay the microscope on its side on a at surface

2. Ensure your microscope has been unplugged

3. Loosen the thumbscrew on the lamp housing cover and open

4. Remove the plastic pins on each side of the disc shaped

bulb with small pliers. Carefully remove the bulb by sliding

it out from under the metal tabs

CAUTION:

- Avoid contact with ngers as oils from skin can lead to break-

age or shorten the life of the lamp

- Do not look directly into the operating lamp for any period of

time; this may cause eye injury

- Halogen lamps operate at high temperatures that can cause

serious physical injuries

FUSE REPLACEMENT

The fuse holder is located on the back of the

microscope base. Unscrew the fuse holder

cap, remove fuse, and replace with a new

fuse rated 1.0 amp

- 8 -

Part No. FU102

G. TROUBLE SHOOTING

Objectives

If you are using any type of liquid on the slides and then view

through the microscope, make sure that you clean the ob-

jectives immediately after you are done. You can clean with

paper towel and xylene. If you do not clean after contact with

liquid the objective lens will become very dirty. This will greatly

a󰀨ect the viewing quality of the objective.

The eyepiece

If view is hindered, check for ngerprints or scratches on the

eyepiece. Do not scratch eyepiece with your ngernail, as

this will remove the optic coating. Only clean the outer lens

and do not attempt to take eyepiece apart. Clean by using a

can of compressed air meant for dusting, or breathe on lens

and wipe with lens paper.

Rack Stop Screw

This screw sets how far up the stage will move. Students

have a tendency to screw this all the way down. This limits the

upper movement of the stage, preventing objects from com-

ing into focus. Turn the screw back up. The more the screw

comes up the higher the stage is able to move upwards. Do

not turn the screw to far as the stage may hit the 40x and

100x objectives. When properly adjusted, the safety rack stop

prevents the objective lens from hitting the microscope which

can result in damage to the specimen slide.

Slide and Cover slip

If the slide does not come into focus check to see that the

slide is not upside down. Make sure the cover slide is facing

up. Also make sure the slide is not dirty; spots will show up if it

is. Also make sure that the cover slip over the specimen is not

too thick, use a 0.17mm thick cover slip over your specimen

- 9 -

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