STEINDORFF S-271 Manuale utente
Biological Microscope
Model: S-271/ S-273
Instruction Manual
S-273S-271
To ensure safety and obtain optimum performance and familiarize yourself with the use of this
microscope, we recommend that you read this manual thoroughly before operating the microscope.
Retain this instruction manual in an easily accessible place near the microscope for further reference.
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Contents
Safety notice .............................................................................. 2
1. Structure ................................................................................ 5
2. Application ............................................................................ 6
3. Technical parameters ............................................................. 7
4. Nomenclature ........................................................................ 8
5. Configuration ......................................................................... 9
6. Assemblage .......................................................................... 10
7. Operation ............................................................................. 13
8. Trouble Shooting ................................................................. 17
9. Care and Maintenance ......................................................... 19
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Safety notice
1. Transportation
As microscope is a precision instrument, handle with
care, avoiding impact or abrupt movement during
transportation. Do not push or pull the microscope
during using, otherwise the precision for image will be
reduced.
1_Hold the curve and keep the microscope in balance
2_Do not hold the focusing knob, eyepiece tubes and
stage as these parts are movable. Troubles maybe
caused by such handles.
Do not make specimen or filters fall off.
2. Working Environment
As microscope is a precision instrument, improper using will make it unworkable or reduce
its precision.
1_Do not expose the microscope in the sun directly
2_Temperature range is 0_-40_ and the max. humidity is 85%
3_Avoid high temperature and humidification otherwise there will be fog or mold on the lens
4_Avoid violent vibration as the vibration will reduce the image_s quality
5_Place microscope on a stable plane and keep it in balance. Keep the environment breezy
and cover the microscope with the dust-cover
6_Do not place microscope in moist room to avoid short circuit. Please turn off the power
supply as soon as water drop in the microscope. If there are other things unsafe come into
the microscope may cause short circuit too, please stop using and contact with manufacturers
3. Focusing knob
Never turn the left and right focusing knob in the
adverse direction at the same time. Do not turn the
coarse focusing knob when the stage reaches max.
position. These improper operations will make
focusing structure unworkable.
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4. Clamp
The clamps fix the microscope firmly during
transportation.
Please remove them before using.
5. Do not take the microscope apart
As it will be damaged. Such operations might have bad effect on the performance and make
user get an electric shock or injured. Please contact with manufacturers if there is any problem.
6. Make sure the voltage
There is correct input voltage in the label on
microscope. Make sure the voltage in your position
is right. Improper input voltage may cause short
circuit and fire. The microscope will be damaged.
Input voltage in the label: 100-240V 50/60Hz.
7. Use the spare parts supplied by the manufactures
The bulb, fuse and electronic cord have been assembled already in the factory and please make sure
to use the spare parts of them supplied by the manufacturers. Improper bulb, fuse and electronic
cord will destroy microscope and cause fire. Please make sure to use PE electronic cord when using
extra-long electronic cord.
Spare bulbs: 3W LED lamp
Spare fuse: 250V 1A delayed model, 5 X 20 mini type fuse
8. Mounting the bulb
Never touch the surface of bulb with your hand directly. Please use gloves or cloth material
when you mount the bulb to avoid leaving fingerprints. Fingerprints or stains should be wiped
off with a tissue moistened with a small amount of alcohol. The fingerprints will etch on the
bulb's surface and make brightness lower and life cycle shorter.
Please make sure the bulb's touching points are Ok as it will not light or short circuit if the
bulb's touching points are damaged. Insert the bulb's legs into the bulb holder firmly. The
bulb will go out if its legs have not been inserted into the holder firmly. Such Operation may
cause short circuit or smoking. At last, please make sure if the collector has been mounted
properly.
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9. Temperature for illumination
When you use the halogen bulb, there will be high temperature when the bulb is lightened, never
move the collector when the bulb is lightened. Do not touch the bulb within 30 minutes after it has
been turned off. Make sure the bulb has been be cooling enough time (at least 30 minutes) to avoid
scald.
Do not put fibre, papers and incendive things (eg. Gasoline, aether, methanol and ethanol) close to
the bulb.
10. Make sure to turn off the power supply before assembling
microscope, replacing bulb or fuse.
11.Observing with oil immersion
Using small amount of oil immersion is enough. The redundant oil will adhere to stage or
condenser and these will reduce microscope’s performance. Get rid of the redundant oil or clean the
lens by using aether or pure alcohol according to this instruction. Pay attention during process as
these things are incentive. The instruction you buy may not including some products mentioned in
this instruction. Safety has been considered during design while users still have the possibility to be
injured and the instruction to be damaged when improper operation made. Please pay much more
attention to read this instruction carefully before using and keep it properly to make sure it will be
got when it is necessary.
13.Operating the microscope only indoor and under the
altitude 2000m, IP52 grads.
If the equipment is used in manner not specified by the manufacturer, the provided by the
equipment may be impaired.
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1. Structure
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2. Application
S-271 / S-273 biological microscope are designed for clinical experiments and routine medical
examination as well as for teaching and research purpose in biology, pharmaceutical, and bacteriology
observation in medical and hygienic establishments, chemical laboratory, research institute and
college, ect. This product, with modern design, good -looking appearance, adopts many advanced
structure &technology in order that the clients can operate the instrument conveniently and safely.
There and also following accessories can be optional for the customer: darkfield, phase contrast,
photographic and video accessories.
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3. Technical parameters
1). Specification
1. Optical system:
Infinite Optical System
2. Illumination:
inserted transmitted illumination, 3W LED bulb(standard outfit)
Input voltage: 100V~240V 50/60Hz
Voltage undulation 10%
Rating 100V~240V 50/60Hz 0.8A
Fuse 250V 1A delayed model, 5X20 mini type fuse 2pcs
3. Focusing adjustment:
Division of fine focusing adjustment: 0.002mm
Fine focusing knob control range: stage will be up or down 0.2mm per circle
Coarse focusing knob control range: stage will be up or down 20mm
4. Mechanical stage
X-movement range: 40mm, Y-movement range: 75mm
5. Nosepiece:
Roller bear Quadruple nosepiece
6. Condenser:
Abbe NA=1.25 with iris diaphragm
7. Eye tubes:
Interpupillary range 48~75mm
8. Working environment:
Temperature: 0~40°
Humidity: max. 85%, no dew
2) Optical parameter
1, Objectives: Infinite plan achromatic objectives
Magnification N.A. Cover glass thinkness(mm) W.D. (mm) Dry/Oil
4X 0.1 0.17 15.5 Dry
10X 0.25 0.17 5.02 Dry
40X 0.65 0.17 0.60 Dry
100X 1.25 0.17 0.13 Oil
2, Eyepiece
WF10X/18mm
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4. Nomenclature
1)Total magnification
Total magnification = Eyepiece’s magnification X objective’s magnification
2)Numerical aperture (N.A.)
N.A. value will affect resolution and image’s brightness, it is the leading parameter for
objectives. N.A.= n X sina
n stands for refractive index of the medium between objective and specimen or condenser (air
or oil immersion)
a stands for half angle of max. aperture angle in the axis
The image will be sharper and brighter when the N.A. value is bigger
3)Resolution
Resolution can be measured by the distance value from one point to another one which could
be distinguished on the object surface
Resolution=λ /(2XN.A.)
λstands for wavelength ( λ= 0.55um)
4)Working distance
The distance between the object surface to the first surface of optical system when you get
the sharpest image. The W.D. will become more short when the magnification for the
objective is bigger.
5)Eyepiece view field
The max diameter measured when the diaphragm is open. 10X/18 means the magnification is
10X and the max diameter of view field is 18mm
6)Effective view field
Liner view field which is observed on the object surface
Effective view field = eyepiece view field / objective’s magnification
7)Depth of field
Depth of field stands for depth of the space in which there is sharp image on the object surface.
Depth of field will be longer when the diaphragm reduces. Depth of field will be shorter when
the N.A. becomes bigger.
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5. Configuration
Item Content Piece
1 Microscope body ( including stage, nosepiece, coaxial coarse and fine
focusing adjustment,bracket for condenser, adjusting power supply, LED
bulb and normal collector)
1 set
2 Siedentopf binocular/trinocular head 1 pc
3 Condenser ( with iris diaphragm, without auxiliary lens) 1 pc
4 Objectives(infinite plan achromatic objectives)4X, 10X, 40X, 100X 1 set
5 WF10X eyepiece 2 pcs
6 Blue filter 1 pc
7 Electronic cord 1 pc
8 Immersion oil 1 bottle
9 Dust cover 1 pc
10 Instruction 1 pc
11 Wrench 2.5, 1.5 1 set
Optional
Item Content
1 Collector with iris diaphragm
2 Dark field condenser
3 WF10X eyepiece with reticle
4 WF16X eyepiece
5 12V 20W halogen illumination
6 Phase contrast attachment
7 Polarizing attachment
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