STEINDORFF S-271 Manuale utente

Biological Microscope

Model: S-271/ S-273

Instruction Manual

S-273S-271

To ensure safety and obtain optimum performance and familiarize yourself with the use of this

microscope, we recommend that you read this manual thoroughly before operating the microscope.

Retain this instruction manual in an easily accessible place near the microscope for further reference.

STEINDORFF®

Contents

Safety notice .............................................................................. 2

1. Structure ................................................................................ 5

2. Application ............................................................................ 6

3. Technical parameters ............................................................. 7

4. Nomenclature ........................................................................ 8

5. Configuration ......................................................................... 9

6. Assemblage .......................................................................... 10

7. Operation ............................................................................. 13

8. Trouble Shooting ................................................................. 17

9. Care and Maintenance ......................................................... 19

STEINDORFF®

Safety notice

1. Transportation

As microscope is a precision instrument, handle with

care, avoiding impact or abrupt movement during

transportation. Do not push or pull the microscope

during using, otherwise the precision for image will be

reduced.

1_Hold the curve and keep the microscope in balance

2_Do not hold the focusing knob, eyepiece tubes and

stage as these parts are movable. Troubles maybe

caused by such handles.

Do not make specimen or filters fall off.

2. Working Environment

As microscope is a precision instrument, improper using will make it unworkable or reduce

its precision.

1_Do not expose the microscope in the sun directly

2_Temperature range is 0_-40_ and the max. humidity is 85%

3_Avoid high temperature and humidification otherwise there will be fog or mold on the lens

4_Avoid violent vibration as the vibration will reduce the image_s quality

5_Place microscope on a stable plane and keep it in balance. Keep the environment breezy

and cover the microscope with the dust-cover

6_Do not place microscope in moist room to avoid short circuit. Please turn off the power

supply as soon as water drop in the microscope. If there are other things unsafe come into

the microscope may cause short circuit too, please stop using and contact with manufacturers

3. Focusing knob

Never turn the left and right focusing knob in the

adverse direction at the same time. Do not turn the

coarse focusing knob when the stage reaches max.

position. These improper operations will make

focusing structure unworkable.

STEINDORFF®

4. Clamp

The clamps fix the microscope firmly during

transportation.

Please remove them before using.

5. Do not take the microscope apart

As it will be damaged. Such operations might have bad effect on the performance and make

user get an electric shock or injured. Please contact with manufacturers if there is any problem.

6. Make sure the voltage

There is correct input voltage in the label on

microscope. Make sure the voltage in your position

is right. Improper input voltage may cause short

circuit and fire. The microscope will be damaged.

Input voltage in the label: 100-240V 50/60Hz.

7. Use the spare parts supplied by the manufactures

The bulb, fuse and electronic cord have been assembled already in the factory and please make sure

to use the spare parts of them supplied by the manufacturers. Improper bulb, fuse and electronic

cord will destroy microscope and cause fire. Please make sure to use PE electronic cord when using

extra-long electronic cord.

Spare bulbs: 3W LED lamp

Spare fuse: 250V 1A delayed model, 5 X 20 mini type fuse

8. Mounting the bulb

Never touch the surface of bulb with your hand directly. Please use gloves or cloth material

when you mount the bulb to avoid leaving fingerprints. Fingerprints or stains should be wiped

off with a tissue moistened with a small amount of alcohol. The fingerprints will etch on the

bulb's surface and make brightness lower and life cycle shorter.

Please make sure the bulb's touching points are Ok as it will not light or short circuit if the

bulb's touching points are damaged. Insert the bulb's legs into the bulb holder firmly. The

bulb will go out if its legs have not been inserted into the holder firmly. Such Operation may

cause short circuit or smoking. At last, please make sure if the collector has been mounted

properly.

STEINDORFF®

9. Temperature for illumination

When you use the halogen bulb, there will be high temperature when the bulb is lightened, never

move the collector when the bulb is lightened. Do not touch the bulb within 30 minutes after it has

been turned off. Make sure the bulb has been be cooling enough time (at least 30 minutes) to avoid

scald.

Do not put fibre, papers and incendive things (eg. Gasoline, aether, methanol and ethanol) close to

the bulb.

10. Make sure to turn off the power supply before assembling

microscope, replacing bulb or fuse.

11.Observing with oil immersion

Using small amount of oil immersion is enough. The redundant oil will adhere to stage or

condenser and these will reduce microscope’s performance. Get rid of the redundant oil or clean the

lens by using aether or pure alcohol according to this instruction. Pay attention during process as

these things are incentive. The instruction you buy may not including some products mentioned in

this instruction. Safety has been considered during design while users still have the possibility to be

injured and the instruction to be damaged when improper operation made. Please pay much more

attention to read this instruction carefully before using and keep it properly to make sure it will be

got when it is necessary.

13.Operating the microscope only indoor and under the

altitude 2000m, IP52 grads.

If the equipment is used in manner not specified by the manufacturer, the provided by the

equipment may be impaired.

STEINDORFF®

1. Structure

STEINDORFF®

2. Application

S-271 / S-273 biological microscope are designed for clinical experiments and routine medical

examination as well as for teaching and research purpose in biology, pharmaceutical, and bacteriology

observation in medical and hygienic establishments, chemical laboratory, research institute and

college, ect. This product, with modern design, good -looking appearance, adopts many advanced

structure &technology in order that the clients can operate the instrument conveniently and safely.

There and also following accessories can be optional for the customer: darkfield, phase contrast,

photographic and video accessories.

STEINDORFF®

3. Technical parameters

1). Specification

1. Optical system:

Infinite Optical System

2. Illumination:

inserted transmitted illumination, 3W LED bulb(standard outfit)

Input voltage: 100V~240V 50/60Hz

Voltage undulation 10%

Rating 100V~240V 50/60Hz 0.8A

Fuse 250V 1A delayed model, 5X20 mini type fuse 2pcs

3. Focusing adjustment:

Division of fine focusing adjustment: 0.002mm

Fine focusing knob control range: stage will be up or down 0.2mm per circle

Coarse focusing knob control range: stage will be up or down 20mm

󶂢

4. Mechanical stage

X-movement range: 40mm, Y-movement range: 75mm

5. Nosepiece:

Roller bear Quadruple nosepiece

6. Condenser:

Abbe NA=1.25 with iris diaphragm

7. Eye tubes:

Interpupillary range 48~75mm

8. Working environment:

Temperature: 0~40°

Humidity: max. 85%, no dew

2) Optical parameter

1, Objectives: Infinite plan achromatic objectives

Magnification N.A. Cover glass thinkness(mm) W.D. (mm) Dry/Oil

4X 0.1 0.17 15.5 Dry

10X 0.25 0.17 5.02 Dry

40X 0.65 0.17 0.60 Dry

100X 1.25 0.17 0.13 Oil

2, Eyepiece

WF10X/18mm

STEINDORFF®

4. Nomenclature

1)Total magnification

Total magnification = Eyepiece’s magnification X objective’s magnification

2)Numerical aperture (N.A.)

N.A. value will affect resolution and image’s brightness, it is the leading parameter for

objectives. N.A.= n X sina

n stands for refractive index of the medium between objective and specimen or condenser (air

or oil immersion)

a stands for half angle of max. aperture angle in the axis

The image will be sharper and brighter when the N.A. value is bigger

3)Resolution

Resolution can be measured by the distance value from one point to another one which could

be distinguished on the object surface

Resolution=λ /(2XN.A.)

λstands for wavelength ( λ= 0.55um)

4)Working distance

The distance between the object surface to the first surface of optical system when you get

the sharpest image. The W.D. will become more short when the magnification for the

objective is bigger.

5)Eyepiece view field

The max diameter measured when the diaphragm is open. 10X/18 means the magnification is

10X and the max diameter of view field is 18mm

6)Effective view field

Liner view field which is observed on the object surface

Effective view field = eyepiece view field / objective’s magnification

7)Depth of field

Depth of field stands for depth of the space in which there is sharp image on the object surface.

Depth of field will be longer when the diaphragm reduces. Depth of field will be shorter when

the N.A. becomes bigger.

STEINDORFF®

5. Configuration

Item Content Piece

1 Microscope body ( including stage, nosepiece, coaxial coarse and fine

focusing adjustment,bracket for condenser, adjusting power supply, LED

bulb and normal collector)

1 set

2 Siedentopf binocular/trinocular head 1 pc

3 Condenser ( with iris diaphragm, without auxiliary lens) 1 pc

4 Objectives(infinite plan achromatic objectives)4X, 10X, 40X, 100X 1 set

5 WF10X eyepiece 2 pcs

6 Blue filter 1 pc

7 Electronic cord 1 pc

8 Immersion oil 1 bottle

9 Dust cover 1 pc

10 Instruction 1 pc

11 Wrench 2.5, 1.5 1 set

Optional

Item Content

1 Collector with iris diaphragm

2 Dark field condenser

3 WF10X eyepiece with reticle

4 WF16X eyepiece

5 12V 20W halogen illumination

6 Phase contrast attachment

7 Polarizing attachment

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