Turner BioSystems GloRunner Manuale utente
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GloRunner™ Microplate Luminometer
Operating Manual
Dated: 09/18/2006
Version: 1.2
Part Number: 998-9005
645 N. Mary Avenue ŸSunnyvale CA 94085
Phone: 408.636.2400 ŸToll Free: 888.636.2401
Fax: 408.737.7919 Ÿwww.turnerbiosystems.com
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Table of Contents
I. Introduction 4
Description 4
Inspection 4
General Precautions 4
II. Hardware Overview 5
Functions and Features 5
III. Setting up the GloRunner™ Microplate Luminometer 7
Procedure 7
IV. GloRunner™ Microplate Luminometer DXL Software 7
Overview 7
Software Operation 9
Communications Settings Procedure 11
GloRunner™ User Interface Features 13
Setting the Options 14
V. Reading Samples 16
Starting a Run 16
VI. Calibration of the GloRunner17
Procedure 17
VII. Relative Light Units and Common Questions 19
Overview 19
FAQs: 19
APPENDIX A - Installing the Software 21
Overview 21
Procedure 21
APPENDIX B - LED Messages and Firmware Upgrades 23
LED Lights 23
Firmware Upgrades 23
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APPENDIX C - Maintenance, Warranty, & Service 24
Maintenance 24
Warranty 24
Obtaining Service 25
Warranty Service 25
Out-of-Warranty Service 26
Address for Shipment: Error! Bookmark not defined.
APPENDIX D – Specifications 27
Specifications for the GloRunnerMicroplate Luminometer 27
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I. Introduction
Description
The GloRunneris an easy-to-use, sensitive luminometer with a broad
dynamic range designed to read glow luminescent reactions in 96-well
plates. The instrument’s small size and affordable price make it an
ideal laboratory instrument. This manual is designed to guide you
through the installation, setup, calibration, and operation of your
instrument.
Inspection
Upon receiving your luminometer, please inspect it carefully and make
sure all accessories are present (refer to the packing list shipped with
the instrument).
Standard accessories:
vPower Cord
v9-pin Serial Cable
vOperating Manual
vQuick Start Guide
vSoftware
General Precautions
vThis instrument is intended for indoor use only.
vA clear, transparent adhesive film should be placed over the 96-
well microplate to prevent spills or cross-contamination between
wells.1
vWipe up spills immediately. See Appendix C for cleaning
instructions.
vThe luminometer contains sensitive optical components and
precision-aligned mechanical assemblies. Avoid rough handling.
1This adhesive film may be purchased at Rainin, catalog no. 96-SP-100
or any similar manufacturer.
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II. Hardware Overview
Functions and Features
This section describes the various hardware features of the GloRunner, with
Figures 1-3 as references.
vLED Status Lights: These lights report the status of the
instrument. The green LED indicates the unit is on and ready. A
solid yellow LED indicates the instrument is not communicating
with the host computer. The red LED indicates a potential
component failure within the instrument. See Appendix B for a
detailed description of what the lights represent.
vSample Tray: Sample plates are placed on the tray for reading.
The plate format for this version is 96-well. No other well format
can be read.
vDrawer: The drawer opens to receive sample plate.
vPower Switch: The ON/OFF switch is located on the back panel
of the instrument.
vPower Cord Connection: The power cord connects into the rear
panel of the instrument.
vRS-232 Serial Port Connection: The 9-pin serial port is used to
connect the GloRunnerto a computer via the RS-232 cable.
vRS-232 Cable
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Power Cord
Connection
Power
Switch
RS-232 Serial
Port
Connection
Figure 3
LED Status
Lights
Sample Tray
Figure 1
9-pin Male Connector
(To
GloRunner
)
Figure 2
9-pin Female Connector
(To Computer)
Drawer
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III. Setting up the GloRunner™ Microplate Luminometer
Procedure
1. Position the instrument on a flat surface. Leave enough room for the sample
tray to extend in front of the unit (approximately 7 inches).
2. Plug in the unit. In some countries, the power plug provided may not match
the wall connection. If this is the case, you can use the appropriate power
cord if the input power is between 100 and 240 volts AC, and the nominal
frequency is between 50 and 60 Hz.
3. From your own computer, install the software according to the instructions
in Appendix A.
4. Connect the 9-pin serial cable between the luminometer and the computer.
The male 9-pin connector attaches to the GloRunnerand the female 9-pin
connector attaches to your computer (See Figure 2).
5. Turn on the power switch (rear of unit).
Note: The sample tray opens and closes via the software user interface. To
open the sample tray, please proceed to the next section for further
instructions.
IV. GloRunner™ Microplate Luminometer DXL Software
Overview
The GloRunnerMicroplate Luminometer DXL software is very easy to use.
This direct-to-Excel based software is designed to give the user flexibility in
data retrieval, storage, and manipulation while using the GloRunner
Microplate Luminometer. User-defined protocols can be stored as templates for
easy retrieval of stored settings. For added help while using the GloRunner
DXL software, a tutorial is available. You will see the following dialog box
after clicking on the GloRunnericon from your desktop (an alternate way of
accessing the GloRunnerDXL software is through the program manager):
Figure 4
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If you elect to review the tutorial, you will see the following tutorial window.
The tutorial can be printed by clicking the “Print” button.
When you are done reviewing the tutorial, click the “Close” button to close the
window.
Figure 5
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The following dialog box will appear after closing the tutorial, or if you elect not
to review the tutorial:
Figure 6
Software Operation
The GloRunnerDXL software is designed to run in conjunction with Excel.
When using the GloRunnerMicroplate Luminometer, you have two workbook
options. The first option is to create a new workbook for data collection. This
option should be selected when first using the GloRunner. The second option
is to use an existing workbook. This feature allows you to add data to an
existing workbook. This should be selected if you would like to keep a running
log of data from multiple assays.
If you are using the luminometer for the first time, select the “Create a new
workbook” option. Once this is selected you will be prompted to select a data
storage template format. We suggest using the “2DimensionalFormat” template.
The “2DimensionalFormatWithTimes” template can also be used if you are
interested in knowing well read times. Figure 7 shows the workbook selection
screen.
Figure 7
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After selecting the data storage format, the GloRunnerdialog box will open
along with the active Excel spreadsheet. You should see the following on your
screen.
Note: These are two separate windows.
Figure 8
The top window is the GloRunnerdialog box. The bottom window is the
active Excel spreadsheet in which data from the run will appear. The dialog box
is used for starting runs, changing or selecting options, toggling the tray
position, and establishing communication between the GloRunnerand the
computer. If communication has not already been established between the
GloRunnerand the computer, this should be done now.
Indice
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