UNISENSE OXYGEN MICROOPTODE Manuale utente
OXYGEN MICROOPTODE USER MANUAL
Oxygen MicrOOptOde user Manual
Copyright © 2013 · Unisense A/S
Version August 2013
OXYGEN MICROOPTODE USER MANUAL
UNISENSE A/S
TABLE OF CONTENTS
WARRANTY AND LIABILITY...................................................4
CONGRATULATIONS WITH YOUR NEW PRODUCT! ............................5
Support, ordering, and contact information 5
OVERVIEW ...................................................................7
GETTING STARTED ...........................................................8
unpacking anew microoptode 8
connecting the microoptode 8
MEASUREMENTS............................................................11
handling of microoptodeS 11
interference 12
STORAGE AND MAINTENANCE ..............................................13
Storage 13
cleaning the SenSor 13
APPENDIX: EQUILIBRIUM O2CONCENTRATIONS .............................15
6
NOTICE TO PURCHASER
This product is for research use only. Not for use in human diagnostic or
therapeutic procedures.
WARNING
Microsensors have very pointed tips and must be handled with care
to avoid personal injury and only by trained personnel.Unisense A/S
recommends users to attend instruction courses to ensure proper use of
the products.
WARRANTY AND LIABILITY
The oxygen MicroOptode Sensor is covered by a 1 year limited warranty
or 1 million data points (estimated 300 hours of measurements).
Wear-off of dye resulting in reduced signal quality, e.g. from profiling
into biological matrices like sediments,and bend and breakage of the
fiber optic cable is not covered by the warranty.
MicroOptodes are consumables. Unisense will only replace
dysfunctional sensors if they have been tested in accordance with the
instructions in the manual within 14 days of receipt of the sensor(s).
The warranty does not include repair or replacement necessitated by
accident, neglect, misuse, unauthorized repair, or modification of the
product. In no event will Unisense A/S be liable for any direct, indirect,
consequential or incidental damages, including lost profits, or for any
claim by any third party, arising out of the use, the results of use, or the
inability to use this product.
Unisense mechanical and electronic laboratory instruments must
only be used under normal laboratory conditions and a dry and clean
environment. Unisense assumes no liability for damages on laboratory
instruments due to unintended field use or exposure to dust, humidity
or corrosive environments.
REPAIR OR ADJUSTMENT
Sensors and electrodes cannot be repaired. Equipment that is not
covered by the warranty will, if possible, be repaired by Unisense A/S
with appropriate charges paid by the customer. In case of return of
equipment please contact us for return authorization.
For further information please see the document General Terms of Sale
and Delivery of Unisense A/S as well as the manuals for the respective
products.
WARRANTY AND LIABILITY
7
suppOrt, Ordering, and cOntact infOrMatiOn
The Unisense MicroOptode is an oxygen measuring system based
on the latest development within optical fiber technology. Use
the MicroOptode for fast and accurate oxygen measurement in a
broad variety of research applications.
If you wish to order additional products or if you encounter any
problems and need scientific/technical assistance, please do not
hesitate to contact our sales and support team. We will respond to
your inquiry within one working day.
E-mail: [email protected]
Unisense A/S
Tueager 1
DK-8200 Aarhus N, Denmark
Tel: +45 8944 9500
Fax: +45 8944 9549
Further documentation and support is available at our website
www.unisense.com.
CONGRATULATIONS WITH YOUR NEW PRODUCT!
REPLACEMENT OF MICROOPTODES
Unisense will replace MicroOptodes that have been damaged during shipment provided that:
• The MicroOptodes were tested immediately upon receipt in accordance with the delivery
note and the manual.
• The white seal is still intact
• The MicroOptodes are returned to Unisense for inspection within two weeks
• The MicroOptodes are correctly packed for return to Unisense, in accordance with the
note included in the MicroOptode shipping box.
88
RECOMMENDED METERS
Unisense MicroOptode Meter
999
OVERVIEW
This manual covers all the Unisense MicroOptodes. The
MicroOptode relies on the latest developments within optical fiber
technology utilizing near infrared dyes for improved performance,
high precision, high reliability, low cross-interference and fast
response times.
The MicroOptodes are designed for research applications within
physiology, biotechnology, environmental sciences and related
areas.
meaSuring principle
The very tip of the optical fiber is coated with a specialized
dye that is excitable with red light (610-630 nm). When the
MicroOptode is connected to a Unisense MicroOptode Meter
the dye is excited with sinusoidal modulated red excitation light.
The dye show an oxygen-dependent luminescence in the near
infrared (NIR, 760-790 nm), and by detecting quenching of the
luminescence the oxygen concentration can be determined in the
sample .
WARNING
Unisense
MicroOptodes are
neither intended
nor approved for
use on humans
Measuring principle:
The near infrared dye
shows oxygen dependent
luminescence in the
near infrared (NIR) after
exposure to excitation
light. A) High NIR
emission at low oxygen
concentration and B)
low NIR at high oxygen
concentration.
10
WARNING
Do not remove the
seal and protective
plastic tube
before these steps
and calibration
are successfully
completed.
GETTING STARTED
unpacking anew MicrOOptOde
The MicroOptode sensor is shipped in a protective tube with the
optic fiber positioned half exposed in the needle. Please do not
remove the white seal or move the fiber further out of the needle
before the calibration procedure has been successfully completed.
cOnnecting the MicrOOptOde
Remove the black cap from the MicroOptode connector and
remove the red cap from the MicroOptode Meter. Insert the male
fiber plug of the MicroOptode cable into the ST-receptable of
the MicroOptode Meter and turn the bayonet coupling gently
clockwise until the plug is locked firmly.
calibratiOn
Please consult the software manual for instructions on how to
calibrate the MicroOptode in the software.
Calibration of the oxygen MicroOptode sensor is performed as a
two-point-calibration by measuring the MicroOptode response
in a solution saturated with oxygen and an anoxic solution.
Remember that a temperature sensor is required for correct
oxygen measurements unless the measurement and calibration is
completed at stable temperature.
oxygen Saturated Solution
Place the MicroOptode in a well-aerated calibration solution
(e.g. by bubbling with air in the Unisense calibration chamber).
The MicroOptode and the connecting male fiber plug.
Indice
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