VWR Peqlab Cast-It Manuale utente

Instruction Manual – Bedienungsanleitung

Cast-It™ Gel CASTER

Cast-It™ Gelgießstand

Instruction Manual Cast-It™ Gel CASTER

VWR v0617 E

CONTENT

WARRANTY 1

PACKAGING LIST 1

ASSEMBLING GEL PLATES FOR CASTING 2

BUFFERS AND SOLUTIONS 3

TROUBLESHOOTING 4

TECHNICAL SUPPORT AND ORDERING INFORMATION 5

Instruction Manual Cast-It™ Gel CASTER

VWR v0617 E 1

WARRANTY

VWR guarantees that the system you have received has been thoroughly tested and meets its published

specification.

However, immediately upon arrival, please check carefully that the shipment is complete and has not

been damaged during transit. For missing parts or to report any kind of damage, please contact VWR

(see 'TECHNICAL SUPPORT AND ORDERING INFORMATIONS'). Please retain all packaging materials

until the delivery has been completely checked since this will speed up the return of goods if required

and reduce environmental impact. Any form of returns, replacements or credit notes must be agreed in

advance by VWR.

For the complete range of PerfectBlue

™

electrophoresis and blotting systems, VWR guarantees a

warranty period of 36 months if the products have been used solely according to the instruction

manual, if not agreed differently. No liability is accepted for loss or damage arising from incorrect use.

VWR's liability is limited to the repair or replacement of the unit or refund of the purchase price, at

VWR's discretion. VWR is not liable for any consequential damages. After the warranty period has

expired VWR can offer repairs at low costs.

VWR reserves the right to alter the technical specifications of the PerfectBlue

™

electrophoresis or blotting

systems without prior notice. This will enable us to implement developments as soon as they arise.

PACKAGING LIST

Unless differently agreed or marked on the delivery note the following items are included in shipment

for the Cast-It™ Gel Caster:

Casting base

Center unit with side clamps

2 casting cams

2 gaskets for the center unit (red)

2 gaskets on the casting base (black)

When using the casting base the first time remove the plastic strips from the black gaskets.

Note: Glass plates, spacers and combs are not included in the shipment and should be ordered

separately if needed!

Instruction Manual Cast-It™ Gel CASTER

VWR v0617 E 2

ASSEMBLING GEL PLATES FOR CASTING

Picture 1: Invert casting base.

Picture 2: Place gel assembly.

Picture 3: Turn handles.

See Table 1 for volumes of acrylamide mixture needed. Pour gel solution slowly to avoid any

bubbles. When gel is poured place comb at top to create wells.

Remove gels from the Cast-It™ center unit and place them into the electrophoresis chamber.

Table 1:

Unit

Twin S

Twin M

Twin ExW S

Twin L

0.8 mm spacer

8 ml

18 ml

16 ml

32 ml

1.5 mm spacer

15 ml

34 ml

30 ml

60 ml



The center unit of the Cast

It™ is attached to the casting

base with safety cams and slot arrangement.

For casting gels remove the center unit from the base.

Invert casting base (picture 1).

Place the center of the Cast-It™ on the inverted casting

base. The notches of the center unit need to lock into

the four acrylic squares on the underside of the casting

base. Arrange a gel assembly including a notched

glass plate, side spacers and a blank glass plate. Place

the assembly onto one side of the center unit. Slide the

side clamps over the assembly and tighten the screws

(picture 2). DO NOT OVERTIGHTEN. Place a second

gel assembly on the other side. If you only want to cast

one gel, place a blank glass plate or a blocking plate,

on the other side. The bottom of the glass plates needs

to sit flat on the acrylic squares of the inverted casting

base to obtain a good seal. Remove the center unit and

turn around the casting base.

Wet silicone mats with e.g. water or buffer to prolong

their life span.

Place the center unit onto the casting base. Insert the

casting cams (with handles pointing up) into the holes

in the center unit. Simultaneously, turn cams one half

turn to tighten the assembly onto the gasket of the

casting base.

Once the glass plates are secured into the casting base

the gel may be poured. (An initial leak test using a

small amount of water is recommended - add 2 - 3 ml

of water and let stand for 2 minutes. If no leakage is

visible, remove water and proceed with gel casting. If

leakage occurs, take assembly apart and try again.)

Instruction Manual Cast-It™ Gel CASTER

VWR v0617 E 3

A few tips about caring for your system:

Clean all VWR acrylic systems with warm water and a mild detergent. Do not autoclave, bake or

microwave your unit. Temperatures over 50 °C can cause damage to the acrylic.

Always wet silicone mats with e.g. water or buffer before placing the center unit.

WARNING! DO NOT USE ETHANOL OR OTHER ORGANIC SOLVENTS TO CLEAN VWR PER-

FECTBLUE™ PRODUCTS. Organic solvents cause acrylic to 'craze' or crack.

BUFFERS AND SOLUTIONS

1 x Tris-Glycine electrophoresis buffer

25 mM Tris

250 mM glycine (electrophoresis grade) pH 8.3

0.1 % SDS

Prepare a 5 x stock solution of electrophoresis buffer by dissolving 15.1 g Tris base and 94 g glycine in

900 ml of deionized H

O. Add 50 ml of a 10 % stock solution of SDS (electrophoresis grade) and

adjust the volume to 1000 ml with H

Resolving Gels for Tris-Glycine SDS-PAGE

6 % 8 % 10 % 12 % 15 %

O 5.3 4.6 4.0 3.3 2.3

30 % acrylamide mix 2.0 2.7 3.3 4.0 5.0

1.5 mM Tris (pH 8.8) 2.5 2.5 2.5 2.5 2.5

10 % SDS 0.1 0.1 0.1 0.1 0.1

10 % ammonium persulfate 0.1 0.1 0.1 0.1 0.1

TEMED 0.008 0.006 0.004 0.004 0.004

All amounts in ml per 10 ml gel solution

5 % Stacking Gels for Tris-Glycine SDS-PAGE

1 ml 2 ml 3 ml 5 ml 10 ml

O 0.68 1.4 2.1 3.4 6.8

30 % acrylamide mix 0.17 0.33 0.5 0.83 1.7

1.0 mM Tris (pH 6.8) 0.13 0.25 0.38 0.63 1.25

10 % SDS 0.01 0.02 0.03 0.05 0.1

10 % ammonium persulfate 0.01 0.02 0.03 0.05 0.1

TEMED 0.001 0.002 0.003 0.005 0.01

All amounts in ml

Instruction Manual Cast-It™ Gel CASTER

VWR v0617 E 4

TROUBLESHOOTING

Below are some possible solutions to potential problems. If these suggestions are unsuccessful, do not

hesitate to contact us (see 'TECHNICAL SUPPORT AND ORDERING INFORMATIONS').

Problem Cause Solution

Leakage of gel

solution

Glass plates and spacers have

not been aligned correctly

For correct alignment of glass plate sandwich it is necessary

to place the upper buffer chamber on top of the inverted

casting base. The bottom of the glass plates and the spacers

need to sit flat on the acrylic squares.

Glass plates or spacers are

dirty

Clean thoroughly.

Uneven edges of glass plates

Only use glass plates with polished edges.

Silicone gaskets of casting

base are dirty

Clean thoroughly with a mild detergent and rinse with

distilled water, dry without leaving smearing, i.e. air dry with

a non-smearing paper or washleather.

Silicone gaskets of casting

base are brittle

Replace silicone gaskets which can be easily removed. New

gaskets will stick due to their own adherence. Also, wet

silicone mats with e.g. water or buffer before placing the

center unit onto the casting base to prolong their life span.

Skewed bands Gel has not polymerized

properly at wells.

De-gas gel solution before casting and increase APS and

TEMED concentrations. The comb can be wiped with TEMED

just prior to casting to improve polymerization.

Resolving gel is uneven at top.

Overlay gel carefully using water saturated n-butanol and

make sure casting stand is level.

The upper buffer chamber is

leaking either through the gel

or along the side spacers.

Check gel to make sure that it is a solid slab inside the glass

and check the setup of the apparatus to ensure a seal with

the gasket. Always use combs and spacers of the same

thickness.

'Frowning' of

outside lanes

Leakage of buffer along sides

or along

spacers inside the gel

assembly.

Do not move spacer after polymerisation of gel. Make sure

that the glass plates are pressed firmly against the gasket of

the UBC.

Silicone mats wear

out quickly

Sticky surface of the mats

adheres too strongly to the

glass plates. Upon removal of

the center unit silicone mats

are torn apart.

Wet the silicone mats with e.g. water or buffer before placing

UBC onto the casting base to prolong their life span.

Instruction Manual Cast-It™ Gel CASTER

VWR v0617 E 5

TECHNICAL SUPPORT AND ORDERING INFORMATION

For technical questions and more detailed information on VWR’s products please visit www.de.vwr.com

to find the respective contact person.

Power Supplies

Do not hesitate to contact us for advice on which Power Supply is most suitable for your application.

PerfectBlue™ Double Gel System Twin S

Item Description Cat. No.

Double gel system Twin S

system for gels 10 x 10 cm (W x L), without casting base PEQL45-1010-I

system for gels 10 x 10 cm (W x L), with casting base PEQL45-1010-C

Casting base S including silicone gaskets and cams PEQL45-1010-CB

Cast-It™ S gel caster complete system for sealing up to 2 gels PEQL45-1010-CU

Silicone gaskets 2 replacement silicone gaskets for Casting base S PEQL45-1010-CG

Replacement gaskets 2 replacement gaskets for upper buffer chamber S PEQL45-1010-GK

Side clamps 2 side clamps for fixation of glass plates PEQL45-1010-CL

Wing screws 2 wing screws for fixation of side clamps PEQL45-1010-WK

Notched glass plate clear, 10 x 10 x 0.24 cm PEQL45-1010-10R

Blank glass plate clear, 10 x 10 x 0.24 cm PEQL45-1010-10G

Notched alumina plate white, 10 x 10 x 0.10 cm PEQL45-1010-10RA

Standard combs 1.5 mm 6 teeth

175 µl* PEQL45-1010-6D

1.5 mm 8 teeth

120 µl* PEQL45-1010-8D

1.5 mm 10 teeth

90 µl* PEQL45-1010-10D

1.5 mm 12 teeth

70 µl* PEQL45-1010-12D

0.8 mm 6 teeth

95 µl* PEQL45-1010-6C

0.8 mm 8 teeth

65 µl* PEQL45-1010-8C

0.8 mm 10 teeth

50 µl* PEQL45-1010-10C

0.8 mm 12 teeth

35 µl* PEQL45-1010-12C

Preparative combs 1.5 mm 2 teeth

1490/105 µl* PEQL45-1010-PD

0.8 mm 2 teeth

795/56 µl* PEQL45-1010-PC

SpacerPlacer Facilitates the correct alignment of Spacers PEQL45-1010-SP

Spacer Sets

(2 side & 1 bottom spacer)

1.5 mm 0.7 cm wide

3 pieces PEQL45-1010-SD

0.8 mm 0.7 cm wide

3 pieces PEQL45-1010-SC

* volumes are calculated for a well length of 14 mm

Instruction Manual Cast-It™ Gel CASTER

VWR v0617 E 6

PerfectBlue™ Double Gel System Twin M

Item Description Cat. No.

Double gel system Twin M

system for gels 16 x 14 cm (W x L), without casting base PEQL45-1614-I

system for gels 16 x 14 cm (W x L), with casting base PEQL45-1614-C

Casting base M including silicone gaskets and cams PEQL45-1614-CB

Cast-It™ M gel caster complete system for sealing up to 2 gels PEQL45-1614-CU

Silicone gaskets 2 replacement silicone gaskets for Casting base M PEQL45-1614-CG

Replacement gaskets 2 replacement gaskets for upper buffer chamber M PEQL45-1614-GK

Side clamps 2 side clamps for fixation of glass plates PEQL45-1614-CL

Wing screws 2 wing screws for fixation of side clamps PEQL45-1010-WK

Notched glass plate clear, 16 x 14 x 0.32 cm PEQL45-1614-14R

Blank glass plate clear, 16 x 14 x 0.32 cm PEQL45-1614-14G

Standard combs 1.5 mm 10 teeth

234 µl* PEQL45-1614-10D

1.5 mm 15 teeth

140 µl* PEQL45-1614-15D

1.5 mm 20 teeth

90 µl* PEQL45-1614-20D

1.5 mm 24 teeth

65 µl* PEQL45-1614-24D

0.8 mm 10 teeth

125 µl* PEQL45-1614-10C

0.8 mm 15 teeth

75 µl* PEQL45-1614-15C

0.8 mm 20 teeth

50 µl* PEQL45-1614-20C

0.8 mm 24 teeth

35 µl* PEQL45-1614-24C

Preparative combs 1.5 mm 2 teeth

3570/150 µl* PEQL45-1614-PD

0.8 mm 2 teeth

1900/80 µl* PEQL45-1614-PC

SpacerPlacer Facilitates the correct alignment of Spacers PEQL45-1010-SP

Spacer Sets

(2 side & 1 bottom spacer)

1.5 mm 1.0 cm wide

3 pieces PEQL45-1614-SD

0.8 mm 1.0 cm wide

3 pieces PEQL45-1614-SC

* volumes are calculated for a well length of 20 mm

PerfectBlue™ Double Gel System Twin L

Item Description Cat. No.

Double gel system Twin L

system for gels 20 x 20 cm (W x L), without casting base PEQL45-2020-I

system for gels 20 x 20 cm (W x L), with casting base PEQL45-2020-C

Casting base L including silicone gaskets and cams PEQL45-2020-CB

Cast-It™ L gel caster complete system for sealing up to 2 gels PEQL45-2020-CU

Silicone gaskets 2 replacement silicone gaskets for Casting base L PEQL45-2020-CG

Replacement gaskets 2 replacement gaskets for upper buffer chamber L PEQL45-2020-GK

Side clamps 2 side clamps for fixation of glass plates PEQL45-2020-CL

Wing screws 2 wing screws for fixation of side clamps PEQL45-1010-WK

Notched glass plate clear, 20 x 20 x 0.32 cm PEQL45-2020-20R

Blank glass plate clear, 20 x 20 x 0.32 cm PEQL45-2020-20G

Standard combs 1.5 mm 10 teeth

305 µl* PEQL45-2020-10D

1.5 mm 15 teeth

185 µl* PEQL45-2020-15D

1.5 mm 20 teeth

125 µl* PEQL45-2020-20D

1.5 mm 25 teeth

90 µl* PEQL45-2020-25D

0.8 mm 10 teeth

165 µl* PEQL45-2020-10C

0.8 mm 15 teeth

100 µl* PEQL45-2020-15C

0.8 mm 20 teeth

65 µl* PEQL45-2020-20C

0.8 mm 25 teeth

50 µl* PEQL45-2020-25C

Preparative combs 1.5 mm 2 teeth

3330/110 µl* PEQL45-2020-PD

0.8 mm 2 teeth

1776/59 µl* PEQL45-2020-PC

SpacerPlacer Facilitates the correct alignment of Spacers PEQL45-1010-SP

Spacer Sets

(2 side & 1 bottom spacer)

1.5 mm 1.0 cm wide

3 pieces PEQL45-2020-SD

0.8 mm 1.0 cm wide

3 pieces PEQL45-2020-SC

* volumes are calculated for a well length of 20 mm

Instruction Manual Cast-It™ Gel CASTER

VWR v0617 E 7

PerfectBlue™ Double Gel System Twin ExW S

Item Description Cat. No.

Double gel system

Twin ExW S

system for gels 20 x 10 cm (W x L), without casting base PEQL45-2010-I

system for gels 20 x 10 cm (W x L), with casting base PEQL45-2010-C

Casting base ExW including silicone gaskets and cams PEQL45-2010-CB

Cast-It™ ExW S gel caster complete system for sealing up to 2 gels PEQL45-2010-CU

Silicone gaskets 2 replacement silicone gaskets for Casting base ExW S PEQL45-2010-CG

Replacement gaskets 2 replacement gaskets for upper buffer chamber ExW S PEQL45-2010-GK

Side clamps 2 side clamps for fixation of glass plates PEQL45-2010-CL

Wing screws 2 wing screws for fixation of side clamps PEQL45-1010-WK

Notched glass plate clear, 20 x 10 x 0.30 cm PEQL45-2010-10R

Blank glass plate clear, 20 x 10 x 0.30 cm PEQL45-2010-10G

Standard combs 1.5 mm 10 teeth

305 µl* PEQL45-2010-10D

1.5 mm 15 teeth

185 µl* PEQL45-2010-15D

1.5 mm 20 teeth

125 µl* PEQL45-2010-20D

1.5 mm 25 teeth

90 µl* PEQL45-2010-25D

0.8 mm 10 teeth

165 µl* PEQL45-2010-10C

0.8 mm 15 teeth

100 µl* PEQL45-2010-15C

0.8 mm 20 teeth

65 µl* PEQL45-2010-20C

0.8 mm 25 teeth

50 µl* PEQL45-2010-25C

Micro titer combs 1.5 mm 18 teeth

105 µl* PEQL45-2010-MTD

1.5 mm 36 teeth

50 µl* PEQL45-2010-MTC

0.8 mm 18 teeth

55 µl* PEQL45-2010-MT2D

0.8 mm 36 teeth

25 µl* PEQL45-2010-MT2C

Preparative combs 1.5 mm 2 teeth

3330/110 µl* PEQL45-2010-PD

0.8 mm 2 teeth

1776/59 µl* PEQL45-2010-PC

SpacerPlacer Facilitates the correct alignment of Spacers PEQL45-1010-SP

Spacer Sets

(2 side & 1 bottom spacer)

1.5 mm 1.2 cm wide

3 pieces PEQL45-2010-SD

0.8 mm 1.2 cm wide

3 pieces PEQL45-2010-SC

* volumes are calculated for a well length of 14 mm

Indice

Lingue:

Altri manuali VWR Apparecchiature di prova

VWR

VWR PH20 Manuale utente

VWR

VWR avantor SSTU Manuale utente

Manuali Apparecchiature di prova popolari di altre marche

SMART

SMART KANAAD SBT XTREME 3G Series Manuale utente

Agilent Technologies

Agilent Technologies BERT Serial Manuale utente

Agilent Technologies

Agilent Technologies N3280A Manuale utente

Vernier

Vernier Go Direct Voltage Manuale utente

Lifeloc

Lifeloc R.A.D.A.R. Manuale utente

Fluke

Fluke T5-600 Istruzioni per l'uso e l'installazione

Mercury

Mercury TSL01 Manuale utente

MAHA

MAHA MBT Series Manuale utente

Fuji Electric

Fuji Electric P-61 Manuale utente

ETC

ETC SealerAtor Manuale utente

Skyfine

Skyfine ETHYWAY V2 Manuale utente

Appion

Appion G5 Twin Manuale utente

PCB Piezotronics

PCB Piezotronics 8159-0112A Manuale utente

BW Technologies

BW Technologies MicroDock II Manuale utente

Sun Nuclear

Sun Nuclear 1027 Manuale utente

Biomark

Biomark HPR LITE READER Manuale utente

Hioki

Hioki 3169-20 Manuale utente

Ashcroft

Ashcroft ATE-100 Manuale utente