Waters 2707 Manuale

Waters 2707

Autosampler

Operator’s Guide

71500168202 / Revision A

OR PARTS THEREOF MAY NOT BE REPRODUCED IN ANY FORM

WITHOUT THE WRITTEN PERMISSION OF THE PUBLISHER.

The information in this document is subject to change without notice and

should not be construed as a commitment by Waters Corporation. Waters

Corporation assumes no responsibility for any errors that may appear in this

document. This document is believed to be complete and accurate at the time

of publication. In no event shall Waters Corporation be liable for incidental or

consequential damages in connection with, or arising from, its use.

Trademarks

Waters is a registered trademark and Empower and THE SCIENCE OF

WHAT’S POSSIBLE are trademarks of Waters Corporation.

Windows is a registered trademark of Microsoft Corporation.

PEEK is a trademark of Victrex®Corporation.

Teflon, Viton, and Tefzel are registered trademarks of E.I. duPont de

Nemours and Company.

Silco is a registered trademark of Silco Incorporated.

Phillips is a registered trademark of the Phillips Screw Company.

Valco is a registered trademark of Valco Instruments Inc.

Other registered trademarks or trademarks are the sole property of their

owners.

iii

Customer comments

Waters’ Technical Communications department invites you to tell us of any

errors you encounter in this document or to suggest ideas for otherwise

improving it. Please help us better understand what you expect from our

documentation so that we can continuously improve its accuracy and

usability.

We seriously consider every customer comment we receive. You can reach us

at tech_comm@waters.com.

Contacting Waters

Contact Waters®with enhancement requests or technical questions regarding

the use, transportation, removal, or disposal of any Waters product. You can

reach us via the Internet, telephone, or conventional mail.

Safety considerations

Some reagents and samples used with Waters instruments and devices can

pose chemical, biological, and radiological hazards. You must know the

potentially hazardous effects of all substances you work with. Always follow

Good Laboratory Practice, and consult your organization’s safety

representative for guidance.

When you develop methods, follow the “Protocol for the Adoption of Analytical

Methods in the Clinical Chemistry Laboratory,” American Journal of Medical

Technology, 44, 1, pages 30–37 (1978). This protocol addresses good operating

procedures and the techniques necessary to validate system and method

performance.

Decontamination information

For an explanation about how to decontaminate Waters liquid

chromatography/mass spectrometry (LC/MS) products, see the Waters

publication Controlling Contamination in LC/MS Systems (part number

715001307).

Waters contact information

Contacting medium Information

Internet The Waters Web site includes phone numbers

for Waters locations worldwide. Go to

www.waters.com, and click About Waters >

Worldwide Offices.

Telephone In the USA or Canada, phone 508-478-2000.

Conventional mail Waters Corporation

34 Maple Street

Milford, MA 01757

USA

Safety advisories

Consult Appendix A for a comprehensive list of warning and caution

advisories.

Operating this instrument

When operating this instrument, follow standard quality control procedures

and the guidelines in this section.

Intended use

Waters designed the 2707 Autosampler as a sample handler with a reliable,

precise fluid path versatile enough foruse in varied methods and applications.

The optional heating/cooling module accommodates a wide range of samples,

from heat labile biological samples to viscous polymer samples.

Quality control

Routinely run three quality-control samples that represent subnormal,

normal, and above-normal levels of a compound. Ensure that quality-control

sample results fall within an acceptable range, and evaluate precision from

day to day and run to run. Data collected when quality control samples are out

of range can be invalid. Do not report these data until you are certain that the

instrument performs satisfactorily.

Table of Contents vii

1 Understanding Operating Principles ................................................ 1-1

Describing the autosampler ........................................................................... 1-2

Listing component parts.................................................................................. 1-3

Identifying options ........................................................................................... 1-5

Exploring injection principles ....................................................................... 1-6

Defining pressure-assisted sample aspiration............................................... 1-6

Understanding syringe and buffer tubing...................................................... 1-7

Full loop injections........................................................................................... 1-8

Partial loop needle overfill injections ........................................................... 1-13

Partial loop injections.................................................................................... 1-18

Using well plates and vial trays .................................................................. 1-24

2 Preparing the Autosampler ................................................................. 2-1

Preparing for installation ............................................................................... 2-2

Setting up the instrument ............................................................................... 2-2

Plumbing the instrument ................................................................................ 2-3

Identifying instrument tubing ........................................................................ 2-7

Routing and connecting the tubing................................................................. 2-8

Working with waste tubing........................................................................... 2-10

Accommodating the wash solvent and syringe rinse................................... 2-10

Making Ethernet connections ...................................................................... 2-11

Controlling I/O connections ......................................................................... 2-12

Defining TTL inputs...................................................................................... 2-12

Explaining contact closure output ................................................................ 2-12

Table of Contents

viii Table of Contents

Connecting to the electricity source .......................................................... 2-13

Installing the prep kit .................................................................................... 2-14

Installing the biocompatibility kit .............................................................. 2-14

3 Maintaining the Autosampler ............................................................. 3-1

Outlining maintenance basics ....................................................................... 3-2

Contacting Waters Technical Service .......................................................... 3-2

Heeding maintenance considerations ......................................................... 3-3

Safety and handling......................................................................................... 3-3

Proper operating procedures........................................................................... 3-4

Cleaning the autosampler ............................................................................... 3-4

Removing or replacing the injection valve rotor seal .............................. 3-5

Installing a sample loop .................................................................................. 3-6

Replacing the sample needle .......................................................................... 3-6

Adjusting needle position ............................................................................... 3-8

Maintaining air needles .................................................................................. 3-8

Replacing air needles..................................................................................... 3-12

Table of Contents ix

Replacing a syringe ........................................................................................ 3-14

Replacing syringe plungers and plunger tips .......................................... 3-15

Replacing the syringe dispenser valve ...................................................... 3-16

Replacing fuses ................................................................................................ 3-19

4 Troubleshooting ..................................................................................... 4-1

Addressing instrument errors ....................................................................... 4-2

Running diagnostic tests ................................................................................. 4-2

Displaying the statuses of signal connections ........................................... 4-2

Changing the rear-panel interface connections ........................................ 4-3

Understanding power surges ......................................................................... 4-4

Understanding the status LED....................................................................... 4-4

Conducting analytical troubleshooting ....................................................... 4-4

A Safety Advisories .................................................................................. A-1

Warning symbols ............................................................................................... A-2

Task-specific hazard warnings........................................................................ A-2

Warnings that apply to particular instruments, instrument components, and

sample types A-3

Caution symbol .................................................................................................. A-5

Warnings that apply to all Waters instruments ......................................... A-5

Electrical and handling symbols ................................................................. A-13

Electrical symbols.......................................................................................... A-13

Handling symbols .......................................................................................... A-14

x Table of Contents

B Materials of Construction and Compliant Solvents ..................... B-1

Preventing contamination ............................................................................. B-2

Naming solvents for mobile phase preparation ....................................... B-2

C Specifications ........................................................................................ C-1

Outlining general specifications .................................................................. C-2

Outlining prep version specifications ........................................................ C-6

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