Waters Extraction+ Manuale utente

USER MANUAL

Extraction+

Safety instructions

• Do not use this device for:

- evacuating biological habitats

- evacuating explosive, corrosive, or similar gases

• The device is not suitable for:

- inating/deating objects

- pumping liquids

- use in potentially explosive atmospheres

- underwater usage

- underground usage

• Set up the Extraction+ vacuum pump in a spacious area on an even,

stable, clean, nonslip, dry surface.

• Completely switch o the device and remove cables before

moving it.

• The user must maintain a distance of at least 20 cm from the

product (WiFi antenna) during installation or operation.

• The device is intended for indoor use only.

• With the exception of lter cartridge replacement, device

maintenance is exclusively reserved for Waters servicing and support

representatives.

• If the device is used in any way other than those specied by the

manufacturer, the user protection provided by the device may be

impaired.

• The safety of the user cannot be ensured if modications are made

to the device or parts of the device by third parties.

• All input/output ports are Safety Extra Low Voltage (SELV) circuits.

You should only connect SELV circuits to other SELV circuits.

• If the device overheats, it will stop operating. If this occurs,

immediately disconnect the device from its power supply.

About this User Manual

Read the User Manual before using this device. Strictly follow usage and maintenance

instructions provided. It is the user’s responsibility to become familiar with all available

information concerning the correct use, care, and limitations of these products. If you are

uncertain about the correct use or limitations of the products, contact Waters service and

support. The manufacturer, the distributor, and their respective owners, employees, agents, and

representatives are not responsible or liable for errors or omissions.

Information in this User Manual is subject to change without notice and does not represent a

commitment on the part of Waters. Waters assumes no responsibility for any inaccuracies that

may be contained in this User Manual. Waters makes no commitment to update or keep current

the information in this User Manual, and reserves the right to make improvements to this User

Manual or to the products described herein at any time without notice.

If you nd information in this manual that is incorrect, misleading, or incomplete, we would

appreciate your comments and suggestions.

Product overview & setup

Extraction+ vacuum pump

Extraction+ manifold

Built-in wireless

adapter

Built-in

computer

LED indicator Vacuum inlet Power button

Rear view

Ethernet for

cable

connection to

network

Maintenance hatch

Waste container

(can substitute any

GL45-threaded

bottle)

Silicone

vacuum

hose

Collar lifter

Extraction labware

(column in adapter or

cartridge)

Collection labware

(tubes in adapter

or plate with manifold spacers)

Manifold base, waste

collector

Manifold collar

Power socket

Getting started

A. Install the vacuum pump and liquid trap system

Connect the Extraction+ vacuum pump

and the waste container using the silicone hose

If needed, cut the silicone hoses to the appropriate lengths

Bottle smaller than one

liter can also be placed

on the Extraction+

vacuum pump.

On or under the workbench,

or on the oor.

On or under the workbench,

or on the oor.

We recommend using the longest portion of

the hose for connecting the Extraction+ vacuum

pump to the waste container.

Place the Extraction+ vacuum

pump

Place the waste container

(GL45-threaded bottle)

1 2

Ensure that the connection is loose

enough to prevent the waste container

from falling.

This hose must be connected to the

shorter port of the thread.

B. Installation of the manifold

Setup for cartridge

When used with the Andrew+ Pipetting Robot,

the Extraction+ vacuum manifold is always

placed at the same deck location:

Domino location 5

Setup for lter plate

Plug the silicone hose between

the waste container and the

Extraction+ manifold

Plug the silicone hose between

the waste container and the

Extraction+ manifold

Place the manifold spacers into the waste

collector (see additional instructions on

the next page)

Place the manifold collar lifter

Place the manifold collar

Place the cartridge adapter as OneLab

prompts you to

Place the lter plate as OneLab prompts

you to

This hose must be connected to

the elbow port of the thread.

This hose must be connected to

the elbow port of the thread.

B.1. Manifold spacers (only for use with lter plate)

The plate spacers allow ne-tuning of the

penetration height of the lter plate tips within the

wells of the collection labware. Ideal penetration

is 2-3 mm.

The spacer system is composed of three sizes:

The spacers can be

placed into the manifold

waste collector...

...and can be combined to

further prop up collection

plates if necessary

(example here:

3 mm spacer

+ 15 mm spacer)

Examples of optimal spacer usage

for dierent lter and collection consumable combinations

3 mm 15 mm 17 mm

Extraction plate

skirt length

Collection

consumable

Size 1:

Small skirt

Size 2:

Long skirt

Waters 2-mL 96-square well

collection plate, cut corner H1

1 × 3 mm spacer 1 × 17 mm spacer

on top of 1 × 3 mm spacer

Waters 0.7-mL 96-round well

collection plate

1 × 15 mm spacer 1 × 17 mm spacer

on top of 1 × 15 mm spacer

Waters QuanRecovery™ 700-µL

96-well plate

1 × 15 mm spacer 1 × 17 mm spacer

on top of 1 × 15 mm spacer

Waters 0.8-mL 96-round well

collection plate

1 × 15 mm spacer 1 × 17 mm spacer

on top of 1 × 15 mm spacer

Extraction labware tip

Collection labware

Tip penetration

2–3 mm

Small skirt

Long skirt

B.2. Use of plate sealer (only for lter plate use)

To apply vacuum evenly on each lter plate

cavity, we recommend placing a plate-sealer

lm on top of unused lter plate cavities.

To apply vacuum evenly on each cartridge,

we recommend placing sealing caps in

each of the unused slots of the cartridge

adapter.

When using cartridges as

lter consumables during

an automated run with the

Andrew+ robot, OneLab

prompts the usage of a

Collection Labware Rack Domino

in addition to the collection

rack itself.

Ordering info: Adhesive Seal for 96-well

and 384-well Plates, 100/pk, Waters p/n:

186006336C

Contact your Waters representative.

Ordering info: Microplate Gripper (Tool),

Waters p/n: 186009776

Contact your Waters representative.

This Domino holds the collection consumables

during the rst phases of the solid-phase

extraction, such as: conditioning, loading, and

washing.

The Collection Labware Rack Domino is included

in each Extraction+ Cartridge Kit. You can also

purchase it separately.

Sample available in the packaging

B.4. Collection labware rack (only for cartridge use)

B.3. Use of sealing caps (only for cartridge use)

B.5. Labware gripper (only for Andrew+ use)

Cut o the desired dimensions and

apply the lm on the plate over the

unused cavities

Add the Microplate Gripper

tool on the Andrew+

robot tool rack to ensure

automated movement of

collection labware and racks

into and out of the manifold.

C. Connection to OneLab

If you have a OneLab account, plug

your Extraction+ vacuum pump

into a power source.

Wait for the LED indicator to

turn blue and blink.

After a couple of minutes,

the Extraction+ vacuum

pump will inform you

when it is in installation

mode.

Connect your computer, tablet, or smartphone to the Extraction+ vacuum pump.

Launch a Web browser, type http://192.168.2.1,and follow the on-

screen steps to continue installation.

A. Go to your WiFi settings

and select the network named Extraction-xxx.xxxx.xxxxx.

1 2

scan me

Note that the installa-

tion page might open

automatically.

If you do not have access to a

WiFi-compatible device, refer to

our online Help:

help.andrewalliance.com

xxxx.xxxxx is the Extraction+ device serial number (found on the back of the

Extraction+ vacuum pump or on top of the Extraction+ vacuum manifold).

B. The LED indicator stops blinking when the device is successfully

connected.

Extraction-xxx.xxxx.xxxxx

If the Extraction+ vacuum pump does not

automatically enter installation mode,

press the power button ve times.

http://192.168.2.1

Before

you begin

If you do not have a OneLab account, create one at:

https://onelab.com/signup

The device will automatically start up.

Only use the provided AC/DC

power supply unit and cords.

Operations

Design your protocol

Execute experiment with

Extraction+

Start the experiment and let OneLab

control your device

OneLab automatically selects your

Extraction+ and allows you to use it with each

available experiment setup, manual, semi-

automated, or fully automated operation.

Choose your desired experiment setup, and

then prepare all the required materials.

Our drag-n-drop editor makes it easy to

design a precise and complete protocol with all

you need to run your

experiment perfectly.

Create a Flow-through extraction

action by clicking this menu, which

is located on the left side of the

screen.

Actions

Follow the visual instructions based on the

protocol you are using and you will be guided

through your experiment.

Each step of the experiment is recorded and

available in OneLab.

Feel free to contact

the Waters

support team Send us an email at

[email protected]

Chat with our support team

directly in OneLab

We reply within one business day

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