X-Cite Exacte Manuale utente

X-Cite®exacte User Guide

X-Cite® exacte User Guide

035-00283 Rev. 3

Made in Canada

Excelitas Canada Inc. 2016

No part of this publication may be reproduced, transmitted, transcribed, stored in a

retrieval system or translated into any language in any form by any means without the

prior written consent of Excelitas Canada Inc. Every effort has been made to ensure

information in this manual is accurate; however, information in this manual is subject to

change without notice and does not represent a commitment on the part of the authors.

Excelitas Canada Inc.

2260 Argentia Road

Mississauga ON L5N 6H7 CANADA

Trademarks

X-Cite®, exacte, Intelli-Lamp®, and Closed-Loop Feedback are registered trademarks of

All other product names are trademarks of their respective owners.

Table of Contents

1. X-Cite®exacte Message Reference.......................................................................1

2. Introduction............................................................................................................2

3. Safety......................................................................................................................3

3.1 Glossary of Symbols...........................................................................................3

3.2 Safety Precautions .............................................................................................4

4. Getting Started.......................................................................................................7

4.1 System Components..........................................................................................7

4.2 Front Panel.........................................................................................................8

4.3 Rear Panel.........................................................................................................9

5. Installation/Set-up................................................................................................10

5.1 Installing Lamp Module.....................................................................................10

5.2 Inserting/Removing Light Guide .......................................................................13

5.3 Tips to Prevent Premature Degradation of Light Guides...................................15

5.4 Installing the Collimating Adapter .....................................................................16

6. Operation..............................................................................................................18

6.1 Powering Up/Powering Down...........................................................................18

6.2 Quick Start: Illuminating a Specimen................................................................19

6.3 Front Panel Button Functions...........................................................................19

6.4 LED Indicators..................................................................................................20

6.5 Display Format Reference................................................................................20

6.6 Output Intensity Modes.....................................................................................21

6.7 Adjusting Light Output......................................................................................21

6.8 Viewing Accumulated Lamp Hours...................................................................22

6.9 Closed-Loop Feedback™ (CLF).......................................................................22

7. Calibration/Radiometry (optional).......................................................................24

7.1 X-Cite®exacte Power Reading using the XR2100 Power Meter or XR2000;....24

7.2 X-Cite®exacte Calibration using the XR2100 (or XR2000) & Light Guide.........25

7.3 Calibrating X-Cite®exacte with the Objective Plane Power Sensor, XP750......25

7.4 To Clear a Calibration.......................................................................................26

8. Warnings and Alarms..........................................................................................27

8.1 Clearing Alarms................................................................................................28

9. External Control...................................................................................................29

9.1 Graphical User Interface (GUI).........................................................................29

9.2 Installation:.......................................................................................................29

9.3 Graphical User Interface Panel.........................................................................30

9.4 Icons and Functions: ........................................................................................31

9.5 Logging Stability Data.......................................................................................33

9.6 Logging Events.................................................................................................33

9.7 Exporting Logged Data.....................................................................................33

9.8 Shutter Control via Foot pedal..........................................................................34

9.9 Shutter control via TTL.....................................................................................34

9.10 Software Developer’s Kit (SDK) .......................................................................35

10. Troubleshooting...................................................................................................36

10.1 Error Codes...................................................................................................36

10.2 Power Up Failure ..........................................................................................37

10.3 Lamp Strike Failure.......................................................................................37

10.4 Low Light Intensity ........................................................................................38

10.5 Shutter Failure...............................................................................................38

10.6 LED Display Failure ......................................................................................38

10.7 Cooling Fan Failure.......................................................................................38

11. Care and Maintenance.........................................................................................39

11.1 General.........................................................................................................39

11.2 Liquid Light Guides .......................................................................................40

11.3 Lamps...........................................................................................................43

11.4 Replacing the Lamp Module..........................................................................44

11.5 Air Vents & Filters .........................................................................................45

11.6 Replacing the Air Filter..................................................................................47

11.7 Replacing the External Fuses........................................................................47

12. Technical Specifications .....................................................................................48

12.1 Electrical .......................................................................................................48

12.2 Environmental...............................................................................................48

12.3 Lamp.............................................................................................................49

12.4 Light Guide....................................................................................................50

12.5 General.........................................................................................................50

13. Regulatory............................................................................................................51

13.1 Product Safety and Electromagnetic Compatibility: .......................................51

13.2 China RoHS..................................................................................................52

14. Warranty...............................................................................................................53

15. Contact Information.............................................................................................54

15.1 Replacement Parts and Accessories.............................................................54

X-Cite®exacte User Guide

035-00283 rev 3

1. X-Cite®exacte Message Reference

Display

Message

Definition

Description

XXX

Intensity Setting (%)

Displays the iris setting, which gives relative intensity as

percentage of the lamp’s maximum output

XX.XX

Power Setting (watts)

Displays the power setting, which gives absolute

intensity in terms of watts

uXXX

Power Setting (microwatts)

Displays the power setting, which gives absolute

intensity in terms of microwatts

X.XXX

XXX.X

Power Setting (milliwatts)

Displays the power setting, which gives absolute

intensity in terms of milliwatts

XXXX.

Lamp Hours

Displays the “hours of use” accumulated by the lamp

Flashing:

“XXXX”

Warming Up

The lamp is warming up. Warm up time is

approximately 4 minutes ref section 6.1

bulb

Lamp Error

Lamp installed incorrectly /

Lamp did not strike. See section 10.3

cool

Lamp is too hot to strike

The lamp will automatically strike when it has cooled to

the optimum striking temperature. See section 10.3

Alternating:

old / bulb

Old Lamp

The lamp has accumulated over 2500 hours. Lamp may

be near end of life.

Alternating:

end / bulb

New Lamp Required

The lamp has reached end of life. The lamp will not

strike.

LOC

Front Panel Locked

All front panel buttons have been locked to prevent

settings from being changed.

SFI

Shutter Failure

The shutter has failed to return to home position. Unit

should be restarted. If the error repeats, contact Tech

Support.

Lamp temperature is too high

Check ventilation filters and outlets to ensure that no

blockage exists. Restart unit and if problem persists

replace lamp. If problem continues contact Tech

Support

Internal communication failure

Restart unit, if problem persists contact Tech Support

Iris failed to go to home position

Restart unit, if problem persists contact Tech Support

Internal hardware failure

Restart unit, if problem persists contact Tech Support

Failure to communicate with Intelli-

Lamp®

Check Intelli-Lamp®connection and restart unit. If

problem persists try another lamp, if problem still persist

contact Tech Support.

Lamp self extinguished

Let system cool and restart lamp. If it goes out again,

replace lamp. If new lamp continues to self-extinguish,

contact Tech Support

Internal system error

Restart unit, if problem persists contact Tech Support

See Section 10 for troubleshooting.

X-Cite®exacte User Guide

035-00283 rev 3

2. Introduction

The X-Cite®exacte is a revolution in fluorescence microscopy illumination. Like the

previous X-Cite®illuminators, the X-Cite®exacte has the convenience of pre-aligned,

long-life Intelli-Lamps®, easy installation and compatibility will all major microscope

brands. With additional features such as Closed-Loop Feedback™, intensity adjustment

in 1% increments, a high speed internal shutter, and a calibrated output option which is

compatible with the X-Cite®Optical Power Measurement System, the X-Cite®exacte

reaches a new level of performance that makes it the ideal choice for quantitative,

comparative, and live cell imaging.

Excelitas Technologies Corp. is a global technology leader focused on delivering

innovative, customized solutions to meet the lighting, detection and other high-

performance technology needs of customers. X-Cite®, formerly of Lumen Dynamics

(which was acquired by Excelitas Technologies Corp. in November 2013) offers the Life

Science and Analytical Instrumentation market a broad range of innovative lamp and

LED fluorescence illumination and measurement solutions.

The X-Cite®exacte is a high quality product manufactured in accordance with ISO 9001,

CE marked and certified to UL, China RoHS and CSA standards.

We suggest that you read this manual to discover all its features, and how to use them.

Thank you for choosing X-Cite®!

X-Cite®exacte User Guide

035-00283 rev 3

3. Safety

3.1 Glossary of Symbols

CAUTION - Risk of danger: consult accompanying documents

WARNING -Eye damage may result from directly viewing ultraviolet light –

protective eye shielding and clothing must be used at all times.

Input/Output Signals

Input Signal

CAUTION –Hot Surface

X-Cite®exacte User Guide

035-00283 rev 3

3.2 Safety Precautions

The X-Cite®exacte is equipped with two safety sensors to protect the user from

accidental UV exposure. In addition, please observe the following precautions during

use. This series of cautions, warnings and dangers relate to the operation and

maintenance of the X-Cite®exacte. They are also presented throughout this User’s

Guide where necessary.

Warning

Eye damage may result from directly viewing the light produced by the lamp used

in this product. Always use protective eyewear and turn the lamp off before

removing cover.

Caution

Never look into the light emitting end of the light guide. The light could severely

damage the cornea and retina of the eye if the light is observed directly. Eye

shielding must be used at all times as well as clothing to protect exposed skin.

Warning

Always make sure the light guide is properly inserted into the X-Cite®exacte and

the microscope prior to turning on power to the unit. This will minimize the risk of

exposure to the UV light.

Caution!

To prevent damage/ degradation of the light guide, always allow adequate

clearance at the front of the X-Cite®exacte unit to prevent kinking or excessive

bending.

Warning

To reduce the risk of fire or shock, always replace the fuses with the same type

and rating.

Warning

Disconnecting of main supply source is only possible by unplugging the power

cord.

Danger

This unit contains HIGH VOLTAGE components. It is recommended that ONLY

QUALIFIED TECHNICAL PERSONNEL perform any testing or repairs described in

this manual. Disconnect the AC power cord from the unit before opening the cover

of this unit. All cover screws must be replaced prior to applying power to the unit, or

safety of the unit will be impaired.

X-Cite®exacte User Guide

035-00283 rev 3

Monitoring the unit during manual operation

The level of UV and visible energy supplied by the X-Cite®exacte is sufficient to

ignite flammable substances. During manual operation, the unit must be attended

at all times by a qualified operator. The unit must not be left unattended while

turned on. If an operator leaves the work area of the unit, the lamp power switch

must be turned off.

Monitoring the unit during Automated operation

The level of UV and visible energy supplied by the X-Cite®exacte is sufficient to

ignite flammable substances. Therefore, when the unit is operated unattended in

an automated environment, an alarm function must be provided by the user to

indicate a malfunction in the associated equipment used.

Warning

Hg –LAMP CONTAINS MERCURY, Manage in Accord with Disposal Laws,

see: www.lamprecycle.org or 1-800-668-8752

Danger: Exposure to Mercury represents a health hazard to humans.

When unpacking or installing the lamp, always wear protective clothing and a face

mask. Operate the lamp inside the X-Cite®exacte housing only. This prevents

direct viewing of the arc and in the case of lamp bursting, contains the lamp

particles. In the rare instance in which a lamp bursting occurs, and the mercury

content is released, the following safety precautions are recommended; all

personnel should be immediately evacuated from the area to prevent inhalation of

the mercury vapour. The area should be well ventilated for a minimum of 30

minutes. Prior to clean up ensure an ‘approved-for-mercury’ respirator mask and

non-porous gloves such as latex or rubber are used. After the lamp housing

elements have cooled, the mercury residue should be collected with the use of a

special absorbing agent available from laboratory equipment suppliers.

Listed below are examples of internet web sites for obtaining Mercury Spill Kits;

http://www.rosshealthcare.org/Mercon.spill.kits.htm

http://www.coleparmer.ca/catalog/product_index.asp?cls=43577

http://www.environmental-

expert.com/technology.aspx?idCategory=2054&word=mercury%20spill%20kits

http://www.alibaba.com/products/spill_kit/4.html

Warning

Should this X-Cite®exacte unit be used in a manner not specified by Excelitas

Technologies, the protection provided by the equipment may be impaired.

X-Cite®exacte User Guide

035-00283 rev 3

Warning

The method in which lamps are disposed of must comply with local rules and

regulations for disposal of hazardous materials. Lamps may be returned to

Excelitas Technologies providing they are returned in their original packaging.

Excelitas Technologies will dispose of them in the appropriate manner.

Warning

This unit is designed for bench top use only! Always ensure that the unit is

operated on a hard, stable surface. This will prevent obstruction of the bottom

chassis ventilation openings. Any obstruction of these openings could result in a

possible over-heating condition. Do not attempt to remove or tamper with the

rubber feet located on the bottom of the unit.

Caution

The lamp module’s operational life can be significantly shortened if it is handled

incorrectly. Do not touch the bulb’s glass envelope or the inside surface of the

reflector. Skin oils can cause the lamp module to fail prematurely.

Caution

Prior to opening the unit and handling the lamp module, allow a minimum of 5

minutes for the lamp module to cool down completely.

Caution

Any electronic equipment connected to the X-Cite®exacte must comply with the

requirements of EN/IEC 60950.

Cleaning:

Clean the exterior of the unit with a slightly dampened cloth and simple water/

detergent solution only.

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