YSI 2700 SELECT Manuale utente

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Biochemistry Analyzer



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1.1 Description 1-1

1.2 Standard Features 1-3

1.3 General Specifications 1-4

1.4 Chemistry Performance Specifications 1-5

1.5 How to Use this Manual 1-8

  

2.1 Unpacking and Notes on Safety 2-1

2.2 Major Component Identification 2-2

2.3 Reagent Preparation 2-5

2.4 Enzyme Membrane Installation 2-6

2.5 Power Up Procedures 2-8

2.6 Fluid System Priming 2-9

2.7 Printer Paper Installation 2-11

2.8 Instrument Parameter Programming 2-12

2.9 Initial Setup Example: Step-by-Step 2-15

2.10 Probe Baseline Check 2-25

  

3.1 Main Menu 3-1

3.2 Run Mode 3-2

3.3 Standby Mode 3-7

3.4 Daily Operational Checks 3-7

  

4.1 Principle 4-1

4.2 Sample Preparation 4-2

4.3 Measurement Parameter Setup Information 4-3

4.4 D-Glucose (Dextrose) 4-5

4.5 L-Lactate 4-6

4.6 Sucrose 4-7

4.7 Lactose 4-8

4.8 Ethanol 4-9

4.9 Starch 4-10

4.10 Choline 4-11

4.11 Galactose 4-12

4.12 Hydrogen Peroxide 4-13

4.13 L-Glutamate (L-Glutamic Acid) 4-14

4.14 L-Glutamine 4-15

4.15 Methanol 4-16

4.16 Simultaneous Dextrose and L-Lactate 4-17

4.17 Simultaneous Dextrose and Sucrose 4-18

4.18 Simultaneous L-Glutamate and L-Glutamine 4-20

  

5.1 Introduction 5-1

5.2 Service Selections 5-3

5.3 Setup Selections 5-5

5.4 Diagnostic Selections 5-17

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6.1 Sensor Technology 6-1

6.2 Fluid System 6-3

6.3 Measurement Methodology 6-4

6.4 Baseline Stability 6-4

6.5 Calibration 6-5

6.6 Linearity 6-5

6.7 Temperature Compensation 6-6

6.8 Level Sensing and Sipper Interference 6-6

6.9 Software Structure 6-7

! 

7.1 Daily Maintenance 7-1

7.2 Calibration Pumping System Maintenance 7-3

7.3 Sample Chamber Cleaning 7-4

7.4 Membrane Replacement 7-5

7.5 Probe Cleaning 7-6

7.6 Tubing Replacement 7-7

7.7 Sipper Replacement 7-9

7.8 Sipper Ground Cable 7-9

7.9 Fuse Replacement 7-10

7.10 Sipper Pump Seal Replacement 7-11

7.11 Sipper Mechanism Lubrication 7-13

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8.1 Printout Information 8-2

8.2 Troubleshooting Chart 8-4

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9.1 Communications Protocol 9-1

9.2 Communications Modes 9-5

9.3 Communications Commands 9-6

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I-1

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Figure 2.1 Inside Front View of the 2700 SELECT 2-2

Figure 2.2 Back View of the 2700 SELECT 2-4

Figure 2.3 Bottles and Level Sensor Cables 2-5

Figure 2.4 Sample Chamber/Sensors 2-6

Figure 2.5 Enzyme Membrane Installation 2-7

Figure 2.6 Line Voltage Selector 2-8

Figure 2.7 Sipper Adjustment Position 2-10

Figure 3.1 2700 SELECT Software Structure 3-1

Figure 3.2 Sampling Stations 3-4

Figure 3.3 The Test Tube Holder Pivoted Out 3-4

Figure 3.4 The Manual Station 3-6

Figure 5.1 2700 SELECT Software Structure 5-1

Figure 5.2 YSI 2700 SELECT Menu Flow Chart 5-2

Figure 5.3 2700 SELECT Major Components 5-17

Figure 6.1 Sensor Probe and Enzyme Membrane 6-1

Figure 6.2 2700 SELECT Fluid System 6-3

Figure 6.3 Typical Sensor Response 6-4

Figure 6.4 Aging Membrane Response 6-5

Figure 6.5 2700 SELECT Software Structure 6-7

Figure 7.1 Sample Chamber Illustration 7-5

Figure 7.2 Tubing Connections 7-7

Figure 7.3 Pump Tubing installation 7-8

Figure 7.4 Pump Assembly 7-9

Figure 7.5 Fuse Replacement 7-10

Figure 7.6 Sipper Pump Head Removal 7-11

Figure 7.7 Sipper Pump Seal Replacement 7-12

Figure 7.8 Sipper Pump Plunger Position 7-12

Figure 7.9 Sipper Mechanism Drive Screw and Guide Rods 7-13

Figure 9.1 RS-232 Signal Description and Direction 9-1

Figure 9.2 Full Handshaking DTE Interface 9-2

Figure 9.3 Three Wire DTE Interface 9-2

Figure 9.4 2700 SELECT Data Report Format 9-7

Figure 9.5 Report Format Field Information 9-8

Figure 9.6 RM Command Report Format 9-15

Figure 9.7 RI Command Report Format 9-16

Figure G.1 Line Power Cord and Plug Wiring G-1

1-1

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 

TheYSI Model 2700 SELECT Biochemistry Analyzer is a laboratory instrument intended for

use in research, food-processing and bioprocessing applications. THE MODEL 2700 SELECT

IS NOT FOR HUMAN MEDICAL DIAGNOSTIC USE OR FOR HUMAN

PERFORMANCE EVALUATION.

The Model 2700 SELECT offers precision and specificity comparable to more time consuming

and rigorous methods, but with greater speed, convenience and sensitivity. Many of the

conditions which interfere with refractometers, density meters and manual methods are of little

concern when using the Model 2700 SELECT. Measurements are virtually unaffected by

sample color, turbidity, density, viscosity, pH, volatility, specific gravity, temperature, index of

refraction, optical activity, or the presence of proteins or other biochemicals.

The Model 2700 SELECT can be set up to measure a number of different analytes, but

switching between them may entail some time and effort. Configured as a single channel unit,

the Model 2700 SELECT provides quick measurements of any one of the following analytes:

•D-Glucose (Dextrose)

•L-Lactate

•Sucrose

•Lactose

•Ethanol

•L-Glutamate

•Choline

•L-Glutamine

•Methanol

•Galactose*

•Hydrogen Peroxide*

Configured as a dual channel unit, the Model 2700 SELECT provides quick, simultaneous

measurements of combinations of the following analytes:

•Dextrose and L-Lactate

•Dextrose and Sucrose

•L-Glutamate and L-Glutamine

*YSI does not currently offer calibration standards for these analytes.

1-2

Flexibility is an important feature of the product. Within practical limits a large number of

measurement parameters can be selected to fit your application. In addition, the default

parameters of many system functions can be changed to fit your needs.

Selectable sample size allows you to extend the dynamic range of the analyzer and improves

precision and accuracy. The aspirated sample size can be specified from a minimum of 5

microliters to a maximum of 65 microliters.

The Model 2700 SELECT is equipped with automatic self-calibration. However, within limits,

the frequency of autocalibration can be programmed to meet your needs.

If instrument calibration is desired upon command only, the self-calibration feature can be

disabled and calibration cycles can be initiated manually via the keypad or commanded by the

host computer via the RS-232 interface.

YSI provides a full line of reagents to support the most common chemistries.

YSI offers the Model 2710 Turntable for batch sampling of up to 24 tubes or vials. The 2710

Turntable is controlled by the 2700 SELECT software via a control cable (provided). The

results of batch sampling are displayed and printed by the 2700 SELECT.

YSI offers the Model 2730 Monitoring and Control Accessory for use in applications where

monitoring and control is desired. The Kit contains a peristaltic pump, external sampling

chamber, and tubing for delivering a sample to the 2700 SELECT. Purge time and sampling

interval are selectable, under control of the 2700 SELECT software. Also included in this

accessory are analog outputs representative of analyte concentrations.

The 2700 SELECT has been designed for future additions to allow even greater versatility

related to sample handling and the measurement of additional analytes. Therefore, as

accessories become available or new chemistries are developed you have a basic instrument

upon which to build.

1-3

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•Single and dual chemistry configurations.

•Minimal sample preparation.

•Sample aspiration from a variety of tubes.

•Selectable sample aspiration volume (5 to 65 µL).

•Sample results in about one minute.

•Fluid sensing minimizes carry-over.

•Fluid design washes inside and outside of sipper tube.

•Programmable automatic calibration.

•Selectable concentration units, including g/L, mg/L, mmol/L, %(w/v).

•Liquid crystal display and built-in data printer.

•RS-232 serial port.

•Turntable interface circuitry installed.

•User-accessible service and diagnostic menus.

1-4

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Sample size: Adjustable from 5 to 65 microliters (aspirated volume)

Response Time: Sample results in 60 seconds,

Complete sample-to-sample cycle in 90 seconds

(May vary with analyte and sample matrix.)

Power requirement: 110-120 VAC or 220-240 VAC

50-60 Hz

50 Watts nominal

Working environment:

Ambient temperature: 15° to 35° Celsius

Relative humidity: 10% to 90% (non-condensing)

Instrument dimensions: 10.0 x 14.0 x 14.0 inches

25.4 x 35.6 x 35.6 centimeters

Instrument weight: 25 pounds

11.4 kilograms

1-5

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Dextrose (D-Glucose)

YSI Membrane: 2365

Detection Range: 0-9 g/L at 25 µL sample size,

0-25 g/L at 10 µL sample size

Calibration Point: 2.50 g/L

Linearity Check Point: 9.0 g/L

Precision (CV,n=10): 2%

Linearity (0 to Cal Point): ±2%

Linearity (Cal to Range Max): ±5%

Typical Membrane Working Life: 21 days

Membrane O-ring Color: Red

Ethanol

YSI Membrane: 2786

Detection Range: 0-3.2 g/L

Calibration Point: 2.00 g/L

Linearity Check Point: 3.20 g/L

Precision (CV,n=10): 2%

Linearity (0 to Cal Point): ±2%

Linearity (Cal to Range Max): ±5%

Typical Membrane Working Life: 5 days

Membrane O-ring Color: Green

Potential Substrate Interference: Methanol

L-Glutamate*

YSI Membrane: 2754

Detection Range: 0-10 mmol/L

Calibration Point: 5.00 mmol/L

Linearity Check Point: 10.0 mmol/L

Precision (CV,n=10): 2%

Linearity (0 to Cal Point): ±2%

Linearity (Cal to Range Max): ±5%

Typical Membrane Working Life: 7 days

Membrane O-ring Color: Yellow

*User may configure instrument to measure as monosodium glutamate (MSG).

L-Lactate (L-Lactic Acid)

YSI Membrane: 2329

Detection Range: 0-2.67 g/L

Calibration Point: 0.50 g/L

Linearity Check Point: 2.67 g/L

Precision (CV,n=10): 2%

Linearity (0 to Cal Point): ±2%

Linearity (Cal to Range Max): ±5%

Typical Membrane Working Life: 14 days

Membrane O-ring Color: Gray

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