Agilent Technologies 4150 TapeStation system Guida utente

Agilent Technologies

Agilent 4150 TapeStation

System

System Manual

Agilent 4150 TapeStation System Manual

Notices

No part of this manual may be reproduced

in any form or by any means (including

electronic storage and retrieval or transla-

tion into a foreign language) without prior

agreement and written consent from Agi-

lent Technologies, Inc. as governed by

United States and international copyright

laws.

Manual Part Number

G2992-90000

Edition

12/2018

Printed in Germany

Agilent Technologies

Hewlett-Packard-Strasse 8

76337 Waldbronn

Warranty

The material contained in this docu-

ment is provided “as is,” and is sub-

ject to being changed, without notice,

in future editions. Further, to the max-

imum extent permitted by applicable

law, Agilent disclaims all warranties,

either express or implied, with regard

to this manual and any information

contained herein, including but not

limited to the implied warranties of

merchantability and fitness for a par-

ticular purpose. Agilent shall not be

liable for errors or for incidental or

consequential damages in connection

with the furnishing, use, or perfor-

mance of this document or of any

information contained herein. Should

Agilent and the user have a separate

written agreement with warranty

terms covering the material in this

document that conflict with these

terms, the warranty terms in the sep-

arate agreement shall control.

Technology Licenses

The hardware and/or software described in

this document are furnished under a

license and may be used or copied only in

accordance with the terms of such license.

Restricted Rights Legend

If software is for use in the performance of

a U.S. Government prime contract or sub-

contract, Software is delivered and licensed

as “Commercial computer software” as

defined in DFAR 252.227-7014 (June 1995),

or as a “commercial item” as defined in

FAR 2.101(a) or as “Restricted computer

software” as defined in FAR 52.227-19

(June 1987) or any equivalent agency regu-

lation or contract clause. Use, duplication

or disclosure of Software is subject to Agi-

lent Technologies’ standard commercial

license terms, and non-DOD Departments

and Agencies of the U.S. Government will

receive no greater than Restricted Rights as

defined in FAR 52.227-19(c)(1-2) (June

1987). U.S. Government users will receive

no greater than Limited Rights as defined in

FAR 52.227-14 (June 1987) or DFAR

252.227-7015 (b)(2) (November 1995), as

applicable in any technical data.

Safety Notices

CAUTION

A CAUTION notice denotes a

hazard. It calls attention to an

operating procedure, practice, or

the like that, if not correctly per-

formed or adhered to, could

result in damage to the product

or loss of important data. Do not

proceed beyond a CAUTION

notice until the indicated condi-

tions are fully understood and

met.

WARNING

A WARNING notice denotes a

hazard. It calls attention to an

operating procedure, practice,

or the like that, if not correctly

performed or adhered to, could

result in personal injury or

death. Do not proceed beyond a

WARNING notice until the indi-

cated conditions are fully

understood and met.

Agilent 4150 TapeStation System Manual 3

In this Guide...

This manual covers the 4150 TapeStation system

1Safety

This chapter provides information on safety symbols and safety messages.

2 Legal and Regulatory

This chapter provides additional information on legal and regulatory aspects.

3 Product Description

This chapter gives an introduction to the instrument and provides information

on intended use and instrument specifications.

4 Installation

This chapter gives information about the installation of the system.

5TapeStationSoftware

This chapter gives an overview on the three packages within the TapeStation

software.

6 Operating Instructions

This chapter explains the operation of the 4150 TapeStation system.

7 Good Measurement Practices

This chapter summarizes information on good measurement practices for

ScreenTape assays.

8 Instrument Diagnosis

This chapter contains information on instrument diagnosis, available tests,

and reporting.

4 Agilent 4150 TapeStation System Manual

In this Guide...

9 Maintenance

This chapter describes the maintenance of the 4150 TapeStation system.

10 Troubleshooting

This chapter gives an overview about the troubleshooting features of the 4150

TapeStation system. It includes information about application troubleshooting

as well.

11 Appendix

This chapter contains parts information.

Agilent 4150 TapeStation System Manual 5

Contents

1Safety 9

Symbols 10

Safety Messages 12

General Safety Information 13

2 Legal and Regulatory 15

Sound Emission 16

Waste Electrical and Electronic Equipment (WEEE) Directive 17

3 Product Description 19

Intended use of the 4150 TapeStation System 20

Overview of the System and ScreenTape Architecture 21

Product Descriptions 25

Physical Specification 32

4 Installation 33

Site Requirements 34

Bench Space Required 36

Unpacking 38

Initial Setup of the System 45

Prepare the Working Area 48

5 TapeStation Software 51

TapeStation Controller Software 52

TapeStation Analysis Software 58

Agilent Information Center 60

6 Agilent 4150 TapeStation System Manual

Contents

6 Operating Instructions 63

Sample Preparation 64

Preparing the Instrument 65

Starting the Analysis 73

Analysis End 77

Follow Up 78

7 Good Measurement Practices 81

Use of Correct Tools 83

Tools and Handling 84

Good Pipetting Practice 85

Reagents and Reagent Mixes 86

ScreenTape Devices 87

Partially Used ScreenTape Devices 90

Sample Preparation 91

Ladder Considerations 92

Use the Correct Protocol 93

Mixing Recommendations 94

Centrifugation Recommendations 97

RNA Assays 99

Peak Integration 101

Peak Maxima Versus Average Molecular Weight Sizing and Molarity 104

Sizing Range 106

Quantitative range 107

Maximum Buffer Concentration in Sample 110

RINe Functional Range 112

8 Instrument Diagnosis 113

Instrument Diagnosis 114

Tests 115

Reporting 122

Agilent 4150 TapeStation System Manual 7

Contents

9 Maintenance 123

Maintenance Information 124

Exchange the Piercing Needle 127

Electrode Cartridge 130

Cleaning the Instrument 146

Preparing the Instrument for Transportation 147

Boxing for Transportation 153

Assembling Nest Covers 156

Insertion of the Lower Nest Cover 158

10 Troubleshooting 165

Software Messages 167

Troubleshooting DNA Applications 178

Troubleshooting RNA Applications 200

Instrument Communication 212

Collecting Troubleshooting Information 221

11 Appendix 223

Accessories Shipment List 224

8 Agilent 4150 TapeStation System Manual

Contents

Agilent 4150 TapeStation System Manual

Agilent Technologies

Safety

Symbols 10

Safety Messages 12

General Safety Information 13

This chapter provides information on safety symbols and safety messages.

10 Agilent 4150 TapeStation System Manual

Safety

Symbols

Table 1 Symbols

The apparatus is marked with this symbol when the user should refer to

the instruction manual in order to protect risk of harm to the operator and

to protect the apparatus against damage.

Indicates dangerous voltages.

Indicates a protected ground terminal.

Confirms that a manufactured product complies with all applicable

European Community directives. The European Declaration of Conformity

is available at:

http://regulations.corporate.agilent.com/DoC/search.htm

Manufacturing date.

Power symbol indicates On/Off.

The apparatus is not completely disconnected from the mains supply

when the power switch is in the Off position

Pacemaker

Magnets could affect the functioning of pacemakers and implanted heart

defibrillators.

A pacemaker could switch into test mode and cause illness. A heart

defibrillator may stop working. If you wear these devices keep at least

55 mm distance to magnets. Warn others who wear these devices from

getting too close to magnets.

Magnetic field

Magnets produce a far-reaching, strong magnetic field. They could

damage TVs and laptops, computer hard drives, credit and ATM cards,

data storage media, mechanical watches, hearing aids and speakers.

Keep magnets at least 25 mm away from devices and objects that could

be damaged by strong magnetic fields.

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