Agilent Technologies 7200 Manuale utente

Agilent Technologies

Agilent

7200 Accurate-Mass

Quadrupole

Time-of-Flight GC/MS

System

Operation Manual

Notices

No part of this manual may be reproduced in

any form or by any means (including elec-

tronic storage and retrieval or translation

into a foreign language) without prior agree-

ment and written consent from Agilent

Technologies, Inc. as governed by United

States and international copyright laws.

Manual Part Number

G3850-90005

Edition

First Edition, February 2012

Printed in USA

Agilent Technologies, Inc.

5301 Stevens Creek Blvd.

Santa Clara, CA 95051

Warranty

The material contained in this docu-

ment is provided “as is,” and is sub-

ject to being changed, without notice,

in future editions. Further, to the max-

imum extent permitted by applicable

law, Agilent disclaims all warranties,

either express or implied, with regard

to this manual and any information

contained herein, including but not

limited to the implied warranties of

merchantability and fitness for a par-

ticular purpose. Agilent shall not be

liable for errors or for incidental or

consequential damages in connec-

tion with the furnishing, use, or per-

formance of this document or of any

information contained herein. Should

Agilent and the user have a separate

written agreement with warranty

terms covering the material in this

document that conflict with these

terms, the warranty terms in the sep-

arate agreement shall control.

Technology Licenses

The hardware and/or software described in

this document are furnished under a license

and may be used or copied only in accor-

dance with the terms of such license.

Restricted Rights Legend

U.S. Government Restricted Rights. Soft-

ware and technical data rights granted to

the federal government include only those

rights customarily provided to end user cus-

tomers. Agilent provides this customary

commercial license in Software and

technical data pursuant to FAR 12.211

(Technical Data) and 12.212 (Computer Soft-

ware) and, for the Department of Defense,

DFARS 252.227-7015 (Technical Data -Com-

mercial Items) and DFARS 227.7202-3

(Rights in Commercial Computer Software

or Computer Software Documentation).

Safety Notices

CAUTION

A CAUTION notice denotes a

hazard. It calls attention to an oper-

ating procedure, practice, or the

like that, if not correctly performed

or adhered to, could result in

damage to the product or loss of

important data. Do not proceed

beyond a CAUTION notice until the

indicated conditions are fully

understood and met.

WARNING

A WARNING notice denotes a

hazard. It calls attention to an

operating procedure, practice, or

the like that, if not correctly per-

formed or adhered to, could result

in personal injury or death. Do not

proceed beyond a WARNING

notice until the indicated condi-

tions are fully understood and

met.

Operation Manual 3

About This Manual

This manual contains information for operating and

maintaining the Agilent 7200 Accurate-Mass Quadrupole

Time-of-Flight GC/MS system.

1 “Introduction”

Chapter 1 describes general information about the 7200 Q-TOF

GC/MS, including a hardware description, and general safety

warnings.

2 “Installing GC Columns”

Chapter 2 shows you how to prepare a capillary column for use

with the MS, install it in the GC oven, and connect it to the MS

using the GC/MS interface.

3 “Operating in Electron Impact (EI) Mode”

Chapter 3 describes routine operations in EI mode such as

setting temperatures, monitoring pressures, tuning, venting,

and pumpdown.

4 “Operating in Chemical Ionization (CI) Mode”

Chapter 4 describes additional tasks necessary to operate in CI

mode.

5 “Using the Removable Ion Source”

Chapter 5 shows you how to change the removable ion source.

6 “General Maintenance”

Chapter 6 describes general maintenance procedures for the

7200 Q-TOF GC/MS.

4 Operation Manual

Hardware User Information

Now your Agilent instrument documentation is in one place, at

your fingertips.

The hardware user information DVDs that ship with your

instrument provide an extensive collection of online help,

videos, and books for the Agilent 7890A GC, 7200 Q-TOF GC/MS,

7693 ALS, and the 7683B ALS. Included are localized versions of

the information you need most, such as:

• Getting Familiar documentation

• Safety and Regulatory guides

• Site Preparation checklists

• Installation information

• Operating guides

• Maintenance information

• Troubleshooting details

Operation Manual 5

Contents

1 Introduction

Abbreviations Used 10

The 7200 Accurate-Mass Quadrupole Time-of-Flight GC/MS

System 12

Hardware Description 15

Important Safety Warnings 16

Safety and Regulatory Certifications 20

Intended Use 23

Cleaning/Recycling the Product 23

Liquid Spills 23

Moving or Storing the MS 23

2 Installing GC Columns

Columns 26

To Prepare a Capillary Column for Installation 29

To Install a Capillary Column in a Split/Splitless Inlet 31

To Condition a Capillary Column 33

To Install a Capillary Column in the GC/MS Interface 35

To Prepare the Column Ends for a CFT Fitting 38

3 Operating in Electron Impact (EI) Mode

Operating the MS from the Data System 44

The GC/MS Interface 45

Before You Turn On the MS 47

Pumping Down 48

6 Operation Manual

Controlling Temperatures 48

Controlling Column Flow 48

Controlling Collision Cell Flow 49

Venting the MS 50

Typical Vacuum Pressures in EI Mode 51

To Set Monitors for Temperature and Vacuum Status 52

To Set the MS Analyzer Temperature 55

To Set the GC/MS Interface Temperature from the MassHunter

Workstation 57

To Calibrate the Column 58

To Configure the Collision Cell Gas 60

To Set the Collision Cell Gas Flow Rate 61

To Autotune the MS for EI Mode 62

To Remove the Upper RIS Cover 64

To Open the Analyzer Cover for Access to the Analyzer

Sideplate 65

To Pump Down the MS 66

To Vent the MS 69

4 Operating in Chemical Ionization (CI) Mode

Setting Up Your MS to Operate in CI Mode 72

The CI GC/MS Interface 73

Operating the CI MS 75

High Vacuum Pressure in CI Mode 76

Other Reagent Gases 78

CI Autotune 80

Operation Manual 7

The Flow Control Module 82

To Switch from the EI Mode to CI Mode 83

To Operate the Reagent Gas Flow Control Module 85

To Set a Reagent Gas Flow 87

To Perform a CI Autotune 88

5 Using the Removable Ion Source

The Removable Ion Source 92

To Change the Ion Source 94

To Install the RIS Probe Extraction Tool 97

To Remove the Source from the Analyzer Chamber 99

To Change the Ion Source on the RIS Probe Extraction Tool 101

To Install the Ion Source in the Analyzer Chamber 103

To Remove the RIS Probe Extraction Tool from the

Instrument 104

6 General Maintenance

Before Starting 106

Maintaining the Vacuum System 111

Maintaining the Analyzer 112

To Disassemble the EI Ion Source 114

To Assemble the EI Ion Source 116

To Disassemble the CI Ion Source 118

To Assemble the CI Ion Source 120

To Clean the Ion Source 122

To Remove a Filament 126

To Install a Filament 128

8 Operation Manual

Agilent Technologies

Agilent 7200 Accurate-Mass Quadrupole Time-of-Flight GC/MS System

Operation Manual

Introduction

Abbreviations Used 10

The 7200 Accurate-Mass Quadrupole Time-of-Flight GC/MS System 12

Hardware Description 15

Important Safety Warnings 16

Safety and Regulatory Certifications 20

Intended Use 23

Cleaning/Recycling the Product 23

Liquid Spills 23

Moving or Storing the MS 23

This section provides general information about the 7200

Accurate-Mass Quadrupole Time-of-Flight (Q-TOF) GC/MS

System, including a hardware description, and general safety

warnings.

10 Operation Manual

1 Introduction

Abbreviations Used

The abbreviations in Table 1are used in discussing this

product. They are collected here for convenience.

Table 1 Abbreviations

Abbreviation Definition

AC Alternating current

ALS Automatic liquid sampler

BFB Bromofluorobenzene (calibrant)

CC Collision cell

CI Chemical ionization

DC Direct current

DFTPP Decafluorotriphenylphosphine (calibrant)

EI Electron impact

EPC Electronic pneumatic control

eV Electron volt

GC Gas chromatograph

id Inside diameter

LAN Local Area Network

m/z Mass to charge ratio

MFC Mass flow controller

MS Mass spectrometer

NCI Negative chemical ionization

OFN Octafluoronaphthalene (sample)

PCI Positive chemical ionization

PFDTD Perfluoro-5,8-dimethyl-3,6,9-trioxydodecane (calibrant)

PFTBA Perfluorotributylamine (calibrant)

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