Agilent Technologies MX3000P Guida di sicurezza

Mx3000P and Mx3005P QPCR Systems

Setup and User’s Guide

Mx3000P and Mx3005P

QPCR Systems

SETUP AND USER’S GUIDE

IN #70225 J.1

For Research Use Only. Not for Use in Diagnostic Procedures.

LIMITED PRODUCT WARRANTY

Agilent warrants to the original purchaser that this product is free from defects in workmanship or

materials for a period of one (1) year from the date of purchase, as indicated on the original invoice,

under normal use.

The sole and exclusive remedy under this limited warranty is repair or replacement of defective

products or parts thereof. Repair or replacement products or parts thereof will be furnished solely on

an exchange basis and are obtainable only by the original purchaser. The original purchaser shall

return the defective product, or part thereof, properly packaged, postage or shipping costs prepaid to

Agilent. Loss or damage during shipment shall be at the risk of the purchaser.

This limited warranty does not include repair or replacement necessitated by accident, neglect,

misuse, relocation, unauthorized repair or modification of the product.

THE ABOVE LIMITED WARRANTY IS THE SOLE WARRANTY PROVIDED BY

AGILENT. NO OTHER WARRANTIES, EXPRESS OR IMPLIED, INCLUDING

WARRANTIES OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE,

ARE PROVIDED WHATSOEVER. NO AGILENT AGENT OR EMPLOYEE MAY

MODIFY, EXTEND OR ADD TO THE ABOVE LIMITED WARRANTY.

In no event will Agilent be liable for any direct, indirect, consequential or incidental damages,

including lost profits, or for any claim by any third party, arising out of the use, the results of use or

the inability to use this product.

Mx3000P and Mx3005P QPCR Systems

Setup and User’s Guide

Mx3000P and Mx3005P QPCR Systems Components Provided...................................................1

Setting Up the System.........................................................................................................................2

Locating the Instrument......................................................................................................... 2

Environmental Conditions for Use........................................................................................2

Unpacking the Instrument .....................................................................................................3

Installing the Fuse..................................................................................................................4

Connecting the Instrument to the PC.....................................................................................6

Configuring the PC and Installing the MxPro QPCR Software............................................7

Performing the Instrument Qualification Test Run.............................................................15

Hardware Information and Specifications .....................................................................................20

Hardware Specifications......................................................................................................20

Cautions and Warnings/Précautions et Avertissements ......................................................20

Instrument Status Indicator LED’s...................................................................................... 22

Sample Loading.................................................................................................................................23

Thermal Block Hardware....................................................................................................23

Consumables .......................................................................................................................24

Use of the Perfect Fit Frame with Single Tubes and Strip Tubes .......................................24

Application of Caps.............................................................................................................26

Maintaining the Instrument.............................................................................................................27

Replacement Parts............................................................................................................... 27

Changing the Lamp ............................................................................................................. 27

Replacing the Fuse ..............................................................................................................28

Cleaning the Instrument ......................................................................................................28

Troubleshooting ................................................................................................................................29

Software License and Notices...........................................................................................................34

MxPro QPCR Software License Agreement.......................................................................34

Trademarks..........................................................................................................................36

Mx3000P and Mx3005P QPCR Systems Support..........................................................................37

MX3000P AND MX3005P QPCR SYSTEMS

COMPONENTS PROVIDED

Components Quantity

Mx3000P or Mx3005P QPCR system 1

Power cord 1

Fuse holder kit 1

Notebook or desktop computer with Windows®Vista (or equivalent) and

Office 2007 (or equivalent)a

Serial-to-USB adapter 1

USB driver installation CD 1

Mouse 1

RS-232 serial null modem cable (part #22316) 1

Perfect Fit frame (catalog #401421) 1

Mx3000P/Mx3005P 96-well non-skirted PCR plates (catalog #410088) 5

Mx3000P/Mx3005P optical strip caps (catalog #401425) 60 strips of 8 caps

MxPro QPCR software installation CD 1

Installation qualification test plateband plate setup instructions 1

Setup and User’s Guide 1

Installation wall chart 1

Introduction to Quantitative PCR: Methods and Application Guide 1

Warranty card 1

aThe MxPro QPCR software is compatible with Windows®XP and Windows®Vista operating systems.

bStore the qualification test plate at –20°C upon receipt.

Mx3000P and Mx3005P QPCR Systems Setup and User’s Guide 1

SETTING UP THE SYSTEM

Important Retain the shipping container and all packaging materials, including the wooden

pallet. In the event that the instrument needs to be returned for service, the instrument

must be shipped in the original container to maintain warranty protection.

Locating the Instrument

Select a location for the Mx3000P or Mx3005P instrument according to the

following guidelines:

•Select a flat, level surface

•Ensure that the workbench is capable of supporting at least 45 lbs

(20.4 kg)

•Allow 4 inches (10.2 cm) airflow on the front and rear of the instrument

•Position the instrument to allow easy access to the instrument power

entry module (containing the instrument power switch)

•The instrument should be installed in a location where it will not be

exposed to direct sunlight or vibration during use

Environmental Conditions for Use

•Maximum altitude: 2000 m (6562 ft)

•Ambient temperature and relative humidity range for operation:

18–35°C (65–95°F); up to 85% non-condensing humidity

•Installation Category II

Installation Category ratings establish standardized voltage levels

that may occur in the electrical distribution system. Category II is

for measurements performed on circuits directly connected to the

electrical distribution system (i.e. a wall outlet on normal single-

phase supply [115/230 VAC]).

•Pollution Degree 2

Pollution Degree ratings define the type of environment the

equipment is typically installed in. Pollution Degree 2 refers to an

office environment with occasional condensation.

•For Indoor Use Only

2 Mx3000P and Mx3005P QPCR Systems Setup and User’s Guide

Unpacking the Instrument

Note To ensure safety, we recommend that the lifting and unpacking of

the system be performed by two people.

1. Remove the instrument from the shipping container. Do not apply force

to any part of the instrument directly; instead apply force to the plastic

bag or foam supports surrounding the instrument.

Important Retain the shipping container and all packaging

materials, including the wooden pallet. In the event that

the instrument needs to be returned for service, the

instrument must be shipped in the original container to

maintain warranty protection.

2. Remove the foam and cardboard supports and the plastic bag, then

place the instrument in an appropriate location (see Locating the

Instrument).

3. Remove the tape securing the instrument door from the instrument

front.

4. Open the instrument door by pushing up on the door handle. Remove

and retain the foam used to immobilize the scanning-head on the track.

5. Move the scan arm to the left of the thermal block top.

6. To access the thermal block, unlatch the heated lid by pulling forward

on the handle and then rotating the lid up and away from the thermal

block. Gently push down on the handle to lock the lid into the open

position. To close the lid on the thermal block, gently pull up on the

handle to release. After rotating the lid back to the closed position,

gently push the handle toward the back of the instrument to lock it into

the closed position.

7. Surrounding the 96-well thermal block is a plastic Perfect Fit frame.

Remove and retain the Perfect Fit frame for later use. See the Sample

Loading section for more information about the Perfect Fit frame.

Note Par mesure de sécurité, nous recommandons

d’être deux

personnes pour soulever et pour déballer l’instrument.

Mx3000P and Mx3005P QPCR Systems Setup and User’s Guide 3

Installing the Fuse

Important Do not turn on the instrument until you have installed the

fuse holder. The fuse is required for proper instrument

operation.

1. Remove the label located over the power entry module on the back of

the instrument just above the power switch.

2. Use a small flathead screwdriver or similar tool to open the fuse box.

3. Unpack the fuse holder from its shipping container.

4 Mx3000P and Mx3005P QPCR Systems Setup and User’s Guide

4. The flat edge of the fuse holder has two different voltage labels (115V

and 230V) printed in opposite orientation to each other. With the voltage

labels facing out, insert the fuse holder into the fuse box so that the label

for the desired voltage is in the proper orientation. (If your instrument is

100-120 volts, insert the fuse holder such that the 115V label is right-

side-up. If your instrument is 200-240 volts, insert the fuse holder such

that the 230V label is right-side-up.)

5. Replace the cover on the fuse box. Verify that the correct voltage label

is visible through the cover.

Voltage label

Mx3000P and Mx3005P QPCR Systems Setup and User’s Guide 5

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