Agilent Technologies 1290 Infinity II 2D-LC Solution Manuale utente

Agilent 1290 Infinity II 2D-LC Solution

MassHunter Acquisition for TOF and Q-TOF

User Guide

2D-LC User Guide

Notices

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Document No: D0004828 Rev. A

Edition: 05/2021

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2D-LC User Guide 3

In This Book

This manual covers the Agilent 1290 Infinity II 2D-LC Solution for MassHunter

TOF and Q-TOF.

1Introduction

This chapter describes the product of Agilent 1290 Infinity II 2D-LC Solution.

2 Concepts of 2D-LC

This chapter describes the concepts of Agilent 1290 Infinity II 2D-LC Solution.

3 Compatibility Matrix

This chapter provides information about installation and execution prerequisites

regarding hardware, firmware, and the operating system. Agilent 1290 Infinity II

2D-LC Solution.

4 Installation

This chapter describes the hardware and software installation of the Agilent

1290 Infinity II 2D-LC Solution. The 2D-LC instrument can be used with the

software described in this document. The installation instructions are valid for

the modes standard heart-cutting, multiple heart-cutting, high resolution

sampling and comprehensive 2D-LC.

5 2D-LC Data Acquisition in MassHunter Workstation 11

This chapter provides information about 2D-LC data acquisition in MassHunter

Workstation 11.

6MethodParameters

This chapter provides background information on method parameters. It helps to

optimize methods in Agilent 1290 Infinity II 2D-LC Solution in the modes standard

heart-cutting, multiple heart-cutting, high resolution sampling and

comprehensive 2D-LC.

In This Book

2D-LC User Guide 4

7 Method Development of Active Solvent Modulation (ASM)

This chapter provides information on how to develop methods when using Active

Solvent Modulation (ASM).

8 Run the System

This chapter describes how to run the Agilent 1290 Infinity II 2D-LC Solution in

the modes standard heart-cutting, multiple heart-cutting, high resolution

sampling and comprehensive 2D-LC with the driver-based 2D-LC Solution.

9 Data Analysis

This chapter provides information on how to analyze 2D-LC data with software.

10 Troubleshooting and Diagnostics

This chapter gives an overview about the troubleshooting and diagnostic

features and the different user interfaces.

11 Error Information

This chapter describes the meaning of error messages, and provides information

on probable causes and suggested actions how to recover from error conditions.

12 Maintenance

This chapter describes the maintenance of the 2D-LC Solution.

13 Parts for Maintenance

This chapter provides information on parts material required for the solution.

14 Theoretical Background

This chapter gives the theoratical background of 2D-LC and describes the

system components (soft- and hardware) of the Agilent 1290 Infinity II 2D-LC

Solution.

15 Appendix

This chapter provides addition information on safety, legal and web.

2D-LC User Guide 5

Contents

1Introduction10

Product Description 11

Features 12

Terms related to 2D-LC 14

2 Concepts of 2D-LC 15

Concepts of 2D-LC 16

Heart-Cutting 2D-LC (LC-LC) 18

Multiple Heart-Cutting and High Resolution Sampling 2D-LC 19

Comprehensive 2D-LC (LCxLC) 30

Triggering of 2D-LC 32

Active Solvent Modulation (ASM) 35

3 Compatibility Matrix 45

Supported Chromatographic Data Systems 46

Supported Drivers 47

Supported Operating Systems 48

Supported Firmware 49

Available Languages 50

PC Requirements 51

Licensing 52

4 Installation 53

Hardware Installation 54

Software Installation 89

2D-LC User Guide 6

5 2D-LC Data Acquisition in MassHunter Workstation 11 103

Start the Data Acquisition Software 104

Overview 2D-LC in MassHunter Acquisition 11 106

Instrument Status 107

2D-LC User Interface 108

2D-LC Valves Online Monitor in the 2D-LC User Interface 114

Method Editor Window 116

Sample Run Window 117

Worklist Window 118

Tune Window 119

Instrument Details 120

Log book in MassHunter Acquisition 11 123

6 Method Parameters 125

Method Editor 2D-LC 126

Set the 2D-LC Method parameters 127

Preview (2D-LC) 154

Set up a Peak-Based Experiment Graphically 168

Setup 2D Gradient Graphically 173

Setup Second Dimension Gradient with the Graphical User Interface 175

Additional Information 177

7 Method Development of Active Solvent Modulation (ASM) 185

Method Development of Active Solvent Modulation (ASM) 186

Method Parameters 187

Optimize the Dilution by Using ASM Capillaries 188

Optimize the Sample Loop Flush 189

Include the ASM Phase to the 2D Gradient 190

Optimize Dilution Through Method Settings 191

2D-LC User Guide 7

8 Run the System 192

Familiarization to Start a System Run 193

Prepare the 2D-LC System 194

Configure the 2D-LC System 195

Checkout Familiarization Procedure 198

Prepare the Experiment 199

Run the Experiment 201

9 Data Analysis 229

2D-LC Data Analysis/Data Evaluation for MassHunter 230

GC Image Basic Information 256

10 Troubleshooting and Diagnostics 275

Overview of the Module’s Indicators and Test Functions 276

User Interfaces 277

Agilent Lab Advisor Software 278

Lab Advisor Instrument Control 280

Lab Advisor Service &Diagnostic 286

The Basic Principle of Troubleshooting 294

Recommended Tests to Conclude Troubleshooting 304

11 Error Information 305

What Are Error Messages 306

General Error Messages 307

Module-Specific Error Messages 313

2D-LC User Guide 8

12 Maintenance 317

Introduction to Maintenance 318

Warnings and Cautions 319

Overview of Maintenance 321

Cleaning the Module 322

Correcting Leaks 323

Replace Valve Heads 324

Replacing Parts of the Valve Head 330

Replacing the Fuses of the Infinity Valve Drive 332

13 Parts for Maintenance 334

2D-LC Loops 335

2D-LC Capillaries 336

ASM Capillaries 337

Pressure Release Kit 338

2D-LC Easy Starter Kit 339

Valve Drive Parts 340

Valve Driver Parts Infinity II 341

Valve Head Parts 342

Vale Options Overview (for 2D-LC) 344

MS Diverter Valve 348

Valve Options Overview (for G7116B) 350

Additional Heater Devices 352

Accessories and Consumables (for G7116B) 354

InfinityLab Quick Connect and Quick Turn Fittings 356

Capillary Kits 360

Important Customer Web Links 361

2D-LC User Guide 9

14 Theoretical Background 362

Theoretical basis of 2D-LC 363

2D as detector 371

Successful Mode Combinations 373

Solvent Elution Modes 374

Practical Issues 380

15 Appendix 382

General Safety Information 383

Waste Electrical and Electronic Equipment (WEEE) Directive 389

Radio Interference 390

Sound Emission 391

Solvent Information 392

Further Information 393

Agilent Technologies on Internet 394

2D-LC User Guide 10

1Introduction

Product Description 11

Features 12

Terms related to 2D-LC 14

This chapter describes the product of Agilent 1290 Infinity II 2D-LC Solution.

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